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Efficiency for Gene Silencing Induction in Nicotiana Species by a Viral Satellite DNA Vector

Journal of Integrative Plant Biology 2007年第12期49卷 1726-1733页

作者： you-ping xu Lu-ping Zheng Qiu-Fang xu Chang-Chun Wang xue-ping Zhou Zu-Jian Wu Xin-Zhong Cai Institute of Biotechnology Zhejiang University Hangzhou 310029 China Centre of Analysis and Measurement Zhejiang University Hangzhou 310029 China Institute of Plant Virology Fujian Agriculture and Forestry University Fuzhou 350002 China

Virus-induced gene silencing （VIGS） is a useful technique for rapid plant gene function analysis. We recently reported a new VIGS vector modified from Tomato yellow leaf curl China virus （TYLCCNV） DNAβ （DNAm β）. In this study we compared in detail DNAmβ-induced gene silencing in four Nicotiana species including N. benthamiana, N. glutinosa, N. tabacum and N. paniculata. We found that DNAmβ-induced gene silencing in the four species was distinct in developing dynamics, tissue specificity, efficiency, and constancy in the plant life span. It was most efficient in N. benthamiana, where development of VIGS was most rapid, without tissue specificity and nearly 100% efficient. DNAmβ-induced gene silencing in N. glutinosa was also efficient despite being slightly less than in N. benthamiana. It initially occurred in veins, later was scattered to mesophyll, finally led to complete silencing in whole leaves. In both species, VIGS constantly expressed until the plants died. However, DNAmβ-mediated VIGS in the other two Nicotiana species, N. tabacum and N. paniculata, was significantly less efficient. It was strictly limited within the veins of the silenced leaves, and constantly occurred only over 3-4 weeks. The upper leaves that emerged later stopped showing the silencing phenotype. DNAmβ-induced gene silencing in N. benthamiana and N. glutinosa was not significantly influenced by the growth stage when the plants were agro-inoculated, and was not sensitive to high growth temperature up to 32℃. Our results indicate that this system has great potential as a versatile VIGS system for routine functional analysis of genes in some Nicotiana species.

关键词： DNAβ Nicotiana tobacco rattle virus tomato yellow leaf curl China virus virus-induced gene silencing.

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Leaf stage-associated resistance is correlated with phytohormones in a pathosystem-dependen manner

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Journal of Integrative Plant Biology 2018年第8期60卷 703-722页

作者： you-ping xu Lin-Hui Lv Ya-Jing xu Juan Yang Jia-Yi Cao Xin-Zhong Cai State Key Laboratory of Rice Biology Institute of Biotechnology College of Agriculture and Biotechnology Zhejiang University 866 Yu Hang Tang Road Hangzhou 310058 China Centre of Analysis and Measurement Zhejiang University 866 Yu Hang Tang Road Hangzhou 310058 China

It has been reported in several pathosystems that disease resistance can vary in leaves at different stages. However, how general this leaf stage-associated resistance is, and the molecular mechanism（s） underly- ing it, remain largely unknown. Here, we investigated the effect of leaf stage on basal resistance, effector- triggered immunity （ETI） and nonhost resistance, using eight pathosystems involving the hosts Arabidopsis thaliana, Nicotiana tabacum, and N. benthamiana and the pathogens Sclerotinia sclerotiorum, Pseudomonas syringae pv. tabaci, P. syringae pv. tomato DC3000, and Xanthomonas oryzae pv. oryzae （Xoo）. We show evidence that leaf stage-associated resistance exists ubiquitously in plants, but with varying intensity at different stages in diverse pathosystems. Microarray expression profiling assays demonstrated that hundreds of genes involved indefense responses, phytohormone biosynthesis and signaling, and calcium signaling, were differentially expressed between leaves at different stages. The Arabidopsis mutants sid1, sid2-3, ein2, jar1-1, abal and aao3 lost leaf stage-associated resistance to S. sclerotiorum, and the mutants abal and sid2-3 were affected in leaf stage-associated RPS2/AvrRpt2＋-con- ferred ETI, whereas only the mutant sid2-3 influenced leaf stage-associated nonhost resistance to Xoo. Our results reveal that the phytohormones salicylic acid, ethylene, jasmonic acid and abscisic acid likely play an essential, but pathosystem-dependent, role in leaf stage- associated resistance.

关键词： Xoo Figure Leaf stage-associated resistance is correlated with phytohormones in a pathosystem-dependen manner

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RALF22 promotes plant immunity and amplifies the Pep3 immune signal

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Journal of Integrative Plant Biology 2023年第11期65卷 2519-2534页

作者： Yu-Han He Song-Yu Chen Xing-Yan Chen you-ping xu Yan Liang Xin-Zhong Cai Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province Institute of BiotechnologyCollege of Agriculture and BiotechnologyZhejiang University866 Yu Hang Tang RoadHangzhou 310058China Centre of Analysis and Measurement Zhejiang University866 Yu Hang Tang RoadHangzhou 310058China Hainan Institute Zhejiang UniversitySanya 572025China

