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Acoustofluidic separation of prolate and spherical micro-objects

Microsystems & Nanoengineering 2024年第1期10卷 217-230页

作者： Muhammad Soban Khan Mushtaq Ali Song Ha Lee Keun young jang Seong Jae Lee Jinsoo Park Department of Mechanical Engineering Chonnam National University77 Yongbong-roBuk-guGwangju 61186Republic of Korea Department of Polymer Engineering The University of Suwon17 Wauan-gilBongdam-eupHwaseongGyeonggi 18323Republic of Korea

Most microfluidic separation techniques rely largely on object size as a separation *** ability to separate micro-objects based on their shape is crucial in various biomedical and chemical ***,we develop an ondemand,label-free acoustofluidic method to separate prolate ellipsoids from spherical microparticles based on traveling surface acoustic wave-induced acoustic radiation force and *** freely rotating non-spherical microobjects were aligned under the progressive acoustic field by the counterrotating radiation torque,and the major axis of the prolate ellipsoids was parallel to the progressive wave *** specific alignment of the ellipsoidal particles resulted in a reduction in the cross-sectional area perpendicular to the wave *** a consequence,the acoustic backscattering decreased,resulting in a decreased magnitude of the radiation *** the variation in radiation force,which depended on the micro-object morphology enabled the acoustofluidic shape-based *** conducted numerical simulations for the wave scattering of spherical and prolate objects to elucidate the working mechanism underlying the proposed method.A series of experiments with polystyrene microspheres,prolate ellipsoids,and peanut-shaped microparticles were performed for *** quantitative analysis of the separation efficiency,we confirmed the high purity and high recovery rate of the proposed acoustofluidic shapebased separation of *** a bioparticle,we utilize Thalassiosira eccentrica to perform shape-based separation,as the species has a variety of potential applications in drug delivery,biosensing,nanofabrication,bioencapsulation and immunoisolation.

关键词： separation spherical rotating

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Retinoic acid enhances lactoferrin-induced IgA responses by increasing betaglycan expression

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Cellular & Molecular Immunology 2016年第6期13卷 862-870页

作者： Jeong-Min Lee young-saeng jang Bo-Ra Jin Sun-Jin Kim Hyeon-Jin Kim Bo-Eun Kwon Hyun-Jeong Ko Sung-il Yoon Geun-Shik Lee Woan-Sub Kim Goo-young Seo Pyeung-Hyeun Kim Department of Molecular Bioscience School of Biomedical Science Kangwon National University Chuncheon Republic of Korea Laboratory of Microbiology and Immunology College of Pharmacy Kangwon National University Chuncheon Republic of Korea Department of Systems Immunology College of Biomedical Science Kangwon National University Chuncheon 200-701 Republic of Korea College of Veterinary Medicine Kangwon National University Chuncheon Gangwon 200-701 Republic of Korea Department of Animal Life and Environmental Science College of Agriculture and Life Science Hankyong National University Anseong-si 456-749 Republic of Korea

Lactoferrin （LF） and retinoic acid （RA） are enriched in colostrum, milk, and mucosal tissues. We recently showed that LF-induced IgA class switching through binding to betaglycan （transforming growth factor-beta receptor III, TpRIII） and activation of canonical TGF-p signaling. We investigated the combined effect of LF and RA on the overall IgA response. An increase in IgA production by LF was further augmented by RA. This combination effect was also evident in Ig germ-line α （GLa） transcription and GLa promoter activity, indicating that LF in cooperation with RA increased IgA isotype switching. We subsequently found that RA enhanced TβRIII expression and that this increase contributed to LF-stimulated IgA production. In addition to the IgA response, LF and RA in combination also enhanced the expression of the gut-homing molecules C-C chemokine receptor 9 （CCR9） and a4β7 on B cells. Finally, peroral administration of LF and RA enhanced the frequency of CCR9＋ IgA＋ plasma cells in the lamina propria. Taken together, these results suggest that LF in cooperation with RA can contribute to the establishment of gut IgA responses.

