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Employing pH-responsive RNA triplex to control CRISPR/Cas9-mediated gene manipulation in mammalian cells

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Chinese Chemical Letters 2024年第9期35卷 219-222页

作者： Yan Liu Yang Wang Jiayi Zhu Xuxian Su Xudong Lin liang Xu Xiwen xing Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument School of Biomedical EngineeringShenzhen Campus of Sun Yat-sen UniversitySun Yat-sen UniversityShenzhen 518107China MOE Key Laboratory of Bioinorganic and Synthetic Chemistry School of ChemistrySun Yat-sen UniversityGuangzhou 510275China Department of Cardiology the first people’s hospital of FoshanFoshan 528000China Department of Biotechnology College of Life Science and TechnologyJinan UniversityGuangzhou 510632China

The clustered regularly interspersed short palindromic repeats/CRISPR-associated protein 9(CRISPR/Cas9)system is an RNA-guided platform for highly efficient and specific genome targeting in diverse organisms,which has been exploited for various applications in gene *** with the constantly active CRISPR/Cas9 function,conditional control of its activity can improve the performance of the system with reduced side effects and high spatiotemporal *** pH-responsive triplex RNA was successful used in CRISPR-derived RNA/trans-activating crRNA(crRNA/tracrRNA)of CRISPR/Cas9,thus affecting RNA/dead Cas9(dCas9)complex to target DNA in vitro and in *** design of triplex RNA opens a new window towards the broad involvement of eukaryotic cells for conditional control of CRISPR/Cas9function.?2024 Published by Elsevier *** behalf of Chinese Chemical Society and Institute of Materia Medica,Chinese Academy of Medical Sciences.

关键词： RNA triplex pH-responsive CRISPR/Cas9 Gene manipulation Eukaryotic cells

Concerted Steric and Electronic Strategy in Thermostable Salicylaldiminato Nickel Catalysts for Ethylene(Co)polymerization

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Chinese Journal of Polymer Science 2024年第8期42卷 1085-1092页

作者： Hong-Yu Ji Hong-liang Mu Chun-Feng Tang Yu-xing Zhang Yue Chi Zhong-Bao Jian Key Laboratory of Advanced Structural Materials Ministry of EducationSchool of Materials Science and EngineeringChangchun University of TechnologyChangchun 130012China State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China

Olefin polymerization is one of the most im portant chemical reactions in *** work presents a strategy that emphasizes the synergistic meta/poro-steric hindrance of N-aryl groups and electronic effects in newly synthesized neutral salicylaldiminato nickel *** nickel(Ⅱ)catalysts exhibit exceptional thermostability,ranging from 30℃to 130℃,demonstrating enhanced catalytic activities and broadly regulated polyethylene molecular weights(3-341 kg·mol^(-1))and controlled polymer branch density(2-102 brs/1000C).The preferred catalyst Ni3 with concerted steric and electronic effects enables the production of solid-state semi-crystalline polyethylene materials at temperatures below 90℃.Notably,Ni3 exhibits an impressive tolerance of 110℃and can withstand even the challenging polymerization temperature of 130℃,leading to the production of polyethylene wax and ***,functionalized polyethylene is produced.

关键词： Polyolefin Salicylaldiminato ligand Nickel catalyst Oil Wax

In-situ interfacial passivation and self-adaptability synergistically stabilizing all-solid-state lithium metal batteries

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Journal of Energy Chemistry 2024年第1期88卷 282-292,I0007页

作者： Huanhui Chen xing Cao Moujie Huang Xiangzhong Ren Yubin Zhao liang Yu Ya Liu Liubiao Zhong Yejun Qiu Shenzhen Engineering Lab of Flexible Transparent Conductive Films School of Materials Science and EngineeringHarbin Institute of TechnologyShenzhen 518055GuangdongChina Guangdong Provincial Key Laboratory of Semiconductor Optoelectronic Materials and Intelligent Photonic Systems School of Materials Science and EngineeringHarbin Institute of TechnologyShenzhen 518055GuangdongChina College of Chemistry and Environmental Engineering Shenzhen UniversityShenzhen 518060GuangdongChina School of Resource and Environmental Sciences Wuhan UniversityWuhan 430072HubeiChina

