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A feasible,economical,and accurate analytical method for simultaneous determination of six alkaloid markers in Aconiti Lateralis Radix Praeparata from different manufacturing sources and processing ways

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Chinese Journal of Natural Medicines 2017年第4期15卷 301-309页

作者： ZHANG Yi-Bei DA Juan ZHANG Jing-Xian LI Shang-Rong CHEN Xin LONG Hua-Li wang qiu-Rong CAI lu-Ying yao Shuai HOU Jin-jun WU Wan-Ying GUO De-An Shanghai Research Center for Traditional Chinese Medicine and National Engineering Laboratory for TCM Standardization Technology Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China

Aconiti Lateralis Radix Praeparata(Fuzi) is a commonly used traditional Chinese medicine in clinic for its potency in restoring yang and rescuing from collapse. Aconiti alkaloids, mainly including monoester-diterpenoidaconitines(MDAs) and diester-diterpenoidaconitines(DDAs), are considered to act as both bioactive and toxic constituents. In the present study, a feasible, economical, and accurate HPLC method for simultaneous determination of six alkaloid markers using the Single Standard for Determination of Multi-Components(SSDMC) method was developed and fully validated. Benzoylmesaconine was used as the unique reference standard. This method was proven as accurate(recovery varying between 97.5%-101.8%, RSD 0.999 9) over the concentration ranges, and subsequently applied to quantitative evaluation of 62 batches of samples, among which 45 batches were from good manufacturing practice(GMP) facilities and 17 batches from the drug market. The contents were then analyzed by principal component analysis(PCA) and homogeneity test. The present study provided valuable information for improving the quality standard of Aconiti Lateralis Radix Praeparata. The developed method also has the potential in analysis of other Aconitum species, such as Aconitum carmichaelii(prepared parent root) and Aconitum kusnezoffii(prepared root).

关键词： Aconiti Lateralis Radix Praeparata Single Standard for Determination of Multi-Components (SSDMC) Monoester-literpenoidaconitines (MDAs) Diester-diterpenoidaconitines (DDAs)

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Plasmacytoid Dendritic Cell Function and Cytokine Network Profiles in Patients with Acute or Chronic Hepatitis B Virus Infection

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Chinese Medical Journal 2018年第1期131卷 43-49页

作者： Ming-Hui Li lu Zhang Dan Zhang wei-Hua Cao Tian-Lin Qi Hong-Xiao Hao Xing-Yue wang chong-Ping Ran Xiao-Jing Qu Shun-Ai Liu yao lu Ge Shen Shu-Ling Wu Min Chang Ru-Yu Liu Lei-Ping Hu Wen-Hao Hua Gang Wan jun Cheng yao Xie Liver Diseases Center Beijing Ditan Hospital Capital Medical University Beijing 100015 China Institute of Infectious Diseases Beijing DitanHospitai Capital Medical University Beijing 100015 China Clinical Test Center Beijing Ditan Hospital Capital Medical University Beijing 100015 China

Background： Plasmacytoid dendritic cells （pDCs） and cytokines play an important role in occurrence and recovery of hepatitis B virus （HBV） infection. The aim of this study was to explore the frequency and function ofpDC and serum cytokine network profiles in patients with acute or chronic HBV infection. Methods： The healthy individuals （HI group）, hepatitis B envelope antigen （HBeAg）-positive chronic HBV patients in immune tolerance （IT） phase （IT group）, HBeAg-positive chronic HBV patients （CHB group）, and acute HBV patients （AHB group） were enrolled in this study. The frequency of cluster of differentiation antigen 86 （CD86）＋ pDC and the counts of CD86 molecular expressed on surface ofpDC were tested by flow cytometer. The quantitative determinations ofcytokines, including Fins-like tyrosine kinase 3 ligand （FIt-3L）, interferon （1FN）-α2, IFN-γ, interleukin （IL）-17A, IL-6, IL-10, transforming growth factor （TGF）-β1 and TGF-β2, were performed using luminex multiplex technology. Results： In this study, there were 13 patients in HI group, 30 in IT group, 50 in CHB group, and 32 in AHB group. Compared with HI group, HBV infected group （including all patients in IT, CHB and AHB groups） had significantly higher counts of CD86 molecular expressed on the surface ofpDC （4596.5 ± 896.5 vs. 7097.7 ± 3124.6; P 〈 0.001）. The counts of CD86 molecular expressed on the surface of pDC in CriB group （7739.2 ±4125.4） was significantly higher than that of IT group （6393.4 ± 1653.6, P=0.043）. Compared with IT group, the profile of cytokines of FIt-3L, IFN-γ, and IL-17A was decreased, IFN-α2 was significantly increased （P =0.012） in CH B group. The contents of IL-10, TGF-{31, and TGF-132 in AHB group were significantly increased compared with IT and CHB groups （all P 〈 0.05）. Conclusions： This study demonstrated that the function of pDC was unaffected in HBV infection. The enhanced function of pDC and IFN-α2 might involve triggering the immune response from IT to hepatitis active phase

