检索结果-南通市图书馆

A mouse model for SARS-CoV-2-induced acute respiratory distress syndrome

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2021年第2期6卷 223-225页

作者： Weiqi Hong Jingyun Yang Zhenfei Bi Cai He Hong Lei wenhai yu yun Yang Changfa Fan Shuaiyao Lu Xiaozhong Peng Xiawei Wei Laboratory of Aging Research and Cancer Drug Target State Key Laboratory of Biotherapy and Cancer CenterNational Clinical Research Center for GeriatricsWest Chino HospitalSichuan UniversityNo.17Block 3Southern Ren min Rood610041 ChengduSichuanPeople's Republic of China National Kunming High-level Biosafety Primate Research Center Institute of Medical BiologyChinese Academy of Medical Sciences and Peking Union Medical CollegeYun nonChina Division of Animal Model Research Institute for Laboratory Animal ResourcesNational Institutes for Food and Drug Control102629 BeijingChina State Key Laboratory of Medical Molecular Biology Deportment of Molecular Biology and BiochemistryInstitute of Basic Medical SciencesMedical Primate Research CenterNeuroscience CenterChinese Academy of Medical SciencesSchool of Basic Medicine Peking Union Medical CollegeBeijingChina

Dear Editor,The COVID-19 pandemic has covered more than 200 countries and regions around the world since its outbreak in January *** date,the SARS-CoV-2 virus has caused>1.2 million *** mortality rate of COVID-19 is closely concerned with the clinical symptoms of the patients from mild-to-severe ***,in its most severe form,COVID-19 leads to life-threatening pneumonia and acute respiratory distress syndrome(ARDS),which is mostly accom-panied by a hyperactive immune response called"cytokine storm"and has high death rates from 40 to 50%.

关键词： distress acute respiratory

Splicing factor SF3B3,a NS5-binding protein,restricts ZIKV infection by targeting GCH1

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Virologica Sinica 2023年第2期38卷 222-232页

作者： Tanxiu Chen Hao Yang Penghui Liu Moliduer Hamiti Xintian Zhang Yi Xu Wenqi Quan Yong Zhang wenhai yu Li Jiao Tingfu Du Juemin Xi Bin Yin Wei Zhou Shuaiyao Lu Xiaozhong Peng Institute of Medical Biology Chinese Academy of Medical Sciences and Peking Union Medical CollegeKunming650031China Department of Science and Education Jiangxi Key Laboratory of Translational Cancer ResearchJiangxi Cancer HospitalNanchang330029China The State Key Laboratory of Medical Molecular Biology Department of Molecular Biology and BiochemistryInstitute of Basic Medical SciencesMedical Primate Research CenterNeuroscience CenterChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing100730China

Zika virus(ZIKV),a positive-sense single-stranded RNA virus,causes congenital ZIKV syndrome in children and Guillain-Barre Syndrome(GBS)in *** expresses nonstructural protein 5(NS5),a large protein that is essential for viral *** NS5 confers the ability to evade interferon(IFN)signalling;however,the exact mechanism remains *** this study,we employed affinity pull-down and liquid chromatography-tandem mass spectrometry(LC-MS/MS)analyses and found that splicing factor 3b subunit 3(SF3B3)is associated with the NS5-Flag pull-down complex through interaction with *** assays showed that SF3B3 overexpression inhibited ZIKV replication by promoting IFN-stimulated gene(ISG)expression whereas silencing of SF3B3 inhibited expression of ISGs to promote ZIKV *** cyclohydrolase I(GCH1)is the first and ratelimiting enzyme in tetrahydrobiopterin(BH4)***5 upregulates the expression of GCH1 during ZIKV *** GCH1 marginally promoted ZIKV replication via the IFN ***,GCH1 expression is related to the regulation of *** of the SF3B3 protein effectively reduced GCH1 protein levels,whereas SF3B3 knockdown increased its *** findings indicated that ZIKV NS5 binding protein SF3B3 contributed to the host immune response against ZIKV replication by modulating the expression of GCH1.

