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Analysis of Grassland Vegetation of the Southwest Heilongjiang Steppe （China） Using the Power Law

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Journal of Integrative Plant Biology 2005年第8期47卷 917-926页

作者： Mikinori TSUIKI yu-sheng wang Yiruhan Michio TSUTSUMI Masae SHIYOMI Faculty of Agriculture lwate University Morioka 020-8550 Japan National Northeast Agricultural University Harbin 150030 China Environmental Engineering Department College of Science and Engineering Jinan University Guangzhou 510630 China National Institute of Livestock and Grassland Science Ecology Department Tochigi 329-2724 Japan Faculty of Science lbaraki University Mito 310-8512 Japan

In 1997, we conducted a vegetation survey in three semi-arid natural grasslands （steppes） with different livestock grazing intensities in Southwest Heilongjiang Province, China, The dominant grassland species was the grass Stipa baicalensis Roshev. Grasslands with light, intermediate, and heavy grazing intensities were located 10, 5, and 2 km from a village, respectively. Villagers use the steppe to raise cattle, horses, sheep, and goats. Each of the three grasslands was surveyed by placing 100 quadrats （50 cm×50 cm） along a 50 m line transect. Each quadrat was divided into four equal areas （25 cm×25 cm; S-quadrats） and all plant species occurring in each of these smaller areas were identified and recorded. These data were summarized into frequency distributions and the percentage of S-quadrats containing a given species and the variance of each species were estimated. The power law was applied to these estimates. The power law was used to evaluate the spatial heterogeneity and frequency of occurrence for each species in the grassland community. The lightly grazed grassland exhibited high spatial heterogeneity （caused by large plant size）, the highest species diversity, and a high occurrence of S. baicalensis. In contrast, the heavily grazed grassland exhibited high spatial heterogeneity （caused by patchy populations of small plant size）, low species diversity, and a low occurrence of S. baicalensis. We judged that the heavily grazed grassland was overgrazed and exclusion of livestock from the degraded areas is necessary for recovery.

关键词： degradation grazing intensity Heilongjiang (China) spatial heterogeneity species diversity steppe Stipa baicalensis.

Estimation of Turnover and Equilibrium of Soil Organic Matter Using a Mathematical Approach

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Pedosphere 2006年第5期16卷 634-645页

作者： WU Chun-Yan CHEN Yi wang Jia-yu wang sheng-Jia Institute of Environmental Resources ＆ Soil Fertilizer Zhejiang Academy of Agricultural Sciences Hangzhou 310021（China）

The methods based on N uptake of aerial-plants, soil organic matter (SOM) dynamics, Jenny's equation, and actual measurement of long-term field experiments in Jiaxing, Quzhou, Huangyan and Hangzhou of Zhejiang Province, China were used to determine the organic mineralization rate being helpful in estimating the organic requirement for SOM equilibrium. The results showed that the estimated mineralization ratios of SOM for Jiaxing and Quzhou were, respectively, 0.0404 and 0.0508 based on N uptake of aerial-plants in non-fertilized plots; 0.0405 and 0.012 using SOM dynamics in non-fertilized plots; and 0.0413 and 0.0513 using the actual investigated data and Jenny's equation. With Jenny's equation, soil organic C balance in manure + N-P-K plots was estimated at nearly 28.8 g kg-1 for Jiaxing and 32.4 g kg-1 for Quzhou with predicted SOM linearly related to the actual investigated values (r2 = 0.9640 for Jiaxing and 0.8541 for Quzhou). To maintain the SOM balance in the non-fertilized plots the recommended rate of organic materials was 3 000-6 600 kg ha-1, and the relevant rates of farm yard manure (FYM) in the manure and N-P-K plots were estimated at 3 375 (dry) and 17670 kg ha-1 (wet) for Jiaxing, 1845 (dry) and 6090 kg ha-1 (wet) for Quzhou.

关键词： equilibrium mathematic modeling rice fields soil organic matter (SOM) turnover

Recurrent Seizures Manifestations in a Case of Congenital Hypoparathyroidism:a Case Report

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Chinese Medical Sciences Journal 2013年第4期28卷 242-244页

作者： sheng-yu wang Wei Wu Xuan Ma Internal Medicine Department Affiliated Hospital of Xi'an Medical University

HYPOPARATHYROIDISM is characterized by hypocalcemia, hyperphosphatemia and low or inappropriately normal levels of parathyroid hormone （PTH）. PTH is a key calcium regulatinghormone essential for calcium homeostasis, vitamin D-dependent calcium absorption, renal calcium reabsorption and renal phosphate clearance. Hypoparathyroidism may be due to congenital or acquired disorders. Causes include autoimmune diseases, genetic abnormalities, destruction or infiltrative disorders of the parathyroid glands.