关键词： gut homing molecule IgA lactoferrin retinoic acid TGF-β receptor

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Programmable directional color dynamics using plasmonics

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Microsystems & Nanoengineering 2024年第1期10卷 325-334页

作者： Gyurin Kim Doeun Kim Soeun Ko jang-Hwan Han Juhwan Kim Joo Hwan Ko young Min Song Hyeon-Ho Jeong School of Electrical Engineering and Computer Science Gwangju Institute of Science and TechnologyGwangju 61005Republic of Korea Department of Semiconductor Engineering Gwangju Institute of Science and TechnologyGwangju 61005Republic of Korea Artificial Intelligence(AI)Graduate School Gwangju Institute of Science and TechnologyGwangju 61005Republic of Korea

Adaptive multicolor filters have emerged as key components for ensuring color accuracy and resolution in outdoor visual ***,the current state of this technology is still in its infancy and largely reliant on liquid crystal devices that require high voltage and bulky structural ***,we present a multicolor nanofilter consisting of multilayered‘active’plasmonic nanocomposites,wherein metallic nanoparticles are embedded within a conductive polymer *** nanocomposites are fabricated with a total thickness below 100 nm using a‘lithography-free’method at the wafer level,and they inherently exhibit three prominent optical modes,accompanying scattering phenomena that produce distinct dichroic reflection and transmission ***,a pivotal achievement is that all these colors are electrically manipulated with an applied external voltage of less than 1 V with 3.5 s of switching speed,encompassing the entire visible ***,this electrically programmable multicolor function enables the effective and dynamic modulation of the color temperature of white light across the warm-to-cool spectrum(3250 K-6250 K).This transformative capability is exceptionally valuable for enhancing the performance of outdoor optical devices that are independent of factors such as the sun’s elevation and prevailing weather conditions.

关键词： directional prevailing exceptional

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Unification of Gravitational and Strong Interaction Fields Using Partial Gauge Symmetry

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Journal of High Energy Physics, Gravitation and Cosmology 2024年第2期10卷 515-533页

作者： young Hwan Yun Kiho jang Zero Theoretical Physics Lab Seoul Republic of Korea

We propose the new field potential by maintaining both the symmetry of the scalar gauge and the conservation law keeping N?ether’s theorem, while disregarding the symmetry of the vector gauge. The new potential forms like the well-type potential where a particle behaves almost freely but is very hard to escape without external energy, which can be interpreted as local confinement and asymptotic freedom. By assuming a 2-dimensional metric tensor in 4-dimensional space-time, we suggest the existence of 3 kinds of particles that resemble QCD with 3 color charges. We also show that the mass term exists but comes to zero and derive the charge and spin values. We can regard the particle with this new potential as a gluon, and the interaction in this well-type potential as a strong interaction for the properties of mass, charge, spin, and its behavior. We suggest the eight-fold way with this new particle, which is similar to the existing method based on SU (3) symmetry. Even though the strong interaction has been analyzed in the standard model and string theory, we build a new consistent model based on the theory of relativity including Riemann geometry, and show the unification of gravitational and strong interactional field.

关键词： Strong Interaction Gauge Symmetry Relativity QCD Confinement Asymptotic Freedom

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Harnessing SARS-CoV-2-specific CD8^(+)T cells to kill target tumor cells for cancer immunotherapy

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Cancer Communications 2024年第1期44卷 173-177页

作者： Keunok Jung Deok-Han Ko Jeong-Yun jang young Rong Kim Jung Yeon Heo Yong-Sung Kim Department of Allergy and Clinical Immunology Ajou University School of MedicineSuwonRepublic of Korea Department of Molecular Science and Technology Ajou UniversitySuwonRepublic of Korea Department of Infectious Diseases Ajou University School of MedicineSuwonRepublic of Korea

Dear Editor,During the coronavirus disease 2019(COVID-19)pan-demic era,many individuals were naturally infected with or vaccinated against severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),resulting in the p... 详细信息

关键词： acute respiratory immunotherapy

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Generation of double knockout cattle via CRISPR-Cas9 ribonucleoprotein(RNP)electroporation

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Journal of Animal Science and Biotechnology 2024年第1期15卷 456-462页