Rapid alkalinization factors(RALFs)in plants have been reported to dampen pathogenassociated molecular pattern(PAMP)-triggered immunity via suppressing PAMP-induced complex formation between the pattern recognition receptor(PRR)and its co-receptor ***,the direct and positive role of RALFs in plant immunity remains largely ***,we report the direct and positive roles of a typical RALF,RALF22,in plant ***22alone directly elicited a variety of typical immune responses and triggered resistance against the devastating necrotrophic fungal pathogen Sclerotinia sclerotiorum in a FERONIA(FER)-dependent ***(LRE)-like glycosylphosphatidylinositol(GPI)-anchored protein 1(LLG1)and NADPH oxidase RBOHD were required for RALF22-elicited reactive oxygen species(ROS)*** mutation of cysteines conserved in the C terminus of RALFs abolished,while the constitutive formation of two disulfide bridges between these cysteines promoted the RALF22-elicited ROS production and resistance against ***,demonstrating the requirement of these cysteines in the functions of RALF22 in plant ***,RALF22 amplified the Pep3-induced immune signal by dramatically increasing the abundance of PROPEP3 transcript and *** with RALF22 induced resistance against *** in Brassica crop ***,our results reveal that RALF22 triggers immune responses and augments the Pep3-induced immune signal in a FER-dependent manner,and exhibits the potential to be exploited as an immune elicitor in crop protection.

关键词： RALF22 FER reactive oxygen species Pep3 plant immunity Sclerotinia sclerotiorum

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基于在线评论情感语义分析和TOPSIS法的酒店服务质量测量

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情报科学 2017年第10期35卷 13-17页

作者：由丽萍白旭云山西大学经济与管理学院山西太原030006 山西财经大学工商管理学院山西太原030012

【目的/意义】为解决服务质量评价难以量化、数据来源可信度低等问题,本文提出一种在线评论情感分析和多属性法相结合的酒店服务质量测量模型。【方法/过程】以在线评论的情感语义分析作为服务质量测量的数据来源,以TOPSIS多属性法作为... 详细信息

【目的/意义】为解决服务质量评价难以量化、数据来源可信度低等问题,本文提出一种在线评论情感分析和多属性法相结合的酒店服务质量测量模型。【方法/过程】以在线评论的情感语义分析作为服务质量测量的数据来源,以TOPSIS多属性法作为测量方法。【结果/结论】通过实验分析,证明了研究方法的有效性和科学性。其研究结果既作为情感分析研究走向应用的一次探索,也为服务质量评价的研究提供了一条可行的技术路线。

关键词：框架语义情感分析 TOPSIS法服务质量管理

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斜切进刀时采煤机滚筒力学特性分析

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工程设计学报 2015年第6期22卷 613-618页

作者：毛君潘德文李强谢苗由旭平辽宁工程技术大学机械工程学院辽宁阜新123000 宿州学院机械与电子工程学院安徽宿州234000

为了分析采煤机斜切进刀时滚筒力学特性,推导了采煤机在斜切进刀时单个截齿的截割阻力,应用叠加法得出了滚筒截齿总截割阻力,并运用挤压面积和煤岩破裂理论得出了滚筒轴向力.同时,以国产的MG400/940-WD交流电牵引采煤机为例,利用MATLAB... 详细信息

为了分析采煤机斜切进刀时滚筒力学特性,推导了采煤机在斜切进刀时单个截齿的截割阻力,应用叠加法得出了滚筒截齿总截割阻力,并运用挤压面积和煤岩破裂理论得出了滚筒轴向力.同时,以国产的MG400/940-WD交流电牵引采煤机为例,利用MATLAB对单个截齿的截割阻力、滚筒截齿总截割阻力及滚筒轴向力进行模拟.模拟结果表明:前滚筒最大轴向力约为最大总截割阻力的3.64倍,后滚筒最大轴向力约为最大总截割阻力的3.32倍.模拟结果对提高采煤机滚筒的稳定性及截齿的可靠性有一定的参考价值.

关键词：采煤机滚筒斜切进刀截割阻力轴向力

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室内外培养对耐温微藻Desmodesmus sp.F51细胞生长和叶黄素积累的影响

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过程工程学报 2016年第5期16卷 849-855页

作者：谢友坪阳需求陈剑锋卢英华福州大学生物科学与工程学院福建福州350108 厦门大学化学工程与生物工程系福建厦门361005

在室内培养耐温微藻F51,考察了培养温度、自来水及非灭菌培养基对细胞生长及叶黄素积累的影响,并与50 L柱状光生物反应器户外分批培养情况进行对比.结果表明,藻株在35℃下的生物质产率和叶黄素产率最大,分别为767.5和3.05mg/（L·d）.... 详细信息

在室内培养耐温微藻F51,考察了培养温度、自来水及非灭菌培养基对细胞生长及叶黄素积累的影响,并与50 L柱状光生物反应器户外分批培养情况进行对比.结果表明,藻株在35℃下的生物质产率和叶黄素产率最大,分别为767.5和3.05mg/（L·d）.低温培养有利于叶黄素积累,但生长速率减缓.用自来水及非灭菌培养基培养时,藻株无明显的生长抑制及染菌现象,在13.0-27.6℃户外培养温度下,藻株最高生物质产率、二氧化碳固定率和叶黄素产率分别为236.3,444.2和1.17 mg/（L·d）.

