检索结果-南通市图书馆

Porcine circovirus type 2 capsid protein induces unfolded protein response with subsequent activation of apoptosis

在线全文

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2017年第4期18卷 316-323页

作者： Ying-shan ZHOU yuan-xing gu Bao-zhu QI Yi-kai ZHANG Xiao-liang LI Wei-huan FANG College of Animal Science and Technology Zhejiang Provincial Engineering Laboratory for Animal Health Inspection & Internet TechnologyZhejiang A&F University Institute of Preventive Veterinary Medicine Zhejiang University

Porcine circovirus type 2（PCV2）has recently been reported to elicit the unfolded protein response（UPR）via activation of the PERK/e IF2α（RNA-activated protein kinase-like endoplasmic reticulum（ER）kinase/eukaryotic initiation factor 2α）*** study attempted to examine which viral protein might be involved in inducing UPR and whether this cellular event would lead to apoptosis of the cells expressing the viral *** transient expression,we found that both replicase（Rep）and capsid（Cap）proteins of PCV2 could induce ER stress as shown by increased phosphorylation of PERK with subsequent activation of the eI F2α-ATF4（activating transcription factor 4）-CHOP（CCAAT/enhancer-binding protein homologous protein）*** expression,but not Rep,significantly reduced antiapoptotic B-cell lymphoma-2（Bcl-2）and increased caspase-3 cleavage,possibly due to increased expression of *** knockdown of PERK by RNA interference clearly reduced Cap-induced CHOP expression,caspase-3cleavage,and apoptotic cell death possibly by partially rescuing Bcl-2 expression,we propose that there is connection between Cap-induced UPR and apoptosis via the PERK/eI F2α/ATF4/CHOP/Bcl-2 *** study,together with our earlier studies,provides insight into the mechanisms underlying PCV2 pathogenesis.

关键词： Porcine circovirus 2 Capsid protein Unfolded protein response Apoptosis

Insight Into the Separation-of-Variable Methods for the Closed-Form Solutions of Free Vibration of Rectangular Thin Plates

在线全文

学校读者我要写书评

暂无评论

Computer Modeling in Engineering & Sciences 2025年第1期142卷 329-355页

作者： Yufeng xing Ye yuan Gen Li Institute of Solid Mechanics Beihang University(BUAA)Beijing100191China

The separation-of-variable(SOV)methods,such as the improved SOV method,the variational SOV method,and the extended SOV method,have been proposed by the present authors and coworkers to obtain the closed-form analytical solutions for free vibration and eigenbuckling of rectangular plates and circular cylindrical *** taking the free vibration of rectangular thin plates as an example,this work presents the theoretical framework of the SOV methods in an instructive way,and the bisection–based solution procedures for a group of nonlinear eigenvalue ***,the explicit equations of nodal lines of the SOV methods are presented,and the relations of nodal line patterns and frequency orders are *** is concluded that the highly accurate SOV methods have the same accuracy for all frequencies,the mode shapes about repeated frequencies can also be precisely captured,and the SOV methods do not have the problem of missing roots as well.

关键词： Separation-of-variable method Rayleigh quotient nodal line eigenvalue equation bisection method

Constructing quasi-2D perovskite to collect hot carriers to reduce open-circuit voltage loss for efficient inverted perovskite solar cells

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China Chemistry 2025年

作者： Lei gu xing Li Chao Wu Yang Feng Chunming Yang Lin Song Frontiers Science Center for Flexible Electronics Institute of Flexible Electronics Northwestern Polytechnical University Shanghai Synchrotron Radiation Facility Shanghai Advanced Research Institute Chinese Academy of Sciences

Inverted perovskite solar cells （PSCs） have received great attention owing to the high certificated power conversion efficiency（PCE） reaching 26.7%. However, interfacial charge recombination is far superior to perovskite bulk charge recombination due to the use of fullerenes and their derivatives in inverted perovskite solar cells, which induces devastating open circuit voltage（VOC） losses. For this reason the present work attempts to use 4-chloropiperidine hydrochloride for the upper surface modification of perovskite to construct a quasi-two-dimensional （2D） structure with suitable 2D layer widths （n=2, 3 dominant）,which has a smaller electron carrier transport barrier without sacrificing short-circuit current （JSC） in the device. This strategy effectively passivates the interfacial defect states while promoting energy level alignment to achieve carrier dissociation and equilibrium transport at the interface, which in turn resulted in improved fill factor （FF）. In addition, additional energy levels are provided by 2D perovskites to collect hot carriers, resulting in improved device open circuit voltage （VOC）. As a result, the device PCE increases from 22.37%to 24.11%after the 4CPD treatment. In addition, the 4CPD-treated PSCs maintain 90%of the initial PCE after storage in an N2atmosphere at 50°C for 1,080 h, whereas the control devices show a 15%PCE loss under the same aging conditions. This work provides new opportunities to suppress interfacial charge recombination in inverted devices.

