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Immunocytochemical Identification and Localization of Diffuse Neuroendocrine System (DNES) Cells in Gastrointestinal Tract of Channel Catfish (Ictalurus punctatus)

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Agricultural Sciences in China 2009年第2期8卷 238-243页

作者： HE Min WANG Kai-yu zhang yu College of Veterinary Medicine Sichuan Agricultural University Ya＇an 625014 P.R.China

To detect distribution and relative frequency of diffuse neuroendocrine system （DNES） cells in the gastrointestinal tract of channel catfish （lctalurus punctatus）, the intestinal tract of channel catfish was divided into seven portions from proximal to distal： the enlarged area after oesophagus, cardia, fundus, pylorus, and anterior, middle, and posterior intestine. Immunohistochemical method using the strept avidin-biotin-complex （SABC） was employed. All antisera between seven portions of the channel catfish were compared statistically using statistical package for the social science （SPSS）. Five types of DNES ceils were determined： neuropeptide Y-immunoreactive （NPY-IR） cells were demonstrated in both anterior and middle intestine; serotonin （5-HT） immunoreactive cells were detected throughout the whole gastrointestinal tract; vasoactive intestinal peptide （VIP） positive cells were at the highest frequency in pylorus; glucagon-immunoreactive （GLU-IR） cells were moderate in number in the fundus and anterior, middle intestine, and no immunoreactivity was determined in the other portions; somatostatin （SOM） positive cells were more abundant in the anterior and middle intestine. The regional distribution and relative frequency of immunoreactive cells in the channel catfish, Ictalurus punctatus, are essentially similar to those of other fish. However, some characteristics are observed in this species, which further proved that the diversity of the physiological function of DNES cells was based on their morphology.

关键词： channel catfish lctalurus punctatus gastrointestinal tract DNES cells immunocytochemistry

In situ Remediation of Petroleum Contaminated Groundwater by Permeable Reactive Barrier with Hydrothermal Palygorskite as Medium

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Chemical Research in Chinese Universities 2013年第1期29卷 37-41页

作者： zhang Sheng-yu zhang yu-ling SU Xiao-si zhang Ying Institute of Water Resources and Environmental Key Lab of Groundwater Resources and Environment Ministry of Education Jilin University Changchun 130026 P. R. China

The permeable reactive barrier（PRB） has proven to be a costeffective technique to remediate the petro leum contaminated groundwater at a northeast field site in China. In this study, the geology, hydrogeology and con tamination characterization of the field site were investigated and the natural hydrothermal palygorskite was chosen as a reactive medium. Furthermore, the adsorption of the total petroleum hydrocarbons（TPH） in the groundwater onto hydrothermal palygorskite and the adsorption kinetics were investigated. The results indicate that the removal rates of TPH, benzene, naphthalene and phenantharene could all reach up to 90% by hydrothermal palygorskite with a diameter of 0.25-2.00 mm that had been thermally pretreated at 140 ℃. The adsorption of TPH onto hydrothermal palygorskite after pretreatment followed a pseudosecondorder kinetic model and a Langmuir adsorption isotherm, suggesting that the theoretic adsorption capacity of hydrothermal palygorskite for adsorbate could be 4.2 g/g. Scan ning electron microscopy（SEM）, infrared spectroscopy（IR）, Xray diffraction（XRD） and Xray fluorescence spec troscopy（XRF） were carried out to analyze the adsorption mechanism. The results reveal that hydrothermal palygors kite is a fibrous silicate mineral enriched in Mg and A1 with large surface area and porosity. The dense cluster acicular and fibrous crystal of hydrothermal palygorskite, and its effect polar group OH played an important role in the physical and chemical adsorption processes of it for contaminants. This study has demonstrated hydrothermal paly gorskite is a reliable reactive medium for in situ remediation of petroleum contaminated groundwater at field sites.

关键词： Petroleum contaminated groundwater Hydrothermal palygorskite Total petroleum hydrocarbon(TPH) Adsorption

40-Gb/s all-optical digital 4-bit priority encoder employing cross-gain modulation in semiconductor optical amplifiers

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Chinese Science Bulletin 2012年第10期57卷 1204-1208页

作者： LEI Lei DONG JianJi zhang Yin yu yu zhang XinLiang Wuhan National Laboratory for Optoelectronics and the School of Optoelectronic Science and Engineering Huazhong University of Science andTechnology Wuhan 430074 China

High-speed all-optical logic circuits have attracted much attention because of their important roles in signal processing in next-generation optical *** digital encoder is widely used in binary calculation,multiplexing,demultiplexing,address recognition and data encryption.A priority encoder allows the existence of multiple valid inputs simultaneously,identifies the priority of the request signals and encodes the *** propose and experimentally demonstrate an all-optical 4-bit priority encoder for return-to-zero signals at 40 Gbit/s based on cross-gain modulation in semiconductor optical *** fil-ters after semiconductor optical amplifiers are employed to improve the output *** logic bit sequences and clear open eye patterns with extinction ratios exceeding 10 dB are achieved.

