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Journal of Integrative Agriculture 2015年第6期14卷 1171-1183页

作者： CHEN Hua-tao CHEN Xin WU Bing-yue yuan xing-xing ZHANG Hong-mei CUI Xiao-yan LIU Xiao-qing Institute of Vegetable Crops Jiangsu Academy of Agricultural Sciences

Sodium toxicity and potassium insufficient are important factors affecting the growth and development of soybean in saline soil. As the capacity of plants to maintain a high cytosolic, K^＋/Na^＋ ratio is the key determinant of tolerance under salt stress. The aims of the present study were to identify and analyse expression patterns of the soybean K^＋ efflux antiporter（KEA） gene and Na^＋/H^＋ antiporter（NHX） gene family, and to explore their roles under abiotic stress. As a result, 12 soybean Gm KEAs genes and 10 soybean Gm NHXs genes were identified and analyzed from soybean genome. Interestingly, the novel soybean KEA gene Glyma16g32821 which encodes 11 transmembrane domains were extremely up-regulated and remained high level until 48 h in root after the excessive potassium treatment and lack of potassium treatment, respectively. The novel soybean NHX gene Glyma09g02130 which encodes 10 transmembrane domains were extremely up-regulated and remained high level until 48 h in root with Na Cl stress. Imaging of subcellular locations of the two new Glyma16g32821-GFP and Glyma09g02130-GFP fusion proteins indicated all plasma membrane localizations of the two novel soybean genes. The 3D structures indicated that the two soybean novel proteins Glyma09g02130（NHX） and Glyma16g32821（KEA） all belong to the cation/hydrogen antiporter family.

关键词： soybean KEA NHX and Na antiporter abiotic potassium extremely transmembrane maintain

Advances in salinity tolerance of soybean: Genetic diversity, heredity, and gene identification contribute to improving salinity tolerance

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Journal of Integrative Agriculture 2018年第10期17卷 2215-2221页

作者： CHEN Hua-tao LIU Xiao-qing ZHANG Hong-mei yuan xing-xing GU He-ping CUI Xiao-yan CHEN Xin Institute of Industrial Crops Jiangsu Academy of Agricultural Sciences

Salt stress is one of the major abiotic stresses affecting soybean growth. Genetic improvement for salt tolerance is an effective way to protect soybean yield under salt stress conditions. Successful improvement of salt tolerance in soybean relies on identifying genetic variation that confers tolerance in soybean germplasm and subsequently incorporating these genetic resources into cultivars. In this review, we summarize the progress in genetic diversity and genetics of salt tolerance in soybean, which includes identifying genetic diversity for salt tolerant germplasm; mapping QTLs conferring salt tolerance; map-based cloning; and conducting genome-wide association study（GWAS） analysis in soybean. Future research avenues are also discussed, including high throughput phenotyping technology, the CRISPR/Cas9 Genome-Editing System, and genomic selection technology for molecular breeding of salt tolerance.

关键词： soybean genetic variation heredity gene identification salinity improvement

Construction of a high-density adzuki bean genetic map and evaluation of its utility based on a QTL analysis of seed size

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Journal of Integrative Agriculture 2021年第7期20卷 1753-1761页

作者： WANG Li-xia WANG Jie LUO Gao-ling yuan xing-xing GONG Dan HU Liang-liang WANG Su-hua CHEN Hong-lin CHEN Xin CHENG Xu-zhen nstitute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081P.R.China Institute of Rice Research Guangxi Academy of Agricultural SciencesNanning 530007P.R.China Institute of Economic Crops Jiangsu Academy of Agricultural SciencesNanjing 210014P.R.China

