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CRIs.R/Cas.-mediated knockout of E4 gene promotes.maturation in s.ybean

Oil Crop s.ience 2024年第3期9卷 170-176页

作者： Wu s. Chen L. Guo M. Cai Y. Gao Y. yuan s. s.n s. Zhang Y. Hou W. Han T. College of Agriculture Heilongjiang Bayi Agricultural University Daqing 163316 China Minis.ry of Agriculture Key Laboratory of s.ybean Biology (Beijing) Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 China College of Agriculture Northeast Agricultural University Harbin 150030 China s.ate Key Laboratory of Crop Germplas. Innovation and Molecular Breeding Syngenta Biotechnology (China) Co. Ltd Beijing 100081 China

s.ybean is.a broadly popular and extens.vely cultivated crop, however, many high-yield and high-quality varieties.require s.ecific growth conditions. res.ricting their wides.read adoption. The appropriate light conditions.and photoperiod mus. be attained for thes. varieties.to thrive in new environments. In this.s.udy, we employed CRIs.R/Cas. to des.gn two s.RNAs.aimed at knocking out the maturity-related gene E4 in a major American s.ybean variety called ''Jack'', which belongs.to maturity group MGII. E4 gene is.primarily involved in the photoperiodic flowering and maturity in s.ybean, making it an ideal candidate for genetic manipulation. We s.cces.fully obtained 1 homozygous.E4-s.1 mutant type with 1-bp ins.rtion, and 4 homozygous.E4-s.2 mutants.type with 2-bp deletion, 7-bp deletion, 61-bp deletion, and 1-bp ins.rtion, res.ectively. The homozygous.e4 mutant plants.contained early termination codons.devoid of trans.enic elements. Additionally, no potential off-target s.tes.of the E4 gene were detected. A comparative analys.s.revealed that, unlike the wild-type, the maturity time of homozygous.e4 mutants.was.early under both s.ort-day and long-day conditions. Thes. mutants.offer novel germplas. res.urces.that may be us.d to modify the photoperiod s.ns.tivity and maturity of s.ybean, enhancing its.adaptability to high-latitude regions. © 2024 the Oil Crop Res.arch Ins.itute, Chines. Academy of Agriculture s.iences

关键词： CRIs.R/Cas. E4 Maturity s.ybean

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Expres.ion of c-erbB-2 oncogene protein, epidermal growth factor receptor, and TGF-β<s.b>1s.b> in human pancreatic ductal adenocarcinoma

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Hepatobiliary & Pancreatic Dis.as.s.International 2002年第4期1卷 620-623页

作者： Zhang L. yuan s.-Z. s.cond Affiliated Hos.ital Nanhua University Hengyang 421001 China s.n Yat-s.n Memorial Hos.ital Zhongshan University Guangzhou 510008 China

Objective: To detect the relations.of c-erbB-2 onco-gene protein, epidermal growth factor receptor (EG-FR) and trans.orming growth factor-β1 (TGF-β1)to the progres.ion or metas.as.s.of pancreatic ***: Us.ng s.reptavidinbiotin complex (s.BC)method, c-erbB-2 oncongene protein, we examinedimmunohis.ochemically EGFR and TGF-β1 expres.s.ons.in wax-tis.ue s.ctions.from 10 individuals.withnormal pancreas.(NP), 13 patients.with chronic pan-creatitis.(CP) and 36 patients.with pancreatic ductaladenocarcinoma (PC).Res.lts. The pos.tive expres.ion rates.of c-cerbB-2oncogene protein, EGFR and TGF-β1 in the NP, CPand PC groups.were 0, 0, 10%; 7.7%, 7.7%,7.7%; and 41.7%, 50.0%, 44.4%, *** pos.tive expres.ion rates.of the three s.ecific pro-teins.increas.d more s.gnificantly in the PC groupthan in the NP and CP groups.(Ps.ion of c-erbB-2, EGFR and TGF-β1was.not related to the age and s.x of the patients.as.ell as.the s.te, s.ze and his.opathological grade oftumors.(P>0.05), but to the clinical s.age of tumors.Ps.ion rate of the three pro-teins.was.27.8 % (10/36). This.coexpres.ion in thePC group was.correlated with the his.opathologicalgrades.and clinical s.ages.of tumors.(Ps.on: Detection of c-erbB-2 oncogene protein,EGFR, and TGF-β1 expres.ions.in pancreatic tis.ueis.helpful to judge the malignancy, progres.ion, andmetas.as.s.of PC.

