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Time-course pattern of neuronal loss and gliosis in gerbil hippocampi following mild, severe, or lethal transient global cerebral ischemia

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Neural Regeneration Research 2019年第8期14卷 1394-1403页

作者： Tae-Kyeong Lee Hyunjung kim Minah Song Jae-Chul Lee Joon Ha Park Ji Hyeon Ahn Go Eun Yang Hyeyoung kim Taek Geun Ohk Myoung Cheol Shin Jun Hwi Cho Moo-Ho Won Depart merit of Neurobiology School of Medici ne Kangw on National University Chuncheon Gangwon Republic of Korea Department of Biomedical Science and Research Institute for Bioscience and Biotechnology Hallym University Chuncheon Gangwon Republic of Korea Department of Radiology Kangwon National University Hospital Chuncheon Gangxvon Republic of Korea Department of Anesthesiology and Pain Medicine Chungju Hospital Konkuk University School of Medicine Chungju Chungcheongbuk Republic of Korea Department of Emergency Medicine School of Medicine Kangwon National University Chuncheon Gangwon Republic of Korea

Transient ischemia in the whole brain leads to neuronal loss/death in vulnerable brain regions. The striatum, neocortex and hippocampus selectively loose specific neurons after transient ischemia. Just 5 minutes of transient ischemia can cause pyramidal neuronal death in the hippocampal cornu ammonis (CA) 1 field at 4 days after transient ischemia. In this study, we investigated the effects of 5-minute (mild), 15-minute (severe), and 20-minute (lethal) transient ischemia by bilateral common carotid artery occlusion (BCCAO) on behavioral change and neuronal death and gliosis (astrocytosis and microgliosis) in gerbil hippocampal subregions (CA1-3 region and dentate gyrus). We performed spontaneous motor activity test to evaluate gerbil locomotor activity, cresyl violet staining to detect cellular distribution, neuronal nuclei immunohistochemistry to detect neuronal distribution, and Fluoro-Jade B histofluorescence to evaluate neuronal death. We also conducted immunohistochemical staining for glial fibrillary acidic protein and ionized calcium-binding adapter molecule 1 (Ibal) to evaluate astrocytosis and microgliosis, respectively. Animals subjected to 20-minute BCCAO died in at least 2 days. BCCAO for 15 minutes led to pyramidal cell death in hippocampal CA1-3 region 2 days later and granule cell death in hippocampal de匚tate gyrus 5 days later. Similar results were not found in animals subjected to 5-minute BCCAO. Gliosis was much more rapidly and severely progressed in animals subjected to 15-minute BCCAO than in those subjected to 5- minute BCCAO. Our results indicate that neuronal loss in the hippocampal formation following transient ischemia is significantly different according to regions and severity of transient ischemia. The experimental protocol was approved by Institutional Animal Care and Use Committee (AICUC) of Kangwon National University (approval No. KW-180124-1) on May 22, 2018.

关键词： transient global brain ischemia delayed neuronal death glial activation ischemic duration hippocampus spontaneous motor activity Mongolian gerbil histology neural regeneration

Conservation and Diversification of the SHR-SCR- SCL23 Regulatory Network in the Development of the Functional Endodermis in Arabidopsis Shoots

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Molecular Plant 2016年第8期9卷 1197-1209页

作者： Eun Kyung Yoon Souvik Dhar Mi-Hyun Lee Jae Hyo Song Shin Ae Lee Gyuree kim Sejeong Jang Ji Won Choi Jeong-Eun Choe Jeong Hoe kim Myeong Min Lee Jun Lim Department of Systems Biotechnology Konkuk University Seoul 05029 Korea Department of Systems Biology Yonsei University Seoul 03722 Korea Department of Biology Kyungpook National University Daegu 41566 Korea 4TheSe tlRors cotribCited equally/to ttats article Present address: Department of Agricultural Biotechnology National Institute of Agricultural Sciences Jeonju 54874 Korea Present address: Department of Agricultural Biology National Institute of Agricultural Sciences Wanju 55365 Korea

Development of the functional endodermis of Arabidopsis thaliana roots is controlled, in part, by GRAS transcription factors, namely SHORT-ROOT （SHR）, SCARECROW （SCR）, and SCARECROW-LIKE 23 （SCL23）. Recently, it has been shown that the SHR-SCR-SCL23 regulatory module is also essential for spec- ification of the endodermis （known as the bundle sheath） in leaves. Nevertheless, compared with what is known about the role of the SHR-SCR-SCL23 regulatory network in roots, the molecular interactions of SHR, SCR, and SCL23 are much less understood in shoots. Here, we show that SHR forms protein com- plexes with SCL23 to regulate transcription of SCL23 in shoots, similar to the regulation mode of SCR expression. Our results indicate that SHR acts as master regulator to directly activate the expression of SCR and SCL23. In the SHR-SCR-SCL23 network, we found a previously uncharacterized negative feed- back loop whereby SCL23 modulates SHR levels. Through molecular, genetic, physiological, and morpho- logical analyses, we also reveal that the SHR-SCR-SCL23 module plays a key role in the formation of the endodermis （known as the starch sheath） in hypocotyls. Taken together, our results provide new insights into the regulatory role of the SHR-SCR-SCL23 network in the endodermis development in both roots and shoots.

