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Rosiglitazone prevents murine hepatic fibrosis induced by Schistosoma japonicum

World Journal of Gastroenterology 2008年第18期14卷 2905-2911页

作者： Hui Chen yong-wen he wen-Qi Liu Jing-Hui Zhang Department of Infectious Disease Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 Hubei Province China Department of Parasitology Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 Hubei Province China Department of Surgical Laboratory Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 Hubei Province China

AIM: To evaluate the effect of rosiglitazone in a murine model of liver fibrosis induced by Schistosoma japonicum infection. METHODS: A total of 50 mice were randomly and averagely divided into groups A, B, C, D and E. The mice in group A served as normal controls, while those in the other four groups were infected with Schistosoma japonicum to induce the model of liver fibrosis. Besides, the mice in groups C, D and E were treated with praziquantel, rosiglitazone and praziquantel plus rosiglitazone, respectively. NF-κB binding activity and expression of PPARγ-mRNA were determined by Western blot assay and real-time quantitative PCR. Radioimmunonassay technique was used to detect the serum content changes of TNF-α and IL-6. Histological specimens were stained with he. Expression of TGF-β1, a-smooth muscle actin and type ?Ⅰ?and type Ⅲ collagen was detected by immunohistochemistry and multimedia color pathographic analysis system. RESULTS: Inflammation and fibrosis in the rosiglitazone plus praziquantel treatment group (group E) were lightest among the mice infected with Schistosoma (P her explore the mechanism of rosiglitazone action, we found that rosiglitazone can significantly increase the expression of PPARγ [E: -18.212 ± (-3.909) vs B: -27.315 ± (-6.348) and C: -25.647 ± (-5.694), P he NF-κB binding activity (E: 88.89 ± 19.34 vs B: 141.11 ± 15.37, C: 112.89 ± 20.17 and D: 108.89 ± 20.47, P he serum level of TNF-α (E: 1.613 ± 0.420 ng/mL vs B: 2.892 ± 0.587 ng/mL, C: 2.346 ± 0.371 ng/mL and D: 2.160 ± 0.395 ng/mL, P he hepatic expression of TGF-β1, α-SMA type Ⅰand type Ⅲ collagen in mice with liver fibrosis. CONCLUSION: The activation of PPARγ by its ligand can retard liver fibrosis and suggest the use of rosiglitazone for the treatment

关键词： Peroxisome proliferators-activated receptor γ Rosiglitazone Liver fibrosis Schistosomiasis hepati cstellate cell

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Inhibition of hepatitis B virus replication by pokeweed antiviral protein in vitro

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World Journal of Gastroenterology 2008年第10期14卷 1592-1597页

作者： yong-wen he Chun-Xia Guo Yan-Feng Pan Cheng Peng Zhi-Hong weng Department of Infectious Disease Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 Hubei Province China Department of Infectious Disease the First Affiliated Hospital of Zhengzhou University Zhengzhou 450052 Henan Province China

AIM: To explore the inhibitory effects of pokeweed antiviral protein seed (PAP-S) and PAP encoded by a eukaryotic expression plasmid on hepatitis B virus (HBV) replication in vitro. METHODS: hepG2 2.2.15 cells in cultured medium were treated with different concentrations of PAP-S. HBsAg, HBeAg and HBV DNA in supernatants were determined by ELISA and fluorescent quantitative PCR respectively. MTT method was used to assay for cytotoxicity. hepG2 were cotransfected with various amounts of PAP encoded by a eukaryotic expression plasmid and replication competent wild-type HBV 1.3 fold over- length plasmid. On d 3 after transfection, HBsAg and HBeAg were determined by using ELISA. Levels of HBV core-associated DNA and RNA were detected by using Southern and Northern blot, respectively. RESULTS: The inhibitory effects of PAP-S on HBsAg, HBeAg and HBV DNA were gradually enhanced with the increase of PAP concentration. When the concentration of PAP-S was 10 μg/mL, the inhibition rates of HBsAg, HBeAg and HBV DNA were 20.9%, 30.2% and 50%, respectively. After transfection of 1.0 μg and 2.0 μg plasmid pXF3H-PAP, the levels of HBV nucleocapside- associated DNA were reduced by 38.0% and 74.0% respectively, the levels of HBsAg in the media by 76.8% and 99.7% respectively, and the levels of HBeAg by 72.7% and 99.3% respectively as compared with controls. Transfection with 2 μg plasmid pXF3H-PAP reduced the levels of HBV nucleocapside-associated RNA by 69.0%.CONCLUSION: Both PAP-S and PAP encoded by a eukaryotic expression plasmid could effectively inhibit HBV replication and antigen expression in vitro, and the inhibitory effects were dose-dependent.