The function of solid electrolytes and the composition of solid electrolyte interphase(SEI)are highly significant for inhibiting the growth of Li ***,we report an in-situ interfacial passivation combined with self-adaptability strategy to reinforce Li_(0.33)La_(0.557)TiO_(3)(LLTO)-based solid-state ***,a functional SEI enriched with LiF/Li_(3)PO_(4) is formed by in-situ electrochemical conversion,which is greatly beneficial to improving interface compatibility and enhancing ion *** the polarized dielectric BaTiO_(3)-polyamic acid(BTO-PAA,BP)film greatly improves the Li-ion transport kinetics and homogenizes the Li *** expected,the resulting electrolyte offers considerable ionic conductivity at room temperature(4.3 x 10~(-4)S cm^(-1))and appreciable electrochemical decomposition voltage(5.23 V)after electrochemical *** Li-LiFePO_(4) batteries,it shows a high specific capacity of 153 mA h g^(-1)at 0.2C after 100 cycles and a long-term durability of 115 mA h g^(-1)at 1.0 C after 800 ***,a stable Li plating/stripping can be achieved for more than 900 h at 0.5 mA cm^(-2).The stabilization mechanisms are elucidated by ex-situ XRD,ex-situ XPS,and ex-situ FTIR techniques,and the corresponding results reveal that the interfacial passivation combined with polarization effect is an effective strategy for improving the electrochemical *** present study provides a deeper insight into the dynamic adjustment of electrode-electrolyte interfacial for solid-state lithium batteries.

关键词： Solid-state lithium batteries Composite solid electrolyte In-situ polymerization Interfacial passivation layer Self-adaptability

Two-dimensional conjugated coordination polymer monolayer as anode material for lithium-ion batteries:A DFT study

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Chinese Chemical Letters 2024年第6期35卷 180-184页

作者： Xin-Tong Zhao Jin-Zhi Guo Wen-liang Li Jing-Ping Zhang xing-Long Wu MOE Key Laboratory for UV Light-Emitting Materials and Technology Northeast Normal UniversityChangchun 130024China Faculty of Chemistry Northeast Normal UniversityChangchun 130024China Key Laboratory of Organo-Pharmaceutical Chemistry of Jiangxi Province Gannan Normal UniversityGanzhou 341000China

Nowadays,lithium-ion batteries(LIBs)play a crucial role in modern society in the aspect of portable electronic devices and large-scale smart ***,the current performance of lithium-ion batteries has been unable to meet the growing expectations of society and scientific ***,we have synthetically investigated availability of 2D Ni-TABQ monolayer as anode based on DFT for LIBs *** findings have demonstrated that 2D Ni-TABQ monolayer is a semiconductor with a small band gap of 0.2 eV,which suggest that the electronic property of 2D Ni-TABQ monolayer would take place an evident shift from semiconductor property to metallic property after Li ***,we checked the stability of 2D Ni-TABQ monolayer and investigated the viability of exfoliation from bulk multilayer Ni-TABQ to form 2D Ni-TABQ monolayer in the light of exfoliation energy and binding *** continuously studied electrochemical properties of 2D Ni-TABQ monolayer with respect of theoretical specific capacity,Li-ion diffusion barriers and open-circuit *** the charging process,2D Ni-TABQ monolayer can achieve a high specific capacity of 722 m Ah/g with an open-circuit voltage range from 1.12 V to 0.22 *** aforementioned results make the 2D Ni-TABQ monolayer a promising anode for LIBs.

关键词： The first principles Density functional theory Lithium-ion battery Anode material Two-dimensional material

Triggering triplet excitons of carbon nanodots through nanospace domain confinement for multicolor phosphorescence in aqueous solution

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Nano Research 2024年第7期17卷 6534-6543页

作者： Ya-Chuan liang Qing Cao Yuan Deng yong Wang Kai-Kai Liu Chong-Xin Shan School of Electronics and Information Zhengzhou University of Light IndustryZhengzhou 450002China Henan Key Laboratory of Diamond Optoelectronic Material and Devices Key Laboratory of Material PhysicsMinistry of EducationSchool of Physics and MicroelectronicsZhengzhou UniversityZhengzhou 450001China Academy for Quantum Science and Technology Zhengzhou University of Light IndustryZhengzhou 450002China Institute of Quantum Materials and Physics Henan Academy of SciencesZhengzhou 450046China

Easy non-radiative decay property of long-lived triplet excitons in aqueous solution obstructs their applications in aquatic *** reported phosphorescence phenomena in aqueous solution have excited researchers enormously but achieving full-color water-soluble phosphorescent carbon nanodots(CNDs)is still a challenging ***,full-color phosphorescence of water-soluble CNDs has been demonstrated by triggering their triplet excitons through nanospace domain confinement,and Förster energy resonance transfer is used for further tuning phosphorescence *** phosphorescence spans across most of the visible spectrum,ranging from 400 to 700 *** an aqueous solution,the CNDs exhibits blue,green,and red phosphorescence,lasting for approximately 6,10,and 7 s,***,the phosphorescence quantum yields are 11.85%,8.6%and 3.56%,making them readily discernible to the naked eyes and laying a solid foundation for practical ***,phosphorescence flexible optical display and bioimaging have been demonstrated by using the multicolor CNDs-based nanomaterials,showing distinct superiority for accuracy and complete display and imaging in complex emission background.

