检索结果-南通市图书馆

Pathway and protein engineering for biosynthesis

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Synthetic and Systems Biotechnology 2022年第4期7卷 1044-1045页

作者： yongjin zhou Martin Grininger Hal Alper Dalian Institute of Chemical Physics Chinese Academy of SciencesChina Goethe University Frankfurt Germany The University of Texas at Austin USA

Sustainable biosynthesis of chemicals and efforts to create new molecules of interest require efficient enzymes and pathways as well as comprehensive tools and technologies to implement this *** are the key components for construction of efficient biosynthetic pathways,enzyme characterization and engineering can help to identify key enzymes and regulatory factors for construction of biosynthetic pathways,as well as improve enzyme performance;Pathway engineering can help construct biosynthetic pathways and balance metabolic network to improve biosynthetic efficiency;Tools and technologies facilitate the engineering of enzymes,pathways,and whole *** special issue focusing on“Pathway and Protein Engineering for Biosynthesis”comprises eight review articles and nine original research articles,which highlight and showcase current progress on Pathway and Protein Engineering and their application for biosynthesis.

关键词： ring engineering biosynthesis

Screening neutral sites for metabolic engineering of methylotrophic yeast Ogataea polymorpha

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Synthetic and Systems Biotechnology 2021年第2期6卷 63-68页

作者： Wei Yu Jiaoqi Gao Xiaoxin Zhai yongjin J.zhou Division of Biotechnology Dalian Institute of Chemical PhysicsChinese Academy of Sciences457 Zhongshan RoadDalian116023PR China CAS Key Laboratory of Separation Science for Analytical Chemistry Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian116023PR China Dalian Key Laboratory of Energy Biotechnology Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian116023PR China University of Chinese Academy of Sciences Beijing100049PR China

Methylotrophic yeast Ogataea polymorpha is capable to utilize multiple carbon feedstocks especially methanol as sole carbon source and energy,making it an ideal host for ***,the lack of gene integration sites limits its systems metabolic engineering,in particular construction of genome-integrated *** here screened the genomic neutral sites for gene integration without affecting cellular fitness,by genomic integration of an enhanced green fluorescent protein(eGFP)gene via CRISPR-Cas9 *** profiling the growth and fluorescent intensity in various media,17 genome positions were finally identified as potential neutral ***,integration of fatty alcohol synthetic pathway genes into neutral sites NS2 and NS3,enabled the production of 4.5 mg/L fatty alcohols,indicating that these neutral sites can be used for streamline metabolic engineering in *** can anticipate that the neutral sites screening method described here can be easily adopted to other eukaryotes.

关键词： Neutral sites Ogataea polymorpha Metabolic engineering CRISPR-Cas9 Fatty alcohol biosynthesis

An improved CRISPRi system in Pichia pastoris

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Synthetic and Systems Biotechnology 2023年第3期8卷 479-485页

作者： Shujing Qiao Fan Bai Peng Cai yongjin J.zhou Lun Yao Division of Biotechnology Dalian Institute of Chemical PhysicsChinese Academy of Sciences457 Zhongshan RoadDalian116023PR China CAS Key Laboratory of Separation Science for Analytical Chemistry Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian116023PR China Dalian Key Laboratory of Energy Biotechnology Dalian Institute of Chemical PhysicsCAS457 Zhongshan RoadDalian116023PR China University of Chinese Academy of Sciences Beijing100049China

CRISPR interference(CRISPRi)has been developed and widely used for gene repression in various *** we report an improved CRISPRi system in Pichia pastoris by fusing dCas9 with endogenous transcriptional repressor *** CRISPRi system shows strong repression of eGFP,with the highest efficiency of 85%.Repression of native genes is demonstrated by targeting AOX1 ***1 is efficiently repressed and the mutant strains show much slower growth in methanol *** of gRNA expression and processing on CRISPRi efficiency is also *** is found that gRNA processing by HH/HDV ribozymes or Csy4 endoribonuclease generating clean gRNA is critical to achieve strong repression,and Csy4 cleavage shows higher repression ***,gRNA expression using native tRNA transcription and processing systems results in relatively weaker repression of *** expression of two gRNAs targeting promoters of eGFP and AOX1 in an array together with Cys4 recognition sites,both genes can be repressed ***4-mediated gRNA array processing is further applied to repress fatty acyl-CoA synthetase genes(FAA1 and FAA2).Both genes are efficiently repressed,demonstrating that Cys4 endoribonuclease has the ability to cleave gRNAs array and can be can be used for multiplexed gene repression in ***.

