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Nitric Oxide Mediates Cytokinin Functions in Cell Proliferation and Meristem Maintenance in Arabidopsis

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Molecular Plant 2013年第4期6卷 1214-1225页

作者： Qi Shen yu-Ting wang hua Tian Fang-Qing Guo The National Key Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research (Shanghai) Institute of Plant Physiology andEcology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 300 Fenglin Road Shanghai 200032 China

Cytokinin and nitric oxide （NO） have been characterized as signaling molecules to trigger cell division in tissue culture. Here, we show that the hypocotyl and root explants of Arabidopsis NO-deficient mutant nosl/noal exhibit severe defects in callus induction and shoot regeneration in response to cytokinin. Accordingly, depletion of NO caused by a NO scavenger leads to a severe inhibitory effect on callus induction. Moreover, cytokinin-induced NO production is impaired in nosl/noal in which cytokinin-triggered activation of cell cycle gene CYCD3;1 is inhibited, indicating that NO may act downstream of cytokinin in the control of cell proliferation through CYCD3;1. This hypothesis is further con- firmed by the genetic evidence that constitutive expression of CYCD3;1 complements the defects of nosl/noal mutant in meristematic activity in shoot, root, and floral tissues as well as in cytokinin-induced callus initiation and shoot regen- eration. Furthermore, we show that NO deficiency caused by loss of NOSl/NOA1 impairs cellular development such as the duration of the mitotic phase and timing of the transition to endocycles in nosl/noal mutant leaves, which can be reverted by constitutive expression of CYCD3;1. Taken together, these results demonstrate that NO mediates transcrip- tional activation of CYCD3,1 in regulating the mitotic cycles in response to cytokinins.

关键词： nitric oxide cytokinins cell proliferation CYCD3 1 shoot regeneration endocycles.

Research progress on carbon materials as negative electrodes in sodium-and potassium-ion batteries

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Carbon Energy 2022年第6期4卷 1182-1213页

作者： Yang-yang Zhu yu-hua wang Yi-tong wang Tian-jie Xu Pei Chang Hubei Province Key Laboratory of Science in Metallurgical Process College of ScienceWuhan University of Science and TechnologyWuhanChina

Carbon materials,including graphite,hard carbon,soft carbon,graphene,and carbon nanotubes,are widely used as high-performance negative electrodes for sodium-ion and potassium-ion batteries(SIBs and PIBs).Compared with other materials,carbon materials are abundant,low-cost,and environmentally friendly,and have excellent electrochemical properties,which make them especially suitable for negative electrode materials of SIBs and *** with traditional carbon materials,modifications of the morphology and size of nanomaterials represent effective strategies to improve the quality of electrode *** nanostructures make different contributions toward improving the electrochemical performance of electrode materials,so the synthesis of nanomaterials is promising for controlling the morphology and size of electrode *** paper reviews the progress made and challenges in the use of carbon materials as negative electrode materials for SIBs and PIBs in recent *** differences in Na+and K+storage mechanisms among different types of carbon materials are emphasized.

关键词： carbon material graphene hard carbon negative electrode sodium/potassium-ion batteries

Numerical Simulation of Temperature Field and Thermal Stress Field of Work Roll During Hot Strip Rolling

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Journal of Iron and Steel Research International 2007年第5期14卷 18-21页

作者： LI Chang-sheng yu Hai-liang DENG Guan-yu LIU Xiang-hua wang Guo-dong State Key Laboratory of Rolling and Automation Northeastern University Shenyang 110004 Liaoning China

Based on the thermal conduction equations, the three-dimensional （3D） temperature field of a work roll was investigated using finite element method （FEM）. The variations in the surface temperature of the work roll during hot strip rolling were described, and the thermal stress field of the work roll was also analyzed. The results showed that the highest roll surface temperature is 593 ℃, and the difference between the minimum and maximum values of thermal stress of the work roll surface is 145.7 MPa. Furthermore, the results of this analysis indicate that temperature and thermal stress are useful parameters for the investigation of roll thermal fatigue and also for improving the quality of strip during rolling.