关键词： Pokeweed antiviral protein hepatitis B virus Antiviral agent

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Induction of APOBEC3G in human hepatoma cells by interferon-α stimulation

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Journal of Microbiology and Immunology 2006年第3期4卷 222-230页

作者： YAN CHANG LEI yong wen he CHUN XIA GUO Department of Infectious Disease Union Hospital Ton~i Medical College Huazhong University of Scienceand Technology Wuhan P. R. China.

To clarify the role of APOBEC3G （A3G） in cellular defense against hepatitis B virus （HBV）, the expression of A3G in normal human liver and the regulation of the A3G expression in hepatoma cell line （HuH-7） were investigated. Expression level of APOBEC3s mRNA in human liver was determined by RT-PCR. HuH-7 and hepG2 cells were treated with various concentrations of IFN-α（0 U/ml, 100 U/ml, 500 U/ml, 1000 U/ml）for 12 h. The mRNA levels were measured by a quantitative RT-PCR, the results were normalized relative to the specimens without IFN-α stimulation. Total protein of HuH-7 cells treated with various concentrations of IFN-α for 48 h was subjected to Western blot analysis. For reporter gene assay, HuH-7 cells were transfected with the reporter plasmids containing IRF- E sites and its mutants with different lengths. Then the cells were treated with or without 1200 U/ml IFN-α for additional 12 h （ 1000 U/ml） after 24 h of transfection, and the cell lysate was prepared and assayed for lueiferase activity. It was found that normal human liver expressed the rnR_NA of A3G. A3G mRNA expression in HuH-7 and hepG2 cells were up-regulated by IFN-α stimulation in a dose-depen- dent manner. Western blot analysis indicated that A3G protein expression was also enhanced by IFN-α stimulation. Sequence analysis showed the existence of putative sites of IFN regulatory factor element （IRF-E） in 5＇ region of A3G gene upstream the initiation eodon. IFN-α stimulation results in 6- to 8- fold increase in lueiferase activity in cells transfeeted with the plasmid containing IRF-E sites of the 5＇ upstream sequences, whereas luciferase activity did not change in cells transfected with the plasmid containing mutant IRF-E sites or without IRF-E sites. As a conclusion, A3G are expressed in normal human liver. A3G expression was up-regulated by IFN-α stimulation in hepatoma cells and could be involved in host defense mechanisms against HBV. IRF-E site in 5＇ region of APOBEC3G gene upstream the init

关键词： APOBEC3G IFN-α hepatoma cell IRF-E

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中国西部含油气盆地石油地质条件的主要认识和勘探方向

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石油学报 2004年第6期25卷 1-7页

作者：陈永武何文渊中国石油天然气集团公司咨询中心北京100724

我国西部的盆地基本上都属于小型克拉通盆地 ,其石油地质特征可以概括为 :①盆地主要为叠合盆地 ,具有多期构造发育史 ,造成油气资源分散 ;②多套烃源岩复合叠置 ,多阶段成烃演化与油气多期成藏 ;③两套构造层和多套沉积沉序形成多种储... 详细信息

我国西部的盆地基本上都属于小型克拉通盆地 ,其石油地质特征可以概括为 :①盆地主要为叠合盆地 ,具有多期构造发育史 ,造成油气资源分散 ;②多套烃源岩复合叠置 ,多阶段成烃演化与油气多期成藏 ;③两套构造层和多套沉积沉序形成多种储集类型 ;④多套沉积层序和后期构造运动控制的油气多期成藏决定了勘探的多目的层和勘探工作的复杂性 ,晚期成藏现象尤其值得重视 ;⑤油气藏类型多。各层系圈闭和油气藏类型丰富 ,前陆盆地冲断带主要是构造型 ,克拉通区油气藏类型主要是构造、岩性、地层和复合型油气藏。根据这些石油地质特征 ,西部地区的重点勘探领域主要分布在继承性的古隆起区、前陆盆地和正向构造上的岩性地层带。参照西部地区年均新增探明油气储量的趋势 ,未来 10年内年新增探明油气可采储量可保持在 0 5～ 0 7亿t和 15 0 0～2 0 0 0亿m3 。