关键词： Pichia pastoris CRISPRi HH/HDV Csy4 gRNA array

Engineering yeast for bio-production of food ingredients

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Systems Microbiology and Biomanufacturing 2023年第1期3卷 2-11页

作者： Chunyang Cao Jiaoqi Gao Beiwei Zhu yongjin J.zhou Division of Biotechnology Dalian Institute of Chemical PhysicsChinese Academy of Sciences457 Zhongshan RoadDalian 116023People’s Republic of China National Engineering Research Center of Seafood Collaborative Innovation Center of Seafood Deep ProcessingLiaoning Province Collaborative Innovation Center for Marine Food Deep ProcessingSchool of Food Science and TechnologyDalian Polytechnic UniversityDalian 116034People’s Republic of China

The provision of an adequate and high-quality food supply is a challenging issue due to the continued growth of the population and the reduction of arable land *** solve these problems,new and efficient food manufacturing processes need to be developed to meet the needs for healthy and nutritious *** engineering of microorganisms is a feasible approach to alleviate this problem due to its efficient *** thousands of years,yeast has been used as a cell factory for manufacturing bread,beer and *** the development of synthetic biology expands its ability for synthesis of food ingredients,fuels,pharmaceuticals and chemical *** mini review focuses on metabolic engineering of yeast cell factories to synthesize compounds that have been used as food ingredients with highlighting four food flavors.

关键词： Yeast Bio-products Metabolic engineering Food ingredients Cell factory

Harnessing sub-organelle metabolism for biosynthesis of isoprenoids in yeast

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Synthetic and Systems Biotechnology 2020年第3期5卷 179-186页

作者： Xuan Cao Shan Yang Chunyang Cao yongjin J.zhou Division of Biotechnology Dalian Institute of Chemical PhysicsChinese Academy of Sciences457 Zhongshan RoadDalian116023PR China CAS Key Laboratory of Separation Science for Analytical Chemistry Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian116023PR China Dalian Key Laboratory of Energy Biotechnology Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian116023PR China University of Chinese Academy of Sciences Beijing100049PR China School of Food Science and Technology Dalian Polytechnic UniversityDalian116034PR China

Current yeast metabolic engineering in isoprenoids production mainly focuses on rewiring of cytosolic metabolic ***,the precursors,cofactors and the enzymes are distributed in various sub-cellular compartments,which may hamper isoprenoid *** the other side,pathway compartmentalization provides several advantages for improving metabolic flux toward target *** here summarize the recent advances on harnessing sub-organelle for isoprenoids biosynthesis in yeast,and analyze the knowledge about the localization of enzymes,cofactors and metabolites for guiding the rewiring of the sub-organelle *** review may provide some insights for constructing efficient yeast cell factories for production of isoprenoids and even other natural products.

关键词： Isoprenoids Yeast Compartmentalization Sub-organelle metabolism

Construction of microbial chassis for terpenoid discovery

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Synthetic and Systems Biotechnology 2022年第4期7卷 1181-1182页

作者： Xiaoxin Zhai Lun Yao yongjin J.zhou Dalian Institute of Chemical Physics Chinese Academy of SciencesDalian116023China CAS Key Laboratory of Separation Science for Analytical Chemistry Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian116023China Dalian Key Laboratory of Energy Biotechnology Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian116023China

Natural products are one of the major sources of small-molecule pharmaceutical drugs[1,2].However,discovery of novel natural products to expand the pool of potential drug candidates is becoming more and more *** of the traditional molecule discovery methods are based on direct separation of natural product from its natural hosts or homology search of biosynthetic ***,there are currently two main obstacles in discovery of natural products from their native hosts:1)the difficulty in cultivation of the native hosts under laboratory conditions,2)no or not enough production of target natural products due to the silence of the biosynthetic pathway genes in the native ***,novel platforms are required for efficient discovery of new natural products.