关键词： carbon nanodots triplet excitons multicolor aqueous solution

DMF-scMT-seq linking methylome and transcriptome within single cells with digital microfluidics

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Science China Chemistry 2024年第6期67卷 2070-2078页

作者： xing Xu Xi Zeng Xin Lin Shiyan Lin Shanshan liang Tian Tian Rui Su Jia Song Chaoyong Yang Collaborative Innovation Center of Chemistry for Energy Materials The MOE Key Laboratory of Spectrochemical Analysis and InstrumentationState Key Laboratory of Physical Chemistry of Solid SurfacesDepartment of Chemical BiologyCollege of Chemistry and Chemical EngineeringThe First Affiliated Hospital of Xiamen UniversityXiamen UniversityXiamen361005China Institute of Molecular Medicine Renji HospitalShanghai Jiao Tong University School of MedicineShanghai200127China Chemistry and Materials Science College Shanghai Normal UniversityShanghai200234China Chemistry and Biomedicine Innovation Center(ChemBIC) School of Chemistry and Chemical EngineeringNanjing UniversityNanjing210023China

Single-cell joint analysis of methylome and transcriptome reveals how the methylation regulates the transcriptional ***,traditional bench-top protocols for single-cell DNA methylation and RNA transcription co-detection are laborintensive,cost-ineffective and ***,we establish the DMF-sc MT-seq,a highly-efficient and cost-effective method to simultaneously analyze single-cell DNA methylation and transcriptional activity based on digital *** MT-seq automates the workflow of single-cell isolation,cellular hypotonic lysis,nucleic acid separation and methylome/transcriptome library construction in a contactless and addressable *** system ensures high accuracy(R>0.85),high gene detection ability(14,697 genes per cell at 4 million sequencing depth),and high CpG coverage(677,198 CpG sites per cell at 1million sequencing depth).By using DMF-sc MT-seq,the relationship of DNA methylation and RNA transcription under different genomic contexts is *** further apply DMF-sc MT-seq to study the dynamics of transcription regulation with methylation-inhibiting anti-tumor Decitabine,and identify the methylated promoter/gene body driven genes in response to Decitabine ***-sc MT-seq facilitates the construction of the correlation of DNA methylation and transcriptional activity at the single-cell level in a flexible,sensitive and accurate way,which is anticipated to be a powerful tool in studying single-cell biological systems.

关键词： single-cell analysis digital microfluidics methylome sequencing transcriptome sequencing

AquaticTox:A Web-Based Tool for Aquatic Toxicity Evaluation Based on Ensemble Learning to Facilitate the Screening of Green Chemicals

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Environment & Health 2024年第4期2卷 202-211页

作者： xing-xing Shi Zhi-Zheng Wang Yu-liang Wang Fan Wang Guang-Fu Yang National Key Laboratory of Green Pesticide Central China Normal UniversityWuhan 430079China

The widespread use of chemical products inevitably brings many side effects as environmental *** assessment of compounds to aquatic life plays an important role in protecting the environment from their ***,in vivo animal testing approaches for aquatic toxicity evaluation are timeconsuming,expensive,and ethically limited,especially when there are a great number of *** silico modeling methods can effectively improve the toxicity evaluation efficiency and save ***,we present a web-based server,AquaticTox,which incorporates a series of ensemble models to predict acute toxicity of organic compounds in aquatic organisms,covering Oncorhynchus mykiss,Pimephales promelas,Daphnia magna,Pseudokirchneriella subcapitata,and Tetrahymena *** predictive models are built through ensemble learning algorithms based on six base *** ensemble models outperform all corresponding single models,achieving area under the curve(AUC)scores of 0.75−*** to the best single models,the average precisions of the ensemble models have been increased by 12−22%.Additionally,a self-built knowledge base of the structure-aquatic toxic mode of action(MOA)relationship was integrated into AquaticTox for toxicity mechanism ***,the user-friendly tool(https://***/ccb/server/AquaticTox);could facilitate the identification of aquatic toxic chemicals and the design of green molecules.