关键词： endodermis gene regulatory network SHORT-ROOT SCARECROW SCARECROW-LIKE 23,transcription factor

High‑Energy-Density Fiber Supercapacitors Based on Transition Metal Oxide Nanoribbon Yarns for Comprehensive Wearable Electronics

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Advanced Fiber Materials 2024年第6期6卷 1927-1941页

作者： Junseong Ahn Suchithra Padmajan Sasikala Yongrok Jeong Jin Goo kim Ji‑Hwan Ha Soon Hyoung Hwang Sohee Jeon Junhyuk Choi Byung‑Ho Kang Jihyeon Ahn Jun‑Ho Jeong Sang Ouk kim Inkyu Park Department of Electro‑Mechanical Systems Engineering Korea UniversitySejong 30019Republic of Korea Department of Materials Science and Engineering Korea Advanced Institute of Science and Technology(KAIST)Daejeon 34141Republic of Korea National Creative Research Initiative Center for Multi‑Dimensional Directed Nanoscale Assembly Korea Advanced Institute of Science and Technology(KAIST)Daejeon 34141Republic of Korea Department of Mechanical Engineering Korea Advanced Institute of Science and Technology(KAIST)Daejeon 34141Republic of Korea Department of Nano Manufacturing Technology Korea Institute of Machinery and Materials(KIMM)Daejeon 34103Republic of Korea Radioisotope Research Division Korea Atomic Energy Research Institute(KAERI)Daejeon 34057Republic of Korea

Fiber supercapacitors(FSs)based on transition metal oxides(TMOs)have garnered considerable attention as energy stor-age solutions for wearable electronics owing to their exceptional characteristics,including superior comfortability and low *** materials are known to exhibit high energy densities,high specific capacitances,and fast redox ***,current fabrication methods for these structures primarily rely on chemical deposition,often resulting in undesir-able material structures and necessitating the use of additives,which can degrade the electrochemical performance of such ***,physically deposited TMO nanoribbon yarns generated via delamination engineering of nanopatterned TMO/metal/TMO trilayer arrays are proposed as potential high-performance *** prepare these arrays,the target materials were initially deposited using a nanoline mold,and subsequently,the nanoribbon was suspended through selective plasma etching to obtain the desired twisted yarn *** of the direct formation of TMOs on Ni electrodes,a high energy/power density and excellent electrochemical stability were achieved in asymmetric FS devices incorporating CoNixOy nanoribbon yarns and graphene ***,a triboelectric nanogenerator,pressure sensor,and flexible light-emitting diode were synergistically combined with the *** integration of wearable electronic components,encompassing energy harvesting,energy storage,and powering sensing/display devices,is promising for the development of future smart textiles.

关键词： Fiber supercapacitor Transition metal oxide Nanoribbon yarn Wearable devices Nanostructuring

Zn-doped nickel iron(oxy)hydroxide nanocubes passivated by polyanions with high catalytic activity and corrosion resistance for seawater oxidation

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Journal of Energy Chemistry 2023年第6期81卷 82-92,I0004页