关键词： Nonstructural protein 5(NS5) Splicing factor 3b subunit 3(SF3B3) GTP cyclohydrolase I(GCH1) IFN-stimulated gene(ISGs) IFN signalling Pathway

Differential Transcriptomic Landscapes of SARS-CoV-2 Variants in Multiple Organs from Infected Rhesus Macaques

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genomics, Proteomics & Bioinformatics 2023年第5期21卷 1014-1029页

作者： Tingfu Du Chunchun Gao Shuaiyao Lu Qianlan Liu yun Yang wenhai yu Wenjie Li Yong Qiao Sun Cong Tang Junbin Wang Jiahong Gao Yong Zhang Fangyu Luo Ying Yang yun-Gui Yang Xiaozhong Peng National Kunming High-level Biosafety Primate Research Center Institute of Medical BiologyChinese Academy of Medical Sciences and Peking Union Medical CollegeKunming 650118China State Key Laboratory of Medical Molecular Biology Department of Molecular Biology and BiochemistryInstitute of Basic Medical SciencesMedical Primate Research CenterNeuroscience CenterChinese Academy of Medical SciencesSchool of Basic MedicinePeking Union Medical CollegeBeijing 100005China CAS Key Laboratory of Genomic and Precision Medicine Collaborative Innovation Center of Genetics and DevelopmentCollege of Future TechnologyBeijing Institute of GenomicsChinese Academy of Sciences and China National Center for BioinformationBeijing 100101China Sino-Danish College University of Chinese Academy of SciencesBeijing 100049China Institute of Stem Cell and Regeneration Chinese Academy of SciencesBeijing 100101China Institute of Laboratory Animal Science Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China

Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)caused the persistent coronavirus disease 2019(COVID-19)pandemic,which has resulted in millions of deaths worldwide and brought an enormous public health and global economic *** recurring global wave of infections has been exacerbated by growing variants of *** this study,the virological characteristics of the original SARS-CoV-2 strain and its variants of concern(VOCs;including Alpha,Beta,and Delta)in vitro,as well as differential transcriptomic landscapes in multiple organs(lung,right ventricle,blood,cerebral cortex,and cerebellum)from the infected rhesus macaques,were *** original strain of SARS-CoV-2 caused a stronger innate immune response in host cells,and its VOCs markedly increased the levels of subgenomic RNAs,such as N,Orf9b,Orf6,and Orf7ab,which are known as the innate immune antagonists and the inhibitors of antiviral ***,the original SARS-CoV-2 strain and Alpha variant induced larger alteration of RNA abundance in tissues of rhesus monkeys than Beta and Delta variants ***,a hyperinflammatory state and active immune response were shown in the right ventricles of rhesus monkeys by the up-regulation of inflammation-and immune-related ***,peripheral blood may mediate signaling transmission among tissues to coordinate the molecular changes in the infected ***,these data provide insights into the pathogenesis of COVID-19 at the early stage of infection by the original SARS-CoV-2 strain and its VOCs.

关键词： SARS-CoV-2 Variant of concern Subgenomic RNA Rhesus macaque Transcriptome

不确定DoS攻击下的异构多智能体系统异步控制器设计

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

哈尔滨工业大学学报 2021年第8期53卷 153-162页

作者：倪洪杰俞文海张丹浙江工业大学信息工程学院杭州310023

研究了存在不确定拒绝服务(denial of service,DoS)攻击的异构多智能体系统协同控制问题。网络环境的开放性会导致网络攻击的复杂性不断提高,其中,对于一类不确定网络攻击的研究具有重要的现实意义。由于不确定攻击情况下模态获取困难,... 详细信息

