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Leptin transiently antagonizes ghrelin and long-lastingly orexin in regulation of Ca^(2+) signaling in neuropeptide Y neurons of the arcuate nucleus

The 26th International Conference on Electrical contacts and the 4th International Conference on Reliability of Electrical Products & Electrical Contacts

作者： Makoto Hasegawa Nanae Kobayashi yoshiyuki kohno Hiroshi Ando Chitose Institute of Science and Technology Kaneka Corporation OsakaJapan

ISBN: (纸本)9781622763136

The authors have already reported that when relays were operated to break load currents in vapors evaporated from an acryl-based non-silicone-type polymeric cured product that can be used as replacement of conventional silicone-type products, the resultant contact resistance characteristics show no *** this paper,the acryl-based non-silicone-type product of UV-radiation-cured type was used,and influences of its vapors on contact resistance characteristics of relays at an ambient temperature of 120℃were ***,a commercially-available mechanical relay(AgSnIn contacts) was sealed into a metal can with the product,and placed in a heating chamber to operate,at an operating frequency of 1 Hz or 0.5 Hz, 40 000 break operations of an inductive DC 14V-1A or 14V-0.4A load *** resistance values were measured at every 50 *** a result,the relays showed no deterioration in contact resistances.

关键词： polyacrylate silicone siloxane contact resistance contact failure arc discharges

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World Journal of Gastroenterology 2008年第41期14卷 6347-6354页

作者： Daisuke kohno Shigetomo Suyama Toshihiko Yada Division of Integrative Physiology Department of Physiology Jichi Medical University School of Medicine Yakushiji 3311-1 Shimotsuke

AIM： To explore the mechanism for interactions of leptin with ghrelin and orexin in the arcuate nucleus （ARC） activating neuropeptide Y （NPY） neurons during physiological regulation of feeding, METHODS： Single neurons from ARC of adult rats with matured feeding function were isolated. [Ca2＋]i was measured to monitore their activities. The time course of leptin effects on ghrelin-induced versus orexin-induced [Ca2＋]i increases in NPY neurons was studied. RESULTS： Administration of ghrelin or orexin-A at 101~ mol/L increased cytosolic Ca2~ concentration （[Ca2＋]~） in NPY neurons isolated from the ARC of adult rats. Upon administration of leptin at 10^-14-10^-12 mol/L, ghrelin-induced [Ca2＋]i increases were initially （〈 10 min） inhibited but later restored, exhibiting a transient pattern of inhibition. In contrast, orexin-induced [Ca2＋]i increases were inhibited by leptin in a long- lasting manner. Furthermore, a prior administration of leptin inhibited orexin action but not ghrelin action to increase ICa 2＋li, CONCLUSION： Leptin counteracted ghrelin effects transiently and orexin effects long-lastingly in NPY neurons. The transient property with which leptin counteracts ghrelin action in NPY neurons may allow the fasting-associated increase in ghrelin levels to activate NPY neurons in the presence of physiological leptin and to stimulate feeding.

关键词： Leptin Ghrelin Orexin Arcuate nucleus Neuropeptide Y Ca2+ Feeding Phosphatidylinositol 3-kinase Phosphodiesterase 3 Signal transducer and activator of transcription 3

Enzyme inhibition assay for pyruvate dehydrogenase complex: Clinical utility for the diagnosis of primary biliary cirrhosis

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World Journal of Gastroenterology 2005年第43期11卷 6735-6739页

作者： Katsuhisa Omagari Hiroaki Hazama Shigeru kohno Second Department of Internal Medicine

Primary biliary cirrhosis (PBC) is usually diagnosed by the presence of characteristic histopathological features of the liver and/or antimitochondrial antibodies (AMA) in the serum traditionally detected by immunofluorescence. Recently, new and more accurate serological assays for the detection of AMA, such as enzyme-linked immunosorbent assay (ELISA), immunoblotting, and enzyme inhibition assay, have been developed. Of these, the enzyme inhibition assay for the detection of anti-pyruvate dehydrogenase complex (PDC) antibodies offers certain advantages such as objectivity, rapidity, simplicity, and low cost. Since this assay has almost 100% specificity, it may have particular applicability in screening the at-risk segment of the population in developing countries. Moreover, this assay could be also used for monitoring the disease course in PBC. Almost all sera of PBC-suspected patients can be confirmed for PBC or non-PBC by the combination results of immunoblotting and enzyme inhibition assay without histopathological examination. For the development of a "complete" or "gold standard" diagnostic assay for PBC, similar assays of the enzyme inhibition for anti-2-oxoglutarate dehydrogenase complex (OGDC) and anti-branched chain oxo-acid dehydrogenase complex (BCOADC) antibodies will be needed in future.