Adzuki bean(Vigna angularis(Willd.)Ohwi&Ohashi)is an annual cultivated leguminous crop commonly grown in Asia and consumed ***,there has been limited research regarding adzuki bean genetics,which has prevented the efficient application of genes during *** the present study,we constructed a high-density genetic map based on whole genome re-sequencing technology and validated its utility by mining QTLs related to seed ***,we analyzed the sequences flanking insertions/deletions(In Dels)to develop a set of PCR-based markers useful for characterizing adzuki bean genetics.A total of 2904 markers were mapped to 11 linkage groups(LGs).The total length of the map was 1365.0 cM,with an average distance between markers of 0.47 *** the LGs,the number of markers ranged from 208(LG7)to 397(LG1)and the total distance ranged from 97.4 cM(LG9)to 155.6 cM(LG1).Twelve QTLs related to seed size were identified using the constructed *** two major QTLs in LG2 and LG9 explained 22.1 and 18.8%of the total phenotypic variation,*** minor QTLs in LG4,LG5 and LG6 explained 3.0–10.4%of the total phenotypic variation.A total of 9718 primer pairs were designed based on the sequences flanking In *** the 200 selected primer pairs,75 revealed polymorphisms in 24 adzuki bean *** genetic map constructed in this study will be useful for screening genes related to other ***,the QTL analysis of seed size and the novel markers described herein may be relevant for future molecular investigations of adzuki bean and will be useful for exploiting the mechanisms underlying legume seed development.

关键词： Vigna angularis genetic map QTL seed size PCR-based marker development

Observation on effects of moxibustion at abdominal acupoints for slow transit constipation due to yang deficiency of the spleen and kidney

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Journal of Acupuncture and Tuina Science 2017年第6期15卷 426-433页

作者： Zhang Di yuan xing-xing Wang Bing-yu Zhang Ya-li[1[ Nangang Branch of Heilongjiang Provincial Academy of Chinese Medicine Harbin 150001 China Research Institute of Senile Constipation Heilongjiang Provincial Academy of Chinese Medicine Harbin 150001 China

Objective： To observe the clinical effects of moxibustion at abdominal acupoints for slow transit constipation（STC） due to yang deficiency of the spleen and kidney. Methods： A total of 52 cases with slow transit constipation in conformity with the inclusion criteria were selected and divided into a control group and an observation group according to their visit order and random digital table, 26 cases in each group. Patients in the control group received routine nursing guide. Besides the same routine nursing guide, patients in the observation group received moxibustion at the abdominal acupoints, once every day. The course of the treatment was 4 weeks in the two groups, and the 3-month follow-up was given after the course was finished, for comparing the clinical symptoms, results of colon transit tests, scores of depression/anxiety scale and nursing satisfaction. Results： The total effective rate was 92.3% in the observation group and 69.2% in the control group, with a significant difference between the two groups. After the treatment and during the follow-up checks, the scores of Chinese medical symptoms in the two groups were remarkably decreased than those before the treatment（all P〈0.01）; the scores of the observation group were obviously lower than those in the control group（all P〈0.01）. The discharge rates of the markers in the two groups were remarkably increased than those at the same time period before the treatment; moreover, the discharge rates of the markers at various time periods were remarkably better in the observation group than those in the control group（P〈0.01）. SDS and SAS scores were remarkably decreased after the treatment in the two groups（P〈0.01）. In comparison of SDS score between the two groups after the treatment, the difference was statistically significant（P〈0.01）. In comparison of SAS score between the two groups after the treatment, the difference was statistically significant（P〈0.05）. The nursing satisfaction was 96.2% in the observa

关键词： Moxibustion Therapy Points, Abdomen Anxiety Depression Spleen-kidney Yang Deficiency Constipation

基于显著性特征和角度信息的遥感图像目标检测

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计算机科学 2021年第4期48卷 174-179页

作者：袁星星吴秦江南大学人工智能与计算机学院江苏无锡214122 江南大学江苏省模式识别与计算智能工程实验室江苏无锡214122

遥感图像中的目标具有密集性、多尺度和多角度等特性,这使得遥感图像多类别目标检测成为一项具有挑战性的课题。因此,文中提出了一种新的端到端的遥感图像目标检测框架。该框架通过提取显著性特征和不同卷积通道之间的相互关系来增强目... 详细信息

