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Shear deformable finite beam elements for composite box beams

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Acta Mechanica Sinica 2014年第2期30卷 223-240页

作者： nam-Il kim Dong-Ho Choi Department of Architectural Engineering Sejong University Department of Civil and Environmental Engineering Hanyang University

The shear deformable thin-walled composite beams with closed cross-sections have been developed for coupled flexural, torsional, and buckling analyses. A theoretical model applicable to the thin-walled laminated composite box beams is presented by taking into account all the structural couplings coming from the material anisotropy and the shear deformation effects. The current composite beam includes the transverse shear and the restrained warping induced shear deformation by using the first-order shear deformation beam theory. Seven governing equations are derived for the coupled axial-flexural-torsional-shearing buckling based on the principle of minimum total potential energy. Based on the present analytical model, three different types of finite composite beam elements, namely, linear, quadratic and cubic elements are developed to analyze the flexural, torsional, and buckling problems. In order to demonstrate the accuracy and superiority of the beam theory and the finite beam elements developed by this study,numerical solutions are presented and compared with the results obtained by other researchers and the detailed threedimensional analysis results using the shell elements of ABAQUS. Especially, the influences of the modulus ratio and the simplified assumptions in stress-strain relations on the deflection, twisting angle, and critical buckling loads of composite box beams are investigated.

关键词： Thin-walled Composite box beam Deflection Twisting angle Buckling load Shear deformation

Entacapone promotes hippocampal neurogenesis in mice

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Neural Regeneration Research 2021年第6期16卷 1005-1010页

作者： Dae young Yoo Hyo young Jung Woosuk kim Kyu Ri Hahn Hyun Jung Kwon Sung Min nam Jin young Chung Yeo Sung Yoon Dae Won kim In Koo Hwang Department of Anatomy and Cell Biology College of Veterinary Medicineand Research Institute for Veterinary ScienceSeoul National UniversitySeoulSouth Korea Department of Anatomy College of MedicineSoonchunhyang UniversityCheonanSouth Korea Department of Biomedical Sciences and Research Institute for Bioscience and BiotechnologyHallym UniversityChuncheonSouth Korea Department of Biochemistry and Molecular Biology Research Institute of Oral SciencesCollege of DentistryGangneung-Wonju National UniversityGangneungSouth Korea Department of Anatomy College of Veterinary MedicineKonkuk UniversitySeoulSouth Korea Department of Veterinary Internal Medicine and Geriatrics College of Veterinary MedicineKangwon National UniversityChuncheonSouth Korea

Entacapone,a catechol-O-methyltransferase inhibitor,can strengthen the therapeutic effects of levodopa on the treatment of Parkinson’s ***,few studies are reported on whether entacapone can affect hippocampal neurogenesis in *** investigate the effects of entacapone,a modulator of dopamine,on proliferating cells and immature neurons in the mouse hippocampal dentate gyrus,60 mice(7 weeks old)were randomly divided into a vehicle-treated group and the groups treated with 10,50,or 200 mg/kg *** results showed that 50 and 200 mg/kg entacapone increased the exploration time for novel object *** staining results revealed that after entacapone treatment,the numbers of Ki67-positive proliferating cells,doublecortin-positive immature neurons,and phosphorylated cAMP response element-binding protein(pCREB)-positive cells were significantly *** blot analysis results revealed that treatment with tyrosine kinase receptor B(TrkB)receptor antagonist significantly decreased the exploration time for novel object recognition and inhibited the expression of phosphorylated TrkB and brain-derived neurotrophic factor(BDNF).Entacapone treatment antagonized the effects of TrkB receptor *** results suggest that entacapone treatment promoted hippocampal neurogenesis and improved memory function through activating the BDNF-TrkB-pCREB *** study was approved by the Institutional Animal Care and Use Committee of Seoul National University(approval ***-130730-1)on February 24,2014.

关键词： brain-derived neurotrophic factor entacapone hippocampus neurogenesis neurotrophic factor phosphorylated cAMP response element-binding protein tyrosine kinase receptor B receptor

Bamboo salt suppresses skin inflammation in mice with 2, 4-dinitrofluorobenzene-induced atopic dermatitis

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Chinese Journal of Natural Medicines 2018年第2期16卷 97-104页

作者： Myoung-schook Yoou Sun-young nam Kyoung Wan Yoon Hyun-Ja Jeong Hyung-Min kim Department of Pharmacology College of Korean Medicine Kyung Hee University Department of Biotechnology Hoseo University Department of Food Science & Technology and Research Institute for Basic Science Hoseo University