关键词：半导体光放大器优先编码器交叉增益调制全光 Gb 信号处理下一代光网络逻辑电路

Expression of kallikrein-related peptidase 7 is decreased in prostate cancer

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Asian Journal of Andrology 2015年第1期17卷 106-110,I0010页

作者： Chong-yu zhang yu Zhu Wen-Bin Rui Jun Dai Zhou-Jun Shen Department of Urology Ruijin Hospital School of Medicine Shanghai Jiaotong University Shanghai China.

Recent evidence suggests that the human kallikrein 7 （KLK7） is differentially regulated in a variety of tumors. The aim of this study was to determine the expression of kallikrein-related peptidase 7 and KLK7 in our large collection of prostate samples. Between August 2000 and December 2012, 116 patients with histologically confirmed prostate cancer （PCa） and 92 with benign prostate hyperplasia （BPH） were recruited into the study. Using immunohistochemistry, quantitative reverse transcription polymerase chain reaction （RT-PCR） and western blot, kallikrein-related peptidase 7 expression in BPH and PCa tissues was determined at the mRNA and protein levels. The relationships between kallikrein-related peptidase 7 mRNA expression and clinicopathological features were analyzed. A total of 64 of 92 （69.57%） benign cases showed positive staining for KLK7 and 23 of 116 （19.83%） malignant cases showed positive, the difference of KLK7 expression between PCa and BPH was statistically significant （P〈 0.001）. The expression level of kallikrein-related peptidase 7 mRNA was significantly decreased in PCa tissues compared with that in BPH tissues and normal prostate tissue. Kallikrein-related peptidase 7 mRNA exhibited different expression patterns in terms of localization depending on pathological category of PCa. Similarly, our western immunoblot analyses demonstrated that the protein expression levels of KLK7 was lower in PCa than in BPH tissues and normal prostate tissue. Kallikrein-related peptidase 7 and KLK7 expression are down-regulated in PCa and lower expression of kallikrein-related peptidase 7 closely correlates with higher Gleason score and higher prostate-specific antigen level.

关键词： human kallikrein 7 kallikrein-related peptidase 7 prostate cancer prostate-specific antigen

A Rice Panicle Mutant Created by Transformation with an Antisense cDNA Library

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Journal of Integrative Plant Biology 2006年第11期48卷 1300-1305页

作者： yuan-Ling Chen Qun-yu zhang yu-yu Jian yue-Sheng Yang Kai-Dong Liu Yao-Guang Liu Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong College of Life Sciences South China Agricultural University Guangzhou 510642 China

A rice （Oryza sativa L.） mutant displaying defects in panicle development was identified among transformants in a transgenic mutagenlzed experiment using an antlsense cDNA library prepared from young rice panicles. In the mutant, the average splkelet number was reduced to 59.8 compared with 104.3 in wild-type plants. In addition, the seed-setting rate of the mutant was low （39.3%） owing to abnormal female development. Genetic analysis of T1 and T2 progeny showed that the traits segregated In a 3 （mutant）： 1 （wild type） ratio and the mutation was cosegregated with the transgene. Southern blot and thermal asymmetric interlaced polymerase chain reaction analyses showed that the mutant had a single T-DNA insertion on chromosome 5, where no gene was tagged. Sequencing analysis found that the transgenic antisense cDNA was derived from a gene encoding an F-box protein in chromosome 7 with unidentified function. This and another four homologous genes encoding putative F-box proteins form a gene cluster. These results indicate that the phenotyplc mutations were most likely due to the silencing effect of the expressed transgenic antisense construct on the member（s） of the F-box gene cluster.

关键词： antisense gene silencing F-box protein panicle development rice.