关键词： Primary biliary cirrhosis Enzyme inhibition assay Antimitochondrial antibody 2-oxo-acid dehydrogenasecomplex

Retinoblastoma tumor suppressor functions shared by stem cell and cancer cell strategies

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World Journal of Stem Cells 2016年第4期8卷 170-184页

作者： Susumu kohno Shunsuke Kitajima Nobunari Sasaki Chiaki Takahashi Division of Oncology and Molecular Biology Cancer Research InstituteKanazawa University Department of Medical Oncology Dana-Farber Cancer Institute Department of Pharmacy Graduate School of MedicineKeio University

Carcinogenic transformation of somatic cells resembles nuclear reprogramming toward the generation of pluripotent stem *** events share eternal escape from cellular senescence,continuous self-renewal in limited but certain population of cells,and refractoriness to terminal differentiation while maintaining the potential to differentiate into cells of one or multiple *** represented by several oncogenes those appeared to be first keys to pluripotency,carcinogenesis and nuclear reprogramming seem to share a number of core *** retinoblastoma tumor suppressor product retinoblastoma(RB)seems to be critically involved in both events in highly complicated ***,disentangling such complicated interactions has enabled us to better understand how stem cell strategies are shared by cancer *** review covers recent findings on RB functions related to stem cells and stem cell-like behaviors of cancer cells.

关键词： Stem cells Cancer Retinoblastoma Cancer stem cells

Salinity status of the 2011 Tohoku-oki tsunami affected agricultural lands in northeast Japan

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International Soil and Water Conservation Research 2014年第2期2卷 40-50页

作者： Kingshuk Roy Katsuhiro Sasada Eiichi kohno College of Bioresource Sciences Nihon UniversityJapan

As Japan has abundant annual rainfall(1,000 to 2,500 mm),soil salinity of agricultural lands has rarely been a major *** the 2011 earthquake in the Pacific,large stretches of land along the coast in northeast Japan were devastated by a powerful *** damage of agricultural lands was so severe that agricultural crops could not be grown on large parts of the tsunami-inundated farmlands even two years after the *** paper summarizes the status of agricultural lands in northeast Japan’s Tohoku region that were affected by the *** paper presents the results of a field study of agricultural lands in Miyagi Prefecture,where the extent of the seawater damage was the most severe,representing 67%of the total tsunami-affected agricultural *** samples from surface and underlying(undisturbed)soil were collected from 30 different locations in coastal and tsunami-inundated farmlands and from inland sites located beyond the limit of the tsunami *** analyses and measurements showed that the extent of soil salinity varied greatly across these sites,with the highest electrical conductivity(EC)value of 3.72 dS m^(-1) found in the surface soil of Minamisanriku *** addition,two study sites adjacent to each other,Watari cho and Yamamoto cho,had maximum and minimum EC values of 2.0 dS m^(-1) and 0.21 dS m^(-1),respectively,in their underlying soils.A comparison of the major soil properties revealed that the salinity status of the tsunami-inundated farmlands was dependent on particle size distribution and therefore on the infiltration rate of the soil,as well as the relative physical position(elevation)of the *** study led us to carry out further investigations and experiments(still on-going)related to restoration and mitigation work in the tsunami-inundated agricultural lands,giving the highest priority to the major soil properties of different field sites in Miyagi Prefecture.