关键词：构造运动油气成藏勘探储量中国西部

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The detection, extraction and parameter estimation of extreme-mass-ratio inspirals with deep learning

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 86-96页

作者： Qianyun Yun wen-Biao Han Yi-Yang Guo he Wang Minghui Du Hangzhou Institute for Advanced Study University of Chinese Academy of Sciences Shanghai Astronomical Observatory Chinese Academy of Sciences School of Physics and Astronomy Shanghai Jiao Tong University School of Astronomy and Space Science University of Chinese Academy of Sciences Taiji Laboratory for Gravitational Wave Universe (Beijing/Hangzhou) University of Chinese Academy of Sciences Lanzhou Center for Theoretical Physics Key Laboratory of Theoretical Physics of Gansu Province Key Laboratory of Quantum Theory and Applications of MoE Lanzhou University Institute of Theoretical Physics & Research Center of Gravitation Lanzhou University International Centre for Theoretical Physics Asia-Pacific (ICTP-AP) University of Chinese Academy of Sciences (UCAS) Center for Gravitational Wave Experiment National Microgravity Laboratory Institute of Mechanics Chinese Academy of Sciences

One of the primary goals of space-borne gravitational wave detectors is to detect and analyze extreme-mass-ratio inspirals(EMRIs). This task is particularly challenging because EMRI signals are complex, lengthy, and faint. In this work, we introduce a2-layer convolutional neural network(CNN) approach to detect EMRI signals for space-borne detectors, achieving a true positive rate(TPR) of 96.9% at a 1% false positive rate(FPR) for signal-to-noise ratio(SNR) from 50 to 100. Especially, the key intrinsic parameters of EMRIs such as the mass, spin of the supermassive black hole(SMBH) and the initial eccentricity of the orbit can also be inferred directly by employing a neural network. The mass and spin of the SMBH can be determined at 99% and 92% respectively. This will greatly reduce the parameter spaces and computing cost for the following Bayesian parameter *** model also has a low dependency on the accuracy of the waveform model. This study underscores the potential of deep learning methods in EMRI data analysis, enabling the rapid detection of EMRI signals and efficient parameter estimation.

关键词： gravitational wave EMRIs deep-learing

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罗格列酮对日本血吸虫病肝纤维化小鼠肝组织核因子-κB和过氧化物酶体增殖物激活受体γ表达的影响

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世界华人消化杂志 2007年第7期15卷 741-745页

作者：谌辉张景辉刘文琪贺永文华中科技大学同济医学院附属协和医院传染科湖北省武汉市430030 华中科技大学同济医学院附属协和医院外科实验室湖北省武汉市430030 华中科技大学同济医学院寄生虫学教研室湖北省武汉市430022

目的:观察日本血吸虫病肝纤维化小鼠肝脏肝组织核因子-κB(NF-κB)的活性和过氧化物酶体增殖物激活受体γ(PPARγ)的表达,及PPARγ配体罗格列酮对其表达的影响.方法:50只昆明小鼠,随机分为正常对照组、感染对照组、吡喹酮治疗组、罗格... 详细信息

目的:观察日本血吸虫病肝纤维化小鼠肝脏肝组织核因子-κB(NF-κB)的活性和过氧化物酶体增殖物激活受体γ(PPARγ)的表达,及PPARγ配体罗格列酮对其表达的影响.方法:50只昆明小鼠,随机分为正常对照组、感染对照组、吡喹酮治疗组、罗格列酮治疗组及罗格列酮加吡喹酮治疗组.除正常对照组外,其余各组均建立血吸虫病肝纤维化小鼠模型.用he染色观察肝组织光镜下的病理改变.用Western blot方法,实时荧光定量PCR反应观察小鼠肝组织NF-κB的活性变化与PPARγmRNA的表达.结果:罗格列酮加吡喹酮治疗组小鼠肝脏的炎性反应和纤维化病理改变较其他模型组轻(P<0.05).感染对照组NF-κB活性(141.11±15.37)最强,明显高于其余各组(正常对照组:78.89±18.12;吡喹酮组:112.89±20.17;罗格列酮组:108.89±20.47;罗格列酮加吡喹酮组:88.89±19.34)(P<0.05).感染对照组[-27.315±(-6.348)]及吡喹酮治疗组[-25.647±(-5.694)]PPARγmRNA表达较正常对照组[-16.557±(-3.022)]及罗格列酮治疗组[-18.217±(-4.498)]、罗格列酮加吡喹酮治疗组[-18.212±(-3.909)]显著减弱(P<0.05).结论:PPARγ及NF-κB在血吸虫病肝纤维化形成中起一定作用.PPARγ配体罗格列酮有明显的抗日本血吸虫病肝纤维化效应,其抗纤维化机制与PPARγ配体激活PPARγ表达的同时抑制NF-κB的活性有关.

