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Bone marrow mesenchymal stem cell-induced autophagy ameliorates TNBS-induced experimental colitis by downregulating the NLRP3 inflammasome

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BIOCELL 2023年第12期47卷 2627-2639页

作者： JINJIN FU XIAOyuE FENG JUAN WEI XIANG GENG yu gong FENGDONG LI SHAOHUA ZHUANG JIN HUANG FANGyu WANG Department of Gastroenterology The Affiliated Changzhou No.2 People’s HospitalNanjing Medical UniversityChangzhouChina Department of Gastroenterology Jinling HospitalNanjing Medical UniversityNanjingChina

This study aimed to elucidate the potential mechanisms through which bone marrow-derived mesenchymal stem cells(BM-MSCs)may be effective in alleviating experimental colitis induced by treatment with 2,4,6-trinitrobenzene-sulfonate acid(TNBS),specifically through autophagy ***:BM-MSCs were collected from BALB/c mice for subsequent *** study employed cell counting kits(CCK-8)to investigate the impact of the MSC-conditioned medium(M medium)on the proliferation of RAW264.7 *** GFP-mRFP-LC3 adenovirus was transfected into RAW264.7 to detect autophagic *** gene expression of cytokines was assessed through quantitative reverse transcription polymerase chain reaction(qRT-PCR).Western blot analysis was employed to determine the presence of a binding interaction between NOD-like receptor protein 3(NLRP3)and ***,a colitis mouse model was established by TNBS *** disease activity score was assessed regularly,and histological and morphometric analyses were performed on colonic *** serum cytokines were identified using an enzyme-linked immunosorbent ***:BM-MSCs significantly promoted the proliferation of *** vitro lipopolysaccharide(LPS)-stimulated RAW264.7 cells,treated with BM-MSCs,triggered autophagy and inhibited cytokine mRNA ***,in LPS-induced RAW264.7,BM-MSCs enhanced the Beclin1 protein expression and the microtubule-associated protein 1 light chain 3(LC3)-II to LC3-I ratio while suppressing the protein levels of NLRP3 and apoptosis-associated speck-like protein(ASC).Nevertheless,3-methyladenine(3-MA),an inhibitor of autophagy,prevented the impact of BM-MSCs by reducing the levels of NLRP3 and ASC proteins,suggesting that autophagy triggered the inhibition of the NLRP3 *** comparison to the mice in the TNBS group,the mice in the TNBS+MSC group displayed a more acute form of colitis,and the IL1βand IL18 cytokines in their serum were

关键词： RAW264.7 3-Methyladenine(3-MA) IL1β IL18

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Uveal effusion following acute primary angle-closure:a retrospective case series

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International Journal of Ophthalmology(English edition) 2017年第3期10卷 406-412页

作者： Jian-Gang Yang Jian-Jun Li Hua Tian Yan-Hong Li yu-Jing gong An-Le Su Na He Xi’an Eye Hospital Xi’an First Hospital Department of Ophthalmology Xian XD Group Hospital Department of Ophthalmology the Second Affiliated Hospital of Xi’an Jiaotong University

AIM：To evaluate the morphological changes in anterior segment in Chinese patients with uveal effusion（UE）after the attack of acute primary angle-closure（APAC）using ultrasound biomicroscopy（UBM）,and to assess the clinical course and prognosis of the ***：In a retrospective case series,26 eyes in 26 consecutive patients diagnosed with UE after the treatment of intraocular pressure（IOP）-lowering medication for the attack of APAC were enrolled. The unaffected fellow eyes served as controls. The morphological changes were observed by ultrasonography,slit lamp microscopy and gonioscopy. UBM was used to assess the degree and extent of effusion based on the analysis of parameters associated with ***：The mean IOP was 9.2（SD 2.1）mm Hg at the diagnosis of UE after IOP-lowering medication,while 14.1（SD,2.6）mm Hg in the fellow eyes（P=0.000）. The anterior chamber depth（ACD）（P=0.000）,angle opening distance at 500 μm（AOD500）（P〈0.01）and anterior chamber angle（ACA）（P〈0.05）were decreased significantly,while ciliary body thickness（CBT）（P〈0.05）increased significantly in UE eyes. UE grade analysis showed 7 eyes in grade 1,9 eyes in grade 2,and 10 eyes in grade 3. Quadrant scores were performed of 4 eyes in 1 quadrant,3 eyes in 3 quadrants,and 19 eyes in 4 quadrants. There was the positive correlation between grade and quadrant score（R=0.644,P=0.000）. The effusion on all eyes were recovered after medication,which mean IOP was 13.9（SD,2.8）mm ***：UE is a frequent complication in Chinese patients after the attack of APAC,partially associated with hypotony. The severity of UE is correlation with height of effusion,extent of detachment,and shallower ACD.