Topological Stability and Entropy for Certain Set-valued Maps

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Acta Mathematica Sinica,English Series 2024年第4期40卷 962-984页

作者： yu zhang yu Jun ZHU School of Mathematical Sciences Xiamen UniversityXiamen 361005P.R.China

In this paper,the dynamics(including shadowing property,expansiveness,topological stability and entropy)of several types of upper semi-continuous set-valued maps are mainly considered from differentiable dynamical systems points of *** is shown that(1)if f is a hyperbolic endomorphism then for eachε>0 there exists a C^(1)-neighborhood U of f such that the induced set-valued map F_(f,U)has theε-shadowing property,and moreover,if f is an expanding endomorphism then there exists a C^(1)-neighborhood U of f such that the induced set-valued map F_(f,U)has the Lipschitz shadowing property;(2)when a set-valued map F is generated by finite expanding endomorphisms,it has the shadowing property,and moreover,if the collection of the generators has no coincidence point then F is expansive and hence is topologically stable;(3)if f is an expanding endomorphism then for eachε>0 there exists a C^(1)-neighborhood U of f such that h(F_(f,U,ε))=h(f);(4)when F is generated by finite expanding endomorphisms with no coincidence point,the entropy formula of F is ***,the dynamics of the set-valued maps based on discontinuous maps on the interval are also considered.

关键词： Set-valued map orbit space hyperbolic endomorphism perturbation shadowing expansiveness topological stability entropy

Microscopic ensemble bootstrap in phase space

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Communications in Theoretical Physics 2024年第6期76卷 72-76页

作者： yu zhang College of Mechanics and Engineering Science Hohai UniversityNanjing 211100China

The bootstrap method which has been studied under many quantum mechanical models turns out to be feasible in microcanonical ensembles as *** the approach of Nakayama(2022 ***.A 372250054)produces a sector when energy is negative,in this paper we report a method that has stronger constraints and results in a smaller *** also study other models to demonstrate the effectiveness of our method.

关键词： numerical bootstrap microcanonical ensemble Poisson bracket bootstrap oscillator

Characteristics of a pre-combustion plasma jet igniter

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Chinese Journal of Aeronautics 2024年第7期37卷 178-189页

作者： Jinlu yu Baowen zhang Yang yu Bingbing ZHAO Lei zhang Aviation Engineering School Air Force Engineering UniversityXi’an 710038China Equipment Management and Unmanned Aerial Vehicle Engineering School Air Force Engineering UniversityXi’an 710038China

Plasma ignition technology has delivered good performance in the aerospace industry. In this study, a pre-combustion plasma jet igniter was designed, and its characteristics were examined from three aspects: the morphology, temperature, and discharge characteristics and process of ignition. Images of the OH distribution were obtained by using an OH Planar Laser-Induced Fluorescence(OH-PLIF) experimental system. Results have shown that the proposed plasma jet had a higher OH concentration, longer length, and larger area than those of a traditional igniter. The stability of discharge of the igniter was improved as the equivalence ratio φ was increased, and reducing gas flow reduced the pulsation of the plasma jet. When the input current was increased from 15A to 35 A, the highest average temperature increased from 5127 K to 7987 K. An increase in the equivalence ratio reduced the region of arc ionization, but expanded the regions of the core combustion reaction and the outer flame. Herein, this study has obtained a deep understanding of the jet and ignition law and developed a new idea for the application of plasma in the ignition field.A pre-combustion plasma jet igniter can significantly improve the efficiency of ignition and shorten the ignition process compared with a traditional igniter.

关键词： Pre-combustion plasma igniter Plasma Jet temperature field Ignition process Arc Flame

Morphological properties and proliferation analysis of olfactory ensheathing cells seeded onto three-dimensional collagen-heparan sulfate biological scaffolds

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Neural Regeneration Research 2012年第16期7卷 1213-1219页

作者： Na Liu Zhouping Tang Zhiyuan yu Minjie Xie yu zhang Erfang Yang Shabei Xu Department of Neurology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 HubeiProvince China

This study aimed to examine the differences in the morphological properties and proliferation of olfactory ensheathing cells in three-dimensional culture on collagen-heparan sulfate biological scaffolds and in two-dimensional culture on common flat culture plates. The proliferation rate of olfactory ensheathing cells in three-dimensional culture was higher than that in two-dimensional culture, as detected by an M-I-r assay. In addition, more than half of the olfactory ensheathing cells subcultured using the trypsinization method in three-dimensional culture displayed a spindly Schwann cell-like morphology with extremely long processes, while they showed a flat astrocyte-like morphology in two-dimensional culture. Moreover, spindle-shaped olfactory ensheathing cells tended to adopt an elongated bipolar morphology under both culture conditions. Experimental findings indicate that the morphological properties and proliferation of olfactory ensheathing cells in three-dimensional culture on collagen-heparan sulfate biological scaffolds are better than those in two-dimensional culture.

关键词： morphological properties cell proliferation biological scaffold olfactory ensheathing cells three-dimensional culture neural regeneration