检索结果-南通市图书馆

Loss of Wnt5a and Ror2 protein in hepatocellular carcinoma associated with poor prognosis

World Journal of Gastroenterology 2012年第12期18卷 1328-1338页

作者： Ming geng yong-Cheng Cao Ying-Jian Chen Hui Jiang Li-Quan Bi Xiao-Hong Liu Department of Pathology General Hospital of JinanMilitary Command Jinan 250031 Shandong Province China Department of Laboratory Medicine GeneralHospital of Jinan Military Command Jinan 250031 ShandongProvince China

AIM： To investigate the expression and clinical significance of Wnt member 5a （Wnt5a） and receptor tyrosine kinase-like orphan receptor 2 （Ror2） in hepatocellular carcinoma （HCC）.METHODS： In HCC tissues obtained from 85 patients, the protein expressions of Wnt5a, Rot2, 13-catenin, and Ki-67 via immunohistochemical staining using the Envision Plus System. The antibody binding was visualized with 3, 3＇-diaminobenzidine tetrahydrochloride （DAB） before brief counterstaining with Mayer＇s hematoxylin. The degree of immunohistochemical staining was recorded using a semiquantitative and subjective grading system. The mRNA expression of Rot2 was examined by real time reverse transcription polymerase chain reaction, including nineteen of the 85 HCC and three normal liver tissues. The ratios of Ror2 to the housekeeping gene GAPDH represented the normalized relative levels of Ror2 expression. To determine the prognostic factor, the outcome of the 82 patients was determined by reviewing their medical charts. The overall and diseasefree survival rates were estimated using the Kaplan-Meier method and compared with the logrank test. The prognostic analysis was carried out with univariate and multivariate Cox regressions models.RESULTS： Compared to nontumorous （hepatitis or cirrhotic） tissues, Rot2 mRNA expression was clearly decreased in HCC. Rot2 and Wnt5a protein expressions in the majority of HCC patients （63% and 77%, respectively） was significantly less in tumor tissues, as compared to adjacent nontumorous tissues, and this reduction was correlated with increasing serum α-fetoprotein and tumor stage. In 68% （58/85） of the HCC cases, the expression of IB-catenin in tumor tissues was either downregulated in the cellular membrane, upregulated in the cytoplasm, or both. Survival analysis indicated that Wnt5a and Ror2 protein expressions could be regarded as independent prognostic factors for HCC; HCC patients with decreased Wnt5a or Ror2 protein expression had a poorer progno

关键词： Hepatocellular carcinoma Wnt5a Receptortyrosine kinase-like orphan receptor 2 13-catenin Prog-nosis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A Review of Lignocellulosic Biomass Pretreatment Technologies

引用

Paper And Biomaterials 2021年第3期6卷 61-76页

作者： Caoxing Huang Jiao Liu Wenhui geng Wei Tang Qiang yong Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources College of Chemical EngineeringNanjing Forestry UniversityNanjingJiangsu Province210037China Department of Forest Biomaterials North Carolina State UniversityRaleighNC27695USA

Lignocellulose is the most abundant renewable resource on ***,owing to the tightly entangled structural characteristics,it is challenging to convert lignocellulose into bio-based products in the biorefinery process without *** can destroy the natural resistance structure of lignocellulosic biomass,which is conducive to its downstream enzymatic saccharification and fermentation ***,chemical,and physicochemical pretreatments have been widely conducted for lignocellulosic biomass;several updated approaches and peculiar chemicals have also been proposed for these pretreatment methods in the recent ***,this study comprehensively reviews the novel technologies and chemicals that were applied in the various *** addition,the mechanisms,advantages,and disadvantages of the updated pretreatments are discussed to provide a reference for developing new pretreatment methods.