Bamboo salt(BS) is a traditional Korean food, and has been reported to have anti-cancer, anti-inflammatory, and anti-metastatic effects. However, the anti-atopic dermatitis(AD) activity of BS has not been described yet. In the present study, we examined the preventive effect of BS on AD. The effect of oral administration of BS was tested in a 2, 4-dinitrofluorobenzene(DNFB)-induced AD animal model, by histological analysis, enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction, caspase-1 assay, and Western blotting analysis. BS administration reduced the total clinical severity and scratching frequencies, compared with the AD group. In the serum of DNFB-induced AD mice, the levels of IgE, histamine, thymic stromal lymphopoietin(TSLP), interleukin(IL)-5, and IL-13 were significantly reduced by BS treatment. BS significantly reduced the protein and mRNA expression of TSLP, IL-6, and tumor necrosis factor-α in the AD skin lesions. BS markedly reduced the infiltration of inflammatory cells. Furthermore, the activation of caspase-1 was reduced by BS in the AD skin lesions. Our results suggested that BS should be considered as a candidate treatment for allergic inflammatory diseases including AD.

关键词： Bamboo salt Atopic dermatitis Thymic stromal lymphopoietin Caspase-1

Anti-senescence and anti-wrinkle activities of 3-bromo-4,5-dihydroxybenzaldehyde from Polysiphonia morrowii Harvey in human dermal fibroblasts

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Asian Pacific Journal of Tropical Biomedicine 2021年第2期11卷 74-80页

作者： Su-Hyeon Cho Eun-Yi Ko Soo-Jin Heo Seo-young kim Juhee Ahn Kil-nam kim Chuncheon Center Korea Basic Science InstituteChuncheon 24341Republic of Korea Bio research Center DermaproJeju 63309Republic of Korea Jeju Marine Research Center Korea Institute of Ocean Science&TechnologyJeju 63349Republic of Korea Department of Medical Biomaterials Engineering College of Biomedical SciencesKangwon National UniversityChuncheon 24341Republic of Korea

Objective:To investigate the anti-senescence effect of 3-bromo-4,5-dihydroxybenzaldehyde(BDB)from Polysiphonia morrowii Harvey in human dermal fibroblasts(HDF).Methods:HDF were subjected to treatment of BDB and then treated with hydrogen peroxide(H2O2)to induce premature ***-associatedβ-galactosidase(SA-β-gal)activity in HDF was determined using the SA-β-gal staining *** reactive oxygen species(ROS)production was measured using the 2’,7’-dichlorodihydrofluorescein diacetate *** blotting assay was performed to assess the level of antioxidant enzyme glutathione peroxidase 1(GPX1).In addition,intracellular collagen and collagenase contents were analyzed using the respective ELISA *** activity in HDF supernatants was measured from p-nitroaniline release and normalized using total protein ***:Treatment of HDF with H2O2 increased the activity of SA-β-gal,but BDB pre-treatment resulted in the reduction of SA-β-gal ***,BDB significantly reduced H2O2-induced intracellular ROS *** also markedly increased the level of GPX1,which was inhibited by 400μM of ***,in in vitro study,BDB significantly increased intracellular collagen content and decreased matrix metalloproteinase-1 and elastase activities in ***:Our results demonstrate that BDB shows antisenescence and anti-wrinkle activities in vitro.

关键词： Polysiphonia morrowii Harvey 3-bromo-4,5-dihydroxybenzaldehyde Oxidative stress Human dermal fibroblast Anti-senescence activity Anti-wrinkle activity

Interstitial tissue-specific gene expression in mouse testis by intra-tunica albuguineal injection of recombinant baculovirus

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Asian Journal of Andrology 2009年第3期11卷 342-350页

作者： Hyun Jung Park Won young Lee Jin Hoi kim Jae Hwan kim Hun Jong Jung nam Hyung kim Bo Kyung kim Hyuk Song Department of Animal Science College of Natural Science Konkuk University Chung-ju 380-701 Korea CHA Stem Cell Institute Graduate School of Life Science and Biotechnology Pochon CHA University Seoul 135-907 Korea Department of Animal Biotechnology College of Animal Biotechnology Konkuk University Seoul 143-701 Korea Occupation and Environment Department Medical School Konkuk University Chung-ju 380-704 Korea Department of Animal Science College of Agriculture Chungbuk National University Choung-ju Chung-buk 361-763 Korea Department of Physiology Medical School Konkuk University Chung-ju 380-701 Korea