关键词：罗格列酮日本血吸虫病核因子-κB 过氧化物酶体增殖物激活受体γ

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罗格列酮对小鼠日本血吸虫病肝纤维化基质金属蛋白酶-2的影响

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世界华人消化杂志 2007年第18期15卷 2046-2049页

目的:研究PPARγ配体罗格列酮治疗小鼠血吸虫病肝纤维化,血清MMP-2与TIMP-2的变化及肝组织MMP-2与TIMP-2的基因表达方法:昆明小鼠50只,随机分为正常对照组A、感染对照组B、吡喹酮治疗组C、罗格列酮治疗组D及罗格列酮加吡喹酮治疗组E.除... 详细信息

目的:研究PPARγ配体罗格列酮治疗小鼠血吸虫病肝纤维化,血清MMP-2与TIMP-2的变化及肝组织MMP-2与TIMP-2的基因表达方法:昆明小鼠50只,随机分为正常对照组A、感染对照组B、吡喹酮治疗组C、罗格列酮治疗组D及罗格列酮加吡喹酮治疗组E.除正常对照组外,其余各组均建立血吸虫病肝纤维化小鼠模型.用ELISA法检测血清MMP-2及TIMP-2的含量,实时荧光定量PCR反应观察小鼠肝组织MMP-2 mRNA及TIMP-2 mRNA的表达.结果:D,E组血清MMP-2的含量(306.0±62.3μg/L,312.0±54.3μg/L)及肝组织MMP-2 mRNA的表达[-19.1±(-6.0),-20.4±(-6.2)]高于A组[221.3±39.2μg/L,-26.3±(-5.3):P0.05).结论:MMP-2在血吸虫病肝纤维化形成中起促进作用,PPARγ配体罗格列酮的抗肝纤维化机制与其下调MMP-2的表达有一定关系.

关键词：过氧化物酶体增殖物激活受体γ 肝纤维化 MMP-2 TIMP-2

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Photothermal probiotics delivery of photodynamic agents for enhanced immunogenic cell death within hypoxia center of solid tumors

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Nano Research 2025年第1期18卷 642-652页

作者： wen Luo Shiyu Du Yateng Jiang Bangshun he Jun Tang Jingjing Yang Yujun Song Jiangsu Key Laboratory of Artificial Functional Materials State Key Laboratory of Analytical Chemistry for Life ScienceNational Laboratory of Solid State MicrostructuresCollege of Engineering and Applied SciencesNanjing UniversityNanjing 210023China Department of Laboratory Medicine Nanjing First HospitalNanjing Medical UniversityNanjing 210006China Department of Anesthesiology Jinling HospitalMedical School of Nanjing UniversityNanjing 210000China Department of Biochemistry and Molecular Biology School of Medicine&Holistic Integrative MedicineJiangsu Collaborative Innovation Center of Chinese Medicinal Resources IndustrializationNanjing University of Chinese MedicineNanjing 210023China

Resulting from harsh hypoxic environmental conditions,central cells in the core of solid tumors are usually more aggressive and malignant with a less stable ***,therapeutic agents with improved penetration for the activation of immunity in tumor centers exhibit promising potential in ***,polydopamine-coated Escherichia coli Nissle 1917(EcN)bearing chlorin e6(Ce6)-loaded and polyethyleneimine(PEI)-coated hollow manganese dioxide(shorted as EP-ChP)are applied for enhanced immunotherapy in deep *** accumulation in tumor center through hypoxia targeting,manganese dioxide is degradated under the tumor microenvironment with released Ce6 and thus generates reactive oxygen species(ROS)upon 660 nm laser irradiation,which can further lower thermalresistance of cancer cells via HSP90α*** to that,heating induced by polydopamine upon 808 nm laser irradiation can achieve effective tumor *** upon dual laser induces enhanced immunogenic cell death,while bacterial infections in tumor tissues also trigger innate *** bacteria-based approach provides enhanced antitumor immune responses in deep tumors with great potential in the reshaping of immunosuppression tumor microenvironment.