关键词：

Exploration of Gas‑Dependent Self‑Adaptive Reconstruction Behavior of Cu_(2)O for Electrochemical CO_(2)Conversion to Multi‑Carbon Products

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Nano-Micro Letters 2025年第3期17卷 280-296页

作者： Chaoran Zhang Yichuan gu Qu Jiang Ziyang Sheng Ruohan Feng Sihong Wang Haoyue Zhang Qianqing Xu Zijian yuan Fang Song State Key Laboratory of Metal Matrix Composites School of Materials Science and EngineeringShanghai Jiao Tong UniversityShanghai 200240People’s Republic of China

Structural reconstruction of electrocatalysts plays a pivotal role in catalytic performances for CO_(2)reduction reaction(CO_(2)RR),whereas the behavior is by far superficially ***,we report that CO_(2)accessibility results in a universal self-adaptive structural reconstruction from Cu_(2)O to Cu@CuxO composites,ending with feeding gas-dependent microstructures and catalytic *** CO_(2)-rich atmosphere favors reconstruction for CO_(2)RR,whereas the CO_(2)-deficient one prefers that for hydrogen evolution *** the assistance of spectroscopic analysis and theoretical calculations,we uncover a CO_(2)-induced passivation behavior by identifying a reductionresistant but catalytic active Cu(I)-rich amorphous layer stabilized by*CO ***,we find extra CO production is indispensable for the robust production of *** inverse correlation between durability and FECO/FEC2H4 is disclosed,suggesting that the selfstabilization process involving the absorption of*CO intermediates on Cu(I)sites is essential for durable *** by this insight,we design hollow Cu_(2)O nanospheres for durable and selective CO_(2)RR electrolysis in producing *** work recognizes the previously overlooked passivation reconstruction and self-stabilizing behavior and highlights the critical role of the local atmosphere in modulating reconstruction and catalytic processes.

关键词： CO_(2)reduction reaction Electrocatalysts Cu_(2)O Reconstruction Self-adaptive electrocatalysis

Low‑Temperature Fabrication of Stable Black‑Phase CsPbI_(3)Perovskite Flexible Photodetectors Toward Wearable Health Monitoring

在线全文

学校读者我要写书评

暂无评论

Nano-Micro Letters 2025年第3期17卷 232-245页

作者： Yingjie Zhao Yicheng Sun Chaoxin Pei xing Yin Xinyi Li Yi Hao Mengru Zhang Meng yuan Jinglin Zhou Yu Chen Yanlin Song College of Chemistry and Pingyuan Laboratory Zhengzhou UniversityZhengzhou 450001People’s Republic of China Key Laboratory of Green Printing Institute of ChemistryChinese Academy of SciencesBeijing 100190People’s Republic of China Key Laboratory of Bio‑Inspired Materials and Interfacial Science Technical Institute of Physics and ChemistryChinese Academy of SciencesBeijing 100190People’s Republic of China Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049People’s Republic of China

Flexible wearable optoelectronic devices fabricated fromorganic–inorganic hybrid perovskites significantly accelerate the developmentof portable energy,biomedicine,and sensing fields,but their poor thermal stabilityhinders further ***,all-inorganic perovskites possessexcellent thermal stability,but black-phase all-inorganic perovskite filmusually requires high-temperature annealing steps,which increases energy consumptionand is not conducive to the fabrication of flexible wearable *** this work,an unprecedented low-temperature fabrication of stable blackphaseCsPbI3perovskite films is demonstrated by the in situ hydrolysis reactionof diphenylphosphinic chloride *** released diphenyl phosphateand chloride ions during the hydrolysis reaction significantly lower the phasetransition temperature and effectively passivate the defects in the perovskitefilms,yielding high-performance photodetectors with a responsivity of 42.1 AW−1 and a detectivity of 1.3×10^(14)***,high-fidelity imageand photoplethysmography sensors are demonstrated based on the fabricated flexible wearable *** work provides a newperspective for the low-temperature fabrication of large-area all-inorganic perovskite flexible optoelectronic devices.