关键词： Acute Hepatitis B Virus Infection Chronic Hepatitis B Virus Infection Interferon-α2 Interleukin-10 PlasmacytoidDendritic Cells

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Autism Candidate Gene DIP2A Regulates Spine Morphogenesis via Acetylation of Cortactin

Autism Candidate Gene DIP2A Regulates Spine Morphogenesis vi...

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中国生理学会张锡钧基金第十五届全国青年优秀生理学学术论文交流及评奖会议;中国生理学会第十三届全国青年生理学工作者学术会议

作者： jun Ma lu-Qing Zhang Zi-Xuan He Xiao-Xiao He Ya-jun wang You-Li Jian Xin wang Bin-Bin Zhang Ce Su jun lu Bai-Qu Huang Yu Zhang Gui-Yun wang wei-Xiang Guo De-Lai qiu Lin Mei Wen-Cheng Xiong yao-Wu Zheng Xiao-Juan Zhu Key Laboratory of Molecular Epigenetics Institute of Genetics and CytologyNortheast Normal University State Key Laboratory for Molecular and Developmental Biology Institute of Genetics and Developmental BiologyChinese Academy of Sciences School of Life Sciences Yunnan University Key Laboratory of Cellular Function and Pharmacology of Jilin Province Yanbian University Department of Neurosciences Case Western Reserve University

Dendritic spine development is crucial for the establishment of excitatory synaptic connectivity and functional neural *** in spine morphology and density have been associated with multiple neurological *** in the disconnected-interacting protein homologue 2 A(DIP2A) have been associated with developmental delay,substantial susceptibility to autism spectrum disorder (ASD) and dyslexia.

关键词： cortactin Autism Candidate Gene DIP2A Regulates Spine Morphogenesis via Acetylation of Cortactin

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基于个体化机器学习的原发性免疫性血小板减少症危重出血预测模型:一项全国前瞻性队列研究

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科学通报:英文版 2023年第18期68卷 2106-2114,M0004页

作者： Zhuo-Yu An Ye-jun Wu Yu Hou Heng Mei wei-Xia Nong Wen-Qian Li Hu Zhou Ru Feng Jian-Ping Shen jun Peng Hai Zhou Yi Liu yong-Ping Song Lin-Hua Yang Mei-Yun Fang Jian-yong Li Yun-Feng Cheng Peng Liu Ya-Jing Xu Zhao wang Yi luo Zhen Cai Hui Liu Jing-Wen wang Juan Li Xi Zhang Zi-Min Sun Xiao-Yu Zhu Xin wang Rong Fu Liang Huang Shao-Yuan wang Tong-Hua Yang Li-Ping Su Liang-Ming Ma Xie-Qun Chen Dai-Hong Liu Hong-Xia yao Jia Feng Hong-Yu Zhang Ming Jiang Ze-Ping Zhou Wen-Sheng wang Xu-Liang Shen Yangjin Baima Yue-Ying Li Qian-Fei wang qiu-Sha Huang Hai-Xia Fu Xiao-lu Zhu Yun He Qian Jiang Hao Jiang Jin lu Xiang-Yu Zhao Ying-jun Chang Tao Wu yao-Zhu Pan Lin qiu Da Gao A-Rong Jin wei Li Su-jun Gao Lei Zhang Ming Hou Xiao-jun Huang Xiao-Hui Zhang on behalf of the National Cooperative ITP Working Group Peking University People’s Hospital Peking University Institute of HematologyBeijing 100044China Collaborative Innovation Center of Hematology Peking UniversityBeijing 100044China Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation Beijing 100044China National Clinical Research Center for Hematologic Disease Beijing 100044China Department of Hematology Qilu HospitalCheeloo College of MedicineShandong UniversityJinan 250014China Institute of Hematology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430074China Department of Hematology First Affiliated HospitalSchool of MedicineShihezi UniversityShihezi 832002China Department of Hematology Qinghai Provincial People’s HospitalXining 810007China Henan Cancer Hospital Affiliated Cancer Hospital of Zhengzhou UniversityHenan Institute of HematologyZhengzhou 450008China Department of Hematology Beijing HospitalBeijing 100044China Department of Hematology Zhejiang Provincial Hospital of Traditional Chinese MedicineHangzhou 310006China Department of Hematology Senior Department of Hematologythe Fifth Medical Center of PLA General HospitalBeijing 100044China Department of Hematology the Second Hospital of Shanxi Medical UniversityTaiyuan 030001China Department of Hematology Affiliated Zhongshan Hospital of Dalian UniversityDalian 116001China Department of Hematology The First Affiliated Hospital with Nanjing Medical University(Jiangsu Province Hospital)Nanjing 210029China Department of Hematology Zhongshan HospitalFudan UniversityShanghai 200032China Department of Hematology Xiangya HospitalCentral South UniversityChangsha 410008China Department of Hematology Beijing Friendship HospitalCapital Medical UniversityBeijing 100044China Institute of Hematology the First Affiliated HospitalZhejiang UniversityHangzhou 310058China Department of Hematology Beijing Tongren HospitalBeijing 100005China Department of Hematology The First Affiliated HospitalSun Yat-sen UniversityGuang