作者： Gyeong-Min Gim Kyeong-Hyeon Eom Dong-Hyeok Kwon Dae-Jin Jung Dae-Hyun Kim Jun-Koo Yi Jae-Jung Ha Ji-Hyun Lee Seong-Beom Lee Woo-Jae Son Soo-young Yum Won-Wu Lee Goo jang Laboratory of Theriogenology and Biotechnology Department of Veterinary Clinical ScienceCollege of Veterinary Medicine and the Research Institute of Veterinary ScienceSeoul National UniversitySeoulRepublic of Korea LARTBio Co. Ltd.SeoulRepublic of Korea Gyeongsangbukdo Livestock Research Institute YeongjuRepublic of Korea Comparative Medicine Disease Research Center Seoul National UniversitySeoulRepublic of Korea

Background Genome editing has been considered as powerful tool in agricultural ***,genome editing progress in cattle has not been fast as in other mammal species,for some disadvantages including long gestational periods,single pregnancy,and high raising ***,technically demanding methods such as microinjection and somatic cell nuclear transfer(SCNT)are needed for gene editing in *** this point of view,electroporation in embryos has been risen as an *** First,editing efficiency of our electroporation methods were tested for *** of mutation on embryo was confirmed by T7E1 *** first combination,mutation rates for MSTN and PRNP were 57.6%±13.7%and 54.6%±13.5%,*** case of MSTN/BLG,mutation rates were 83.9%±23.6%for MSTN,84.5%±18.0%for ***,the double-KO embryos were transferred to surrogates and mutation rate was identified in resultant calves by targeted deep *** recipients were transferred for MSTN/PRNP,4 calves were delivered,and one calf underwent an induction for double *** surrogates were given double-KO embryos for MSTN/BLG,and four of the six calves that were born had mutations in both *** These data demonstrated that production of genome edited cattle via electroporation of RNP could be effectively ***,MSTN and PRNP from beef cattle and MSTN and BLG from dairy cattle have been born and they will be valuable resources for future precision breeding.

关键词： Beta-lactoglobulin Cattle CRISPR-Cas9 Electroporation Knockout MSTN PRNP

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Dimensionality-driven metal to Mott insulator transition in two-dimensional 1T-TaSe2

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National Science Review 2024年第3期11卷 108-118页

作者： Ning Tian Zhe Huang Bo Gyu jang Shuaifei Guo Ya-Jun Yan Jingjing Gao Yijun Yu Jinwoong Hwang Cenyao Tang Meixiao Wang Xuan Luo Yu Ping Sun Zhongkai Liu Dong-Lai Feng Xianhui Chen Sung-Kwan Mo Minjae Kim young-Woo Son Dawei Shen Wei Ruan Yuanbo Zhang State Key Laboratory of Surface Physics New Cornerstone Science Laboratory and Department of PhysicsFudan University Shanghai Qi Zhi Institute Shanghai Research Center for Quantum Sciences Institute for Nanoelectronic Devices and Quantum Computing Fudan University Zhangjiang Fudan International Innovation Center Fudan University State Key Laboratory of Functional Materials for Informatics Shanghai Institute of Microsystem and Information Technology(SIMIT)Chinese Academy of Sciences School of Physical Science and Technology ShanghaiTech University Korea Institute for Advanced Study School of Emerging Technology and Department of Physics University of Science and Technology of China Advanced Light Source Lawrence Berkeley National Laboratory ShanghaiTech Laboratory for Topological Physics Key Laboratory of Materials Physics Institute of Solid State Physics Hefei Institutes of Physical ScienceChinese Academy of Sciences High Magnetic Field Laboratory Hefei Institutes of Physical Science Chinese Academy of Sciences Collaborative Innovation Centre of Advanced Microstructures Nanjing University Department of Physics University of Science and Technology of China and Key Laboratory of Strongly Coupled Quantum Matter Physics Chinese Academy of Sciences National Synchrotron Radiation Laboratory University of Science and Technology of China

Two-dimensional materials represent a major frontier for research into exotic many-body quantum phenomena. In the extreme two-dimensional limit, electron-electron interaction often dominates over other electronic energy scales, leading to strongly correlated effects such as quantum spin liquid and unconventional *** dominance is conventionally attributed to the lack of electron screening in the third dimension. Here, we discover an intriguing metal to Mott insulator transition in 1T-TaSe2that defies conventional wisdom. Specifically, we find that dimensionality crossover, instead of reduced screening, drives the transition in atomically thin 1T-TaSe2. A dispersive band crossing the Fermi level is found to be responsible for the bulk metallicity in the material. Reducing the dimensionality,however, effectively quenches the kinetic energy of these initially itinerant electrons, and drives the material into a Mott insulating *** dimensionality-driven metal to Mott insulator transition resolves the long-standing dichotomy between metallic bulk and insulating surface of 1T-TaSe2. Our work further reveals a new pathway for modulating two-dimensional materials that enables exploring strongly correlated systems across uncharted parameter space.