关键词： In situ hydrolyzation Low-temperature processing All-inorganic perovskite Flexible photodetectors Health monitoring

Single-cell omics: experimental workflow, data analyses and applications

在线全文

学校读者我要写书评

暂无评论

Science China Life Sciences 2025年第1期68卷 5-102页

作者： Fengying Sun Haoyan Li Dongqing Sun Shaliu Fu Lei gu Xin Shao Qinqin Wang Xin Dong Bin Duan Feiyang xing Jun Wu Minmin Xiao Fangqing Zhao Jing-Dong J.Han Qi Liu Xiaohui Fan Chen Li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

A stretchable, ionic conductive, and adhesive patch electrode with ultra-low on-skin impedance for electrophysiological signal recording

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science China(Information Sciences) 2025年

作者： Yang LI Yuzhe gu Sheng QIAN Yuncong PANG Aoxi YU Shuwen ZHENG Wenjie XIA yuan LIAO Baoguang LIU Shujuan LIU Qiang ZHAO College of Electronic and Optical Engineering & College of Flexible Electronics (Future Technology) Nanjing University of Posts & Telecommunications (NJUPT) State Key Laboratory of Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors Institute of Advanced Materials (IAM) Nanjing University of Posts & Telecommunications (NJUPT)

Flexible electrodes have significant potential in fields such as healthcare and neuroscience, as they enable comprehensive recording and analysis of human electrical signals, providing deeper insights into physiological conditions and the state of internal organs and tissues [1, 2]. However, commercial Ag/AgCl wet electrodes face issues such as dehydration,poor comfort, and insufficient mechanical strength, while other types of electrodes, like dry and semi-dry electrodes,also have their own limitations, including the higher contact impedance. In contrast, ionically conductive electrodes offer potential advantages such as transparency, stability, and low impedance, making them a promising alternative to existing electrically conductive electrodes [3]. Acrylic acid (AA)and 2-acrylamido-2-methylpropanesulfonic acid (AMPS) are commonly used in the synthesis of ionic gels, as they ionize in water to produce a large number of ions, helping to maintain moisture balance and reduce impedance caused by dehydration [4]. However, the presence of freely moving ions in the gel network can interfere with conductivity, highlighting the need to introduce new conductive networks to enhance performance [5].

关键词：

一种用于数据库开发的UML类图的规格说明与C++源代码生成技术

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

上海大学学报（自然科学版） 2004年第1期10卷 43-51页

作者：杨越冯远兴顾训穰上海大学计算机工程与科学学院上海200072

该文分析了信息系统软件开发的特点,并在UML静态结构模型中类图的基础上,提出了一种针对信息系统软件开发,特别是数据库系统开发的规格说明语言MISFRAME的语法原型,详细阐明了其在数据库开发中的语法定义,提供了相应的代码生成器,并说... 详细信息

该文分析了信息系统软件开发的特点,并在UML静态结构模型中类图的基础上,提出了一种针对信息系统软件开发,特别是数据库系统开发的规格说明语言MISFRAME的语法原型,详细阐明了其在数据库开发中的语法定义,提供了相应的代码生成器,并说明了其生成源代码的原理.

关键词： UML 类图规格说明 MISFRAME 代码生成器

浑水入渗对粗粒土柱孔隙及渗透性影响试验研究

在线全文

学校读者我要写书评

暂无评论

岩土工程学报 2025年第1期47卷 173-183页

作者：毛海涛张超袁古城何涛谷易山西农业大学城乡建设学院山西太原030000 重庆三峡学院土木工程学院重庆404120

浑水渗流在水利工程的反滤设计、浑水浇灌、堤坝渗流控制等方面都有较大影响,目前理论多采用稳定渗流计算方法,忽略了渗流过程中粗细颗粒与水力的耦合作用。自制渗流装置,将不同控制粒径浑水、不均匀系数粗粒土柱和水头等作为基本控制因... 详细信息

浑水渗流在水利工程的反滤设计、浑水浇灌、堤坝渗流控制等方面都有较大影响,目前理论多采用稳定渗流计算方法,忽略了渗流过程中粗细颗粒与水力的耦合作用。自制渗流装置,将不同控制粒径浑水、不均匀系数粗粒土柱和水头等作为基本控制因素,研究不同工况下浑水中悬浮颗粒对粗粒土土柱中的渗透特性影响;推导了变渗透系数浑水渗流计算微分方程,通过对比验证和公式修正,结果表明:粗粒土中颗粒淤积率与控制粒径C_(0.075)含量符合Boltzmann函数关系;C_(0.075)是重要的影响因子,浑水颗粒在粗粒土柱的运移、沉积和淤堵形态过程大致可分为4种形态:表层堆积淤堵形态(S型);表层-内部双重淤堵形态(S-I型);内部孔隙淤堵形态(I型);暂态孔隙淤堵形态(P型)。土柱C_(u)越大,孔隙度越小,浑水颗粒在粗粒土中运移也越困难,土柱填充性就越差;水头加剧了颗粒在土柱内部运移的速度,但也加速了其在顶部的沉积并封堵;S型时土柱0~2cm渗透系数下降速率最大,封堵时总体渗透系数最小,2cm以下相对孔隙率及随深度逐渐减小;S-I和I型土柱下部渗透系数明显滞后于上部,相对孔隙率存在临界深度(约为4~6cm);P型时土柱整体相对孔隙率基本无变化。

关键词：颗粒渗流粗粒土浑水堵塞