研究了存在不确定拒绝服务(denial of service,DoS)攻击的异构多智能体系统协同控制问题。网络环境的开放性会导致网络攻击的复杂性不断提高,其中,对于一类不确定网络攻击的研究具有重要的现实意义。由于不确定攻击情况下模态获取困难,将导致控制器模态与系统模态产生不匹配问题。首先,正常情况下所有智能体都是时间同步的并且以固定采样周期相互通信,当攻击发生时采用保持输入机制并且假定攻击持续时间是有界的,通过使用马尔可夫切换系统方法来构建该复杂动态系统模型。其次,通过解耦技术将原高维系统转换为两个低维的闭环误差系统,并通过Lyapunov稳定性理论得到了保证异构多智能体系统输出一致性的充分条件。进一步,应用相关矩阵变换方法给出了通过求解一系列矩阵不等式来获得控制器增益的方法。最后,通过基于移动舞台机器人系统的仿真研究验证了本文所提出方法的有效性。与现有结果相比,本文所考虑的攻击概率可以是不确定甚至完全未知的,所设计的异步控制器具有更好的兼容性,即包含了常见的同步控制器以及模型独立控制器。

关键词：异构多智能体系统不确定DoS攻击异步控制马尔可夫切换系统 Lyapunov理论

Study of yttrium and cerium on the oxidation resistance of silicide coatings prepared on Ti-6Al-4V alloy by pack-cementation process

在线全文

学校读者我要写书评

暂无评论

Journal of Rare Earths 2015年第2期33卷 221-226页

作者：于文海田进田伟赵俊李涌泉刘言正 China United Northwest Institute for Design and Research School of Aeronautics Northwestern Polytechnical University School of Environmental & Municipal Engineering Xi'an University of Architecture and Technology

Pure silicide coating and Y-Ce modified silicide coating were prepared on Ti-6Al-4V alloy by pack-cementation process. The structures as well as the isothermal oxidation behaviors of the coatings were comparatively studied. The results showed that both pure silicide coating and Y-Ce modified silicide coating prepared at 1080℃ for 4 h were composed of a TiSi2 outer layer, a TiSi middle layer and a Ti5Si4 inner layer. The oxidation tests showed that the Y-Ce modified silicide coating possessed much better oxidation resistance than the pure silicide coating at 1000℃, implying the beneficial effects of Y and Ce on the oxidation resistance of the coating.

关键词： Ti-6Al-4V alloy silicide coating oxidation resistance Y-Ce modification rare earths

在线全文

学校读者我要写书评

暂无评论

葛洪的炼丹术及其对中医药学的贡献研究

江苏中医药 2008年第8期40卷 10-12页

作者：余文海江西省儿童医院江西南昌330006

中医药学的形成和发展有着多方面的因素,其中"儒、道、佛"三大教派是中医药学形成和发展的主体,而又以佛教和道教为其核心。葛洪是中国历史上儒、道兼综的杰出代表人物,他以炼丹为毕生事业,他精辟的炼丹术理论和高超的炼丹技术是他成就... 详细信息

中医药学的形成和发展有着多方面的因素,其中"儒、道、佛"三大教派是中医药学形成和发展的主体,而又以佛教和道教为其核心。葛洪是中国历史上儒、道兼综的杰出代表人物,他以炼丹为毕生事业,他精辟的炼丹术理论和高超的炼丹技术是他成就医业的坚强基石,从而丰富和发展了中医药学。他的炼丹术也为中药化学的研究开创了先河;由他的炼丹术演绎出来的"内丹修炼法"为气功的起源开辟了道路。这是葛洪炼丹术的两大突出贡献。

关键词：葛洪炼丹术中医药学气功

在线全文

学校读者我要写书评

暂无评论

基于对称性评估的保偏光纤偏振轴检测技术

光学学报 2019年第2期39卷 109-118页

作者：于文海冯迪梁日宋凝芳北京航空航天大学仪器科学与光电工程学院北京100083

提出一种基于端面图像对称性特征的偏振轴检测算法。该算法利用图像极坐标变换来实现图像的对称性检测,使用金字塔搜索法快速寻找最优对称轴,确定偏振轴向。所提算法利用图像的全局性特征而不依赖于应力区边缘点位置实现精确定位,对图... 详细信息