遥感图像中的目标具有密集性、多尺度和多角度等特性,这使得遥感图像多类别目标检测成为一项具有挑战性的课题。因此,文中提出了一种新的端到端的遥感图像目标检测框架。该框架通过提取显著性特征和不同卷积通道之间的相互关系来增强目标信息,抑制非目标信息,从而提高特征的表示能力。同时,在不增加模型参数的情况下,在卷积模块中添加多尺度特征模块来捕获更多的上下文信息。为了解决遥感图像中目标角度多变这一问题,该框架在区域建议网络中加入了角度信息,得到有角度的矩形候选框,并在训练过程中添加注意力损失函数来引导网络学习显著性特征。该框架在公开的遥感图像数据集上进行了相关验证,在水平任务框和方向任务框上的实验结果证明了所提方法的有效性。

关键词：遥感图像目标检测多尺度特征通道自学习显著性学习

Insight Into the Separation-of-Variable Methods for the Closed-Form Solutions of Free Vibration of Rectangular Thin Plates

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Computer Modeling in Engineering & Sciences 2025年第1期142卷 329-355页

作者： Yufeng xing Ye yuan Gen Li Institute of Solid Mechanics Beihang University(BUAA)Beijing100191China

The separation-of-variable(SOV)methods,such as the improved SOV method,the variational SOV method,and the extended SOV method,have been proposed by the present authors and coworkers to obtain the closed-form analytical solutions for free vibration and eigenbuckling of rectangular plates and circular cylindrical *** taking the free vibration of rectangular thin plates as an example,this work presents the theoretical framework of the SOV methods in an instructive way,and the bisection–based solution procedures for a group of nonlinear eigenvalue ***,the explicit equations of nodal lines of the SOV methods are presented,and the relations of nodal line patterns and frequency orders are *** is concluded that the highly accurate SOV methods have the same accuracy for all frequencies,the mode shapes about repeated frequencies can also be precisely captured,and the SOV methods do not have the problem of missing roots as well.

关键词： Separation-of-variable method Rayleigh quotient nodal line eigenvalue equation bisection method

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某地铁车站地下连续墙特殊地段导墙的施工

山西建筑 2009年第7期35卷 169-170页

作者：袁星星中铁隧道集团北京中铁隧建筑有限公司

针对某地铁车站地下连续墙导墙的位置与一市政废弃雨水箱涵在平面位置上发生冲突的问题,提出了对雨水箱涵顶板以上路面进行破除,土体开挖支护后部分破除雨水箱涵后,在雨水箱涵底部开始施作地下连续墙导墙的方法,介绍了具体的施工工艺和... 详细信息

针对某地铁车站地下连续墙导墙的位置与一市政废弃雨水箱涵在平面位置上发生冲突的问题,提出了对雨水箱涵顶板以上路面进行破除,土体开挖支护后部分破除雨水箱涵后,在雨水箱涵底部开始施作地下连续墙导墙的方法,介绍了具体的施工工艺和技术要求,从而安全、有效地保证地下连续墙在此特殊地段的正常施工。

关键词：导墙废弃雨水箱涵施工方法技术要求

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地区收入差距、人口流动与刑事犯罪率

南方人口 2023年第1期38卷 46-58页

作者：洪勇袁兴兴九江学院江西开放型经济研究中心江西九江332005 九江学院经济学院江西九江332005 河南日报社经营管理部河南郑州450008

本文将地区收入差距、人口流动分为省内与省际收入差距、省内与省际人口流动,分析了它们对省级层面刑事犯罪率的影响。结果表明:(1)随着省内和省际收入差距扩大、省内和省际人口流动规模扩张,刑事犯罪率会显著上升,且省内收入差距和人... 详细信息

本文将地区收入差距、人口流动分为省内与省际收入差距、省内与省际人口流动,分析了它们对省级层面刑事犯罪率的影响。结果表明:(1)随着省内和省际收入差距扩大、省内和省际人口流动规模扩张,刑事犯罪率会显著上升,且省内收入差距和人口流动对刑事犯罪率的影响分别比省际收入差距和人口流动大。(2)失业率、离婚率、城镇化水平、人口性别比、房价的提高会显著推升刑事犯罪率,受教育水平和社会保障水平的提高可以显著降低刑事犯罪率,司法支出和人口密度对刑事犯罪率没有实质性影响。(3)人口流动对刑事犯罪率的作用机制会受到地区收入差距的影响。最后,本文提出了一系列降低刑事犯罪率的政策建议。

