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草原樱桃PGIP基因的克隆及生物信息学分析

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华北农学报 2012年第6期27卷 38-42页

作者：于文全刘海荣杨晓华赵恒田黑龙江省农业科学院牡丹江分院黑龙江牡丹江157041 中国科学院东北地理与农业生态研究所黑龙江哈尔滨150081

为了克隆草原樱桃多聚半乳糖醛酸酶抑制蛋白基因,并进行生物信息学分析。以草原樱桃叶片基因组为模板,PGIP基因保守序列设计引物,通过PCR扩增获得约1 kb的DNA片段。序列分析表明,该基因全长1 193 bp,包含有1个完整的开放阅读框,由2个外... 详细信息

为了克隆草原樱桃多聚半乳糖醛酸酶抑制蛋白基因,并进行生物信息学分析。以草原樱桃叶片基因组为模板,PGIP基因保守序列设计引物,通过PCR扩增获得约1 kb的DNA片段。序列分析表明,该基因全长1 193 bp,包含有1个完整的开放阅读框,由2个外显子和1个内含子构成,外显子总长990 bp,编码330个氨基酸,其编码的氨基酸序列中含有一段典型的亮氨酸重复序列,GenBank登录号为GU068977;该基因与中国李、杏、桃、马哈利樱桃、梅等李属植物的PGIP基因序列一致度达95%～99%。系统进化分析显示,属内亲缘关系较近、属间亲缘关系较远的特点。克隆了草原樱桃PGIP基因,为樱桃抗病育种提供一条新的基因资源。

关键词：草原樱桃 PGIP基因基因克隆生物信息学

Mechanism of action of Linggui Zhugan Decoction in treating non-alcoholic fatty liver disease using untargeted metabolomics approach

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Traditional Medicine Research 2025年第2期10卷 65-76页

作者： Huan Pei Ren-Lin Li Xin-Ran Song Qian-Qian Wan yu-Ming Wang Han-Zhou Li Wei-quan Xu Jia-Bao Liao Wei-Bo wen Jing Miao Huan-Tian Cui School of Basic Medicine Yunnan University of Chinese MedicineKunming 650599China School Clinical Medical College Yunnan University of Chinese MedicineKunming 650599China First School of Clinical Medicine Yunnan University of Chinese MedicineKunming 650599China School of Integrative Chinese and Western Medicine Tianjin University of Traditional Chinese MedicineTianjin 301617China Department of Pharmacy Tianjin Second People's HospitalTianjin 300192China

Background:Non-alcoholic fatty liver disease(NAFLD)is a liver disorder characterized by the accumulation and degeneration of fat in the liver cells,a condition that may further deteriorate and lead to cirrhosis and liver *** studies showed that metabolic dysfunction can promote NAFLD *** Zhugan Decoction(LGZGD)has therapeutic effects on *** mechanism of LGZGD still remains *** study was to examine the impact of LGZGD on the metabolic processes involved in the development of ***:A mice model of NAFLD was treated with *** therapeutic potential of LGZGD was evaluated by assessing the activity of transaminases,lipids levels of blood,and pathological changes in the liver of the mice model of ***,this study also evaluated the influence of LGZGD on liver inflammation and oxidative ***:The results of untargeted metabolomics analysis showed that LGZGD reduced the disordered lipid metabolism in NAFLD *** improved the oxidative stress and also reduced the levels of pro-inflammatory cytokines in the *** metabolomics analysis of liver samples revealed that LGZGD treatment improved metabolic disorders,including alanine,aspartate,glutamate,glycerophospholipid metabolism,and citrate *** RT-qPCR and Western blot results showed that LGZGD could regulate the expression of key enzymes in the metabolic pathway of the citrate cycle,including ATP-citrate lyase(ACLY),alanine-glyoxylate aminotransferase-2(AGXT2),phosphatidylethanolamine N-methyltransferase(PEMT),and succinate dehydrogenase(SDH).Conclusion:We found that LGZGD can treat NAFLD by reducing inflammatory responses,inhibiting oxidative stress,regulating alanine,aspartate,glutamate,and glycerophospholipid metabolism,and citrate cycle pathways.

