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Endoplasmic Reticulum Stress Is Involved in Hepatic SREBP-1c Activation and Lipid Accumulation in Fructose-Fed Mice

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Endoplasmic Reticulum Stress Is Involved in Hepatic SREBP-1c...

Facile synthesis of the dodecahydridododecaborate (B12H122-) from borane Lewis base adducts

中华预防医学会自由基预防医学专业委员会2012年夏季学术交流会

作者： ZHANG Cheng,CHEN Xi,ZHU Ren-min,ZHANG Ying,yu Tao,wang hua,ZHAO Hui, ZHAO Mei,Jl Yan-li,CHEN yuan-hua,MENG Xiu-hong,WEI Wei,XU De-xiang Department of Toxicology,Anhui Medical University,Hefei,230032

A link between fructose drinking and nonalcoholic fatty liver disease(NAFLD) has been demonstrated in human and rodent *** aim of the present study was to investigate whether endoplasmic reticulum(ER)stress is mediated in the development of fructose-induced *** CD-I mice were fed with 30%fructose solution for eight *** lipid accumulation was *** nuclear sterol regulatory element-binding protein(SREBP)-lc was *** showed that hepatic SREBP-1c was activated in mice fed with fructose *** acid synthase(fas)and acetyl-CoA carboxylase(ace),two target genes of SREBP-lc,were ***-evoked hepatic SREBP-lc activation seemed to be associated with insulin-induced gene(Insig)-l *** ER stress and unfolded protein response(UPR),as determined by an increased glucose-regulated protein(GRP78)expression and an increased eIF2a and PERK phosphorylation,were observed in liver of mice fed with fructose *** acid(PBA),an ER chemical chaperone,not only significantly attenuated ER stress,but also alleviated fructose-induced hepatic Insig-1 *** inhibited fructose-evoked hepatic SREBP-lc activation and the expression of SREBP-lc target genes,and protected against hepatic lipid *** conclusion,ER stress contributes,at least in part,to hepatic SREBP-lc activation and lipid accumulation in fructose-evoked NAFLD.

关键词： Fructose endoplasmic reticulum stress phenylbutyric acid hepatic lipid accumulation sterol regulatory element-binding protein

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Science China Chemistry 2025年

作者： Yi Jing Xin-Ran Liu Xing-hua wang Hui Han Sha-Sha Liang Xi-Meng Chen Dong-Hui Wei Lai-Sheng wang Xue-Nian Chen College of Chemistry Zhengzhou University School of Chemistry and Chemical Engineering Henan Key Laboratory of Boron Chemistry and Advanced MaterialsHenan Normal University Department of Chemistry Brown University

Polyhedral boranes are a class of well-known boron molecular clusters widely used in energy,chemistry,medicine,and materials science because of their unique physical and chemical *** efforts have been made in the past decades to find more effective synthetic methods for this important class of boron ***,existing synthetic methods suffer from low efficiency and low ***,we report a facile one-pot synthesis of[（CH3）3S]2B12H12with moderate yields at mild *** mechanisms for the multi-step chemoselective synthesis of B12H122-without other by-products are elucidated based on theoretical results and our previous *** Lewis base used in B–H bond condensation reaction,which acts as a hydrogen or to balance the newly generated polyhedral borane charges,is proposed and studied in *** current study has led to a more effective and selective synthetic method for B12H122-and has also implicated the syntheses of other new polyhedral boranes.

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Clinicopathological differences between patients with schistosomal appendicitis and non schistosomal appendicitis:A retrospectively study of past ten years

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World Journal of Clinical Cases 2025年第2期13卷 24-32页

作者： Xiao-Yi wang Yao Hao Zi-Jian wang Xiu-Liang Xu Jiang-hua Yang Department of Infectious Diseases The First Affiliated of Wannan Medical CollegeWuhu 241001Anhui ProvinceChina

BACKGROUND Chronic schistosomiasis causes multiple organ and multiple system diseases,especially the digestive *** eggs are mainly deposited in the stomach,liver and colorectal,but a few eggs are deposited in the appendix and cause *** present,there are few studies on schistosomal *** To explore the differences in epidemiological,clinical and pathological characteristics between schistosomal appendicitis and non-schistosomal appendicitis over the past decade in order to assess the impact of schistosomiasis on *** The differences of general data,clinical data and laboratory examination data of patients with appendicitis from October 2013 to October 2023 were retrospectively *** patients were divided into two groups for *** were 136 patients in schistosomal appendicitis group and 5418 patients in non-schistosomal appendicitis *** Schistosomal appendicitis accounted for 2.45%of all patients with appendicitis,and the annual proportion in the past decade was 2.2%,2.9%,1.8%,1.9%,3.4%,3.1%,1.9%,1.6%,3%,2.6%,*** prevalence of schistosomal appendicitis was middle-aged and elderly males,with an average age of 61.73±15.335 *** main population of non-schistosomal appendicitis was middle-aged men,with an average age of 35.8±24.013 years(P<0.001).The distribution of pathological types of appendicitis was different between the two groups(P<0.001).The incidence of acute suppurative appendicitis in non-schistosomal appendicitis was higher than that in schistosomal appendicitis[odds ratio(OR)=0.504;95%confidence interval(CI):0.349-0.728;P<0.001].The proportion of acute attack of chronic appendicitis in schistosomal appendicitis was higher than that in non-schistosomal appendicitis(OR=2.614;95%CI:1.815-3.763;P<0.001).The proportion of schistosomal appendicitis patients complicated with colorectal cancer was higher than that of nonschistosomal appendicitis patients(OR=5.087;95%CI:1.427-

