检索结果-南通市图书馆

Development of RPA-Cas12a-fluorescence assay for rapid and reliable detection of human bocavirus 1

在线全文

学校读者我要写书评

暂无评论

Animal Models and Experimental Medicine 2024年第2期7卷 179-188页

作者： Weidong Qian Xuefei Wang Ting Wang Jie Huang Qian Zhang yongdong li Si Chen School of Food and Biological Engineering Shaanxi University of Science and TechnologyXi'anP.R.China Department of Dermatology Huazhong University of Science and Technology Union Shenzhen HospitalShenzhenP.R.China Ningbo Municipal Center for Disease Control and Prevention NingboP.R.China Medical College of Shenzhen University ShenzhenP.R.China

Human bocavirus(HBoV)1 is considered an important pathogen that mainly affects infants aged 6–24 months,but preventing viral transmission in resource-limited regions through rapid and affordable on-site diagnosis of individuals with early infection of HBoV1 remains somewhat ***,we present a novel faster,lower cost,reliable method for the detection of HBoV1,which integrates a recombinase polymerase amplification(RPA)assay with the CRISPR/Cas12a system,designated the RPA-Cas12a-fluorescence *** RPA-Cas12a-fluorescence system can specifically detect target gene levels as low as 0.5 copies of HBoV1 plasmid DNA per microliter within 40 min at 37℃without the need for sophisticated *** method also demonstrates excellent specificity without cross-reactivity to non-target ***,the method was appraised using 28 clinical samples,and displayed high accuracy with positive and negative predictive agreement of 90.9%and 100%,***,our proposed rapid and sensitive HBoV1 detection method,the RPA-Cas12a-fluorescence assay,shows promising potential for early on-site diagnosis of HBoV1 infection in the fields of public health and health *** established RPA-Cas12a-fluorescence assay is rapid and reliable method for human bocavirus 1 *** RPA-Cas12a-fluorescence assay can be completed within 40 min with robust specificity and sensitivity of 0.5 copies/μl.

关键词： CRISPR-Cas12a detection human bocavirus 1 on-site diagnosis recombinase polymerase amplification

在线全文

学校读者我要写书评

暂无评论

一种基于耦合电感的高增益软开关谐振变换器

电工技术学报 2024年第1期39卷 194-205页

作者：王哲李驰郑泽东李永东电力系统及发电设备控制和仿真国家重点实验室(清华大学) 北京100084

该文提出了一种具有高增益、软开关的新型谐振变换器。该谐振变换器使用耦合电感来提高电压增益,升压比不仅取决于占空比,还取决于耦合电感的变比,具有高增益的优点。此外,由于采用了有源钳位技术,漏感中的能量可以被回收利用,用来对开... 详细信息

该文提出了一种具有高增益、软开关的新型谐振变换器。该谐振变换器使用耦合电感来提高电压增益,升压比不仅取决于占空比,还取决于耦合电感的变比,具有高增益的优点。此外,由于采用了有源钳位技术,漏感中的能量可以被回收利用,用来对开关管的结电容充放电提供能量,从而实现软开关。由于采用了耦合电感,所提出的变换器可以在较低的开关管电压应力的情况下实现较高的输出电压,因此可以使用低导通电阻的低压器件,从而提升系统的效率。该文分析了该谐振变换器的工作原理,并推导了输出电压、关断电流应力等参数的解析表达式。在此基础上,从理论上分析了该变换器取得软开关的条件,并对该变换器的各个器件的电压电流应力进行分析,为器件选型提供了理论依据。最后,搭建1kW实验样机,针对该文提出的基于耦合电感的谐振变换器的高增益、软开关、低电压应力、高效率等性能进行了实验验证。通过实验得出所提出的拓扑可以在10倍增益的情况下达到最高97.5%的效率,表明所提拓扑的优越性。

关键词：耦合电感谐振变换器高增益软开关

在线全文

学校读者我要写书评

暂无评论

“两墙合一”深基坑工程设计与技术创新

岩土工程技术 2024年第4期38卷 384-390页

作者：李伟李永东北京市勘察设计研究院有限公司北京100038

北京CBD核心区某项目基坑深度36m,因受场地空间限制,采用“两墙合一”+内支撑支护体系,在实现基坑挡土、止水功能的同时完成了地下结构外墙施工。为提高工程质量和施工效率,针对地连墙与主体结构的连接、槽段接头处理方式、有限空间导... 详细信息

北京CBD核心区某项目基坑深度36m,因受场地空间限制,采用“两墙合一”+内支撑支护体系,在实现基坑挡土、止水功能的同时完成了地下结构外墙施工。为提高工程质量和施工效率,针对地连墙与主体结构的连接、槽段接头处理方式、有限空间导墙结合近接建筑物预换撑等方面进行了技术创新,取得了较好的工程效果,可供类似工程参考。