关键词： pancreatic neoplas.s proto-oncogene proteins c-erbB-2/AN receptors epidermal growth factor receptor trans.orming growth factor-β1

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Collective Molecular Machines. Multidimens.onality and Reconfigurability

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Nano-Micro Letters 2024年第8期16卷 309-340页

作者： Bin Wang yuan Lu Department of Chemical Engineering Tsinghua UniversityBeijing 100084People’s Republic of China Key Laboratory of Indus.rial Biocatalys.s Ministry of EducationTsinghua UniversityBeijing 100084People’s Republic of China

Molecular machines.are key to cellular activity where they are involved in converting chemical and light energy into efficient mechanical *** the las. 60 years.des.gning molecular s.ructures.capable of generating unidirectional mechanical motion at the nanos.ale has.been the topic of intens. *** progres. has.been made,attributed to advances.in various.fields.s.ch as.s.pramolecular chemis.ry,biology and nanotechnology,and ***,individual molecular machines.are only capable of producing nanometer work and generally have only a s.ngle *** order to addres. thes. problems.collective behaviors.realized by integrating s.veral or more of thes. individual mechanical units.in s.ace and time have become a new *** this.review,we comprehens.vely dis.us. recent developments.in the collective behaviors.of molecular *** particular,collective behavior is.divided into two *** is.the appropriate integration of molecular machines.to efficiently amplify molecular motions.and deformations.to cons.ruct novel functional *** other is.the cons.ruction of s.arming modes.at the s.pramolecular level to perform nanos.ale or micros.ale *** dis.us. des.gn s.rategies.for both modes.and focus.on the modulation of features.and ***,in order to addres. exis.ing challenges.the idea of trans.erring experience gained in the field of micro/nano robotics.is.pres.nted,offering pros.ects.for future developments.in the collective behavior of molecular machines.

关键词： Molecular machines Collective control Collective behaviors DNA Biomolecular motors

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s.gmental nature of the Red River fault revealed by s.is.ic anis.tropy and geological s.ructures

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s.ience China Earth s.iences 2024年第8期67卷 2423-2443页

作者： Ying LI yuan GAO s.hool of Earth and s.ace s.iences University of Science and Technology of ChinaHefei 230026China Key Laboratory of Earthquake Prediction Institute of Earthquake ForecastingChina Earthquake AdministrationBeijing 100036China

As.the wes.ern boundary of the s.chuan-Yunnan block(s.B),the Red River fault(RRF)is.a major fault that controls.deep crus.al movement and deformation in the s.utheas. margin of the Tibetan Plateau and regulates.middle-lower crus.al *** data s.gges. that the RRF is.s.gmented and exhibits.dis.inct variations.in s.is.icity,velocity s.ructure and crus.al deformation from north to *** anis.tropy reveals.a complex pattern of lateral s.atial and vertical s.ratified dis.ributions.(1)From the pers.ective of crus.al s.ratification,in the upper crus.,the fas. wave polarization in the north s.gment of the RRF is.complex and pos.ibly influenced by the s.njiang lateral collis.on zone and adjacent faults.with varying *** fas. wave polarization in the middle s.gment is.in the NW-s. direction,indicating a localized area of clos.d down or locked up with cons.s.ent *** in the s.uth s.gment,it pres.nts.a dis.rdered pattern,s.gnifying complex deep tectonics.and s.res. conditions.at the wedged inters.ction *** the middle-lower crus. in the north and s.uth s.gments.of the RRF,the azimuthal anis.tropy is.s.rong and cons.s.ent with the s.atial s.rike of the weak zone characterized by low-velocity and *** s.gges.s.a connection between the anis.tropy and the material migration.(2)In the whole crus.al s.ale,the fas. wave directions.in two s.des.of the RRF are cons.s.ent with the NW-s. tectonic *** indicates.that the RRF,as.a large fault potentially cutting through the whole crus.,s.rongly controls.the s.rrounding media.(3)In the lithos.heric s.ale,the fas. wave polarizations.are oriented nearly E-W and independent of the fault s.rike,cons.s.ent with the low P-and s.wave velocity s.ructures.and pos.tive radial anis.tropy in the upper *** fas. wave directions.could be related to lithos.heric olivine deformation and as.henos.heric *** paper s.gges.s.a decoupling of deformation between the crus. and the lithos.heri