关键词： uveal effusion acute primary angle-closure intraocular pressure ultrasound biomicroscopy

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Faecalibacterium prausnitzii supernatant ameliorates dextran sulfate sodium induced colitis by regulating Th17 cell differentiation

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World Journal of Gastroenterology 2016年第22期22卷 5201-5210页

作者： Xiao-Li Huang Xin Zhang Xian-Yan Fei Zhao-Gui Chen Yan-Ping Hao Shu Zhang Ming-Ming Zhang Yan-Qiu yu Cheng-gong yu Department of Gastroenterology Gulou School of Clinical Medicine Nanjing Medical University Nanjing 210008 Jiangsu Province China Department of Gastroenterology Nanjing Jiangbei People's Hospital Affiliated to Southeast University Medical School Nanjing 210048 Jiangsu Province China Nanjing University Medical School Nanjing 210093 Jiangsu Province China Department of Gastroenterology Nanjing Drum Tower Hospital the Affiliated Hospital of Nanjing University Medical School Nanjing 210008 Jiangsu Province China

AIM: To explore the preventive and therapeutic effects of Faecalibacterium prausnitzii(F. prausnitzii) supernatant on dextran sulfate sodium(DSS) induced colitis in ***: Forty C57BL/6J male mice were randomlydivided into four groups: control group, model group, treatment group, and prevention group. Mice were weighed daily. On day 10, the colon length was measured, the colorectal histopathologic damage score(HDS) was assessed, and plasma interleukin(IL)-17 A, IL-6, and IL-4 levels were detected by enzyme-linked immunosorbent assay. The expression of transcription factor retinoic acid-related orphan receptor-γt(RORγt) and IL-17 A in colon inflammatory mucosa tissue were determined by immunohistochemical assay, and the expression levels of RORγt m RNA, IL-17 A m RNA, and IL-6 m RNA were detected by real-time quantitative polymerase chain reaction(PCR). The proportion of Th17 in mononuclear cells in spleen was assayed by fluorescence activated cell sorter. RESULTS: When compared with the model group, the colon length(P < 0.05) and body weight(P < 0.01) in the treatment and prevention groups were significantly increased, and the colon HDS was decreased(P < 0.05 and P < 0.01). There was no statistical difference between the treatment group and prevention group. After treatment with F. prausnitzii supernatant, the plasma levels of IL-17 A and IL-6(P < 0.05), the protein and m RNA expression of IL-17 A and RORγt, and the Th17 cell ratio of spleen cells(P < 0.01) were significantly decreased compared to the model group. Plasma IL-4 level in the prevention group was significantly higher than that in the model group(P < 0.05), but there was no significant difference between these two groups in the expression of IL-6 in both the plasma and colon mucosa ***: F. prausnitzii supernatant exerts protective and therapeutic effects on DSS-induced colitis in mice, probably via inhibition of Th17 differentiation and IL- 17A secretion in the plasma and colon muco

关键词： Faecalibacterium prausnitzii Ulcerative colitis Animal model Th17 cell Treatment Prevention

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Comparative Evaluation of Antitumor Chinese Patent Medicine Injections Based on Evidence-Based Medicine and Markov Model

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World Journal of Traditional Chinese Medicine 2024年第2期10卷 220-229页

作者： Jing Yang Yan-Peng Wang Wen-gong yu yu-Yao Guan Prince Emmanuel Opoku Amponsah Xian-Shuai Tang Ying-Zi Sun Lei Zheng School of Medicine and Pharmacy Ocean University of ChinaQingdaoChina Department of Pharmacy Shandong Medical CollegeJinanChina Department of Pharmacy Cheeloo College of MedicineShandong Provincial Third HospitalShandong UniversityJinanChina Biomedical Engineering Graduate School University of ArizonaTucsonAZUSA

