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A sandwich ELISA kit reveals marked elevation of titin N-terminal fragment levels in the urine of mdx mice

Animal Models and Experimental Medicine 2022年第1期5卷 48-55页

作者： Taku Shirakawa Ayumu Ikushima Nobuhiro Maruyama yoshinori nambu Hiroyuki Awano Kayo Osawa Kei Nirasawa Yoichi Negishi Hisahide Nishio Shoji Fukushima Masafumi Matsuo Research Center for Locomotion Biology Kobe Gakuin UniversityKobeJapan KNC Department of Nucleic Acid Drug Discovery Faculty of RehabilitationKobe Gakuin UniversityKobeJapan Department of Pharmaceutics Faculty of Pharmaceutical SciencesKobe Gakuin UniversityKobeJapan Diagnostic&Research Reagents Division Immuno-Biological Laboratories Co.LtdFujiokaJapan Department of Pediatrics Kobe University Graduate School of MedicineKobeJapan Department of Medical Technology Faculty of Health SciencesKobe Tokiwa UniversityKobeJapan Department of Drug Delivery and Molecular Biopharmaceutics School of PharmacyTokyo University of Pharmacy and Life SciencesTokyoJapan

The mdx mouse is a model of Duchenne muscular dystrophy(DMD),a fatal progressive muscle wasting disease caused by dystrophin deficiency,and is used most widely in preclinical *** with dystrophin deficiency,however,show milder muscle strength phenotypes than *** human,the introduction of a sandwich enzyme-linked immunosorbent assay(ELISA)kit revealed a more than 700-fold increase in titin N-terminal fragment levels in the urine of pediatric patients with ***,the urinary titin level declines with aging,reflecting progression of muscle *** mouse,development of a highly sensitive ELISA kit has been ***,a sandwich ELISA kit to measure titin N-terminal fragment levels in mouse urine was *** developed kit showed good linearity,recovery,and repeatability in measuring recombinant or natural mouse titin N-terminal fragment *** titin N-terminal fragment concentration in the urine of mdx mice was more than 500-fold higher than that of normal *** titin was further analyzed by extending the collection of urine samples to both young(3-11 weeks old)and aged(56-58 weeks old)mdx *** concentration in the young group was significantly higher than that in the aged *** was concluded that muscle protein breakdown is active and persistent in mdx mice even though the muscle phenotype is *** results provide an opportunity to develop DMD treatments that aim to alleviate muscle protein breakdown by monitoring urinary titin levels.

关键词： biomarker Duchenne muscular dystrophy ELISA mdx mouse titin urine

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Direct peroral cholangioscopy using an ultraslim upper endoscope for biliary lesions

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Hepatobiliary & Pancreatic Diseases International 2014年第1期13卷 60-64页

作者： Shigefumi Omuta Iruru Maetani Takeo Ukita Tomoko nambu Katsushige Gon Hiroaki Shigoka yoshinori Saigusa Michihiro Saito Division of Gastroenterology Department of Internal Medicine Toho University Ohashi Medical Center

BACKGROUND: The development of direct peroral cholangioscopy(DPOC) using an ultraslim endoscope simplifies biliary cannulation. The conventional techniques are cumbersome to perform and require advanced skills. The recent introduction of the guidewires and balloons has improved the therapeutic outcomes. Here we describe an effective and easier method for performing DPOC using an ultraslim upper endoscope. METHODS: Indications for DPOC were the presence of stones on follow-up of patients who had previously undergone complete sphincteroplasty, including endoscopic sphincterotomy or endoscopic papillary large balloon dilatation. Fifteen patients underwent DPOC. An ultraslim endoscope was inserted perorally and was advanced into the major papilla. The ampulla of Vater was visualized by retroflexing the endoscope in the distal second portion of the duodenum, and then DPOC was performed using a wire-guided cannulation technique with an anchored intraductal balloon catheter. RESULTS: One patient failed in the treatment due to looping of the endoscope in the fornix of the stomach. Fourteen(93.3%) were successfully treated with our modified DPOC technique. Only one patient(6.7%) experienced an adverse event(pancreatitis) who responded well to conservative management. Residual stones of the common bile duct were completely removed in 3 patients. CONCLUSION: The modified method of DPOC is simple, safe and easy to access the bile duct.

关键词： direct peroral cholangioscopy ultraslim upper endoscope endoscopic retrograde cholangioscopy biliary tract

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