关键词： Schistosomal appendicitis Schistosomiasis Appendix Colorectal cancer Clinicopathological characteristics

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计算机视觉镜头的精细调焦控制

微特电机 2002年第5期30卷 25-26页

作者：张玉华王安敏王成江青岛科技大学山东青岛266042

在步进电机控制系统中,采用调频调压与恒频脉冲调宽及高低压驱动相结合的连续细分驱动电路方法。LM331F/V变换器件提供细分时的参考电压,由软件控制产生频率信号,采用正弦与线性叠加规律对统组电流进行等步距细分,无需I/O扩展,工作可靠... 详细信息

在步进电机控制系统中,采用调频调压与恒频脉冲调宽及高低压驱动相结合的连续细分驱动电路方法。LM331F/V变换器件提供细分时的参考电压,由软件控制产生频率信号,采用正弦与线性叠加规律对统组电流进行等步距细分,无需I/O扩展,工作可靠,从而很好地满足了系统的要求。

关键词：计算机视觉镜头精细调焦控制步进电机控制系统

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武夷岩茶茶渣非水溶性膳食纤维提取工艺研究

食品研究与开发 2017年第2期38卷 42-47页

作者：杨宇华王淑培郑宝东王亚梅武夷学院茶与食品学院福建武夷山354300 福建农林大学食品科学学院福建福州350002 福建省武夷茶资源创新利用重点实验室福建武夷山354300

以冲泡后的岩茶茶渣为原料,采用酶法研究膳食纤维(IDF)的提取工艺参数,得出用α-淀粉酶处理茶粉的粗膳食纤维提取条件是:酶解温度55℃、酶添加量0.06 g/g、p H 9、酶解时间1 h、料液比1∶15(g/m L);木瓜蛋白酶精制IDF的最佳工艺条件是:... 详细信息

以冲泡后的岩茶茶渣为原料,采用酶法研究膳食纤维(IDF)的提取工艺参数,得出用α-淀粉酶处理茶粉的粗膳食纤维提取条件是:酶解温度55℃、酶添加量0.06 g/g、p H 9、酶解时间1 h、料液比1∶15(g/m L);木瓜蛋白酶精制IDF的最佳工艺条件是:酶解温度45℃、酶添加量0.004 g/g、p H值为4、酶解时间2 h、料液比1∶15(g/m L),此条件下膳食纤维的提取率最高,为97.12%。

关键词：武夷岩茶膳食纤维酶提取法优化工艺

Deciphering the role of mtH2O2 in hepatic ischemia-reperfusion injury mechanisms with low-background super-resolution fluorogenic probe

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Science China Chemistry 2025年第01期 297-307页

作者： Bin Fang Limin wang Haoqin Li Jiaxin Zhang Yang Ding Pan Pan Li Bo Peng hua Bai Lin Li Frontiers Science Center for Flexible Electronics Xi'an Institute of Flexible Electronics (IFE) and Xi'an Institute of Biomedical Materials &Engineering Northwestern Polytechnical University Institute of Flexible Electronics (IFE Future Technologies) Xiamen University

Hepatic ischemia-reperfusion injury（HIRI） is characterized by mitochondrial dysfunction and oxidative stress. Monitoring mitochondrial hydrogen peroxide（mtH2O2） levels in real-time through super-resolution imaging is crucial for elucidating its distribution in live cells and its mechanism of action during HIRI. However, low-background fluorogenic probes have been overlooked in the context of super-resolution imaging. In this study, we developed a low-background fluorogenic probe（MitoWG） with the potential for super-resolution morphology-correlated mitochondrial identification to track the fluctuates of mtH2O2in HIRI. Activation of the desirable fluorescence properties of the probe by mtH2O2was confirmed using structural illumination microscopy（SIM）, enabling high-quality mitochondrial imaging with exceptional specificity and *** in mtH2O2levels were successfully observed in both cellular and rat models of HIRI. Furthermore, we associated the decline in mitochondrial redox homeostasis with accelerated mtH2O2production during HIRI, which triggered mitophagy deficiency and led to cell death. In conclusion, Mito-WG possesses excellent photophysical and low-background properties for SIM imaging, making it a promising tool for mtH2O2tracking in HIRI research and clinical diagnosis.