关键词： Agricultural land Salinity Soil properties Tsunami Northeast Japan

Case study of high waves in the South Pacific generated by Tropical Cyclone Harold in 2020

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Tropical Cyclone Research and Review 2024年第3期13卷 147-160页

作者： Amit Singh Nadao kohno Hironori Fudeyasu Research Institute of Environment and Information Sciences Yokohama National UniversityYokohamaJapan Meteorological Research Institute Japan Meteorological AgencyTsukubaJapan Typhoon Science and Technology Research Center Yokohama National UniversityYokohamaJapan Fiji Meteorological Service Fiji

This study highlighted a high wave case by severe tropical cyclone Harold and conducted a simulation with a newly developed wave forecasting system for the South Pacific based on the Japan Meteorological Agency third generation wave model(JMA MRI-III)using the National Center for Environment Prediction Global Forecast System(GFS)*** was a very intense tropical cyclone(TC)and very high waves up to 10 m affected parts of Vanuatu and *** model results were reasonable and verified against observations of orbital satellites and a wave buoy at Komave in *** statistical verifications were carefully *** Root Mean Squared Error(RSME),Scatter Index(SI),Bias and R2 are all showing very impressive *** new wave forecasting system is the first high resolution operational model at Fiji Meteorological Service(FMS),which covers the whole Fiji *** system will provide guidance to FMS in preparing marine alerts and warning better and more confidence in providing the marine forecast accurately.

关键词： A wave forecasting system The South pacific Tropical cyclone harold

Gastric mixed adenoma-neuroendocrine tumor:A case report

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World Journal of Clinical Cases 2020年第22期8卷 5744-5750页

作者： Shuzo kohno Hiroaki Aoki Masayuki Kato Masaich Ogawa Kazuhiko Yoshida Department of Surgery The Jikei University Katsushika Medical CenterTokyo 125-8506Katsushika-kuJapan Department of Endoscopy The Jikei University Katsushika Medical CenterTokyo 125-8506Katsushika-kuJapan

BACKGROUND Gastric mixed adenoma-neuroendocrine tumors(NETs)are quite *** the 2019 world health organization classification of tumors of the digestive system,these were designated as a combination of grade 1 or grade 2 NETs and adenomas or tubular *** are no treatment guidelines for these tumors,and pathological and clinical studies are ***,we review previous case reports and present a case of gastric mixed *** SUMMARY A 66-year-old man underwent gastrointestinal endoscopy for the evaluation of upper abdominal *** examination of the biopsy specimen indicated the possibility of gastric cancer.A histopathological examination by endoscopic submucosal dissection showed a mixed adenoma-NET that was completely excised by endoscopic submucosal *** recurrence was observed on gastrointestinal endoscopy at the 6-mo *** Clinicians'awareness of this rare tumor is important for its timely diagnosis and treatment.

关键词： Adenoma Neuroendocrine tumor Diagnosis Pathology Stomach treatment Case report

Involvement of Sphingosine 1-Phosphate (S1P) Receptor Type 1 and Type 4 in Migratory Response of Mouse T Cells toward S1P

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Cellular & Molecular Immunology 2006年第6期3卷 429-437页

作者： Hirofumi Matsuyuki Yasuhiro Maeda Kazuhiro Yano Kunio Sugahara Kenji Chiba Takayuki kohno Yasuyuki Igarashi Research Laboratory III (Immunology) Pharmaceuticals Research Division Mitsubishi Pharma Corporation Yokohama Japan Discovery Technology Laboratory Pharmaceuticals Research Division Mitsubishi Pharma Corporation Yokohama Japan Department of Biomembrane and Biofunctional Chemistry Graduate School of Pharmaceutical Sciences Hokkaido University Sapporo Japan

Sphingosine 1-phosphate （S1P）, a pleiotropic lysophospholipid, regulates signal transduction pathway via Gprotein-coupled receptors termed S1P1-5 in several types of the cells including lymphocytes. Higher levels of S1P4 mRNA as well as S1P1 mRNA are expressed in lymphoid tissues such as the spleen, thymus, lymph nodes, and Payer＇s patches. In contrast to S1P1 that plays an essential role in lymphocyte egress, little is known about the role of S1P4 in immune system. In this study, we found that S1P at 10 to 100 nM significantly induced the cell migration and the significant levels of S1P1 and S1P4 mRNA were expressed in mouse CD4 T cells, D10.G4.1 mouse Th2 cells, and ***-2 mouse thymoma cells. In D10.G4.1 and ***-2 cells, S1P-induced migration was almost completely inhibited by pretreatment with pertussis toxin, Clostoridium difficile toxin B, and （S）-enantiomer of FTY720-phosphate, a potent agonist at S1P1 and S1P4. The members of the Rho family small GTPase, Cdc42 and Rac were activated by S1P stimulation in these cells. The transfection with dominant negative or constitutively active forms of Cdc42 and Rac revealed that the activation of both Cdc42 and Rac is essential for S1P-induced migration of these cells. The immunoprecipitation assays using CHO cells co-expressing both S1P4 and S1P1 receptors indicated that S1P4 and S1P1 are associated on the cell surface. These results suggest that the association of S1P4 and S1PI plays an important role in migratory response of mouse T cells toward S1P.

