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Fortification of yogurt with oyster hydrolysate and evaluation of its in vitro digestive characteristics and anti-inflammatory activity

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Food Bioscience 2022年第3期47卷 889-897页

作者： Li Liu Suisui Jiang Wei Xie Jinjin Xu yuanhui zhao Mingyong Zeng College of Food Science and Engineering Ocean University of ChinaQingdaoShandong Province266003China

In this study,goat and cow yogurts fortified with oyster hydrolysate(OTH)were produced to evaluate gastrointestinal digestion,digestive characterization,and bioactivities.288 peptides were identified in the oyster goat yogurt(OGY)digestates and 17 peptides were predicted to be associated with the regulation of gastrointestinal mucosal ***,the digestate fractions of molecular weight(MW)<10 kDa showed high cellular antioxidant activity and anti-inflammatory activity as demonstrated in Caco-2 and HT-29 cell *** peptide fractions(MW<10 kDa)exhibited cellular anti-inflammatory activity as demonstrated in suppression of LPS-induced TNF-α,IL-1β,and IL-6 *** Caco-2 and HT-29 cell lines pretreated with 500μg/mL OGY peptide fractions(MW<10 kDa)illustrated the expression inhibition of NF-κB p65 *** overall results suggested that OGY offered a promising functional *** work provides some insights for the oyster high value utilization and goat dairy development.

关键词： Oyster Goat yogurt Digestibility Peptide Inflammation

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蒸制鲟鱼背部肉脂质氧化特性及风味品质评价

食品工业科技 2024年第16期45卷 13-24页

作者：李珂谢伟徐新星刘荔李雪飞白帆汪金林赵元晖中国海洋大学食品科学与工程学院山东青岛266404 中国海洋大学三亚海洋研究院海南三亚572024 衢州鲟龙水产食品科技开发有限公司浙江衢州324002

探究了蒸制时间对鲟鱼背部肉脂质氧化及风味的影响。本文通过测定过氧化值、酸价、丙二醛和共轭烯烃表征了鱼肉蒸制过程中脂质氧化的程度,测定脂肪酸含量变化分析了蒸制对挥发性化合物和营养品质的影响,并通过非靶向脂质组学探究了蒸制... 详细信息

探究了蒸制时间对鲟鱼背部肉脂质氧化及风味的影响。本文通过测定过氧化值、酸价、丙二醛和共轭烯烃表征了鱼肉蒸制过程中脂质氧化的程度,测定脂肪酸含量变化分析了蒸制对挥发性化合物和营养品质的影响,并通过非靶向脂质组学探究了蒸制鲟鱼肉中脂质组成的变化,最后通过热氧化模型验证了磷脂对鲟鱼背部肉风味产生的具体影响。结果表明,蒸制16 min的鲟鱼背部肉脂质氧化程度适中,不饱和脂肪酸含量丰富,营养价值高。脂质适度氧化有利于特征风味的形成,而磷脂是挥发性风味化合物的主要前体物,其中亚油酸(C18:2)、DHA(C22:6)、油酸(C18:1)和花生四烯酸(C20:4)是与磷脂结合的主要不饱和脂肪酸。此外,模拟蒸制条件的结果表明磷脂氧化出现大量的吡嗪和呋喃类化合物,为蒸制鲟鱼背部肉贡献了一定的焙烤香气。综上,脂质的适度氧化有利于提高蒸制鲟鱼背部肉的风味和营养品质,磷脂氧化对蒸制鲟鱼背部肉风味形成具有重要影响,本研究可为鲟鱼肉风味提升提供一定的理论依据。

关键词：鲟鱼蒸制磷脂脂质氧化风味品质

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2021—2022年北京体育大学新生高尿酸血症患病情况及相关因素

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卫生研究 2024年第4期53卷 592-599,607页

作者：于文朗王雯星赵元慧林芳萍张世超彭佳乐葛永伟陈彦仲任弘北京体育大学运动人体科学学院北京100084 运动与体质健康教育部重点实验室北京100084

目的了解北京体育大学新生高尿酸血症患病情况,探讨大学生人群高尿酸血症的相关因素。方法于2021年9月—2022年2月选取北京体育大学3372名新生作为研究对象,进行非同日的2次血尿酸检测,计算人群患病率并使用病例-对照的方法探讨大学生... 详细信息