关键词： hypoparathyroidism seizure basal ganglia calcification

Effect of mutant p27^(kip1) gene on human cholangiocarcinoma cell line, QBC_(939)

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World Journal of Gastroenterology 2008年第34期14卷 5344-5348页

作者： Jian Luo Yong-Jun Chen Wei-yu wang sheng-Quan Zou Department of General Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei Province China

AIM：To investigate the effects of exogenously mutated p27^kip1 （p27） on proliferation and apoptosis of human cholangiocarcinoma cell line, QBC939 in ***： Adenviral vectors were used to transfect mutated p27 cDNA into human QBC939 cell line. Expression of p27 was detected by RT-PCR. Western blot. Cell growth, morphological change, cell cycle, apoptosis and cloning formation were determined by MTT assay and flow ***： The expression of p27 protein and mRNA was increased signifi cantly in QBC939 cell line transfected with Ad-p27mt. The transfer of Ad-p27mt could signifi cantly inhibit the growth of QBC939 cells, decrease the cloning formation rate and induce apoptosis. p27 over expression caused cell cycle arrest at G0/G1 phase 72 h after infection with ***： p27 may cause cell cycle arrest at G0/G1 phase and subsequently lead to apoptosis. Recombinant adenovirus expressing mutant p27 may be potentially useful in gene therapy for cholangiocarcinoma.

关键词： Adenovirus Cholangiocarcinoma Gene therapy Cell cycle Apoptosis

Valproate reduces retinal ganglion cell apoptosis in rats after optic nerve crush

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Neural Regeneration Research 2023年第7期18卷 1607-1612页

作者： Feng Pan Dan Hu Li-Juan Sun Qian Bai yu-sheng wang Xu Hou Eye Institute of Chinese PLA and Department of Ophthalmology Xijing HospitalFourth Military Medical UniversityXi’anShaanxi ProvinceChina

The retinal ganglion cells of the optic nerve have a limited capacity for self-repair after *** is a histone deacetylase inhibitor and multitarget drug,which has been demonstrated to protect retinal *** this study,we established rat models of optic nerve-crush injury and injected valproate into the vitreous cavity immediately after *** evaluated changes in the ultrastructure morphology of the endoplasmic reticulum of retinal ganglion cells over time via transmission electron *** and western blot assay revealed that valproate upregulated the expression of the endoplasmic reticulum stress marker glucose-regulated protein 78 and downregulated the expression of transcription factor C/EBP homologous protein,phosphorylated eukaryotic translation initiation factor 2α,and caspase-12 in the endoplasmic reticulum of retinal ganglion *** findings suggest that valproate reduces apoptosis of retinal ganglion cells in the rat after optic nerve-crush injury by attenuating phosphorylated eukaryotic translation initiation factor 2α-C/EBP homologous protein signaling and caspase-12 activation during endoplasmic reticulum *** findings represent a newly discovered mechanism that regulates how valproate protects neurons.

关键词： apoptosis C/EBP homologous protein caspase-12 endoplasmic reticulum glucose-regulated protein 78 optic nerve crush phosphorylated eukaryotic translation initiation factor 2α retinal ganglion cells unfolded protein response valproate

In vivo bioluminescence imaging of hyperglycemia exacerbating stem cells on choroidal neovascularization in mice

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International Journal of Ophthalmology(English edition) 2016年第4期9卷 519-527页

作者： Xiang Gao yu wang Hui-yuan Hou Yang Lyu Hai-Yan wang Li-Bo Yao Jian Zhang Feng Cao yu-sheng wang Department of Ophthalmology Xijing Hospital Eye Institute of Chinese PLA Fourth Military Medical University State Key Laboratory of Cancer Biology Department of Biochemistry and Molecular Biology Fourth Military Medical University Department of Cardiology Xijing Hospital Fourth Military Medical University