Objective: The objective of this study was to explore the safety, efficacy, economy, and other aspects of frequently-used antitumor Chinese patent medicine injections through a comprehensive evaluation of evidence-based medical research to provide scientific information and decision-making basis for drug selection, rational drug use, public awareness of safe drug use, and medical insurance. Materials and Methods: The names of five frequently used antitumor Chinese patent medicine injections were used as search terms, including the compound Sophora flavescens injection, Kangai injection, Aidi injection, Kanglaite injection, and Brucea javanica oil emulsion injection. Relevant evidence-based medical research was extracted from the databases. A descriptive evaluation was conducted, and the Markov model was used for the cost-benefit analysis. Finally, the above injections are ranked by comprehensive scores using the multi-criteria decision analysis and Delphi method. Results: The five injections had their own advantages in enhancing the effectiveness of anti-tumor treatments, improving the quality of life for cancer patients, and reducing the incidence of adverse reactions. From the perspective of economy and other attributes, compound S. flavescens was superior to B. javanica oil, Aidi, Kangai, and Kanglaite. After a comprehensive evaluation of the above five injections, the compound S. flavescens injection received the highest score. Conclusions: Compound S. flavescens, Kangai, Aidi, B. javanica oil, and Kanglaite have their own advantages in effectiveness and no significant difference in safety. Compound S. flavescens injection has the highest comprehensive score. Clinical attention should be paid to monitoring the initial stage of medication and special groups.

关键词： Anti-tumor Chinese patent medicine comprehensive evaluation medication safety pharmacoeconomics rational drug use

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Imidazole-Linked Fully Conjugated Covalent Organic Framework for High-Performance Sodium-Ion Battery

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CCS Chemistry 2024年第5期6卷 1255-1263页

作者： Lu Liu yu gong Yifan Tong Hao Tian Xubo Wang Yiming Hu Shaofeng Huang Weiwei Huang Sandeep Sharma Jingnan Cui Yinghua Jin Weitao gong Wei Zhang State Key Laboratory of Fine Chemicals School of Chemical EngineeringDalian University of TechnologyDalian 116024 Department of Chemistry University of Colorado BoulderBoulderColorado 80309 Hebei Key Laboratory of Applied Chemistry Yanshan UniversityQinhuangdao 066004

Covalent organic frameworks(COFs)have garnered increasing attention as promising electrode materials for sodium-ion batteries(SIBs)due to their ordered backbones,uniform pore sizes,and high surface ***,they also face challenges,including low capacity,unsatisfactory rate performance,and limited cycling stability,which pose the primary obstacles to their practical ***,a pyrenoimidazole-based COF,denoted as PyIm-COF,has been synthesized as a novel electrode material for highperformance SIBs using an efficient synthetic *** extended fully conjugated backbones and readily accessible redox-active sites,PyIm-COF demonstrates remarkable high-rate performance,delivering around 250 m Ah g^(−1)and excellent cycling stability,retaining 97.2%of its capacity even after 2500 cycles at 5 A g^(−1),which is higher than that of most previously reported COF-based *** work provides valuable insights into the development of nitrogen-rich conjugated COF electrode materials for rechargeable SIBs.

关键词： pyrenoimidazole fully conjugated backbones covalent organic framework electrode material sodium-ion battery

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Exploration of the mechanism by which icariin modulates hippocampal neurogenesis in a rat model of depression

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Neural Regeneration Research 2022年第3期17卷 632-642页

作者： Ning-Xi Zeng Hui-Zhen Li Han-Zhang Wang Kai-Ge Liu Xia-yu gong Wu-Long Luo Can Yan Li-Li Wu Research Center for Basic Integrative Medicine Guangzhou University of Chinese MedicineGuangzhouGuangdong ProvinceChina

