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Expression,Characterization and Immunogenicity of a Major Outer Membrane Protein from Vibrio alginolyticus

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Acta Biochimica et Biophysica Sinica 2007年第3期39卷 194-200页

作者： Ronghua QIAN~(1,2) Wuying chu~3 Zhijuan MAO~(1,4) Chongwen ZHANG~(1,2) Yongwei WEI~2 lian YU~2* 1 Department of Biomedical Engineering,Zhejiang University,Hangzhou 310027,China 2 Institute of Preventive Veterinary Medicine,Zhejiang University,Hangzhou 310029,China 3 Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China 4 Ningbo Oceanography and Fishery Institute,Ningbo 315012,China Department of Biomedical Engineering Zhejiang UniversityHangzhou 310027 China Institute of Preventive Veterinary Medicine Zhejiang UniversityHangzhou 310029 China Institute of Subtropical Agriculture Chinese Academy of SciencesChangsha 410125 China Ningbo Oceanography and Fishery InstituteNingbo 315012 China

Vibrio alginolyticus is one of the Vibrio pathogens common to humans and marine *** infection and induction of the host immune response,outer membrane proteins of bacteria play animportant *** this study,an outer membrane protein gene(ompW)was cloned from *** andexpressed in Escherichia *** 645 bp open reading frame(ORF)encodes a protein of 214 amino acidresidues with a predicted molecular weight of 23.3 *** amino acid sequence showed a high identitywith that of Photobacterium damselae(96.2%)and Vibrio parahaemolyticus(94.4%).The alignment analy-sis indicated that OmpW was highly *** dodecyl sulfate-polyacrylamide gel electrophoresisshowed that the gene was over-expressed in *** BL21(DE3).Western blot analysis revealed that theexpressed protein had *** recombinant protein was purified by affinity chromatographyon Ni-NTA Superflow *** yellow croaker vaccinated with the purified OmpW showed significantlyincreased antibody to OmpW,which could resist the infection by ***.A specific antibody wasdetected by enzyme-linked immunosorbent *** study suggested that the conserved OmpW could bean effective vaccine candidate against infection by ***.

关键词： Vibrio alginolyticus ompW expression characterization immunogenicity vaccine candidate

Large magnetic moment at sheared ends of single-walled carbon nanotubes

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Chinese Physics B 2018年第12期27卷 510-516页

作者： Jian Zhang Ya Deng Ting-Ting Hao Xiao Hu Ya-Yun Liu Zhi-Sheng Peng Xian-Nian Chi Pei Wu Si-Yu Liu Zhong Zhang Jun-Jie Li Gong-Tang Wang Wei-Guo chu Chang-Zhi Gu lian-Feng Sun CAS Key Laboratory of Nanosystem and Hierarchical Fabrication CAS Center for Excellence in NanoscienceNational Center for Nanoscience and Technology University of Chinese Academy of Sciences Institute of Physics Chinese Academy of Sciences (CAS) School of Physics and Electronics Shandong Normal University

In this work we report that after single-walled carbon nanotubes(SWNTs) are sheared with a pair of titanium scissors,the magnetization becomes larger than that of the corresponding pristine ones. The magnetization increases proportionally with the number of SWNTs with sheared ends, suggesting that there exist magnetic moments at the sheared ends of *** using the coefficient of this linear relation, the average magnetic moment is estimated to be 41.5 ± 9.8 μB(Bohr magneton) per carbon atom in the edge state at temperature of 300.0 K, suggesting that ultrahigh magnetic fields can be produced. The dangling sigma and pi bonds of the carbon atoms at sheared ends play important roles in determining the unexpectedly high magnetic moments, which may have great potential applications.

关键词： carbon nanotubes shear open ends magnetic moments

化学诱导剂5-氮杂胞苷及模拟生物微环境对骨髓间充质干细胞分化成心肌样细胞的影响

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中国组织工程研究与临床康复 2009年第40期13卷 7931-7936页

作者：初志辉惠杰韩莲花苏州大学附属第一医院心内科江苏省苏州市215006

背景:研究显示干细胞有随环境发生分化即"环境依赖性分化"的特性,但骨髓间充质干细胞在心肌组织是否存在此种特性目前尚未形成公识。目的:观察化学诱导剂和模拟的生物微环境诱导对骨髓间充质干细胞向心肌样细胞分化的影响。设计、时间... 详细信息