AIM： To investigate the influence of hyperglycemia on the severity of choroidal neovascularization（CNV）,especially the involvement of bone marrow-derived cells（BMCs） and underlying mechanisms.·METHODS： BMCs from firefly luciferase（Fluc）/green fluorescent protein（GFP） double transgenic mice were transplanted into C57BL/6J wide-type mice. The recipient mice were injected intraperitoneally with streptozotocin（STZ） daily for 5 consecutive days to induce diabetes mellitus（DM）, followed by CNV laser *** BMCs recruitment in CNV exposed to hyperglycemia was firstly examined in Fluc/GFP chimeric mice by in vivo optical bioluminescence imaging（BLI） and in vitro Fluc assays. The CNV severity was evaluated by H＆E staining and choroidal flatmount. The expression of vascular endothelial growth factor（VEGF） and stromal cell derived factor-1（SDF-1） was detected by Western blot.·RESULTS： BLI showed that the BMCs exerted dynamic effects in CNV model in Fluc/GFP chimeric mice exposed to hyperglycemia. The signal intensity of transplanted Fluc＋GFP＋BMCs in the DM chimeric mice was significantly higher than that in the control chimeric mice with CNV induction at days 5, 7, 14 and 21（121861.67 ±9948.81 vs 144998.33 ±13787.13 photons/second/cm2/sr for control and DM mice, P5d〈0.05; 178791.67±30350.8 vs240166.67 ±22605.3, P7d〈0.05; 124176.67 ±16253.52 vs196376.67 ±18556.79, P14d〈0.05; 97951.60 ±10343.09 vs119510.00 ±14383.76, P21d〈0.05）, which was consistent with in vitro Fluc assay at day 7 [relative light units of Fluc（RLU1）], 215.00±52.05 vs 707.33±88.65, P 〈0.05; RLU1/relative light units of renilla luciferase（RLU2）, 0.90 ±0.17 vs 1.83 ±0.17, P 〈0.05]. The CNVs in the DM mice were wider than those in the control group at days 5, 7, 14 and21（147.83±17.36 vs 220.33±20.17 μm, P5d〈0.05; 212.17 ±24.63 vs 326.83 ±19.49, P7d〈0.05; 163.17 ±18.24 vs265.17 ±20.55, P14d〈0.05; 132.00 ±10.88 vs 205.33 ±12.98,P21d〈0.05）. The average area of CNV in the DM group was larger at 7d（20688.

关键词： hyperglycemia choroidal neovascularization bone marrow-derived cells molecular imaging in vivo optical bioluminescence imaging

Comparison of visual acuity of the patients on the first day after sub-Bowman keratomileusis or laser in situ keratomileusis

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International Journal of Ophthalmology(English edition) 2016年第3期9卷 448-452页

作者： Wei Zhao Ting Wu Ze-Hong Dong Jie Feng yu-Feng Ren yu-sheng wang Department of Ophthalmology Xijing Hospitalthe Fourth Military Medical University Out-patient Department the Second Artillery Engineering University

AIM：To compare recovery of the visual acuity in patients one day after sub-Bowman keratomileusis（SBK）or laser in situ keratomileusis（LASIK）.· METHODS：Data from 5923 eyes in 2968 patients that received LASIK（2755 eyes） or SBK（3168 eyes） were retrospectively *** eyes were divided into 4groups according to preoperative spherical equivalent：between-12.00 to-9.00 D,extremely high myopia（n =396,including 192 and 204 in SBK and LASIK groups,respectively）;-9.00 to-6.00 D,high myopia（n=1822,including 991 and 831 in SBK and LASIK groups,respectively）,-6.00 to-3.00 D,moderate myopia（n=3071,including 1658 and 1413 in SBK and LASIK groups,respectively）,and-3.00 to 0.00 D,low myopia（n =634,including 327 and 307 in SBK and LASIK groups,respectively）.Uncorrected logMAR visual acuity values of patients were assessed under standard natural *** of variance was used for comparisons among different groups.· RESULTS：Uncorrected visual acuity values were0.0115±0.1051 and 0.0466±0.1477 at day 1 after operation for patients receiving SBK and LASIK,respectively（P〈0.01）;visual acuity values of 0.1854±0.1842,0.0615±0.1326,-0.0033±0.0978,and-0.0164±0.0972 were obtained for patients in the extremely high,high,moderate,and low myopia groups,respectively（P〈0.01）.In addition,significant differences in visual acuity at day 1 after operation were found between patients receiving SBK and LASIK in each myopia subgroup.· CONCLUSION：Compared with LASIK,SBK is safer and more effective,with faster ***,SBK is more likely to be accepted by patients than LASIK for better uncorrected visual acuity the day following operation.

关键词： sub-Bowman keratomileusis laser in situ keratomileusis visual acuity myopia recovery

Molecular Genetic Variation in a Clonal Plant Population of Leymus chinensis （Trin.） Tzvel.