作者： So Jung kim Heechae Choi Jeong Ho Ryu Kang Min kim Sungwook Mhin Arpan Kumar Nayak Junghwan Bang Minyeong Je Ghulam Ali Kyung Yoon Chung Kyeong-Han Na Won-Youl Choi Sunghwan Yeo Jin Uk Jang HyukSu Han Department of Energy Engineering Konkuk University120 Neungdong-roGwangjin-guSeoul 05029Republic of Korea Theoretical Materials&Chemistry Group Institute of Inorganic ChemistryUniversity of CologneGreinst.650939CologneGermany Materials Lab Geobuk-ro 25-50Incheon 22793Republic of Korea Department of Materials Science and Engineering Korea National University of Transportation50 Daehak-roChungjuChungbuk 27469Republic of Korea Korea Institute of Industrial Technology 137-41 Gwahakdanji-roGangneung-siGangwon 25440Republic of Korea Department of Advanced Materials Engineering Kyonggi UniversitySuwon 16227Republic of Korea Korea Institute of Industrial Technology 156 Gaetbeol-roYeonsu-guIncheon 406-840Republic of Korea U.S.-Pakistan Center for Advanced Studies in Energy(USPCASE) National University of Sciences and Technology(NUST)H-12IslamabadPakistan Energy Storage Research Center Korea Institute of Science and TechnologyHwarang-ro 14-gil 5Seongbuk-guSeoul 02792Republic of Korea Division of Energy and Environment Technology KIST SchoolKorea University of Science and TechnologySeoul 02792Republic of Korea Department of Metal and Materials Engineering Gangneung-Wonju National University 7 JukheongilGangneungGangwon 25457Republic of Korea Smart Hydrogen Energy Center Gangneung-Wonju National University7 JukheongilGangneungGangwon 25457Republic of Korea Innovative Fuel Development Division Korea Atomic Energy Research InstituteDaedeok-daero 989-111Yuseong-guDaejeon 305-353Republic of Korea

Electrochemical water splitting to produce hydrogen fuel is a promising renewable energy-conversion ***-scale electrolysis of freshwater may deplete water resources and cause water scarcity ***,seawater electrolysis is a potential solution to the future energy and water *** seawater electrolysis,it is critical to develop cost-effective electrocatalysts to split seawater without chloride ***,we present zinc-doped nickel iron(oxy)hydroxide nanocubes passivated by negatively charged polyanions(NFZ-PBA-S)that exhibits outstanding catalytic activity,stability,and selectivity for seawater *** dopants and polyanion-rich passivated surface layers in NFZ-PBA-S could effectively repel chlorine ions and enhance corrosion resistance,enabling its excellent catalytic activity and stability for seawater oxidation.

关键词： Seawater splitting Oxygen evolution reaction Electrocatalyst Layered double hydroxide Sulfidation

Evaluation of microstructure and mechanical properties of Mg-xCe-0.5Zn alloys by nano-indentation

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Transactions of Nonferrous Metals Society of China 2010年第7期20卷 1240-1243页

作者： K.C.PARK B.H.kim Y.H.PARK I.M.PARK Department of Material Science and Engineering Pusan National UniversitySan 30 Jangjeon-dongGeumjeong-guBusan 609-735Korea

The microstructure and mechanical properties of Mg-xCe-0.5Zn （x=0.5, 1.5, 2.5, molar fraction, %） alloys were examined using a nano-indentation technique. The alloys were fabricated using a vacuum induction melting method under an argon atmosphere The microstruetures of Mg-xCe-0.5Zn alloys mainly consist ofa-Mg and eutectic Mg12Ce phase. The volume fraction and size of the eutectic Mgl2Ce phase increase with increasing Ce contents. Nano-indentation test results show that the indentation hardness and elastic modulus of the eutectic Mg12Ce phase are higher than those of the a-Mg matrix. In addition, the mean indentation hardness and elastic modulus of the Mg-xCe-0.5Zn alloys increase with the Ce addition amount increasing.

关键词： Mg alloys Mg-Ce-Zn rare earth microstructure mechanical properties nano-indentation

An in vivo Evaluation of Ultra-fine Grained Titanium Implants

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Journal of Materials Science & Technology 2009年第4期25卷 556-560页

作者： S. Bindu K.P. Sanosh K. Smetana A. Balakrishnan T.N. kim Department of Life Science and Genetic Engineering Paichai University Department of Information and Electronic Materials Engineering Paichai University Charles University 1st and 2nd Faculty of MedicineInstitute of Anatomy and Center of Cell Therapy and Tissue Repair

The present work focuses mainly on an in vivo evaluation of ultra fine grained titanium （UFG-Ti） obtained by severe plastic deformation （SPD）. The SPD on commercially produced Grade 2 titanium （Cp-Ti） resulted in the refinement of the grain size by several orders of magnitude. Polished surfaces having similar roughness from both UFG-Ti and Cp-Ti were prepared. In vitro test revealed the presence of fibronectin, which was involved in the attachment of the cells to the substrate. Phase contrast micrographs showed the highest signal of fibronectin in UFG-Ti, indicating that it is more cytocompatible than Cp-Ti. In vivo tests, by subcutaneous implantation of the metals in the rats showed the better biocompatibility of UFG-Ti over Cp-Ti. The improved biocompatibility of UFG-Ti was attributed to the presence of surface discontinuities （in the form of nano-defects）, surface energy, higher wettability, surface stress and stable TiO2 films, which increased the protein adsorption on the surface.