目的了解北京体育大学新生高尿酸血症患病情况,探讨大学生人群高尿酸血症的相关因素。方法于2021年9月—2022年2月选取北京体育大学3372名新生作为研究对象,进行非同日的2次血尿酸检测,计算人群患病率并使用病例-对照的方法探讨大学生群体高尿酸血症危险因素,以方便抽样的方法从该人群的高尿酸血症患者中抽取246人纳入病例组,从非高尿酸血症者中如抽取211人纳入对照组,进行体格检查和实验室检查,并进行回顾性的问卷调查,包括年龄、性别、专业、生源地等一般情况和高尿酸血症相关饮食、高尿酸血症疾病认知、体力活动水平、睡眠情况等内容。采用卡方分析、单因素Logistic回归分析及多因素Logistic回归分析进行统计分析。结果非同日2次血尿酸检测实际确诊高尿酸血症患者人数为479人(14.21%);其中男性391人(22.39%),女性88人(5.41%)。病例-对照研究共纳入457名研究对象,其中病例组246人(男性218人,女性28人,年龄19.74岁),对照组211人(男性177人,女性34人,年龄19.93岁),2组受试者在性别和年龄分布上差异无统计学意义。单因素Logistic回归分析结果表明中心性肥胖(OR=31.52,95%CI 7.59~130.86)、肥胖(OR=2.59,95%CI 1.20~5.58)、超重(OR=1.67,95%CI 1.08~2.59)、经常食用新鲜蔬菜(OR=0.66,95%CI 0.43~0.99)以及每日饮水1500~2000 mL(OR=0.63,95%CI 0.41~0.95)与高尿酸血症有关;将以上因素进行多因素Logistic回归分析,最终中心性肥胖(OR=32.05,95%CI 7.65~134.20)、肥胖(OR=3.22,95%CI 1.44~7.20)以及每日饮水1500~2000 mL(OR=0.60,95%CI 0.37~0.95)在P=0.05的水平上被模型纳入。结论该大学新生高尿酸血症患病率较高,其中男性患病情况更普遍。肥胖以及中心性肥胖是大学生群体高尿酸血症的危险因素,每日饮水量1500~2000 mL是保护因素。

关键词：高尿酸血症青年人群大学生病例-对照研究

Effects of protein oxidation, cathepsins, and various freezing temperatures on the quality of superchilled sturgeon fillets

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Marine Life Science & Technology 2022年第1期4卷 117-126页

作者： Nana zhao Xianqing Yang Yujin Li Haohao Wu Yiping Chen Ruichang Gao Feng Xiao Fan Bai Jinlin Wang Zunying Liu Xin Gao yuanhui zhao College of Food Science and Engineering Ocean University of ChinaQingdao266003China South China Sea Fisheries Institute Chinese Academy of Fishery SciencesGuangzhou510641China School of Food and Bioengineering Jiangsu UniversityZhenjiang212013China College of Food and Bioengineering Henan University of Science and TechnologyLuoyang471023China Hangzhou Qiandaohu Sturgeon Technology Co. LtdHangzhou310000China

Many aquatic products have been stored using superchilling technology, but rarely used for the storage of sturgeon fillets. In this study, we investigated the effects of protein oxidation, cathepsin, and various freezing temperatures on the quality of superchilled sturgeon fillets. Sensory evaluation results showed that the sensory attributes of superchilled (−3 °C) sturgeon fillets were acceptable three times longer (18 days) than samples stored at refrigeration temperatures (4 °C). The sarcoplasmic protein, carbonyl, myofibrillar protein, total sulfhydryl content and the surface hydrophobicity were determined using fluorescence spectrophotometry and SDS-PAGE. Results showed that superchilling might protect myofibrillar proteins from oxidation compared to refrigeration temperatures. The activity of the three cathepsins (B, L, and H) in terms of myofibrillar, mitochondria, lysosomes, and sarcoplasm demonstrated that superchilling can inhibit cathepsins activity in sturgeon and protect its muscle structure. Microscopic observations showed that as the temperature decreased, the gap area of the muscle fibers decreased, and the deformation of cross-sectional slices was gradually reduced. In addition, the freezing rate of ice crystals produced during the freezing process influenced the muscle structure, texture, and sensory attributes. Superchilled sturgeon fillets showed good hardness, chewiness, and water retention. In conclusion, superchilling technology shows promise for its ability to extend the shelf life while maintaining the texture and sensory attributes of fresh sturgeon fillets.

关键词： Sturgeon Superchilling Protein oxidation Cathepsin Microstructure

Heavy metal pollution in intertidal sediments from Quanzhou Bay, China

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Journal of Environmental Sciences 2008年第6期20卷 664-669页

作者： YU Ruilian YUAN Xing zhao yuanhui HU Gongren TU Xianglin Department of Environmental Science Northeast Normal University Changchun 130024 China Department of Environmental Science and Engineering Huaqiao University Quanzhou 362021 China Guangzhou Institute of Geochemistry Chinese Academy of Sciences Guangzhou 510640 China

The concentrations of eight heavy metals （Cu, Zn, Pb, Cd, Cr, Ni, Hg, and As） in the intertidal surface sediments from Quanzhou Bay were determined to evaluate their levels and spatial distribution due to urbanization and economic development of Quanzhou region, southeast China. The ranges of the measured concentrations in the sediments are as follows： 24.8-119.7 mg/kg for Cu, 105.5-241.9 mg/kg for Zn, 34.3-100. 9 mg/kg for Pb, 0.28-0. 89 mg/kg for Cd, 51.1-121.7 mg/kg for Cr, 16.1-45.7 mg/kg for Ni, 0.17-0.74 mg/kg for Hg, and 17.7-30.2 mg/kg for As. The overall average concentrations of above metals exceed the primary standard criteria but meet the secondary standard criteria of the Chinese National Standard of Marine Sediment Quality. Several contents of Cu and Hg exceed the secondary standard criteria at some stations. The results of geoaccumulation index （Igeo） show that Cd causes strong pollution in most of the study area. There are no significant correlations among most of these heavy metals, indicating they have different anthropogenic and natural sources. Some locations present severe pollution by heavy metals depending on the sources, of which sewage outlets, aquatic breeding, and commercial ports are the main sources of contaminants to the area.