关键词：耐温微藻叶黄素室内培养室外培养

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浙江优秀民营企业走可持续发展之路的理论与实践

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华东经济管理 2005年第3期19卷 15-17页

作者：游旭平裘益政浙江工商大学公共管理学院浙江杭州310035 浙江工商大学财会学院浙江杭州310035

企业是利益相关人之间契约的集合。民营企业利益相关人包括投资人、债权人、经营者、员工、政府等。在民营企业发展的第二个阶段,为了实现可持续发展,必须注重相关人利益。对浙江优秀民营企业的调查表明,注重相关人利益已经成为它们在... 详细信息

企业是利益相关人之间契约的集合。民营企业利益相关人包括投资人、债权人、经营者、员工、政府等。在民营企业发展的第二个阶段,为了实现可持续发展,必须注重相关人利益。对浙江优秀民营企业的调查表明,注重相关人利益已经成为它们在新时期实现第二次飞跃的重要基础。

关键词：利益相关人浙江民营企业

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Insights from untargeted metabolomics: revealing potential marker compounds into the maturation of fermented chili

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Food Science and Human Wellness 2025年

作者： Shiyu Liu Mingyuan Yang Jianhua Zhao Meijuan Lei Jinlin Han Min xu ping Liu Jie Tang Hongbin Lin School of Food and Bio-engineering， Xihua University China Agricultural University-Sichuan Advanced Agricultural & Industrial Institute Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing Food Microbiology Key Laboratory of Sichuan Province

Chili fermentation is one of the most important processes in the production of Pixian Douban (PXDB)， which determines the flavor and product quality of PXDB. However， the maturity of fermented chili mainly depends on empirical judgment， which can not satisfy the need for standardized production of PXDB. Therefore， this study aimed to investigate volatile and non-volatile substances during the maturation process and to find markers related to the maturity of fermented chili. GC×GC-MS combined with multivariate statistical analysis revealed that 2-methoxy-3-isobutyl pyrazine， linalool， 3-(methylthio)propionaldehyde， myrcene， and decanal (ROAV ≥ 1， VIP > 1， P < 0.05) were regarded as potentially active aromatic markers for differentiating fermentation time. Additionally， UPLC-QTOF-MS combined with multivariate statistical analysis resulted that 14 compounds can serve as differentiated non-volatile compounds. The correlation of maturity-related physico-chemical indicators with volatile and non-volatile compounds revealed that three volatile compounds (2-methoxy-3-isobutylpyrazine， linalool， and decanal) along with fructose and adenosine could serve as markers for evaluating the maturity of chili. This study is expected to establish a standard for the determination of maturity the fermented chili and lay the foundation for intelligent production of PXDB.

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山西省非农就业及收入决定实证分析

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西北农林科技大学学报（社会科学版） 2006年第4期6卷 35-41页

作者：杨金风游旭平史江涛浙江大学管理学院杭州310029 浙江工商大学公共管理学院杭州310035

利用山西省农户调查的微观数据,建立了农户就业天数决定函数和收入决定函数,通过多元回归分析,分别考察了农户户主个人属性、家庭属性、人力资本、村庄属性对农户就业天数和收入的影响。结果显示,在各属性中,人力资本在就业天数和收入... 详细信息

利用山西省农户调查的微观数据,建立了农户就业天数决定函数和收入决定函数,通过多元回归分析,分别考察了农户户主个人属性、家庭属性、人力资本、村庄属性对农户就业天数和收入的影响。结果显示,在各属性中,人力资本在就业天数和收入决定中发挥了重要作用。其中,初中水平的劳动力与其总就业、非农就业和外出就业天数正相关。拥有技术职称、受过培训、健康自评对劳动力各就业天数、收入均有不同程度的影响。

关键词：非农就业收入人力资本

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Modular assembly of complex C-alkyl glycoside enabled via cooperative NHC and photoredox catalysis

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Science China Chemistry 2025年

作者： Rui-Qiang Jiao Ming Li Xi Chen Mei-Ze xu Tian Ding Xiao-ping Gong Zhuo-Mei Li xue-Yuan Liu Yong-Min Liang State Key Laboratory of Applied Organic Chemistry Lanzhou University College of Chemistry and Chemical Engineering Northwest Normal University

C-Alkyl glycosides are found in natural products and are indispensable in drug development programs,owing to their diverse range of medicinal ***-component reactions involving glycosyl radical addition are well documented and proved efficient for the C-alkyl glycosides ***,multicomponent reactions for the rapid and controllable construction of structurally diversified C-alkyl glycosides in a single step remains a significant challenge due to the issues associated with balancing reactivity,compatibility,and ***,we report an NHC and photoredox co-catalyzed three-component method to access complex and valuable C-alkyl *** protocol exhibits broad substrate scope and excellent chemoand ***,nine types of sugars are compatible with this *** addition,the generality of this catalytic strategy was further highlighted by the potential for scalable production,the feasibility of further modifications of the products and its successful application in the late-stage functionalization of pharmaceutical skeletons.

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