原发性免疫性血小板减少症(ITP)中少见但至关重要的危重出血事件,给患者的预后、生活质量和治疗决策带来严重影响。尽管有一些研究探讨了ITP中与危重出血相关的风险因素,但目前尚缺乏大样本数据、大规模多中心研究结果以及针对ITP患者... 详细信息

原发性免疫性血小板减少症(ITP)中少见但至关重要的危重出血事件,给患者的预后、生活质量和治疗决策带来严重影响。尽管有一些研究探讨了ITP中与危重出血相关的风险因素,但目前尚缺乏大样本数据、大规模多中心研究结果以及针对ITP患者致命出血事件的预测模型。本研究首次采用国际血栓与止血学会新提出的ITP致命出血标准,利用大样本数据开发了首个基于机器学习的在线应用,用于预测ITP患者的致命出血.研究中,我们使用中国各地大型多中心数据进行开发,并对全国39家医疗中心进行为期一年的外部测试,得到了较好的训练、验证和测试数据集预测能力该基于新算法的便捷网络工具能够快速识别ITP患者的出血风险,辅助临床决策,有望未来降低不良事件的发生。

关键词： Critical bleeding Severe bleeding Immune thrombocytopenia Machine learning Prediction model

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Autism candidate gene DIP2A regulates spine morphogenesis via acetylation of cortactin

Autism candidate gene DIP2A regulates spine morphogenesis vi...

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2019中国生理学会学术年会暨张锡钧基金第十五届全国青年优秀生理学学术论文交流会及第十三届全国青年生理学工作者学术会议

作者：马俊 lu-Qing Zhang Zi-Xuan He Xiao-Xiao He Ya-jun wang You-Li Jian Xin wang Bin-Bin Zhang Ce Su jun lu Bai-Qu Huang Yu Zhang Gui-Yun wang wei-Xiang Guo De-Lai qiu Lin Mei Wen-Cheng Xiong yao-Wu Zheng Xiao-Juan Zhu Key Laboratory of Molecular Epigenetics Institute of Genetics and CytologyNortheast Normal University State Key Laboratory for Molecular and Developmental Biology Institute of Genetics and Developmental BiologyChinese Academy of Sciences School of Life Sciences Yunnan University Key Laboratory of Cellular Function and Pharmacology of Jilin Province Yanbian University Department of Neurosciences Case Western Reserve University

Dendritic spine development is crucial for the establishment of excitatory synaptic connectivity and functional neural *** in spine morphology and density have been associated with multiple neurological *** in the disconnected-interacting protein homologue 2 A (DIP2A) have been associated with developmental delay, substantial susceptibility to autism spectrum disorder (ASD) and dyslexia.

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Common susceptibility loci in both systemic sclerosis and localized scleroderma identified using genetic analysis

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Chinese Medical Journal 2020年第19期133卷 2370-2372页

作者： Yun Li Wen-jun wang wei-wei Chen Xue Fan lu Cao Ze-Yu Xing Qi Zhen Qiong-Qiong Xu Chen-Yu Zhu Hui-yao Ge Dian Chen Rui-Xue Zhang Chang Shu wei Du Shi-Rui Chen Xie Yuan Hui Zhang Xia Hu Xu-Ming Mao qiu-Ning Sun Department of Dermatology Beijing Tian Tan HospitalCapital Medical UniversityBeijing 100059China Department of Dermatology No.1 HospitalAnhui Medical UniversityHefeiAnhui 230022China Department of Dermatology Chinese Academy of Medical Sciences Peking Union Medical CollegePeking Union Medical College HospitalBeijing 100730China Department of Dermatology University of PennsylvaniaPhiladelphiaPA 19104USA.