关键词： heavy metal intertidal sediment contamination geoaccumulation index Quanzhou Bay

Toxicity of substituted benzenes and algae (Scenedesmus obliquus) with solvation equation

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Chinese Science Bulletin 2009年第10期54卷 1690-1696页

作者： zhao yuanhui QIN WeiChao SU LiMin YUAN Xing LU GuangHua ABRAHAN H. Michael Department of Environmental Science Northeast Normal University Changchun 130024 China College of Environmental Science and Engineering Hohai University Nanjing 210098 China Department of Chemistry University College London London WC1H OA J UK

Quantitative structure-activity relationships (QSARs) for the toxicity of substituted phenols, anilines and nitrobenzenes to algae (Scenedesmus obliquus) have been studied. Chemicals were divided into polar narcotics and electrophiles based on structure criteria according to the toxicity mechanisms of action. A good relationship between the toxicity and octanol/water partition coefficient (logP) was observed for the polar narcotic compounds. The results confirmed that hydrophobicity is the determining physicochemical parameter in modelling narcotics. However, no statistically significant mono-parametric QSAR models could be made from descriptors calculated in this study for all the compounds. In order to investigate the mechanisms of toxic action and develop QSAR models for all compounds, Abraham solvation descriptors were calculated from the program of Pharma-Algorithms. Stepwise regression produced a three-parameter QSAR model (polarizability/dipolarity, hydrogen-bond basicity and molecular volume) with a good statistical fit for all the compounds studied, which had square of the correlation coefficient R2=0.83. We have also developed an interpretable QSAR model by using parameter of logP, which represents the bio-uptake process, and polarizability/dipolarity (S), which represents the interaction of chemicals with the acceptor micromolecules (target receptor) at the active site. No hydrogen-bond interaction was observed between the chemicals and receptor micromolecules.

关键词：毒性作用取代苯酚溶剂化硝基苯类化合物藻类定量构效关系氢键相互作用方程

Study on the Free Radical Scavenging Activity of Sea Cucumber (Paracaudina chinens var.) Gelatin Hydrolysate

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Journal of Ocean University of China 2007年第3期6卷 255-258页

作者： ZENG Mingyong XIAO Feng zhao yuanhui LIU Zunying LI Bafang DONG Shiyuan College of Food Science and Engineering Ocean University of China Qingdao 266003 P. R. China

Gelatin from the sea cucumber(Paracaudina chinens var.) was hydrolyzed by bromelain and the hydrolysate was found to have a high free radical scavenging activity. The hydrolysate was fractionated through an ultrafiltration membrane with 5 kDa molecular weight cutoff(MWCO). The portion(less than 5 kDa) was further separated by Sephadex G-25. The active peak was col-lected and assayed for free radical scavenging activity. The scavenging rates for superoxide anion radicals(O2·-) and hydroxyl radi-cals(·OH) of the fraction with the highest activity were 29.02% and 75.41%,respectively. A rabbit liver mitochondrial free radical damage model was adopted to study the free radical scavenging activity of the fraction. The results showed that the sea cucumber gelatin hydrolysate can prevent the damage of rabbit liver and mitochondria.

关键词： Paracaudina chinens var. gelatin hydrolysate free radical scavenging activity mitochondria

Automatic modeling algorithm of stochastic error for inertial sensors

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Control Theory and Technology 2024年第1期22卷 81-91页

作者： Luodi zhao Long zhao School of Automation Science and Electrical Engineering Beihang UniversityBeijing100191China Digital Navigation Center Beihang UniversityBeijing100191China Science and Technology on Aircraft Control Laboratory Beihang UniversityBeijing100191China

This paper proposes an automatic algorithm to determine the properties of stochastic processes and their parameters for inertial error. The proposed approach is based on a recently developed method called the generalized method of wavelet moments (GMWM), whose estimator was proven to be consistent and asymptotically normally distributed. This algorithm is suitable mainly (but not only) for the combination of several stochastic processes, where the model identification and parameter estimation are quite difficult for the traditional methods, such as the Allan variance and the power spectral density analysis. This algorithm further explores the complete stochastic error models and the candidate model ranking criterion to realize automatic model identification and determination. The best model is selected by making the trade-off between the model accuracy and the model complexity. The validation of this approach is verified by practical examples of model selection for MEMS-IMUs (micro-electro-mechanical system inertial measurement units) in varying dynamic conditions.

关键词： GMWM Stochastic process Inertial sensor Sensor calibration Error model Allan variance

Meeting the challenges of invasive alien species

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National Science Review 2024年第3期11卷 296-298页

作者： Weijie zhao NSR news

The Xinjiang Uygur Autonomous Region of China is famous for its tomato production. Abundant sunshine, large day–night temperature difference and the abundant irrigation by snowmelt from the Tianshan Mountains produce...

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