关键词： NAFLD LGZGD untargeted metabolomics glycerophospholipid metabolism citrate cycle

Metal-organic frameworks with dinuclear metal centers for synergistically boosting CO2 photoreduction

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Science China Chemistry 2025年第01期 201-208页

作者： Hui-Feng Wang wen-Jie Shi yu-Xin Yang Bao-Xia Dong Tong-Bu Lu Di-Chang Zhong School of Chemistry and Chemical Engineering Yangzhou University Institute for New Energy Materials and Low Carbon Technologies School of Materials Science and Engineering Tianjin University of Technology

Dinuclear metal synergistic catalysis （DMSC） has been proved powerful in boosting CO2reduction,while this has not been achieved in heterogeneous catalysts with long-range order *** this study,we,by introduction of metal catalytic centers into an indium-based metal-organic framework （MOF）,obtained a series of isostructural In-MOFs,including three MOFs with mononuclear metal catalytic center （M-In）,three MOFs with dinuclear homometallic catalytic centers （MM-In）,and three MOFs with dinuclear heterometallic catalytic centers (MM’-In,M/M’=Co（II）,Ni（II）,Zn（II）).It was found that all these MOFs can serve as catalysts for photochemical CO2reduction to *** with M-In,the catalytic activities of MM-In were obviously enhanced,and that of MM’-In was further significantly *** example,the CO yield of Co Zn-In reaches a record value of 12,700μmol g-1h-1,which is 4.0,8.1,9.4,and 18.6-fold higher than that of Co Co-In,Zn Zn-In,Co-In,and Zn-In,***,the catalytic activities of Co Ni-In and Ni Zn-In also show different fold increase than those of corresponding dinuclear homometallic and mononuclear metallic *** results of control experiments and theoretical calculation reveal that the higher catalytic activities of MM-In over M-In can be ascribed to the DMSC effect,and the higher catalytic activity of MM’-In over MM-In can be ascribed to the strengthened DMSC *** work reveals the relationship between DMSC effect and bimetal centers in CO2reduction,which provides new insights into the DMSC and paves a new way to design highly active catalysts for photochemical CO2reduction.

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Comparative analysis of empirical and deep learning models for ionospheric sporadic E layer prediction

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Earth and Planetary Physics 2025年第1期9卷 10-19页

作者： BingKun yu PengHao Tian XiangHui Xue Christopher JScott HaiLun Ye JianFei Wu wen Yi TingDi Chen XianKang Dou Institute of Deep Space Sciences Deep Space Exploration LaboratoryHefei 230088China CAS Key Laboratory of Geospace Environment School of Earth and Space SciencesUniversity of Science and Technology of ChinaHefei 230026China Hefei National Laboratory University of Science and Technology of ChinaHefei 230088China Department of Meteorology University of ReadingReading RG66BBUK

Sporadic E(Es)layers in the ionosphere are characterized by intense plasma irregularities in the E region at altitudes of 90-130 *** they can significantly influence radio communications and navigation systems,accurate forecasting of Es layers is crucial for ensuring the precision and dependability of navigation satellite *** this study,we present Es predictions made by an empirical model and by a deep learning model,and analyze their differences comprehensively by comparing the model predictions to satellite RO measurements and ground-based ionosonde *** deep learning model exhibited significantly better performance,as indicated by its high coefficient of correlation(r=0.87)with RO observations and predictions,than did the empirical model(r=0.53).This study highlights the importance of integrating artificial intelligence technology into ionosphere modelling generally,and into predicting Es layer occurrences and characteristics,in particular.