关键词： hot strip mill roll temperature field thermal stress finite element method

Third-order nonlinear optical properties of graphene composites:A review

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Chinese Physics B 2021年第8期30卷 120-131页

作者： Meng Shang Pei-Ling Li yu-hua wang Jing-Wei Luo Hubei Province Key Laboratory of Science in Metallurgical Process Wuhan University of Science and TechnologyWuhan 430081 China

Graphene has excellent thirdorder nonlinear optical(NLO)properties due to its unique electronic band structure and wideband gap *** paper focuses on the research progress of graphene and its composite materials in nonlinear optics in recent *** this review,recent results on graphene(or graphene oxide)-metal nanoparticles(G-MNPs),graphene-metal-oxide nanoparticles(G-MONPs),graphene-metal sulfide nanoparticles(G-MSNPs),and graphene-organic molecular composites(G-OM)have been *** addition,the enhancement mechanism of nonlinear absorption(NLA)and optical limiting(OL)have also been covered.

关键词： graphene composites nonlinear optics optical limiting enhancement

Forward and Backward Slip Models in MAS Rolling Process and Its On-Line Application

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Journal of Iron and Steel Research International 2007年第4期14卷 15-19页

作者： HU Xian-lei JIAO Zhi-jie HE Chun-yu LIU Xiang-hua wang Guo-dong State Key Laboratory of Rolling and Automation Northeastern University Shenyang 110004 Liaoning China

Based on the MAS rolling process in plate mill, the mathematical models of forward and backward slips of the wedges at plate head and tail were derived. According to the new model, the difference between the forward slip of the wedge and that of the normal part of the plate is obviously very small. The deviation is less than 2.5 % in general. Thus, in actual application, the forward slip of normal part of the plate can be used to calculate the length of rolled plate instead of the derived model. The rationality of this simplified method was confirmed with the application in Shougang 3 500 mm plate mill. The test results showed that the wedges of plate head and tail are symmetrical. The plate width deviation is greatly decreased by using the MAS method.

关键词： MAS plate forward slip backforward slip wedge

Synthesis of novel redox calix[4]arene derivative with 1,3-substituted large conjugated ferrocene bridge

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Chinese Chemical Letters 2009年第9期20卷 1039-1041页

作者： Bi Qiong Hong Fa Fu Yang Hong yu Guo Yan hua wang Xian Feng Chai College of Chemistry and Materials Fufian Normal University Fuzhou 350007 China

A novel redox calix[4]arene derivative with 1,3-substituted large conjugated ferrocene bridge was designed and synthesized in high yield. Its structure was characterized by element analysis, IR, ESI-MS, NMR spectrum, etc. The primary study of cyclic voltammetry showed it possessed excellent reversible electrochemistry response property. 2009 Fa Fu Yang. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.

关键词： Calixarene Ferrocene Synthesis Cyclic voltammetry

Improvement of the Injection and Transport Characteristics of Electrons in Organic Light-Emitting Diodes by Utilizing a NaC1 N-Doped Layer

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Chinese Physics Letters 2014年第4期31卷 178-181页

作者： XIAO Zhi-Hui WU Xiao-Ming hua yu-Lin wang Li BI Wen-Tao BAI Juan-Juan MU Xue YIN Shou-Gen School of Materials Science and Engineering Tianjin University of Technology Tianjin 300384Key Laboratory of Display Materials and Photoelectric Devices .Ministry of Education Tianjin 300384 Tianjin Key Laboratory of Photoelectric Materials and Devices Tianjin 300384