Scleroderma is an autoimmune fibrosing disorder that can be further subclassified as localized scleroderma(LSc)and systemic sclerosis(SSc).LSc is characterized by sclerotic and pigmented skin lesions,while SSc is a more generalized disorder of the connective tissue involving a number of *** is characterized by the thickening of dermal collagen bundles,fibrosis,and vascular abnormalities in the visceral organs.[1]Despite the differences in their morphologic features and clinical presentation,these two diseases do share some characteristics including endothelial cell dysfunction,T helper 2(Th2)cell dominance during immune activation and excess fibrosis of the skin with similar pathologic findings,leading to the hypothesis that SSc and LSc share an underlying mechanism of ***,multiple lines of evidence suggest that genetic factors may contribute to SSc susceptibility.

关键词： organs susceptibility spite

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用谷氨酸为交联剂制备玉米交联氧化淀粉胶黏剂及性能研究

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包装工程 2023年第21期44卷 134-143页

作者：卢秋晓陈俊颖廖俊杰汪志伟姚凯曾小君江西省化学工业高级技工学校南昌330039 常熟理工学院材料工程学院江苏常熟215500

目的改善淀粉胶黏剂胶合强度低、耐水性差等缺点,通过对玉米淀粉(CS)进行改性,制备玉米交联氧化淀粉胶黏剂(CCOSA)。方法以CS为主要原料,以生物质材料谷氨酸为交联剂,以过氧化氢为氧化剂,采用先交联后氧化的方法制备CCOSA。通过单因素... 详细信息

目的改善淀粉胶黏剂胶合强度低、耐水性差等缺点,通过对玉米淀粉(CS)进行改性,制备玉米交联氧化淀粉胶黏剂(CCOSA)。方法以CS为主要原料,以生物质材料谷氨酸为交联剂,以过氧化氢为氧化剂,采用先交联后氧化的方法制备CCOSA。通过单因素试验确定制备玉米交联淀粉(CCS)和玉米交联氧化淀粉(CCOS)的最优条件,通过FT-IR对制备的CCS和CCOS进行结构表征,并测定制备的CCOSA的基本理化性能、耐水时间和胶合强度。结果得到了制备CCS的优化工艺条件,当CS的质量为20 g、谷氨酸的质量为1.0 g、pH为9、反应温度为55℃、反应时间为1.5 h时,制得的CCS的沉降积(0.55 mL)最小、交联度最大。得到了制备CCOS的优化工艺条件,在CCS的质量为20 g、FeSO4的用量为CCS质量的0.1%、质量分数为30%过氧化氢的用量(体积)为2 mL、反应温度为50℃、pH为3、反应时间为3 h时,制得的CCOS的羧基含量为0.184%,对CCS的氧化效果最好。通过FT-IR分析可知,CCS在1157.22 cm^(-1)处出现了C—N伸缩振动吸收峰,CCOS在1731.35 cm^(-1)处出现了C=O伸缩振动吸收峰,此吸收峰向高波数移动,而且此峰相对于1653.25 cm^(-1)典型的淀粉衍生物C=O吸收峰的强度明显增强。在此条件下制得的CCOSA的胶合强度为6.15 MPa,比玉米交联淀粉胶黏剂(CCSA)、玉米氧化淀粉胶黏剂(COSA)和未改性玉米淀粉胶黏剂(CSA)的胶合强度分别提高了38.83%、107.07%、352.21%,并且CCOSA的耐水性得到明显提高。结论FT-TR分析结果表明,采用先交联后氧化的方法成功制得了CCOS,制得的CCOSA的胶合强度和耐水性得到明显提高。

关键词：玉米淀粉谷氨酸胶黏剂改性胶合强度

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甲醛短期经口暴露致大鼠血清生化指标改变和肝肾胃急性损伤效应研究

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环境与健康杂志 2018年第12期35卷 1040-1043页

作者：顾雯王璐瑶段链王超石莹王翀徐永俊张宏伟中国疾病预防控制中心环境与健康相关产品安全所环境毒理室

目的探讨甲醛短期经口暴露对大鼠血清生化指标和肝、肾、胃组织的影响。方法将80只健康成年SPF级Wistar大鼠随机分为4组,分别为对照(去离子水)组和15、45、135 mg/kg甲醛染毒组,每组20只,雌雄各半。采用经口灌胃方式进行染毒,染毒容量为... 详细信息