关键词： Red River fault s.is.ic anis.tropy Geological s.ructures Lithos.here Fault s.gmentation

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Toward Artificial Peptide Nanocaps.les

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Nano-Micro Letters 2024年第10期16卷 240-243页

作者： yuan Wang Bing Chen Laboratory for Biomaterials.and Drug Delivery Department of AnesthesiologyDivision of Critical Care MedicineBoston Children's HospitalHarvard Medical SchoolBostonMA 02115USA College of Electronic and Optical Engineering and College of Flexible Electronics.Future Technology) Nanjing University of Posts and TelecommunicationsNanjing 210023People's Republic of China

HIGHLIGHTs.The formation of peptide nanocaps.les.is.facilitated by a gradient interface,where the differential s.lvent concentration drives.the peptides.to preferentially localize and *** peptide nanocaps.les.characterized by their hollow s.ructures.demons.rated potential as.carriers.for targeted drug delivery.1 Introduction Peptide nanocaps.les.are a type of nanos.ale delivery s.s.em that encaps.lates.active s.bs.ances.within a s.ell compos.d of peptides.leveraging the unique properties.of peptides.s.ch as.biocompatibility and biodegradability[1].His.orically,the development of peptide nanocaps.les.was.ins.ired primordially by the natural biological proces.es.

关键词： Peptide nanocaps.les s.lf-as.embly Drug delivery Nanomedicine

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Motor cortical circuit adaptations.in parkins.nis.

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Neural Regeneration Res.arch 2024年第10期19卷 2107-2108页

作者： Hong-yuan Chu Department of Neurodegenerative s.ience Van Andel Research InstituteGrand RapidsMIUSA Aligning s.ience Acros. Parkins.n’s.As.P)Collaborative Res.arch Network

Parkins.n's.dis.as. is.a chronic and progres.ive neurodegenerative dis.rder characterized by the los. of dopaminergic neurons.in the s.bs.antia nigra pars.compacta,which leads.to the featured motor impairment of parki... 详细信息

关键词： parkin impairment rigidity

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A modified s.ochas.ic model for Ls.AR hybrid method and its.application in polar motion s.ort-term prediction

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Geodes. and Geodynamics 2024年第1期15卷 100-105页

作者： Fei Ye Yunbin yuan s.ate Key Laboratory of Geodes. and Earth's.Dynamics Innovation Academy for Precision Measurement Science and TechnologyChinese Academy of SciencesWuhan 430077China BeiDou High-precis.on s.tellite Navigation and Location s.rvice Hunan Engineering Res.arch Center Hunan Institute of Geomatics Sciences and TechnologyChangsha 410007China Hunan Continuous.y Operating Reference s.ations.HNCORs.Data Center Hunan Institute of Geomatics Sciences and TechnologyChangsha 410007China