关键词： ecotoxicity aquatic toxicity water environment protection toxicity prediction structure−toxicity relationship deep learning

Orientational growth behavior and mechanism of delta(δ)phase precipitation in cold-rolled Inconel 718 alloy during heat treatment

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Journal of Iron and Steel Research International 2024年第1期31卷 264-274页

作者： Neng-yong Ye Guang-liang Zhang Tian-yue Huang Ming Cheng Shi-hong Zhang Lei Wang School of Materials Science&Engineering Taizhou UniversityTaizhou318000ZhejiangChina Institute of Metal Research Chinese Academy of SciencesShenyang110016LiaoningChina

Due to the important role ofδphase's quantity and morphology in the mechanical and fatigue properties of Inconel 718 alloy and its components,it is necessary to renew insights into the effect of cold deformation on theδphase precipitation,especially on the morphology ***,the nucleation and growth behavior ofδphase in cold-rolled Inconel 718 alloy during aging were *** results show that the precipitation rate and volume fraction ofδphase increase with increasing the cold rolling reduction from 10%to 50%.The volume fraction ofδphase reaches equilibrium after 5 h,remaining at 5.98%,6.52%,and 6.79%under different rolling reductions(10%,30%,and 50%),*** nucleation ofδphase mainly occurs on different sites(grain boundaries,new twin boundaries and old twin boundaries)under 10%rolling reduction,whileδphase mainly nucleates on the new grain boundaries of static recrystallization due to 50%rolling *** the growth ofδphase undergoes a process of alternate orientation growth from spherical(nucleation)→short rod(longitudinal orientation growth)→short rod(radial orientation growth)→dynamic *** 10%rolling reduction,δphase tends to grow into the matrix,while under 50%rolling reduction,the orientation grows faster and is easily affected by the grain boundary curvature.

关键词： Inconel 718 alloy Cold rolling δphase Orientation growth Morphology evolution

Global convergence in terrestrial gross primary production response to atmospheric vapor pressure deficit

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Science China(Life Sciences) 2024年第9期67卷 2016-2025页

作者： Chao Huang Jingfeng Huang Jingfeng Xiao xing Li Hong SHe Yu liang Fusheng Chen Hanqin Tian Key Laboratory of National Forestry and Grassland Administration on Forest Ecosystem Protection and Restoration of Poyang Lake Watershed College of ForestryJiangxi Agricultural UniversityNanchang 330045China CAS Key Laboratory of Forest Ecology and Management Institute of Applied EcologyChinese Academy of SciencesShenyang 110016China Institute of Applied Remote Sensing&Information Technology College of Environmental and Resource SciencesZhejiang UniversityHangzhou 310058China Key Laboratory of Agricultural Remote Sensing and Information Systems Zhejiang ProvinceZhejiang UniversityHangzhou 310058China Earth Systems Research Center Institute for the Study of EarthOceansand SpaceUniversity of New HampshireDurham NH 03824USA Research Institute of Agriculture and Life Sciences Seoul National UniversitySeoul 08826South Korea School of Natural Resources University of Missouri203 ABNR BuildingColumbiaMO 65211USA Schiller Institute for Integrated Science and Society Department of Earth and Environmental SciencesBoston CollegeChestnut HillMA 02467USA

Atmospheric vapor pressure deficit(VPD)increases with climate warming and may limit plant ***,gross primary production(GPP)responses to VPD remain a mystery,offering a significant source of uncertainty in the estimation of global terrestrial ecosystems carbon *** this study,in-situ measurements,satellite-derived data,and Earth System Models(ESMs)simulations were analysed to show that the GPP of most ecosystems has a similar threshold in response to VPD:first increasing and then *** VPD exceeds these thresholds,atmospheric drought stress reduces soil moisture and stomatal conductance,thereby decreasing the productivity of terrestrial *** ESMs underscore CO_(2) fertilization effects but predict significant GPP decline in low-latitude ecosystems when VPD exceeds the *** results emphasize the impacts of climate warming on VPD and propose limitations to future ecosystems productivity caused by increased atmospheric water *** VPD,soil moisture,and canopy conductance interactions into ESMs enhances the prediction of terrestrial ecosystem responses to climate change.

关键词： atmospheric water demands soil moisture stomatal conductance photosynthesis climate warming