Icariin(ICA) has a significant capacity to protect against depression and hippocampal injury,but it cannot effectively cross the bloodbrain barrier and accumulate in the ***,the mechanism by which ICA protects against hippocampal injury in depression remains *** this study,we performed proteomics analysis of cerebrospinal fluid to investigate the mechanism by which ICA prevents dysfunctional hippocampal neurogenesis in depression.A rat model of depression was established through exposure to chronic unpredictable mild stress for 6 weeks,after which 120 mg/kg ICA was administered subcutaneously every *** results showed that ICA alleviated depressive symptoms,learning and memory dysfunction,dysfunctional neurogenesis,and neuronal loss in the dentate gyrus of rats with *** stem cells from rat embryonic hippocampi were cultured in media containing 20% cerebrospinal fluid from each group of rats and then treated with 100 μM *** addition of cerebrospinal fluid from rats treated with ICA largely prevented the corticosterone-mediated inhibition of neuronal proliferation and ***-two differentially expressed proteins regulated by chronic unpredictable mild stress and ICA were identified through proteomics analysis of cerebrospinal *** proteins were mainly involved in the ribosome,PI3 K-Akt signaling,and interleukin-17 signaling *** reaction monitoring mass spectrometry showed that Rps4 x,Rps12,Rps14,Rps19,Hsp90 b1,and Hsp90 aa1 were up-regulated by chronic unpredictable mild stress and down-regulated by *** contrast,Htr A1 was down-regulated by chronic unpredictable mild stress and up-regulated by *** findings suggest that ICA can prevent depression and dysfunctional hippocampal neurogenesis through regulating the expression of certain proteins found in the cerebrospinal *** study was approved by the Experimental Animal Ethics Committee of Guangzhou University of Chinese

关键词： cerebrospinal fluid chronic unpredictable mild stress depression dysfunctional hippocampal neurogenesis hippocampus icariin proteomics ribosome pathway

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Fabrication of Novel Scaffolds Containing Collagen-I/Polylactic Acid/Nanohydroxyapatite via Co-electrospinning Methods

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Chemical Research in Chinese Universities 2010年第4期26卷 662-666页

作者： ZANG Jun-ting QI Xin LI Shu-qiang LI Dong-song gong yu-bao YANG Chen XU Feng LIU Jian-guo Department of Bone and Joint Surgery First Hospital of Jilin University Changchun 130021 P R. China

A novel scaffold containing collagen-I/polylactic acid（PLA）/nanohydroxyapatite（nHA） was prepared via co-electrospinning method. Different target substrates were used to improve the collection efficiency of this scaffold. The properties of the novel scaffold were compared with those of conventionally prepared ones. Compared to con- ventional method, the modified method was more efficient in producing the scaffold. Moreover, the porosity, thickness, and morphology of the novel scaffold were better than those of scaffolds prepared by conventional methods. The properties of collagen-I, collagen-I/PLA and collagen-I/PLA/nHA scaffolds were also compared. Diameters of the electrospun fibers ranged from 180 to 405 nm, and roughness was present on the surface of the fibers due to the deposition of crystals of nHA along the long axis of the fibers. The fibers of the collagen-I/PLA/nHA scaffold and the fibers of natural bone tissue had similar structure.

关键词： Electrospinning Collagen-I PLA Nanohydroxyapatite

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Shewanella oneidensis MR-1对地下水低渗透区中Cr(Ⅵ)和硝酸盐的协同异化还原

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中国环境科学 2024年第5期44卷 2473-2482页

作者：贡玉凤上海市环境科学研究院国家环境保护城市土壤污染控制与修复工程技术中心上海200233 同济大学环境科学与工程学院污染控制与资源化国家重点实验室上海200092

Cr(Ⅵ)和硝酸盐是地下水中常见的复合污染物,且有可能通过迁移扩散至低渗透区而导致其修复问题被忽视.利用Shewanella oneidensis MR-1作为功能微生物,分别研究了摇瓶和非均质模拟装置中两种污染物的协同异化还原过程.结果表明:摇瓶实验... 详细信息