关键词： lignocellulosic biomass pretreatment enzymatic hydrolysis bioethanol

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Periodic Wave Solutions for(2+1)-Dimensional Boussinesq Equation and(3+1)-Dimensional Kadomtsev-Petviashvili Equation

引用

Communications in Theoretical Physics 2008年第11期50卷 1169-1176页

作者： ZHANG Huan TIAN Bo ZHANG Hai-Qiang geng Tao MENG Xiang-Hua LIU Wen-Jun cai Ke-Jie School of Science P.O. Box 122 Beijing University of Posts and Telecommunications Beijing 100876 China State Key Laboratory of Software Development Environment Beijing University of Aeronautics and Astronautics Beijing 100083 China Key Laboratory of Optical Communication and Lightwave Technologies Ministry of Education Beijing University of Posts and Telecommunications Beijing 100876 China

For describing various complex nonlinear phenomena in the realistic world,the higher-dimensional nonlinearevolution equations appear more attractive in many fields of physical and engineering *** this paper,by virtueof the Hirota bilinear method and Riemann theta functions,the periodic wave solutions for the(2+1)-dimensionalBoussinesq equation and(3+1)-dimensional Kadomtsev-Petviashvili(KP)equation are ***,it isshown that the known soliton solutions for the two equations can be reduced from the periodic wave solutions.

关键词： periodic wave solutions (2+1)-dimensional Boussinesq equation (3+1)-dimensional KP equation Hirota bilinear method

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Interaction pathways of implant metal localized corrosion and macrophage inflammatory reactions

引用

Bioactive Materials 2024年第1期31卷 355-367页

作者： Meng Li Jing Wu Wenbo geng Pengfei Gao Yulu Yang Xuan Li Kun Xu Qiang Liao Kaiyong cai Key Laboratory of Biorheological Science and Technology Ministry of EducationCollege of BioengineeringChongqing UniversityChongqing400044PR China Key Laboratory of Low-grade Energy Utilization Technologies and Systems Chongqing UniversityMinistry of EducationSchool of Energy and Power EngineeringChongqing400044PR China

Macrophages play a central role in immunological responses to metallic species associated with the localized corrosion of metallic implants,and mediating in peri-implant ***,the pathways of localized corrosion-macrophage interactions were systematically investigated on 316L stainless steel(SS)implant *** monitoring under macrophage-mediated inflammatory conditions showed a decreased pitting corrosion resistance of 316L SSs in the presence of RAW264.7 cells as the cells would disrupt biomolecule adsorbed layer on the metal *** pitting potentials were furtherly decreased when the RAW264.7 cells were induced to the M1 pro-inflammatory phenotype by the addition of lipopolysaccharide(LPS),and pitting corrosion preferentially initiated at the peripheries of *** overproduction of aggressive ROS under inflammatory conditions would accelerate the localized corrosion of 316L SS around *** pitting corrosion condition,the viability and pro-inflammatory polarization of RAW264.7 cells were region-dependent,lower viability and more remarkable morphology transformation of macrophages in the pitting corrosion region than the pitting-free *** pitting corrosion of 316L SS induced high expression of CD86,TNF-α,IL-6 and high level of intracellular ROS in *** release of metallic species(Fe^(2+),Cr^(3+),Ni^(2+),etc)and uneven distribution of surface overpotential stimulated macrophage inflammatory responses near the corrosion pits.A synergetic effect of localized corrosion and macrophages was revealed,which could furtherly promote localized corrosion of 316L SS and macrophage inflammatory *** results provided direct evidence of corrosion-macrophage interaction in metallic implants and disclosed the pathways of this mutual stimulation effect.

关键词： Metallic implant Macrophage Localized corrosion Inflammatory reaction Material-cell interaction

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Therapeutic effect of nerve growth factor on cerebral infarction in dogs using the hemisphere anomalous volume ratio of diffusion-weighted magnetic resonance imaging

引用

Neural Regeneration Research 2012年第24期7卷 1873-1880页

作者： yong Wang Hui Zhang Zhe Wang Zuojun geng Huaijun Liu Haiqing Yang Peng Song Qing Liu Department of Radiology the Second Hospital of Hebei Medical UniversityShijiazhuang 050000Hebei ProvinceChina Department of Radiology Hebei General HospitalShijiazhuang 050051Hebei ProvinceChina Department of Computer Science Institute of Basic MedicineHebei Medical UniversityShijiazhuang 050031Hebei ProvinceChina

A model of focal cerebral ischemic infarction was established in dogs through middle cerebral artery occlusion of the right *** minutes after occlusion,models were injected with nerve growth factor adjacent to the infarct *** therapeutic effect of nerve growth factor against cerebral infarction was assessed using the hemisphere anomalous volume ratio,a quantitative index of diffusion-weighted *** 6 hours,24 hours,7 days and 3 months after modeling,the hemisphere anomalous volume ratio was significantly reduced after treatment with nerve growth factor. Hematoxylin-eosin staining,immunohistochemistry,electron microscopy and neurological function scores showed that infarct defects were slightly reduced and neurological function significantly improved after nerve growth factor *** result was consistent with diffusion-weighted MRI *** findings indicate that nerve growth factor can protect against cerebral infarction,and that the hemisphere anomalous volume ratio of diffusion-weighted MRI can be used to evaluate the therapeutic effect.