The purpose of this study is to establish a gene delivery system for interstitial tissue-specific protein expression in mice testes using modified recombinant baculovirus. Green fluorescent protein （GFP）-expressing recombinant baculovirus （GFP-baculovirus）, in which the insect cell-specific polyhedron promoter was replaced by the cytomegalovirus （CMV）-IE promoter, was used to transfect testicular cells in vitro, and for intra-tunica albuguineal injection of the interstitial tissue of the testis. GFP expression was monitored in frozen testes sections by fluorescence microscopy. Expression of GFP in testicular tissues was also assessed by reverse transcription polymerase chain reaction （RT-PCR）, and protein expression was assessed by Western blot. Testicular cells in vitro were infected efficiently by modified recombinant GFP-baculovirus. lntra-tunica albuguineal injection of GFP- baculovirus into the mouse testis resulted in a high level of GFP expression in the interstitial tissues. RT-PCR analysis clearly showed GFP gene expression in the testis, particularly interstitial tissues. Intra-tunica albuguineal injection of a modified baculovirus that encoded recombinant rat insulin-like growth factor binding protein （IGFBP）-5 resulted in an increase in IGFBP-5 in testis and semen. In conclusion, we have developed an efficient delivery system for gene expression in vivo in testicular cells, particularly cells of the interstitial tissue using intratunica albuguineal injection of a modified recombinant baculovirus. This method will be particularly relevant for application that requires gene delivery and protein expression in the testicular cells of the outer seminiferous tubule of the testis.

关键词： intra-tunica albuguineal injection recombinant baculovirus testis gene delivery

Refractory lipoatrophy treated with autologous whole blood injection:A case report

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World Journal of Clinical Cases 2025年第2期13卷 33-38页

作者： Wonnam kim Jun Su Park En Hyung kim Department of Pharmacology School of Korean MedicinePusan National UniversityYangsan 50612GyeongnamSouth Korea Department of Dermatology College of MedicineChungbuk National UniversityCheongju 28644ChungbukSouth Korea Department of Dermatology Chungbuk National University HospitalCheongju 28644ChungbukSouth Korea

BACKGROUND Intramuscular corticosteroid injection may cause adverse effects such as dermal and/or subcutaneous atrophy,alopecia,hypopigmentation,and *** cutaneous atrophy can spontaneously resolve,several treatment options have been suggested for this *** SUMMARY In this paper,we report a case of corticosteroid injection induced lipoatrophy treated with autologous whole blood(AWB)injection,as the condition had been unresponsive to fractional laser therapy.A 29-year-old female patient visited the dermatology clinic complaining of skin depression on her right buttock area,which had appeared six months *** had been only subtle improvement at the margins after fractional CO_(2) laser treatment;therefore,after obtaining informed consent from the patient,AWB treatment was *** month after the first AWB injection,the size and depth of the lesion had noticeably improved,and a slight improvement was also observed in *** Close observation is the initial treatment of choice for steroid induced skin atrophy;however,for patients in need of immediate cosmetic improvement,AWB injection may be a safe and cost-effective alternative.

关键词： Atrophy Corticosteroid Subcutaneous fat Autologous whole blood injection Laser therapy Case report

Cardiac metastasis from colorectal cancer:A case report

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World Journal of Gastroenterology 2009年第21期15卷 2675-2678页

作者： Pyong Wha Choi Chul nam kim Sun Hee Chang Woo Ik Chang Chang young kim Hyun Min Choi Department of Surgery Ilsan Paik HospitalInje University College of Medicine Department of Diagnostic Pathology Ilsan Paik HospitalInje University College of Medicine Department of Chest Surgery Ilsan Paik HospitalInje University College of Medicine Department of Internal Medicine Ilsan Paik HospitalInje University College of Medicine

The heart is an unusual site of metastasis from any malignancy. We report a case of cardiac metastasis from colorectal cancer. A 70-year-old woman was referred with a presumptive diagnosis of sigmoid colon cancer with cardiac myxoma. Two-dimensional echocardiography showed a 4 cm x 4.5 cm mobile mass on the lateral right atrial wall, and computed tomography revealed a low attenuated Iobulating mass in the right atrium. The patient underwent anterior resection for sigmoid colon cancer （T4N2）. Thereafter, she experienced progressive shortness of breath. Therefore, a cardiac operation was performed 2 wk after the colorectal operation. Histological examination revealed adenocarcinoma, which was identical to the primary lesion. Although two- dimensional echocardiography has become the diagnostic test of choice for detecting cardiac tumors, in patients with colorectal cancer showing a cardiac mass, further diagnostic evaluation such as a magnetic resonance imaging might be necessary.