关键词：两墙合一预埋件连接槽段接头处理异形导墙兼预换撑结构支撑兼做栈桥

Mesozoic uplift of the Dabashan and Micangshan-Hannan Dome in the South Qinling orogenic belt

维普期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

Science China Earth Sciences 2022年第3期65卷 426-436页

作者： yongdong li Xiong XIONG Yashan FENG School of Geophysics and Geomatics University of GeosciencesWuhan 430074China

The topographic evolution of continental orogens is important for understanding continental orogenic processes,geodynamic mechanisms,and climatic and environmental *** Qinling Orogen is a major orogenic belt in China,and its uplift history can provide insights into the tectonic configuration and geodynamics of China and East *** studies have shown that the Dabashan and Micangshan-Hannan Dome(MHD)in the South Qinling orogenic belt were uplifted during the ***,the magnitude of the uplift remains *** this study,using sedimentary records in the northern Sichuan Basin and lithospheric flexural modeling,we estimated the magnitude of Mesozoic uplift of the Dabashan and MHD,along with the effective elastic thickness(Te)of the Sichuan *** Dabashan and MHD were uplifted by approximately 1220 and 880 m during the Middle Jurassic and Early Cretaceous,***,we propose that the present-day elevation of the Dabashan and MHD is primarily the result of Mesozoic *** differences in the duration and amount of uplift between different tectonic units indicate that the uplift processes and driving mechanisms in the South Qinling orogenic belt were different in the Mesozoic and *** uplift was the result of convergence of the North China and South China blocks advanced from east to west,whereas Cenozoic uplift was driven by ongoing indentation of the Indian Plate into Eurasia from southwest to *** lithospheric strength of the northern Sichuan Basin was weakened from the Middle Jurassic to Early Cretaceous,and Tedecreased from 73 to 57 *** may have been caused by the flexure-related bending stresses in the lithosphere that developed due to the large topographic loading.

关键词： South Qinling orogenic belt Dabashan Micangshan-Hannan Dome Mesozoic Uplift

在线全文

学校读者我要写书评

暂无评论

“作家”身份的生成与新文学的发生

东方论坛（青岛大学学报） 2024年第3期 83-95页

作者：包啟飞李永东西南大学文学院重庆400715

“谁是作家”“作家是什么”并非不言自明的问题,而是关涉到五四时期新文学倡导者有意建构的一套身份与价值观念体系。经由从“著作家”到“创作家”、从“文人”到“作家”的指称转换,以及现代文坛对超人情感和现代知识的强调,作家被... 详细信息

“谁是作家”“作家是什么”并非不言自明的问题,而是关涉到五四时期新文学倡导者有意建构的一套身份与价值观念体系。经由从“著作家”到“创作家”、从“文人”到“作家”的指称转换,以及现代文坛对超人情感和现代知识的强调,作家被赋予“感化读者”与“改进国家”的社会职能。“作家”的生成与判定有赖于现代读者的“发明”与现代文学机制的助推。现代“作家”身兼报刊编辑、文学批评家等多重身份,怀有“启蒙”与“救亡”的价值追求,带来了文学创作主体的现代转换。“作家”身份的生成与塑造反映了新文学发生的内在逻辑,也提供了一条反思当下作家观的有效途径。

关键词：作家新文学的发生著作家文人创作主体

维普期刊数据库博看期刊

The “liberation” Theme of Modern Chinese literature

在线全文

学校读者我要写书评

暂无评论

Social Sciences in China 2022年第1期43卷 21-36页

作者： li yongdong 邵亚楠 School of literature Southwestern University the School of Foreign Languages at Tianjin Chengjian University

The concept of “liberation” has profoundly influenced the development of modern Chinese literature,giving rise to a “liberation” theme with profound *** Chinese literature can be analyzed from the joint perspectives of the liberators,the routes to liberation and the scope of *** among the liberators have led to the two different concepts of self-liberation and being liberated;the differences in the routes to liberation have led to the intermixture of liberation based on persuasion and struggle-based liberation;and the difference in the scope of liberation derives from the alternation of the partial achievement of thoroughgoing liberation and the full realization of incremental *** Chinese concept of liberation was strongly shaped by the Communist Party of China (CPC);compared to the work of those writers influenced by the CPC’s concept of “liberation,” the writings of ordinary non-Communist writers present “liberation” *** latter tend to adopt an either/or attitude toward liberation,whereas the former ensure that liberation theme reflects the interaction and blending of different types of “liberation” concepts,thus establishing a new form of “liberation” *** mark the centenary of the CPC,we have reviewed and explored the “liberation” theme of modern Chinese literature in the hope that we will gain a full understanding of the theoretical discourse and historical practice of “liberation,” taking a dialectical approach to freedom and responsibility,respecting tradition while boldly pursuing innovation,and striving to explore the realistic significance and ideological value of literary works.