关键词：刑事犯罪率地区收入差距人口流动门限面板模型

平喘颗粒通过lncRNA MALAT1/miR-140-5p/VEGFA抑制哮喘气道血管新生的作用机制

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中华中医药杂志 2021年第4期36卷 2006-2012页

作者：袁星星王雪慧王瑶李竹英黑龙江中医药大学哈尔滨150040 黑龙江省中医药科学院南岗分院哈尔滨150006 黑龙江中医药大学附属第一医院哈尔滨150040

目的:挖掘哮喘的潜在ceRNA网络并探索平喘颗粒抑制哮喘气道血管新生的机制。方法:利用GSE117038数据集,分析文件中差异表达的lncRNA和mRNA,通过比对、预测构建lncRNA-miRNA-mRNA关联,构建ceRNA调控网络。通过屋尘螨(HDM)诱导构建哮喘小... 详细信息

目的:挖掘哮喘的潜在ceRNA网络并探索平喘颗粒抑制哮喘气道血管新生的机制。方法:利用GSE117038数据集,分析文件中差异表达的lncRNA和mRNA,通过比对、预测构建lncRNA-miRNA-mRNA关联,构建ceRNA调控网络。通过屋尘螨(HDM)诱导构建哮喘小鼠模型,并给予平喘颗粒灌胃治疗。分别采用HE染色、Masson染色和免疫组化法检测肺组织病理改变和VEGFA蛋白的表达,采用qRT-PCR法检测肺组织中ceRNA调控网络中关键基因的表达。体外实验观察平喘颗粒含药血清对脐静脉内皮细胞形态、增殖、迁移和ceRNA调控网络中关键基因表达的影响。结果:差异分析共得到816个差异lncRNA,其中下调86个,上调730个,3 171个差异mRNA,其中下调354个,上调2 817个。通过预测lncRNA结合的miRNA共206个,miRNA靶向调控的mRNA共1 269,取交集后得到300个差异mRNA,并构建ceRNA网络。体内实验表明,与模型组比较,平喘颗粒能够显著抑制哮喘气道炎症、胶原沉积和VEGFA蛋白的表达,同时降低肺组织中lncRNA MALAT1和VEGFA mRNA的表达(P<0.05)。体外实验表明,过表达lncRNA MALAT1后HUVEC细胞增殖和迁移数量显著增加(P<0.05)。与MALAT1过表达组比较,平喘颗粒组能够显著抑制HUVEC细胞增殖和迁移,抑制HUVEC细胞lncRNA MALAT1和VEGFA mRNA的表达,增加miR-140-5p的表达(P<0.05)。结论:平喘颗粒能够下调肺组织中lncRNA MALAT1的表达,通过内源性竞争机制进而增加miR-140-5p与VEGFA的结合,从而促进其降解,达到抑制血管新生的作用。

关键词：哮喘气道重塑血管新生 ceRNA网络内皮细胞平喘颗粒

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地下过街通道管井降水施工实例分析

铁道科学与工程学报 2006年第2期3卷 70-73页

作者：王立川袁星星赵岗领中铁隧道集团有限公司北京分公司北京100022

通过水文地质调查和现场降水试验,提出了适用于无隔水层而引起滞水存赋的强透水地层中开挖浅埋地下结构的“大管井群降,小管井辅助”的降水方案。分析了该方案在沈阳市文安路地下过街通道施工中的应用过程及其降水效果。研究结果表明,... 详细信息

通过水文地质调查和现场降水试验,提出了适用于无隔水层而引起滞水存赋的强透水地层中开挖浅埋地下结构的“大管井群降,小管井辅助”的降水方案。分析了该方案在沈阳市文安路地下过街通道施工中的应用过程及其降水效果。研究结果表明,对于渗水系数较高的强透水圆砾地层,采用该方案可有效地解决高水位的问题,满足通道开挖支护无水作业的要求;除局部地表出现较大的沉降变形外,对周围建筑物未造成明显的影响。该方案可对类似浅埋地下结构的降水施工提供参考。

关键词：地下工程过街通道浅埋管井降水