关键词： Heart Cardiac metastasis Colorectalcancer Atrial myxoma Magnetic resonance imaging

Characteristics of Algal Succession Following Rock Scraping at Imwon Area in the East Coast of Korea

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Journal of Ocean University of China 2016年第6期15卷 1087-1093页

作者： young Dae kim Jung Kwan Alan Myung Mo nam Chu Lee Hyun Il Yoo Su Yeoung Yeon young Hwan kim Jang Kyun kim Jae Suk Choi East Sea Fisheries Research Institute National Fisheries Research & Development Institute Gangneung 210-860Republic of Korea Korea National Park Service National Park Research Institute Yeosu 550-833 Republic of Korea Seaweed Research Center 1rational Fisheries Research & Development lnstituie Mokpo 530-831 Republic of Korea Department of Biology Chungbuk National University Chongju 361-763 Republic Of Korea Department of Marine Science School of Natural Sciences Incheon National University 22012 Yeonsu-gu Incheon Korea Department of Marine Sciences University of Connecticut 06901 Stamford Connecticut USA Division of Bioindustry Silla University Sasang-gu Busan 617-736 Republic of Korea

Abstract This study was conducted to clarify the characteristics high-pressure water sprayer in the period from June 2010 to April 2011 of algal succession following rock scraping using hoe or We divided the research area off the eastern coast of Korean near Imwon into 3 categories depending upon the severity of the barren ground, i.e., the urchin barren-affected, urchin bar- ren-ongoing and urchin barren-free areas. In April 2011, in the urchin barren-affected area with 25 seaweed species, the cover per- centage and importance value （IV） of erustose coralline algae were higher than those of other species. In the urchin barren-ongoing area with 33 seaweed species, crustose coralline algae （mean IV=62%） as well as Sargassum sp. （mean IV=28%）, and Gelidium amansii （mean IV= 19%） were observed following rock scraping. In the urchin barren-free area where seaweed communities were relatively abundant with 42 species, a variety of algal species including G. amansii （mean IV = 32%） underwent algal succession. Overall, it was observed that, as an aspect of algal succession, the weaker the barren ground severity was, the more frequent and di- verse the seaweeds were, and the more complex the succession pattern was in the study. As an aspect of recovering algal community, rock scraping using hoe was shown to be superior to the method using high-pressure water spraying. Therefore, we conclude that rock scraping using hoe is a very effective strategy for recovering the algal community in urchin barren-ongoing area.

关键词： algal succession sea-urchin barren ground rock scraping subtidal zone

Effects of Lizhong Tang on cultured mouse small intestine interstitial cells of Cajal

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World Journal of Gastroenterology 2013年第14期19卷 2249-2255页

作者： Min Woo Hwang Jung nam kim Ho Jun Song Bora Lim young Kyu Kwon Byung Joo kim Department of Sasang Constitutional Medicine College of Korean MedicineKyung-hee University School of Korean Medicine Pusan National University

AIM:To investigate the effects of Lizhong Tang,an herbal product used in traditional Chinese medicine,on mouse small intestine interstitial cells of Cajal(ICCs).METHODS:Enzymatic digestions were used to dissociate ICCs from mouse small intestine *** ICCs were morphologically distinct from other cell types in culture and were identified using phase contrast microscopy after verification with anti c-kit antibody.A whole-cell patch-clamp configuration was used to record potentials(current clamp) from cultured *** of the experiments were performed at 30-32 ℃.RESULTS:ICCs generated pacemaker potentials,and Lizhong Tang produced membrane depolarization in current-clamp *** application of flufenamic acid(a nonselective cation channel blocker) abolished the generation of pacemaker potentials by Lizhong *** with thapsigargin(a Ca 2+-ATPase inhibi-tor in the endoplasmic reticulum) also abolished the generation of pacemaker potentials by Lizhong ***,pacemaker potentials were completely abolished in the presence of an external Ca 2+-free solution,and under this condition,Lizhong Tang induced membrane ***,When GDPβ-S(1 mmol/L) was in the pipette solution,Lizhong Tang still induced membrane *** addition,membrane depolarizations were not inhibited by chelerythrine or calphostin C,which are protein kinase C inhibitors,but were inhibited by U-73122,an active phospholipase C ***:These results suggest that Lizhong Tang might affect gastrointestinal motility by modulating pacemaker activity in interstitial cells of Cajal.

关键词： Interstitial cells of Cajal Lizhong Tang Motility Gastrointestinal tract Whole-cell patch clamp configuration