关键词： modern Chinese literature “liberation”theme Communist Party of China concept of liberation

在线全文

学校读者我要写书评

暂无评论

我国芝麻田杂草防控现状及建议

植物保护 2024年第3期50卷 10-16,45页

作者：刘新涛赵辉倪云霞赵新贝王婧李永冬刘红彦河南省农业科学院植物保护研究所农业农村部华北南部作物有害生物综合治理重点实验室河南省农作物病虫害防治重点实验室郑州450002

本研究对我国主要芝麻产区杂草发生情况和防控现状进行了调查。结果显示,我国芝麻田杂草共计31科86种,使用的除草剂共15种,普遍存在阔叶杂草难防控等问题。芝麻田杂草防控难与科研基础薄弱和可用除草剂品种严重不足有关。针对上述情况,... 详细信息

本研究对我国主要芝麻产区杂草发生情况和防控现状进行了调查。结果显示,我国芝麻田杂草共计31科86种,使用的除草剂共15种,普遍存在阔叶杂草难防控等问题。芝麻田杂草防控难与科研基础薄弱和可用除草剂品种严重不足有关。针对上述情况,提出加大基础研究力度、重视除草剂筛选登记工作、加强抗除草剂育种、加速植保机械的研发及产业化、研究和推广注重芝麻田生态系统保护的标准化防控技术等建议。

关键词：芝麻杂草防控现状建议

同方期刊数据库博看期刊

在线全文

学校读者我要写书评

暂无评论

激光诱导击穿光谱仪器化研制综述

量子电子学报 2024年第3期41卷 399-420页

作者：蒲磊邱岩卢博文朱斌梅金娜蔡振吴坚李兴文李永东杭玉桦西安交通大学电子物理与器件教育部重点实验室陕西西安710049 苏州热工研究院有限公司江苏苏州215004 西安交通大学电力设备电气绝缘国家重点实验室陕西西安710049

作为一种新型物质探测技术,激光诱导击穿光谱(liBS)技术因具有制样简单、非接触式测量、现场适应能力强、分析速度快以及能同时对多种元素进行识别和定量分析等突出优点,近年来在工厂运维、垃圾回收、岩矿分析、文化遗产保护、环境监测... 详细信息

作为一种新型物质探测技术,激光诱导击穿光谱(liBS)技术因具有制样简单、非接触式测量、现场适应能力强、分析速度快以及能同时对多种元素进行识别和定量分析等突出优点,近年来在工厂运维、垃圾回收、岩矿分析、文化遗产保护、环境监测、生物医疗、食品检验、国土安全等诸多领域得到广泛应用。在从实验室逐步走向实际应用的过程中,各国研究人员开发了各式liBS仪器,根据其大小和使用特点,大致可分为台式、远程式和便携式三类。本研究对激光诱导击穿光谱的原理、系统构成和发展脉络作了系统性的介绍,对现有的liBS仪器进行了分类与综述,细致探讨了各类设备的优势及面临的挑战,并对未来的发展方向进行了展望。

关键词：激光技术激光诱导击穿光谱仪器研制定性定量分析

维普期刊数据库博看期刊

Bifunctional two-carbon reagent made from acetylene via 1,2-difunctionalization and its applications

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 2024年第3期67卷 936-944页

作者： Bo Yang Kangkui li yongdong Wang Shifa Zhu Key Laboratory of Functional Molecular Engineering of Guangdong Province School of Chemistry and Chemical EngineeringSouth China University of TechnologyGuangzhou 510640China School of Chemistry and Chemical Engineering Zhejiang Sci-Tech UniversityHangzhou 310018China Singfar Laboratories Guangzhou 510670China

There are very limited approaches to directly gluing two molecules for the production of internal alkenes using the vastly abundant acetylene via 1,2-difunctionalization. Conversion of gaseous acetylene to internal alkenes via 1,2-difunctionalization in a desired manner is not as easy as it might be expected due to the potential competition reactions between acetylene and alkene produced and the difficulty in handling this harmful reagent and controlling the regio-and stereoselectivity. In this work, we designed an efficient catalytic system for the incorporation of acetylene gas into tremendous(E)-β-bromo vinylsulfones, which are bench-stable, easy to operate, and can function as bifunctional acetylene and show a rich reactivity profile in Sonogashira coupling, Heck coupling, substituted reaction, and various desulfonylation transformations, providing numerous internal alkenes.

关键词： acetylene 1 2-difunctionalization photocatalysis vinylsulfone