关键词： two-dimensional materials 1T-TaSe2 dimensionality crossover metal to Mott insulator transition

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Functionally graded structure of a nitride-strengthened Mg_(2)Si-based hybrid composite

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Journal of Magnesium and Alloys 2024年第3期12卷 1239-1256页

作者： Jeongho Yang Woongbeom Heogh Hogi Ju Sukhyun Kang Tae-Sik jang Hyun-Do Jung Mohammad Jahazi Seung Chul Han Seong Je Park Hyoung Seop Kim Susmita Bose Amit Bandyopadhyay Martin Byung-Guk Jun young Won Kim Dae-kyeom Kim Rigoberto CAdvincula Clodualdo Aranas Jr Sang Hoon Kim School of Mechanical Engineering Pusan National UniversityBusan 46241Republic of Korea Department of Mechanical Design Engineering Hanyang UniversitySeoul 04763Republic of Korea Process Research 3 Team LG Display Co.Ltd.PajuGyeonggi-do 10845Republic of Korea Department of Materials Science and Engineering Chosun UniversityGwangju 61452Republic of Korea Institute of Well-Aging Medicare&Chosun University LAMP Center Chosun UniversityGwangju 61452Republic of Korea Division of Materials Science and Engineering Hanyang UniversitySeoul 04763Republic of Korea Department of Mechanical Engineering École de Technologie SupérieureMontrealQuebec H3C 1K3Canada Material&Component Convergence R&D Center Korea Construction Equipment Technology InstituteGunsanJeollabuk-do 54002Republic of Korea School of Mechanical and Aerospace Engineering Nanyang Technological UniversitySingapore 639798Singapore Graduate Institute of Ferrous&Eco Materials Technology Pohang University of Science and TechnologyPohangGyeongsangbuk-do 37673Republic of Korea Advanced Institute for Materials Research Tohoku UniversitySendai 980-8577Japan Institute for Convergence Research and Education in Advanced Technology Yonsei UniversitySeoul 03722Republic of Korea School of Mechanical and Materials Engineering Washington State UniversityPullmanWashington 99164USA School of Mechanical Engineering Purdue UniversityWest LafayetteIndiana 47907USA Research Institute of Advanced Manufacturing Technology Korea Institute of Industrial TechnologyIncheon 21999Republic of Korea Department of Macromolecular Science and Engineering Case Western Reserve UniversityClevelandOhio 44106USA Department of Chemical and Biomolecular Engineering and Joint Institute for Advanced Materials University of TennesseeKnoxvilleTennessee 37996USA Center for Nanophase Materials and Sciences Oak Ridge National LaboratoryOak RidgeTennessee 37830USA Department of Mechanical Engineering University of New BrunswickFrederictonNew Brunswick E3B 5A3Cana

The ex-situ incorporation of the secondary SiC reinforcement,along with the in-situ incorporation of the tertiary and quaternary Mg_(3)N_(2) and Si_(3)N_(4) phases,in the primary matrix of Mg_(2)Si is employed in order to provide ultimate wear resistance based on the laser-irradiation-induced inclusion of N_(2) gas during laser powder bed *** is substantialized based on both the thermal diffusion-and chemical reactionbased metallurgy of the Mg_(2)Si–SiC/nitride hybrid *** study also proposes a functional platform for systematically modulating a functionally graded structure and modeling build-direction-dependent architectonics during additive *** strategy enables the development of a compositional gradient from the center to the edge of each melt pool of the Mg_(2)Si–SiC/nitride hybrid ***,the coefficient of friction of the hybrid composite exhibits a 309.3%decrease to–1.67 compared to–0.54 for the conventional nonreinforced Mg_(2)Si structure,while the tensile strength exhibits a 171.3%increase to 831.5 MPa compared to 485.3 MPa for the conventional *** outstanding mechanical behavior is due to the(1)the complementary and synergistic reinforcement effects of the SiC and nitride compounds,each of which possesses an intrinsically high hardness,and(2)the strong adhesion of these compounds to the Mg_(2)Si matrix despite their small sizes and low concentrations.