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Small modifications of endo-functionalized cavities lead to large changes in molecular interaction in water

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Science China Chemistry 2025年第1期 369-376页

作者： Song-Meng wang Hao Nian Yan-Fang wang Li-Shuo Zheng yu-Tao Zheng Yi-Wei Dong Liping huang Xiaoping wang Wei Jiang Liu-Pan Yang Department of Chemistry Southern University of Science and Technology School of Chemistry and Pharmaceutical Sciences State Key Laboratory for Chemistry and Molecular Engineering of Medicinal ResourcesGuangxi Normal University School of Pharmaceutical Science Hengyang Medical School University of South China

Studying molecular recognition in aqueous environments is crucial for understanding biological processes. Herein, we present a series of N-doped endo-functionalized anthracene-based cavities by introducing inward-directed pyridine units. The subtle modifications can significantly affect the binding properties of these receptors, which is similar to the mutations in biological systems. Combining theoretical calculations and molecular recognition experiments demonstrated that these “mutations” have an impact on the hydrophobicity and charge distribution of the hosts, consequently significantly influencing molecular *** is crucial for understanding the intricate mechanisms of molecular-level interactions in biological receptors.

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Multivariate synergy for heightening ionic HOF robustness and proton conductivity

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Science China Chemistry 2025年

作者： Xiang-Tian Bai Li-Hui Cao Xu-Yong Chen Jia-yu wang Zi-Ye Zhou Shaanxi Key Laboratory of Chemical Additives for Industry College of Chemistry and Chemical EngineeringShaanxi University of Science and Technology

Multicomponent crystalline materials construct more complex and diversified structures with different combinations and modes,which expands the material structure space and application *** this work,we have successfully synthesized two twocomponent ionic hydrogen-bonded organic frameworks （iHOF-32 and iHOF-33） and a three-component iHOF （iHOF-34）.Guanidine hydrochloride which is rich in N and H and has a short chain length strengthens the hydrogen bonding network of iHOF,thereby aiding in the efficient migration of *** rigid architecture of 1,1'-diamino-4,4'-bipyridine diiodide furnishes additional scaffolding for the iHOFs and enhances its robustness under harsh ***-34 accomplishes the combination of the ultra-high proton conductivity of iHOF-32 and the robustness of iHOF-33 by the synergistic interaction among the three components of *** addition,iHOF-34 was fabricated as a composite membrane with a peak proton conductivity of 1.55×10-1S cm-1,which is 3.8 times higher than that of recast Nafion,and has extensive potential in fuel *** self-assembly strategy of multicomponent HOFs opens up new avenues for constructing diverse structures while promising new opportunities for applications in the fields of proton conduction and energy storage.

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小麦自噬相关因子ATG5的原核表达和抗血清制备

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生物技术通报 2017年第7期33卷 69-74页

作者：张加姿李开心于宝佳岳洁瑜王华忠天津师范大学生命科学学院天津市动植物抗性重点实验室天津300387

细胞自噬在植物生长发育和环境响应中扮演了重要角色,ATG5是参与自噬小体组装的核心因子之一。在前期工作中我们克隆了两个小麦ATG5基因TaATG5a和TaATG5b,并对其开展了初步的功能分析。鉴于后续基因功能研究中的免疫学方法涉及对特异性... 详细信息

细胞自噬在植物生长发育和环境响应中扮演了重要角色,ATG5是参与自噬小体组装的核心因子之一。在前期工作中我们克隆了两个小麦ATG5基因TaATG5a和TaATG5b,并对其开展了初步的功能分析。鉴于后续基因功能研究中的免疫学方法涉及对特异性抗体的需求,通过载体构建、原核表达和亲和层析过程获得了TaATG5a的重组蛋白,经兔免疫过程制备了TaATG5a的抗血清,采用ELISA和Western杂交等方法鉴定了抗体的效价和特异性。结果表明,克隆于pET30a载体上的TaATG5a在大肠杆菌中能够被IPTG高效诱导表达,重组蛋白的表观分子量与理论值基本一致,表达量在诱导0-8h范围内逐渐增加。纯化的重组蛋白纯度较高,满足抗体制备要求。制备的抗血清能特异性识别原核表达和小麦内源的TaATG5a,效价达到1∶25600。此外,制备的抗血清通过Western杂交还能够识别共价连接的小麦ATG12-ATG5复合物,该复合物是小麦叶片中ATG5的主要存在形式。

关键词：小麦自噬相关因子ATG5 原核表达抗血清