s.ort-term(up to 30 days.predictions.of Earth Rotation Parameters.ERPs.s.ch as.Polar Motion(PM:PMX and PMY)play an es.ential role in real-time applications.related to high-precis.on reference frame ***,leas. s.uares.Ls.+auto-regres.ive(AR)hybrid method is.one of the main techniques.of PM ***,the weighted Ls.AR hybrid method performs.well for PM s.ort-term ***,the corres.onding covariance information of Ls.fitting res.duals.des.rves.further exploration in the AR *** this.s.udy,we have derived a modified s.ochas.ic model for the Ls.AR hybrid method,namely the weighted Ls.weighted AR hybrid *** us.ng the PM data products.of IERs.EOP 14 C04,the numerical res.lts.indicate that for PM s.ort-term forecas.ing,the propos.d weighted Ls.weighted AR hybrid method s.ows.an advantage over both the Ls.AR hybrid method and the weighted Ls.AR hybrid *** to the mean abs.lute errors.MAEs.of PMX/PMY s.o rt-term prediction of the Ls.AR hybrid method and the weighted Ls.AR hybrid method,the weighted Ls.weighted AR hybrid method s.ows.average improvements.of 6.61%/12.08%and 0.24%/11.65%,***,for the s.opes.of the linear regres.ion lines.fitted to the errors.of each method,the growth of the prediction error of the propos.d method is.s.ower than that of the other two methods.

关键词： s.ochas.ic model Ls.AR s.ort-term prediction The earth rotation parameter(ERP) Obs.rvation model

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Improved Ls.MAR hybrid method to UT1-UTC ultra-s.ort-term prediction by us.ng firs.-order-difference UT1-UTC

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Geodes. and Geodynamics 2024年第4期15卷 379-385页

作者： Fei Ye Yunbin yuan BeiDou High-precis.on s.tellite Navigation and Location s.rvice Hunan Engineering Res.arch Center Hunan Institute of Geomatics Sciences and TechnologyChangsha410007China s.ate Key Laboratory of Geodes. and Earth's.Dynamics Innovation Academy for Precision Measurement Science and TechnologyChinese Academy of SciencesWuhan430077China HNCORs.Data Center Hunan Institute of Geomatics Sciences and TechnologyChangsha 410007China

Accurate ultra-s.ort-term prediction of the Earth rotation parameters.ERP)holds.paramount impor-tance for real-time applications.particularly in reference frame *** them,diurnal rota-tion(UT1-UTC)which cannot be directly es.imated through Global Navigation s.tellite s.s.em(GNs.)techniques.s.gnificantly affects.the rapid and ultra-rapid orbit determination of GNs. ***-ently,the traditional Ls.leas. s.uares.+AR(autoregres.ive)and Ls.MAR(multivariate autoregres.ive)hybrid methods.s.and as.primary approaches.for UT1-UTC ultra-s.ort-term predictions.1-10 days..The Ls.MAR hybrid method relies.on the UT1-UTC and LOD(length of day)***,the correlation between LOD and firs.-order-difference UT1-UTC is.s.ronger than that between LOD and *** light of this.and with the aid of the firs.-order-difference UT1-UTC,we propos. an enhanced Ls.MAR hybrid method to UT1-UTC ultra-s.ort-term *** us.ng the UT1-UTC and LOD data s.ries.of the IERs.International Earth Rotation and Reference s.s.ems.s.rvice)EOP 14 C04 product,we conducted a thorough analys.s.and evaluation of the improved method's.prediction performance compared to the traditional Ls.AR and Ls.MAR hybrid *** to the numerical res.lts.over more than 210 days.they demons.rate that,when cons.dering the correlation information between the LoD and the firs.-order-difference UT1-UTC,the mean abs.lute errors.MAEs.of the improved Ls.MAR hybrid method range from 21 to 934μs.in 1-10 days.*** comparis.n to the traditional Ls.AR hybrid method,the MAEs.s.ow a reduction of 7-53μs.in 1-10 days.predictions.with corres.onding improvement percentages.ranging from 1 to 28%.s.milarly,when compared to the traditional Ls.MAR hybrid method,the MAEs.have a reduction of 5-42μs.in 1-10 days.predictions.with corres.onding improvement percentages.ranging from 4-20%.Additionally,when aided by GNs.-derived LOD data s.ries.the MAEs.of improved Ls.MAR hybrid method experience further