关键词： diffusion-weighted MRI nerve growth factor hemisphere anomalous volume ratio cerebral infarction treatment neuroprotection brain regeneration neural regeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Dual‑Atom Nanozyme Eye Drops Attenuate Inflammation and Break the Vicious Cycle in Dry Eye Disease

引用

Nano-Micro Letters 2024年第6期16卷 439-457页

作者： Dandan Chu Mengyang Zhao Shisong Rong Wonho Jhe Xiaolu cai Yi Xiao Wei Zhang Xingchen geng Zhanrong Li Xingcai Zhang Jingguo Li Henan Eye Hospital Henan Provincial People’s HospitalPeople’s Hospital of Zhengzhou UniversityZhengzhou 450003People’s Republic of China Department of Ophthalmology Mass Eye and EarMass General BrighamHarvard Medical SchoolBostonMA 02114USA School of Engineering and Applied Sciences Harvard UniversityCambridgeMA 02138USA College of Chemistry Zhengzhou UniversityZhengzhou 450001People’s Republic of China

Dry eye disease(DED)is a major ocular pathology worldwide,causing serious ocular discomfort and even visual *** incidence of DED is gradually increasing with the highfrequency use of electronic *** inflammation is core cause of the DED vicious cycle,reactive oxygen species(ROS)play a pivotal role in the vicious cycle by regulating inflammation from ***,current therapies merely targeting inflammation show the failure of DED ***,a novel dual-atom nanozymes(DAN)-based eye drops are *** antioxidative DAN is successfully prepared by embedding Fe and Mn bimetallic single-atoms in N-doped carbon material and modifying it with a hydrophilic *** in vitro and in vivo results demonstrate the DAN is endowed with superior biological activity in scavenging excessive ROS,inhibiting NLRP3 inflammasome activation,decreasing proinflammatory cytokines expression,and suppressing cell ***,the DAN effectively alleviate ocular inflammation,promote corneal epithelial repair,recover goblet cell density and tear secretion,thus breaking the DED vicious *** findings open an avenue to make the DAN as an intervention form to DED and ROSmediated inflammatory diseases.

关键词： Dry eye disease DAN Dual-atom nanozyme Vicious cycle NLRP3 inflammasome Nanomedicine

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Two new species of Hartigia Schidte from China(Hymenoptera:Cephidae)

引用

Zoological Systematics 2015年第2期40卷 223-227页

作者： geng-Yun Niu Wei Xiao Mei-cai Wei Key Laboratory of Cultivation and Protection for Non–Wood Forest Trees(Central South University of Forestry and Technology) Ministry of Education

Two new species of Hartigia Schiodte, 1838 from China are described： H. maculothoracica sp. nov. from Xinjiang and H. fuscicosta sp. nov. from Jilin. A diagnosis of Hartigia and a key to Hartigia maculothoracica and ... 详细信息

关键词： Cephidae Hartigiinae Hartigia new species China.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Low-frequency transcranial magnetic stimulation is beneficial for enhancing synaptic plasticity in the aging brain

引用

Neural Regeneration Research 2015年第6期10卷 916-924页

作者： Zhan-chi Zhang Feng Luan Chun-yan Xie Dan-dan geng Yan-yong Wang Jun Ma Department of Human Anatomy Hebei Medical University Department of Otorhinolaryngology Third Hospital of Hebei Medical University Second Surgical Department Qinghe Public Hospital of Hebei Province Department of Neurology First Hospital of Hebei Medical University Hebei Key Laboratory for Brain Aging and Cognitive Neuroscience