背景:研究显示干细胞有随环境发生分化即"环境依赖性分化"的特性,但骨髓间充质干细胞在心肌组织是否存在此种特性目前尚未形成公识。目的:观察化学诱导剂和模拟的生物微环境诱导对骨髓间充质干细胞向心肌样细胞分化的影响。设计、时间及地点:随机分组设计,细胞学体外对比观察,于2008-01/2009-02在苏州大学心血管内科干细胞移植实验室完成。材料:6～8周龄SD大鼠,用于骨髓间充质干细胞的培养;新生1～3d同种系SD大鼠,用于心肌细胞的培养。方法:取SD大鼠股骨骨髓,培养至第2代,经流式细胞仪检测骨髓间充质干细胞纯度97%以上,制成4×108L-1的细胞悬液,实验分为4组:单纯DMEM培养组:用含体积分数为10%胎牛血清的DMEM培养基继续培养;5-氮杂胞苷组:24h后培养液内加入10μmol/L的5-氮杂胞苷;心肌细胞裂解液组:将4倍于骨髓间充质干细胞数量的心肌细胞裂解液加入骨髓间充质干细胞培养瓶中;间接接触组:在培养体系中放入millicell插入式细胞培养皿,28d收获细胞。主要观察指标:免疫组织化学法检测诱导后骨髓间充质干细胞表达心肌特异性肌钙蛋白T、连接蛋白43、CD31的情况;RT-PCR法检测各组早期心肌细胞特异性转录因子GATA4、NKX2.5、β-肌球蛋白重链及α-肌球蛋白重链基因的表达情况。结果:免疫组织化学检测显示实验组诱导4周后特异性抗原呈阳性表达,实验组与单纯DMEM培养组相比,差异有显著性意义(P0.05);CD31是血管内皮细胞的表面抗原标志,单纯DMEM培养组和5-氮杂胞苷组无表达,其他两组弱阳性表达,组间差异无显著性意义(P>0.05)。实验组诱导4周后,均表达心肌前体细胞特异性转录因子NKX-2.5和GATA4,同时表达胚胎期占优势的心肌细胞特异性β-肌球蛋白重链,而没有表达成年期占优势的心肌细胞特异性标志α-肌球蛋白重链。结论:5-氮杂胞苷可以诱导骨髓间充质干细胞向心肌样细胞分化,心肌细胞裂解液和细胞间接接触模拟的心肌微环境也可以诱导骨髓间充质干细胞分化成心肌样细胞,分化的细胞介于成熟的心肌细胞和心肌祖细胞之间的心肌细胞前体。

关键词：骨髓间充质干细胞心肌细胞心肌裂解液间接接触

VOR and CVOR Index for Health Evaluation of Typical Steppe in Inner Mongolia

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Animal Husbandry and Feed Science 2012年第6期4卷 266-270页

作者： SHAN Gui-lian JIANG Hua YAN Wei-hong Liu Zhong-ling LUO Fu-cheng DUAN Xin-hui chu Xiao-hui Pasture Science Department Yunnan Agricultural University Grassland Research Institute of Chinese Academy of Agricultural Science College of Life Sciences Inner Mongolia University

[Objective] The aim of this study was to evaluate the effect of livestock exclusion on the health of grassland ecosystem accurately and quantitatively. [Method] One open grazing site and five seasonal exclusion sites, which had the same vegetation composition （taking Leymus chinensis and Stipa krylovii as main species） and soil type, were chosen as research objects for the VOR and CVOR evaluation of the effect of open grazing and livestock exclusion on the health of typical steppes, and for building the calculation model for VOR and CVOR indices to evaluate the health of typical steppes with different exclusion periods. [ Result] The VOR and CVOR composite indices both could accurately evaluate the health of typical steppes, and the evaluation results from the both indices were quite consistent. Although the condition （C）, vigor（V）, organization （O） and resilience（ R ） indices changed differently in different exclusion periods, the VOR and CVOR composite indices increased gradually with the peri- od of exclusion prolonged, and the health condition of typical steppe changed from ＂alert＂ level （in the open grazing and earlier exclusion period） to ＂healthy＂ level （after 13 to 20 years＇exclusion）. [ Conclusion] The application of exclusion was beneficial to the recovery of degraded grassland ecosystem.

关键词： Typical steppe Health evaluation Grazing Seasonal exclusion VOR index CVOR index

云南永善苏田剖面寒武系沧浪铺组层序地层与沉积特征

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地层学杂志 2020年第3期44卷 268-276页

作者：文舰彭军陈友莲褚江天邓思思戴鑫西南石油大学地球科学与技术学院四川成都610500 中国石油西南油气田分公司勘探开发研究院四川成都610066

为进一步加强对云南地区沧浪铺组层序地层和沉积特征的认识,本文以野外露头剖面实测资料为基础,运用露头层序地层学和沉积学,对云南永善苏田剖面寒武系沧浪铺组做了详细的研究工作。研究结果显示:沧浪铺组可划分为2个Ⅱ型三级层序(SQ1和... 详细信息

为进一步加强对云南地区沧浪铺组层序地层和沉积特征的认识,本文以野外露头剖面实测资料为基础,运用露头层序地层学和沉积学,对云南永善苏田剖面寒武系沧浪铺组做了详细的研究工作。研究结果显示:沧浪铺组可划分为2个Ⅱ型三级层序(SQ1和SQ2),均由海侵体系域(TST)和高位体系域(HST)构成,层序界面以岩性岩相转换面为主;沧浪铺组主要发育石英砂岩、泥晶灰岩、内碎屑灰岩、泥页岩4种岩石类型,可划分为陆棚相、滨岸相、等斜缓坡相3种相。

关键词：寒武系沧浪铺组层序地层沉积特征苏田剖面云南