关键词： ionospheric sporadic E layer radio occultation ionosondes numerical model deep learning model artificial intelligence

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湖缘峡谷及其含油性

地学前缘 2005年第3期12卷 186-194页

作者：姜在兴杨伟利于雯泉王卫红李守军李丕龙张善文中国地质大学(北京)能源学院北京100083 中国科学院广州地球化学研究所广东广州510640 中国科学院青岛海洋研究所山东青岛266071 中国石油大学(华东) 山东东营257061 胜利石油管理局山东东营257060

在陆相断陷湖盆陡坡带,控盆断裂的剧烈活动导致在相对较短时期内形成盆地的快速沉降,与湖盆边缘地形形成巨大高差。沿此边缘,经过早期阵发性洪水及河流的强烈下蚀,并不断向源侵蚀,在古断面上形成宽达数km,深达上km的深切谷,称之为湖缘... 详细信息

在陆相断陷湖盆陡坡带,控盆断裂的剧烈活动导致在相对较短时期内形成盆地的快速沉降,与湖盆边缘地形形成巨大高差。沿此边缘,经过早期阵发性洪水及河流的强烈下蚀,并不断向源侵蚀,在古断面上形成宽达数km,深达上km的深切谷,称之为湖缘峡谷。东营凹陷北部陡坡带的Y921峡谷即为湖缘峡谷,其现今形态为长7000m、宽3500m、深3500m、剖面呈“U”型的特征。古构造及古生物分析认为,在古新世至渐新世时期,陈南断层剧烈活动,使断层两侧形成较大的高差,在干旱、阵发性洪水侵蚀下逐渐形成Y921峡谷,并在其底部形成侵蚀面。孢粉分析认为,仅沙四上段沉积时期就有近1000m的高差。后期,随湖平面上升,峡谷逐渐充填了古近系沙河街组四段、三段和部分二段地层,主要为辫状河粗碎屑砂砾岩、扇三角洲相交错层理砂岩和滨浅湖相泥岩、粉砂岩。这种峡谷的特点是短、宽、深,其充填模式主要为辫状河-扇三角洲-湖泊相沉积体系。由于峡谷前缘为深湖相泥岩作为生油岩,陈南断层和峡谷内的冲刷面作为油气运移通道,峡谷内砂砾岩作为储层,因此在峡谷内形成了多种非构造油气藏类型。

关键词：湖缘峡谷断陷湖盆陡坡带控盆断层洪水侵蚀含油性

Cerebral fat embolism following autologous fat injection in facial reconstruction:A case report

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World Journal of Clinical Cases 2025年第2期13卷 50-58页

作者： Xiu-Ying Chen Fa Shen Chang Cheng yu-Han Wang wen-Chao Cheng De-Zhi yuan wen Huang Department of Neurology Chongqing Emergency Medical CenterChongqing University Central HospitalChongqing 400000China Department of Neurology Xinqiao HospitalThe Army Medical University(Third Military Medical University)Chongqing 400000China

BACKGROUND Autologous fat injection in facial reconstruction is a common cosmetic *** cerebral fat embolism(CFE)as a complication is rare,it carries serious health *** SUMMARY We present a case of a 29-year-old female patient who developed acute CFE following facial fat filling *** the surgery,the patient experienced symptoms including headache,nausea,vomiting,and difficulty breathing,which was followed by neurological symptoms such as slurred speech and left-sided *** physical examination and auxiliary investigations,including blood tests,head and neck computed tomography angiography,and cranial magnetic resonance diffusion-weighted imaging,were performed upon *** clinical diagnosis was acute cerebral embolism following facial fat filling *** included measures to improve cerebral circulation,dehydration for intracranial pressure reduction,nutritional support,and rehabilitation therapy for left limb *** patient showed a significant improvement in symptoms after 2 weeks of *** recovered left limb muscle strength to grade 5,had clear speech,and experienced complete relief of *** Our case highlights the potential occurrence of severe complications in patients undergoing fat injection in facial *** prevent these complications,plastic surgeons should enhance their professional knowledge and skills.

关键词： Cerebral fat embolism Mechanical thrombectomy Thrombolysis Steroid therapy Symptomatic treatment Case report

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茶产业与安溪县经济发展关联度的实证研究

茶叶科学 2012年第2期32卷 142-146页

作者：余文权孙威江吴国章赵丽红福建农林大学园艺学院福建福州350002 福建省农业厅福建福州350003

通过指标设计与数据收集,利用关联度计算方法,实证分析了茶产业与安溪县经济发展的关联度,研究表明:茶产业发展与安溪县总体经济、第一产业、第二产业和第三产业的发展均存在高度关联,茶产业的快速发展是推动安溪县经济发展的重要因素。