In the aging brain, cognitive function gradually dedines and causes a progressive reduction in the structural and functional plasticity of the hippocampus. Transcranial magnetic stimulation is an emerging and novel neurological and psychiatric tool used to investigate the neurobiology of cognitive function. Recent studies have demonstrated that low-frequency transcranial magnetic stimulation （〈1 Hz） ameliorates synaptic plasticity and spatial cognitive deficits in learning-impaired mice. However, the mechanisms by which this treatment improves these deficits during normal aging are still unknown. Therefore, the current study investigated the effects of transcranial magnetic stimulation on the brain-derived neurotrophic factor signal pathway, synaptic protein markers, and spatial memory behavior in the hippocampus of normal aged mice. The study also investigated the downstream regulator, Fyn kinase, and the downstream effectors, synaptophysin and growth-associated protein 43 （both synaptic markers）, to determine the possible mechanisms by which transcranial magnetic stimulation regulates cognitive capacity. Transcranial magnetic stimulation with low intensity （110% average resting motor threshold intensity, 1 Hz） increased mRNA and protein levels of brain-derived neurotrophic factor, tropomyosin receptor kinase B, and Fyn in the hippocampus of aged mice. The treatment also upregulated the mRNA and protein expression of synaptophysin and growth-associated protein 43 in the hippocampus of these mice. In conclusion, brain-derived neurotrophic factor signaling may play an important role in sustaining and regulating structural synaptic plasticity induced by transcranial magnetic stimulation in the hippocampus of aging mice, and Fyn may be critical during this regulation. These responses may change the structural plasticity of the aging hippocampus, thereby improving cognitive function.

关键词： neural regeneration non-invasive brain stimulation transcranial magnetic stimulation neurotrophic factor brain-derived neurotrophic factor neuroplasticity hippocampus aging cognitive function

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Ultramicro molybdenum nitride powder prepared using high-energy mechanochemical method

引用

Rare Metals 2008年第3期27卷 303-307页

作者： AN geng LIU Gaojie Technical Center Jinduicheng Molybdenum Co. Ltd Xi＇an 710075 China School of Materials Science and Engineering Xi＇an Jiaotong University Xi＇an 710049 China

Using the specially designed mechanochemical ball-mill equipment, ultramicro molybdenum nitride powders were prepared from pure molybdenum powders in ammonia atmosphere at room temperature by high-energy ball milling. The structure and the particle size of the powders were investigated by X-ray diffraction （XRD）, scanning electron microscopy （SEM）, and transmission electron microscopy （TEM）. The results show that the mass ratio of grinding media to powder was 8：1, after milling for 30 h the Mo2N of fcc structure was obtained, and the average particle size of the powders was around 100 nm. It is found that the chemisorption of ammonia onto the fresh molybdenum surfaces created by milling was the predominant process during solid-gas reaction, and the energy input due to introduction of highly dense grain boundaries and lattice defects offered the activation energy for the transition from Mo-N chemisorption to molybdenum nitride. In addition, the change of Mo electronic undersaturation induced by the grain refining accelerated the bonding between Mo and N. The mechanism model of whole nitriding reaction was given, During the high-energy ball milling processing, the rotational speed of milling played a critical role in determining the overall reaction speed.

关键词： material preparation molybdenum nitride mechanochemical method ultramicro powder

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The crystal structure of LidA,a translocated substrate of the Legionella pneumophila type IV secretion system

引用

Protein & Cell 2013年第12期4卷 897-900页

作者： geng Meng Xiaojing An Sheng Ye yong Liu Wenzhuang Zhu Rongguang Zhang Xiaofeng Zheng State Key Lab of Protein and Plant Gene Research School of Life SciencesPeking UniversityBeijing 100871China Department of Biochemistry and Molecular Biology School of Life SciencesPeking UniversityBeijing 100871China National Laboratory of Biomacromolecules Institute of BiophysicsChinese Academy of SciencesBeijing 100101China

Dear Editor,Legionella pneumophila,a gram-neg-ative bacterium,is the causative agent of Legionnaires’pneumonia by infecting human lung macrophages(Muder et al.,1986).Upon uptake into macrophages,the bacteria are internalized to escape the endocytic pathway and lysosomal network,and establish the Legionella-containing vacuole(LCV)as an intracel-lular replicative organelle(Roy et al.,1998).The LCV hijacks and recruits the early secretory ER-derived vesicles and transforms them into ER-like *** the specialized ER-like vacuole,*** replicates to a high den-sity and subsequently kills the host cell by lysing the host cell *** released bacteria can infect neighboring cells to initiate a new round of infection(Tilney et al.,2001).

关键词： agent system specialized

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：