目的探讨甲醛短期经口暴露对大鼠血清生化指标和肝、肾、胃组织的影响。方法将80只健康成年SPF级Wistar大鼠随机分为4组,分别为对照(去离子水)组和15、45、135 mg/kg甲醛染毒组,每组20只,雌雄各半。采用经口灌胃方式进行染毒,染毒容量为10 ml/kg,每天1次,连续28 d。于染毒14、28 d,每组选择10只大鼠,检查血清血生化指标[包括丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、血尿素氮(BUN)、总蛋白(TP)、白蛋白(ALB)]水平,观察肝、肾、胃病理学变化情况。结果染毒14 d后,各甲醛染毒组大鼠血清ALT、AST、BUN、TP、ALB的水平与对照组相比,差异均无统计学意义(P>0.05)。染毒28 d后,与对照组相比,45 mg/kg甲醛染毒组雄性大鼠血清ALT的浓度升高,45、135 mg/kg甲醛染毒组雌性大鼠血清中ALT和AST水平降低,差异均有统计学意义(P<0.05);各浓度甲醛染毒组大鼠血清BUN、TP及ALB水平均无明显改变。染毒14 d时,45mg/kg甲醛染毒组雄性大鼠、135 mg/kg甲醛染毒组雄性及雌性大鼠前胃上皮增生和角化过度现象明显,差异有统计学意义(P<0.05)。结论大鼠短期经口摄入甲醛能够引起消化系统损伤。

关键词：甲醛短期暴露健康效应

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^(18)FDG标准化摄取值对非小细胞肺癌三维适形放疗近期疗效的预测作用

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中国医科大学学报 2009年第4期38卷 302-304页

作者：裘敬平党军王天禄姚雷李光中国医科大学附属第一医院放疗科沈阳110001

目的探讨非小细胞肺癌(NSCLC)患者在三维适形放疗前接受18氟脱氧葡萄糖正电子发射断层扫描(18FDG-PET)及计算得出的标准化摄取值(SUV)对放疗疗效的预测作用。方法41例NSCLC患者三维适形放疗前行18FDG-PET/CT扫描并计算SUV,放疗后3个月... 详细信息

目的探讨非小细胞肺癌(NSCLC)患者在三维适形放疗前接受18氟脱氧葡萄糖正电子发射断层扫描(18FDG-PET)及计算得出的标准化摄取值(SUV)对放疗疗效的预测作用。方法41例NSCLC患者三维适形放疗前行18FDG-PET/CT扫描并计算SUV,放疗后3个月评价疾病早期疗效,多因素分析完全缓解、治疗有效的预测因子。结果SUV、状态评分和肿瘤体积影响是完全缓解的预测因素,SUV的OR值为1.717;SUV、状态评分和临床分期影响是治疗有效的预后因素,SUV的OR值为1.123。结论18FDG-PET扫描及计算SUV能够预测NSCLC行三维适形放疗疗效,其敏感性和特异性较高。

关键词：标准化摄取值非小细胞肺癌三维适形放疗疗效

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以间苯二酚-甲醛溶胶和MCM-48原粉制备有序介孔炭分子筛

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无机材料学报 2009年第1期24卷 23-28页

作者：王重庆宋军姚建峰李青张利雄刘晓勤陆现彩南京工业大学化学化工学院材料化学工程国家重点实验室南京210009 南京大学地球科学系南京210093

以含有机模板的介孔纯硅分子筛MCM-48原粉为模板,以间苯二酚（R）-甲醛溶胶为炭源制备了有序介孔炭分子筛.考察了w（R）/w（MCM-48）和w（R）/w（H2O）对合成介孔炭分子筛的影响.产物用N2吸附/脱附、XRD、SEM、TEM和TG等手段进行了表征... 详细信息

以含有机模板的介孔纯硅分子筛MCM-48原粉为模板,以间苯二酚（R）-甲醛溶胶为炭源制备了有序介孔炭分子筛.考察了w（R）/w（MCM-48）和w（R）/w（H2O）对合成介孔炭分子筛的影响.产物用N2吸附/脱附、XRD、SEM、TEM和TG等手段进行了表征.结果表明,w（R）/w（MCM-48）和w（R）/w（H2O）对所得到的介孔炭分子筛的有序性和比表面积有较大的影响.优化的w（R）/w（MCM-48）比为0.50～0.65、w（R）/w（H2O）比为0.15～0.35,此时,能形成比表面积大于1125m^2/g、平均孔径在2.2～2.4nm、具有规则介孔结构的炭分子筛,且这种炭分子筛具有高的热稳定性.

关键词：介孔炭分子筛 MCM-48 间苯二酚甲醛

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