关键词： becoming enough separation

Promoter engineering enables precise metabolic regulation towards efficientβ-elemene production in Ogataea polymorpha

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Synthetic and Systems Biotechnology 2024年第2期9卷 234-241页

作者： Min Ye Jiaoqi Gao Jingjing Li Wei Yu Fan Bai yongjin J.zhou Division of Biotechnology Dalian Institute of Chemical PhysicsChinese Academy of Sciences457 Zhongshan RoadDalian116023PR China CAS Key Laboratory of Separation Science for Analytical Chemistry Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian116023PR China Dalian Key Laboratory of Energy Biotechnology Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian116023PR China University of Chinese Academy of Sciences Beijing100049PR China

Precisely controlling gene expression is beneficial for optimizing biosynthetic pathways for improving the ***,promoters in nonconventional yeasts such as Ogataea polymorpha are always limited,which results in incompatible gene ***,we expanded the promoter library in *** based on transcriptional data,among which 13 constitutive promoters had the strengths ranging from 0–55%of PGAP,the commonly used strong constitutive promoter,and 2 were growth phase-dependent ***,2 hybrid growth phase-dependent promoters were constructed and characterized,which had 2-fold higher ***,promoter engineering was applied to precisely regulate cellular metabolism for efficient production ofβ-*** glyceraldehyde-3-phosphate dehydrogenase gene GAP was downregulated to drive more flux into pentose phosphate pathway(PPP)and then to enhance the supply of acetyl-CoA by using phosphoketolase-phosphotransacetylase(PK-PTA)*** with the phase-dependent expression of synthase module(ERG20∼LsLTC2 fusion),the highest titer of 5.24 g/L with a yield of 0.037 g/(g glucose)was achieved in strain YY150U under fed-batch fermentation in shake *** work characterized and engineered a series of promoters,that can be used to fine-tune genes for constructing efficient yeast cell factories.

关键词： Promoter library Growth phase-dependent promoters Promoter engineering Precise metabolic regulation Ogataea polymorpha

Automated analysis of pectoralis major thickness in pec-fly exercises:evolving from manual measurement to deep learning techniques

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Visual Computing for Industry,Biomedicine,and Art 2024年第1期7卷 329-339页

作者： Shangyu Cai Yongsheng Lin Haoxin Chen Zihao Huang yongjin zhou Yongping Zheng School of Biomedical Engineering Medical SchoolShenzhen UniversityShenzhen 518073China Department of Biomedical Engineering the Hong Kong Polytechnic UniversityHong Kong 999077China

This study addresses a limitation of prior research on pectoralis major(PMaj)thickness changes during the pectoralis fly exercise using a wearable ultrasound imaging *** previous studies used manual measurement and subjective evaluation,it is important to acknowledge the subsequent limitations of automating widespread *** then employed a deep learning model for image segmentation and automated measurement to solve the problem and study the additional quantitative supplementary information that could be *** results revealed increased PMaj thickness changes in the coronal plane within the probe detection region when real-time ultrasound imaging(RUSI)visual biofeedback was incorporated,regardless of load intensity(50%or 80%of one-repetition maximum).Additionally,participants showed uniform thickness changes in the PMaj in response to enhanced RUSI ***,the differences in PMaj thickness changes between load intensities were reduced by RUSI biofeedback,suggesting altered muscle activation *** identified the optimal measurement location for the maximal PMaj thickness close to the rib end and emphasized the lightweight applicability of our model for fitness training and muscle *** studies can refine load intensities,investigate diverse parameters,and employ different network models to enhance *** study contributes to our understanding of the effects of muscle physiology and exercise training.

关键词： B-mode ultrasound Deep learning Exercise training Pectoralis major Wearable ultrasound-imaging biofeedback

Genetic tools for metabolic engineering of Pichia pastoris

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Engineering Microbiology 2023年第4期3卷 59-66页

作者： Xiaoyan Wu Peng Cai Lun Yao yongjin J zhou Division of Biotechnology Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China Dalian Key Laboratory of Energy Biotechnology Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China University of Chinese Academy of Sciences Beijing 100049China CAS Key Laboratory of Separation Science for Analytical Chemistry Dalian Institute of7 Chemical PhysicsChinese Academy of SciencesDalian 116023China

The methylotrophic yeast Pichia pastoris(also known as Komagataella phaffii)is widely used as a yeast cell factory for producing heterologous ***,it has gained attention for its potential in producing chemicals from inexpensive feedstocks,which requires efficient genetic engineering *** review provides an overview of the current advances in developing genetic tools for metabolic engineering of *** topics cover promoters,terminators,plasmids,genome integration sites,and genetic editing systems,with a special focus on the development of CRISPR/Cas systems and their comparison to other genome editing ***,this review highlights the prospects of multiplex genome integration,fine-tuning gene expression,and single-base editing ***,the aim of this review is to provide valuable insights into current genetic engineering and discuss potential directions for future efforts in developing efficient genetic tools in ***.

关键词： Pichia pastoris Genetic tools Metabolic engineering CRISPR/Cas9