关键词： S1Pi S1P4 S1P FTY720 T cell migration

Enlarged accessory spleen presenting stomach submucosal tumor

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World Journal of Gastroenterology 2007年第11期13卷 1752-1754页

作者： Shunzen Chin Hajime Isomoto Yohei Mizuta Chun-Yang Wen Saburo Shikuwa Shigeru kohno Second Department of Internal Medicine Nagasaki University School of Medicine 1-7-1 Sakamoto Nagasaki Japan Beihua University 3999 Huashan Road Jilin City

A 62-year-old man presented with upper abdominal discomfort underwent upper gastrointestinal endoscopy. Gastroscopy and endoscopic ultrasonography revealed a submucosal tumor (SMT) with homogenous echogenicity originated from extragastric organs. An abdominal contrast-enhanced computed tomography (CT) showed that the well-marginated ovoid mass, approximately 6 cm in diameter, enhanced homogenously to a similar degree as splenic parenchyma. 99mTechnetium sulfur colloid scintigraphy revealed the splenic nature of the mass. A diagnosis of accessory spleen mimicking gastric SMT was made. Subsequent follow-up was uneventful without performing splenectomy.

关键词： Accessory spleen ^99mTechnetium sulfur colloid scintigraphy Computed tomography Gastric submucosal tumor

High-grade pancreatic intraepithelial neoplasia diagnosed based on changes in magnetic resonance cholangiopancreatography findings:A case report

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World Journal of Clinical Cases 2024年第8期12卷 1487-1496页

作者： Nao Furuya Atsushi Yamaguchi Naohiro Kato Syuhei Sugata Takuro Hamada Takeshi Mizumoto Yuzuru Tamaru Ryusaku Kusunoki Toshio Kuwai Hirotaka Kouno Kazuya Kuraoka yoshiyuki Shibata Sho Tazuma Takeshi Sudo Hiroshi kohno Shiro Oka Department of Gastroenterology National Hospital Organization Kure Medical Center and Chugoku Cancer CenterKure 737-0023HiroshimaJapan Department of Gastroenterology Hiroshima University HospitalHiroshima 734-8551HiroshimaJapan Department of Pathology National Hospital Organization Kure Medical Center and Chugoku Cancer CenterKure 737-0023HiroshimaJapan Department of Surgery National Hospital Organization Kure Medical Center and Chugoku Cancer CenterKure 737-0023HiroshimaJapan

BACKGROUND High-grade pancreatic intraepithelial neoplasia(PanIN)exhibits no mass and is not detected by any examination ***,it can be diagnosed by pancreatic juice cytology from indirect *** previous cases were diagnosed based on findings of a focal stricture of the main pancreatic duct(MPD)and caudal MPD dilatation and subsequent pancreatic juice cytology using endoscopic retrograde cholangiopancreatography(ERCP).We experienced a case of high-grade PanIN with an unclear MPD over a 20-mm range,but without caudal MPD dilatation on magnetic resonance cholangiopancreatography(MRCP).CASE SUMMARY A 60-year-old female patient underwent computed tomography for a follow-up of uterine cancer post-excision,which revealed pancreatic *** revealed an unclear MPD of the pancreatic body at a 20-mm length without caudal MPD ***,course observation was *** 24 mo,MRCP revealed an increased caudal MPD caliber and a larger pancreatic *** performed ERCP and detected atypical cells suspected of adenocarcinoma by serial pancreatic juice aspiration cytology *** performed a distal pancreatectomy and obtained a histopathological diagnosis of high-grade *** parenchyma invasion was not observed,and curative resection was *** High-grade Pan-IN may cause MPD narrowing in a long range without caudal MPD dilatation.

关键词： Pancreatic cancer Pancreatic intraepithelial neoplasm High-grade pancreatic intraepithelial neoplasm Magnetic resonance cholangiopancreatography Carcinoma in situ Case report