提出一种基于端面图像对称性特征的偏振轴检测算法。该算法利用图像极坐标变换来实现图像的对称性检测,使用金字塔搜索法快速寻找最优对称轴,确定偏振轴向。所提算法利用图像的全局性特征而不依赖于应力区边缘点位置实现精确定位,对图像清晰度要求显著降低。实验结果表明,所提算法具有较高的准确性和精确性(±0.1°),其稳健性大幅增强,且算法速度提升了近1.5倍。

关键词：图像处理对称性端面图像保偏光纤偏振轴

Immunogenicity and protective efficacy of a recombinant protein subunit vaccine and an inactivated vaccine against SARS-CoV-2 variants in non-human primates

在线全文

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2022年第4期7卷 1170-1180页

作者： Qian He Qunying Mao Xiaozhong Peng Zhanlong He Shuaiyao Lu Jialu Zhang Fan Gao Lianlian Bian Chaoqiang An wenhai yu Fengmei Yang Yanan Zhou yun Yang Yanyan Li Yadi yuan Xujia Yan Jinghuan Yang Xing Wu Weijin Huang Changgui Li Junzhi Wang Zhenglun Liang Miao Xu Division of Hepatitis and Enterovirus Vaccines Institute of Biological ProductsNational Institutes for Food and Drug ControlNHC Key Laboratory of Research on Quality and Standardization of Biotech ProductsNMPA Key Laboratory for Quality Research and Evaluation of Biological ProductsBeijing 102629People’s Republic of China Institute of Medical Biology Chinese Academy of Medicine Sciences&Peking Union Medical CollegeKunming 650118People’s Republic of China

Emerging SARS-CoV-2 variants and the gradually decreasing neutralizing antibodies over time post vaccination have led to an increase in incidents of breakthrough infection across the *** investigate the potential protective effect of the recombinant protein subunit COVID-19 vaccine targeting receptor-binding domain(RBD)(PS-RBD)and whole inactivated virus particle vaccine(IV)against the variant strains,in this study,rhesus macaques were immunized with PS-RBD or IV vaccine,followed by a Beta variant(B.1.351)*** neutralizing activity against the Beta variant was reduced compared with that against the prototype,the decreased viral load in both upper and lower respiratory tracts,milder pathological changes,and downregulated inflammatory cytokine levels in lung tissues after challenge demonstrated that PS-RBD and IV still provided effective protection against the Beta variant in the macaque ***,PS-RBD-induced macaque sera possessed general binding and neutralizing activity to Alpha,Beta,Delta,and Omicron variants in our study,though the neutralizing antibody(NAb)titers declined by varying degrees,demonstrating potential protection of PS-RBD against current circulating variants of concern(VOCs).Interestingly,although the IV vaccine-induced extremely low neutralizing antibody titers against the Beta variant,it still showed reduction for viral load and significantly alleviated pathological *** correlates of vaccine-induced protection(CoP)like antibody-dependent cellular cytotoxicity(ADCC)and immune memory were both confirmed to be existing in IV vaccinated group and possibly be involved in the protective mechanism.

关键词： protective vaccine neutral

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

天然丝光沸石对无机盐的复合研究

无机材料学报 1991年第3期6卷 300-305页

作者：丁屹俞文海中国科学技术大学材料科学与工程系

本文报道天然丝光沸石对无机盐的复合现象。利用 XRD、IR 及电性能测量等实验手段对无机盐复合丝光沸石的结构及电性能进行了研究,表明无机盐可以离子形式或松散的分子形式存在于丝光沸石的内部孔道及沸石颗粒的表面;在较高温度下制备... 详细信息

本文报道天然丝光沸石对无机盐的复合现象。利用 XRD、IR 及电性能测量等实验手段对无机盐复合丝光沸石的结构及电性能进行了研究,表明无机盐可以离子形式或松散的分子形式存在于丝光沸石的内部孔道及沸石颗粒的表面;在较高温度下制备的样品中,沸石颗粒表面有α-Fe_2O_3存在;研究还表明在无机盐复合丝光沸石中形成了骨架羟基,且样品的结构与丝光沸石基本相同,其电导率可达10^(-4)(cm·Ω)^(-1)的量级。

关键词：无机盐丝光沸石复合孔道骨架