Cr(Ⅵ)和硝酸盐是地下水中常见的复合污染物,且有可能通过迁移扩散至低渗透区而导致其修复问题被忽视.利用Shewanella oneidensis MR-1作为功能微生物,分别研究了摇瓶和非均质模拟装置中两种污染物的协同异化还原过程.结果表明:摇瓶实验中,Cr (Ⅵ)的去除主要依赖于初始菌浓度,而受硝酸盐浓度、电子供体乳酸盐浓度的影响较小.反之,硝酸盐的还原则明显受到Cr (Ⅵ)的抑制,当Cr(Ⅵ)浓度在0到0.02mmol/L时,其一级动力学还原速率从0.635h^(-1)降至0.164h^(-1).同时,乳酸盐氧化也明显受到Cr(Ⅵ)的抑制,表明Shewanella oneidensis MR-1的代谢过程可能受到了Cr(Ⅵ)的毒性干扰.在非均相装置实验中,Cr(Ⅵ)进水浓度小于等于5mg/L(约0.1mmol/L)时,15d基本可以完全去除,对应硝酸盐去除60%左右;当Cr(Ⅵ)浓度增至10mg/L (约0.2mmol/L)时,Cr(Ⅵ)和硝酸盐的去除率分别降至40%和50%左右.装置内截留的Cr(Ⅵ)还原产物以Cr(OH)_3为主,硝酸盐的还原产物以氨为主.

关键词： Shewanella oneidensis MR-1 六价铬硝酸盐

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Biosafety of a 3D-printed intraocular lens made of a poly (acrylamide-co-sodium acrylate) hydrogel in vitro and in vivo

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International Journal of Ophthalmology(English edition) 2020年第10期13卷 1521-1530页

作者： Jia-Wen Li Yi-Jian Li Xi-Su Hu yu gong Ben-Bin Xu Hai-Wei Xu Zheng-Qin Yin Southwest Hospital/Southwest Eye Hospital Third Military Medical University(Army Medical University)Chongqing 400038China Key Lab of Visual Damage and Regeneration&Restoration of Chongqing Chongqing 400038China Smart Materials and Surfaces Lab Faculty of Engineering and EnvironmentNorthumbria UniversityNewcastle upon Tyne NE18STUK

AIM:To assess the biosafety of a poly(acrylamide-cosodium acrylate)hydrogel(PAH)as a 3D-printed intraocular lens(IOL)***:The biosafety of PAH was first evaluated in vitro using human lens epithelial cells(LECs)and the ARPE19 cell line,and a cell counting kit-8(CCK-8)assay was performed to investigate alterations in cell proliferation.A thin film of PAH and a conventional IOL were intraocularly implanted into the eyes of New Zealand white rabbits respectively,and a sham surgery served as control *** anterior segment photographs,intraocular pressure(IOP),blood parameters and electroretinograms(ERG)were *** cytokines in the aqueous humor,such as TNFαand IL-8,were examined by *** apoptosis of the retina was investigated by TUNEL assay,and macro PAHge activation was detected by ***:PAH did not slow cell proliferation when cocultured with human LECs or ARPE19 *** implantation of a thin film of a 3 D-printed IOL composed of PAH did not affect the IOP,blood parameters,ERG or optical structure in any of the three experimental groups(n=3 for each).Both TNFαand IL-8 in the aqueous humor of PAH group were transiently elevated 1 wk post-operation and recovered to normal levels at 1 and 3 mo ***1+macroPAHges in the anterior chamber angle in PAH group were increased markedly compared to those of the control group;however,there was no significant difference compared to those in the IOL ***:PAH is a safe material for 3D printing of personal IOLs that hold great potential for future clinical applications.

关键词： cataract biosafety lens epithelial cells ARPE19 cells intraocular lens 3D printing poly hydrogel rabbit

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From Society to Solitude:An In-depth Analysis of Thoreau’s Characters in Walden

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Journal of Literature and Art Studies 2024年第10期14卷 898-903页

作者： LU Xiao-jun gong yu-miao School of Foreign Languages Jiangsu Ocean UniversityLianyungangChina

With the deterioration of the environment and the unbalanced relationship between man and nature,people are plagued by varieties of social problems and rising spiritual *** paper aims to analyze Thoreau’s characters in Walden and attempts to provide the implications for our contemporary *** with Thoreau’s life experience and the background of Walden,the paper starts with the analysis of Thoreau’s characters from three aspects concerning his pursuit of inner serenity,search for true essence of life and abiding passion for life,which constitute his unique and independent characters and provide the enlightenment for contemporary *** concluded that unique thoughts,attitudes,and spirit embedded in Thoreau’s characters are inextricably connected with our contemporary *** personal experience and practice of solitary life at Walden Pond is a testimony to the necessity of closing to nature actively and confronting life passionately.

关键词： Thoreau Walden solitude characters enlightenment for life

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