关键词： Laser powder bed fusion Mg_(2)Si-SiC/nitride hybrid composite Both the thermal diffusion-and chemical reaction-based metallurgy Functionally graded structure Compositional gradient Wear resistance.

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单边螺栓连接中心支撑钢框架抗震性能分析

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河南理工大学学报（自然科学版） 2024年第2期43卷 166-176页

作者：蔺新艳杨新宇王新武孙海粟刘欢欢 jang Il young 河南理工大学土木工程学院河南焦作454000 洛阳理工学院河南省新型土木工程结构国际联合实验室河南洛阳471023 武汉理工大学理学院湖北武汉430070 金乌国立工科大学土木工程学院韩国龟尾39177

目的为研究支撑-半刚接钢框架结构体系的抗震性能,方法设计了一榀由嵌套式单边螺栓与T型钢构成的半刚性梁柱节点的中心支撑钢框架,并进行了拟静力试验与有限元数值模拟,通过观测整个试验现象,分析了其滞回、承载力、刚度退化、耗能等抗... 详细信息

目的为研究支撑-半刚接钢框架结构体系的抗震性能,方法设计了一榀由嵌套式单边螺栓与T型钢构成的半刚性梁柱节点的中心支撑钢框架,并进行了拟静力试验与有限元数值模拟,通过观测整个试验现象,分析了其滞回、承载力、刚度退化、耗能等抗震指标。结果结果表明:试件破坏过程明显经历了弹性段、塑性段、破坏段三个阶段,试件破坏模式主要为支撑受压失稳破坏,塑性变形主要累积在支撑体系上,整体呈现延性破坏特征;支撑断裂后,梁柱及T型钢节点无明显塑性变形,钢框架仍具有较高的安全储备,符合“强节点、弱构件”设计原则,表明了结构具有两道抗震防线;结论支撑与半刚接钢框架协同工作使得试件具有较高的抗侧刚度抵抗水平变形,且承载力较高、滞回性能稳定、耗能能力优良;单边螺栓在试验过程中的受力性能较普通高强螺栓并无较大差别,未出现严重的预紧力松弛现象,并能高效的保持螺栓预紧力。通过有限元数值模拟分析可知,减小支撑长细比,虽能有效提高结构的抗震性能,但长细比较小会导致支撑刚度增大,加速其余构件的损坏。故应以考虑结构的延性为前提,降低支撑的长细比,才能有效提高结构的抗震性能。

关键词：中心支撑单边螺栓 T型钢半刚性连接抗震性能

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Deep Drawing Process Using a Tractrix Die for Manufacturing Liners for a CNG High-Pressure Vessel(Type Ⅱ)

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Chinese Journal of Mechanical Engineering 2022年第1期35卷 132-143页

作者： Gun‑young Park Hyo‑Seo Kwak Hyo‑Seong jang Chul Kim School of Mechanical Engineering Pusan National UniversityPusan 46241South Korea Department of Mechanical Engineering Dong-eui UniversityPusan 47340Korea.

The liner of a CNG pressure vessel is manufactured by a DDI(deep drawing and ironing)process for the cylinder part,which is a continuous process that includes a drawing process to reduce the diameter of the billet and a subsequent ironing process to reduce the thickness of the billet.A tractrix die used in the 1^(st) deep drawing allows the blank to flow smoothly by decreasing the punch load and radial tensile stress occurring in the *** also increases the draw ratio compared to conventional dies,but it causes forming *** this study,a shape coefficient(S_(c))is proposed for the tractrix die using the blank diameter(D_(0)),inflow diameter of the workpiece(d_(i)),and inflow angle of the workpiece(θ)for design of the tractrix *** effects of the thickness and inflow angle of the workpiece on wrinkling and folding were investigated through ***,a discriminant is proposed for the relative radial stress(σ)generated during the deep drawing process using the tractirx die and used to predict *** on the results,the blank thickness,the draw ratio,and the inflow of the workpiece angle in the 1^(st) deep drawing process are suggested,and the number of operations in the DDI process was reduced from 6 to *** improves the productivity and reduces the manufacturing cost.

关键词： Tractrix die Deep drawing Wrinkling Folding LDR(Limit draw ratio) FEM

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