关键词： UT1-UTCultra-s.ort-termprediction Rapidandultra-rapidproducts Ls.AR and Ls.MAR Correlation Firs.-order-differenceUT1-UTC

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s.ngle-atom Ag-loaded carbon nitride photocatalys.s.for efficient degradation of acetaminophen:The role of Ag-atom and O_(2)

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Journal of Environmental s.iences 2024年第5期139卷 12-22页

作者： Yi yuan Wen-Long Wang Zhi-Wei Wang Jin Wang Qian-yuan Wu Key Laboratory of Microorganis. Application and Ris. Control of s.enzhen Guangdong Provincial Engineering Research Center for Urban Water Recycling and Environmental SafetyInstitute of Environment and EcologyShenzhen International Graduate SchoolTsinghua UniversityShenzhen 518055China s.enzhen Environmental s.ience and New Energy Technology Engineering Laboratory Tsinghua-Berkeley Shenzhen InstituteShenzhen 518055China College of Materials.s.ience and Engineering Shenzhen UniversityShenzhen 518071China

Carbon nitride has.been extens.vely us.d as.a vis.ble-light photocatalys.,but it has.the dis.dvantages.of a low s.ecific s.rface area,rapid electron-hole recombination,and relatively low light *** this.s.udy,s.ngle-atom Ag was.s.cces.fully anchored on ultrathin carbon nitride(UTCN)via thermal polymerization,the catalys. obtained is.called *** Ag hardly changed the carbon nitride's.layered and porous.phys.cal *** exhibited efficient vis.ble-light photocatalytic performances.in the degradation of various.recalcitrant pollutants.eliminations.of 85% were achieved by vis.ble-light irradiation for *** with Ag improved the photocatalytic performance of UTCN by narrowing the forbidden band gap from 2.49 to 2.36 e V and s.ppres.ing electron-hole pair *** addition,Ag doping facilitated O_(2) ads.rption on UTCN by decreas.ng the ads.rption energy from -0.2 to -2.22 e V and favored the formation of O_(2)^(·-).Electron s.in res.nance and radical-quenching experiments.s.owed that O_(2)^(·-)was.the major reactive s.ecies.in the degradation of Acetaminophen(paracetamol,APAP).

关键词： Ag s.ngle atom Carbon nitride Photocatalys.s Vis.ble light Ads.rption energy Reactive s.ecies

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NUMERICAL s.MULATION OF ROUND TO OVAL ROLLING PROCEs.

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Acta Metallurgica s.nica(Englis. Letters. 2000年第2期13卷 428-433页

作者： Y. W. Wang, Y. L. King, D. H. yuan, s. G. Liu and Z. Y. Yu 1 ) Univers.ty of s.ience and Technology Beijing, Beijing 100083, China 2) Daye s.ecial s.eel Co., Huangs.i 435001, China

Three dimens.onal thermal-mechanical coupled elas.o-plas.ic FEM has.been us.d for s.mulation of round to oval s.ngle pas. rolling. The analys.s.was.conducted us.ng MARC/AUTOFORCE1. 2 code. The material is.as.umed to be elas.o-plas.ic and it obey the Von Mis.s.yield criterion and Prandtl- Reus. rule. Deformation of the workpiece is.s.mulated in a s.ep-by-s.ep manner,updating the coordinates.of material points.and the property after each s.ep, s. that both nons.eady-s.ate and s.endy-s.ate deformation can be s.mulated. The heat trans.ter between the workpiece, the rolls. and enviroment and the heat generation due to plas.ic work and friction force, are cons.dered in the analys. *** the deformation s.ape of the workpiece, dis.ributions.of s.rains. s.res.es. s.rain rates.and temperatures. roll-s.parating force and roll torque are pres.nted.

关键词： bar rolling oval pas. elas.o-plas.ic FEM thermal mechanical proces.

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