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Journal of Integrative Plant Biology 2005年第9期47卷 1055-1064页

作者： yu-sheng wang Li-Ming ZHAO Hua wang Jie wang Da-Ming HUANG Rui-Min HONG Xiao-Hua TENG Nakamura MIKI Grassland Research Station College of Animal Science and Technology Northeast Agricultural University Harbin 150030 China The School of Management Tianjin University Tianjin 300071 China School of Economy-Management Tianjin University of Technology Tianjin 300191 China Department of Breast Surgery Cancer Institute Fu-Dan University Medical Center Shanghai 200032 China Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China

Randomly amplified polymorphic DNA （RAPD） analysis was used to investigate the genetic variation among populations, between populations, and within populations, relationships between genetic distance and geographic distance, and the molecular variation and population size. The effects of geographic and genetic distances, as well as of genetic differentiation and population size, on genetic variations of Leymus chinensis （Trin.） Tzvel. are discussed. The present study showed that there was significant RAPD variation between the Baicheng region population and the Daqing region population, with a molecular variance of 6.35% （P 〈 0.04）, and for differentiation among area populations of the Daqing region, with a molecular variance of 8.78% （P 〈 0.002）. A 21.06% RAPD variation among all 16 populations among two regions was found （P 〈 0.001）, as well as 72.59% variation within populations （P 〈 0.001）. Molecular variation within populations was significantly different among 16 populations.

关键词： clonal plant population genetic distance genetic variation geographic distance molecular variation.

A Trojan horse biomimetic delivery system using mesenchymal stem cells for HIF-1α siRNA-loaded nanoparticles on retinal pigment epithelial cells under hypoxia environment

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International Journal of Ophthalmology(English edition) 2022年第11期15卷 1743-1751页

作者： Lei Zhang Jie-Jing Yan Hai-Yan wang Mu-Qiong Li Xi-Xi wang Li Fan yu-sheng wang Shaanxi Eye Hospital Xi'an People's Hospital(Xi'an Fourth Hospital)Xi'an 710004Shaanxi ProvinceChina Department of Ophthalmology Xijing HospitalXi'an 710032Shaanxi ProvinceChina Ophthalmology Department Xi'an No.1 Hospitalthe First Affiliated Hospital of Northwest UniversityXi'an 710002Shaanxi ProvinceChina Department of Pharmaceutical Chemistry and Analysis School of Pharmacy Air Force Medical University Xi'an 710032Shaanxi ProvinceChina Department of Mathematics and Statistics University of Arkansas at Little RockLittle RockAR 72204USA

AIM: To demonstrate the feasibility of mesenchymal stem cell(MSC)-mediated nano drug delivery, which was characterized by the “Trojan horse”-like transport of hypoxiainducible factor-1α small interfering RNA(HIF-1α si RNA) between MSCs and retinal pigment epithelial cells(RPE) under hypoxia ***: Plasmid and lentivirus targeting the human HIF-1α gene were designed and constructed. HIF-1α si RNA was encapsulated into poly(lactic-co-glycolic acid) nanoparticles(PLGA-NPs) through the water-in-oil-in-water(w/o/w) multiple emulsion technique. The effect of PLGANPs uptake on the expression of HIF-1α m RNA was tested in RPE cells by real-time quantitative polymerase chain reaction(q PCR) and additional transfected conditions were used as control, including lentivirus group, nude plasmid group and blank PLGA group. MSCs were transfected with the NPs and the transfection efficacy was evaluated by flow cytometry. Transwell co-culture system of transfected MSCs and RPE cells was constructed under hypoxia environment. The effects of MSC-loaded HIF-1α si RNA PLGA-NPs on proliferation, apoptosis, and migration of RPE cells were then evaluated. The effect of transfected MSCs on HIF-1α expression of RPE cells was analyzed by using q PCR at the time points 24h, 3d, and ***: The average diameter of PLGA-NPs loaded with HIF si RNA was 314.1 nm and the zeta potential was-0.36 m V. The transfection efficiency of PLGA-NPs was 67.3%±5.2% into MSCs by using flow cytometry. Compared with the lentivirus group, the PLGA-NPs loaded with HIF-1α si RNA can effectively reduce the expression of HIF-1α m RNA up to 7d in RPE(0.63±0.05 at 7d, P<0.001). In the Transwell co-culture system of transfected MSCs and RPE, the abilities of proliferation(2.34±0.17, 2.40±0.28, 2.47±0.24 at 48h, F=0.23, P=0.80), apoptosis(14.83%±2.43%, 12.94%±2.19%, 12.39%±3.21%;F=0.70, P=0.53) and migration(124.5±7.78, 119.5±5.32, 130±9.89, F=1.33, P=0.33) of the RPE cells had no differences between MSClo

关键词： hypoxia mesenchymal stem cells poly(lactic-co-glycolic acid)nanoparticles hypoxia-inducible factor-1α retinal pigment epithelial cells