The injection and transport characteristics of electrons are enhanced by using sodium chloride （NaC1） as an n-type dopant doped into a 4, 7-diphnenyl-1, lO-phe-nanthroline （Bphen） electron-transporting layer, which improves the performance of organic light-emitting diodes （OLEDs）. Meanwhile, a NaCl-doped Bphen layer can effectively influence electrical characteristics of the devices, and significantly improve the current and power efficiency. The turn-on voltage and the operation voltage of the optimal device are decreased drastically from 6.5 V and 10.8 V to 3.3 V and 5 V, respectively, compared with those of the reference device. The maximum current efficiency and power efficiency of the optimal device are 7.0 cd/A and 4.4 Im/W at the current density of 16.70 mA/cm~, which are about I. 7 and 4 times higher than those of the reference device, respectively. Moreover, the enhancement of the injection and transport ability for electrons is attributed not only to the reduced energy barrier between A1 cathode and Bphen, but also to the increased mobility of electrons by the doping effect of NaCl. Therefore, both the electron injection and transport ability are enhanced, which improve the carrier balance in OLEDs and lead to the better device efficiency.

关键词： ORGANIC light emitting diodes SALT DOPING agents (Chemistry) ENERGY consumption ELECTRON mobility

Inducible nitric oxide synthase and Fas/FasL with C3 expression of mouse retinal pigment epithelial ceils in response to stimulation by peroxynitrite and antagonism of puerarin

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Chinese Medical Journal 2011年第16期124卷 2522-2529页

作者： HAO Li-na ZHANG Yan-qing SHEN yu-hua wang Zhi-yun wang Yan-hua Department of Ophthalmology Hebei Province People's HospitalShijiazhuang Hebei 050051 China

Background Retinal pigment epithelial （RPE） cell is a monolayer of multifunctional cells between the retina and the choroid. Peroxynitrite （ONOO-） is known to induce toxicity on RPE cells. This study aimed to evaluate ONOO- induced expression of inducible nitric oxide synthase （iNOS） and complement 3 （C3） via Fas/FasL pathway in RPE cells and the values of puerarin as a therapeutic target for inhibiting the apoptosis of RPE cells. Methods RPE cells were obtained from eyes of C57BL/6 mice. RPE cells were divided into control, ONOO-and puerarin groups. Control group was treated with saline, ONOO-group was treated with ONOO-, and puerarin group was treated with puerarin after added with ONOO-. All changes were observered at 6, 12 and 24 hours after treatment. Western blotting analysis was used to determine the expression of nitrotyrosine （NT, the foot print of ONOO-） and C3; flow cytometry was used to determine the apoptosis of RPE cells. Immunohistochemistry and Western blotting were used to determine Fas/FasL signal transduction. Gene array analysis, reverse transcription polymerase chain reaction （RT-PCR） and Western blotting were used to determine the expression of iNOS mRNA and iNOS protein in RPE cells. Results There were minor expression of NT, C3, Fas/FasL and iNOS mRNA in control group, and strong expression of NT and C3 in ONOO- group, while in puerarin group weak expressions of NT and C3 were detected as time passed by （P 〈0.001）. Apoptosis of RPE cells occured and reached a higher level at 6 and 24 hours after addition of ONOO- respectively in ONOO- group, but delayed apoptosis in puerarin group （P 〈0.05）. Compared to control group, the expression of Fas/FasL was up-regulated in ONOO- group, but was down-regulated in puerarin group （P 〈0.001）. Similarly, the expressions of iNOS mRNA and iNOS protein in ONOO-group were up-regulated in ONOO- group, but down-regulated in puerarin group （P 〈0.001）. Conclusions ONOO- expresseion in RPE cells may constitute the n

关键词： retinal pigment epithelial cells oxidative cell signal complement puerarin

Biomedical Image Processing Using FCM Algorithm Based on the Wavelet Transform

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Journal of Wuhan University of Technology(Materials Science) 2004年第3期19卷 18-20页

作者：闫玉华 Wuhan University of Technology

An effective processing method for biomedical images and the Fuzzy C-mean (FCM) algorithm based on the wavelet transform are *** using hierarchical wavelet decomposition, an original image could be decomposed into one lower image and several detail images. The segmentation started at the lowest resolution with the FCM clustering algorithm and the texture feature extracted from various sub-bands. With the improvement of the FCM algorithm, FCM alternation frequency was decreased and the accuracy of segmentation was advanced.

关键词： biomedical image processing FCM algorithm wavelet transform texture feature