关键词： Surfaces Grain size Titanium In vivo Biocompatibility

Behavior of CaO and Calcium in pure Magnesium

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Rare Metals 2006年第z2期25卷 150-154页

作者： HA Seong-Ho LEE Jin-Kyu JO Hyung-Ho JUNG Seung-Boo kim Shae K. Department of Advanced Material Engineering Sungkyunkwan University 300 Chunchun-dong Jangan-gu Suwon Gyunggi-do 440-746 KoreaAdvanced Material Division Korea Institute of Industrial Technology 7-47 Songdo-dong Yeonsu-gu Incheon 406-840 Korea Advanced Material Division Korea Institute of Industrial Technology 7-47 Songdo-dong Yeonsu-gu Incheon 406-840 Korea Department of Advanced Material Engineering Sungkyunkwan University 300 Chunchun-dong Jangan-gu Suwon Gyunggi-do 440-746 Korea

Mg alloys exhibit a number of good properties such as low density, good castability and high specific strength. However, molten Mg and Mg alloys are ignited without the melt protective gases during melting and casting process due to their high reactivity. The purpose of this study is to investigate effects of Ca and CaO on pure Mg through microstructure observation, ignition test and phase analysis. With increasing Ca and CaO contents, the ignition resistance of Ca or CaO added pure Mg is increased and the grains are refined. As results of XRD and EDS, CaO is reduced to Ca in CaO added pure Mg. Mg2Ca phase is formed even in 0.1 wt.%CaO added pure Mg by reduction mechanism, while Mg2Ca phase is formed over 1.35 wt.% Ca added pure Mg.

关键词： Mg CaO ignition resistance Mg2Ca phase

Superresolution structured illumination microscopy reconstruction algorithms:a review

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Light(Science & Applications) 2023年第8期12卷 1510-1543页

作者： Xin Chen Suyi Zhong Yiwei Hou Ruijie Cao Wenyi Wang Dong Li Qionghai Dai Donghyun kim Peng Xi Department of Biomedical Engineering College of Future TechnologyPeking UniversityBeijing 100871China National Biomedical Imaging Center Peking UniversityBeijing 100871China National Laboratory of Biomacromolecules CAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijingChina Department of Automation Tsinghua UniversityBeijingChina Institute for Brain and Cognitive Sciences Tsinghua UniversityBeijingChina Beijing Key Laboratory of Multidimension&Multiscale Computational Photography Tsinghua UniversityBeijingChina Beijing Laboratory of Brain and Cognitive Intelligence Beijing Municipal Education CommissionBeijingChina School of Electrical and Electronic Engineering Yonsei University50 Yonsei-RoSeodaemun-GuSeoul 03722Korea

Structured illumination microscopy(SIM)has become the standard for next-generation wide-field microscopy,offering ultrahigh imaging speed,superresolution,a large fiield-of-view,and long-term *** the past decade,SIM hardware and software have flourished,leading to successful applications in various biological ***,unlocking the full potential of SIM system hardware requires the development of advanced reconstruction ***,we introduce the basic theory of two SIM algorithms,namely,optical sectioning SIM(OS-SIM)and superresolution SIM(SR-SIM),and summarize their implementation *** then provide a brief overview of existing OS-SIM processing algorithms and review the development of SR-SIM reconstruction algorithms,focusing primarily on 2D-SIM,3D-SIM,and *** showcase the state-of-the-art development of SIM systems and assist users in selecting a commercial SIM system for a specific application,we compare the features of representative off-the-shelf SIM ***,we provide perspectives on the potential future developments of SIM.

关键词： illumination hardware offering

Micromagnetics and Signal Analysis of Shielded Spin-Valve Multilayer

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Chinese Physics Letters 1996年第6期13卷 469-472页

作者： ZHANG Huai-wu YANG Shi-qing LIU Ying-li WANG Hao-cai H.J.kim Institute of Materials and Information Engineering University of Electronic Science and TechnologyChengdu 610054 Magnetic Alloy Laboratory Metals DivisionKorea Institute of Science and TechnologyP.O.Box 131ChenogryangSeoulKorea

In order to linearize a spin-valve giant magnetoresistance head,the exchanges layer sputtered at fringe area or a permanent magnet layer longitudinal biasing for domain stabilization based on a physical calculated model may be *** correlations between the head performance characteristic and head geometric parameters have been simulated by putting the head on a suitable front-end electric *** simulative results show that the optimal sensing current is from 10 to 40mA and the linear geometric parameteres for gap width are at the range of G_(1)+G_(2)≤0.5μm.

关键词： stabilization permanent Signal