关键词：茶产业经济发展关联度

Drosophila models used to simulate human ATP1A1 gene mutations that cause Charcot-Marie-Tooth type 2 disease and refractory seizures

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Neural Regeneration Research 2025年第1期20卷 265-276页

作者： Yao yuan Lingqi yu Xudong Zhuang Dongjing wen Jin He Jingmei Hong Jiayu Xie Shengan Ling Xiaoyue Du wenfeng Chen Xinrui Wang Institute of Life Sciences College of Biological Science and EngineeringFuzhou UniversityFuzhouFujian ProvinceChina NHC Key Laboratory of Technical Evaluation of Fertility Regulation for Non-human Primate(Fujian Maternity and Child Health Hospital) FuzhouFujian ProvinceChina Medical Research Center Fujian Maternity and Child Health HospitalCollege of Clinical Medicine for Obstetrics&Gynecology and PediatricsFujian Medical UniversityFuzhouFujian ProvinceChina Department of Neurology and Institute of Neurology of The First Affiliated Hospital Institute of Neuroscienceand Fujian Key Laboratory of Molecular NeurologyFujian Medical UniversityFuzhouFujian ProvinceChina

Certain amino acids changes in the human Na^(+)/K^(+)-ATPase pump,ATPase Na^(+)/K^(+)transporting subunit alpha 1(ATP1A1),cause Charcot-Marie-Tooth disease type 2(CMT2)disease and refractory *** develop in vivo models to study the role of Na^(+)/K^(+)-ATPase in these diseases,we modified the Drosophila gene homolog,Atpα,to mimic the human ATP1A1 gene mutations that cause *** located within the helical linker region of human ATP1A1(I592T,A597T,P600T,and D601F)were simultaneously introduced into endogenous Drosophila Atpαby CRISPR/Cas9-mediated genome editing,generating the Atpα^(TTTF)*** addition,the same strategy was used to generate the corresponding single point mutations in flies(Atpα^(I571T),Atpα^(A576T),Atpα^(P579T),and Atpα^(D580F)).Moreover,a deletion mutation(Atpα^(mut))that causes premature termination of translation was generated as a positive *** these alleles,we found two that could be maintained as homozygotes(Atpα^(I571T)and Atpα^(P579T)).Three alleles(Atpα^(A576T),Atpα^(P579)and Atpα^(D580F))can form heterozygotes with the Atpαmut *** found that the Atpαallele carrying these CMT2-associated mutations showed differential phenotypes in *** heterozygous for Atpα^(TTTF)mutations have motor performance defects,a reduced lifespan,seizures,and an abnormal neuronal *** Drosophila models will provide a new platform for studying the function and regulation of the sodium-potassium pump.

关键词： ATP1A1 Atpα bang-sensitive paralysis Charcot-Marie-Tooth disease type 2 CRISPR/Cas9 homology-directed repair Na^(+)/K^(+)-ATPase point mutation seizures sodium pump

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非洲菊根腐病致病机理及防治的研究进展

福建农业学报 2014年第6期29卷 608-611页

作者：黄宇翔余文权张洪华福建省农业科学院作物研究所福建福州350013 福建省农业科学院花卉研究中心福建福州350013 福建省农业科学院福建福州350003

非洲菊是世界五大切花之一,具有很高的经济价值。随着种植面积的扩大,病害问题也越来越严重,根腐病是其中发生面积广、危害程度最大的主要病害之一。本文从寄主植物-病原菌相互作用的分子生物学角度出发,对非洲菊根腐病的发病机理、植... 详细信息

非洲菊是世界五大切花之一,具有很高的经济价值。随着种植面积的扩大,病害问题也越来越严重,根腐病是其中发生面积广、危害程度最大的主要病害之一。本文从寄主植物-病原菌相互作用的分子生物学角度出发,对非洲菊根腐病的发病机理、植物本身的抗病防卫机理以及目前防治措施进行了综述,并对非洲菊根腐病的抗性育种及未来的防治方法进行展望。

关键词：非洲菊根腐病隐地疫霉菌隐地蛋白病害防治