关键词： bacteria phototherapies immunotherapy hypoxia targeting tumor penetration

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Recent progress in constructing fluorinated solid-electrolyte interphases for stable lithium metal anodes

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 270-291页

作者： Di Zhang Pengfei Lv Wei Qin Xin he Yuanhua he Civil Aircraft Fire Science and Safety Engineering Key Laboratory of Sichuan Province College of Civil Aviation Safety EngineeringCivil Aviation Flight University of ChinaGuanghan 618307China School of Chemical Engineering Sichuan UniversityChengdu 610065China

Lithium metal batteries(LMBs)are emerging as a promising energy storage solution owing to their high energy density and specific ***,the non-uniform plating of lithium and the potential rupture of the solid-electrolyte interphase(SEI)during extended cycling use may result in dendrite growth,which can penetrate the separator and pose significant short-circuit *** a stable SEI is essential for the long-term operation of the ***-rich SEI has garnered significant attention for its ability to effectively passivate electrodes,regulate lithium deposition,and inhibit electrolyte *** the structural components and preparation methods of existing fluorinated SEI is crucial for optimizing lithium metal anode *** paper reviews the research on optimizing LiF passivation interfaces to protect lithium metal *** focuses on four types of compositions in fluorinated SEI that work synergistically to enhance SEI *** instance,combining compounds with LiF can further enhance the mechanical strength and ionic conductivity of the *** metals with LiF significantly improves electrochemical performance at the SEI/anode interface,with a necessary focus on reducing electron tunneling ***,incorporating polymers with LiF offers balanced improvements in interfacial toughness and ionic conductivity,though maintaining structural stability over long cycles remains a critical area for future *** alloys combined with LiF increase surface energy and lithium affinity,challenges such as dendrite growth and volume expansion *** summary,this paper emphasizes the crucial role of interfacial structures in LMBs and offers comprehensive guidance for future design and development efforts in battery technology.

关键词： LiF lithium metal anodes solid-electrolyte interphase interface cycling stability

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天晴甘平减轻急性肝衰竭小鼠肝细胞损伤的机制

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世界华人消化杂志 2009年第10期17卷 1014-1017页

作者：刘凤超贺永文喻佛定华中科技大学同济医学院附属协和医院感染科湖北省武汉市430022

目的:探讨天晴甘平减轻急性肝衰竭肝细胞损伤的机制.方法:54只健康雌性昆明小白鼠随机分为实验组、对照组、正常对照组3组,每组18只.腹腔注射D-氨基半乳糖(D-galn)和脂多糖(LPS)制作急性肝衰竭模型.注射前3h,实验组给予天晴甘平灌胃,分... 详细信息

目的:探讨天晴甘平减轻急性肝衰竭肝细胞损伤的机制.方法:54只健康雌性昆明小白鼠随机分为实验组、对照组、正常对照组3组,每组18只.腹腔注射D-氨基半乳糖(D-galn)和脂多糖(LPS)制作急性肝衰竭模型.注射前3h,实验组给予天晴甘平灌胃,分别于注射后不同时间点用彗星实验检测肝细胞核DNA的损伤.结果:实验组和对照组的Olive尾矩值在0.5h即开始增大,且随时间延伸逐渐增加,与正常对照组比较,差异均具显著意义(0.5h:3.95±1.42,4.79±1.62vs0.95±0.56,均P<0.05);实验组与对照组比较,实验组Olive尾矩值明显变小,差异亦具显著意义(0.5-8h:10.81±2.85vs19.36±3.95,P<0.05).结论:急性肝衰竭小鼠肝细胞损伤的发生机制之一为肝细胞核DNA的损伤,天晴甘平具有减轻急性肝衰竭小鼠肝细胞核DNA损伤的作用.

关键词：急性肝衰竭天晴甘平彗星实验 Olive尾矩值

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