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Lithium inhibits proliferation of human esophageal cancer cell line Eca-109 by inducing a G_2/M cell cycle arrest

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World Journal of Gastroenterology 2008年第25期14卷 3982-3989页

作者： jin-Sheng wang Cui-Lian wang Ji-Fang Wen yong-jin wang yong-Bin Hu Hong-Zheng Ren Department of Pathology Xiangya Medical CollegeCentral South University Changsha 410008 Hunan ProvinceChina Department of Pathology Changzhi Medical College Changzhi046000 Shanxi Province China

AIM： To investigate the effect of lithium on proliferation of esophageal cancer （EC） cells and its preliminary mechanisms. METHODS： Eca-109 cells were treated with lithium chloride, a highly selective inhibitor of glycogen synthase kinase 31）（GSK-31））, at different concentrations （2-30 mmol/L） and time points （0, 2, 4, 6 and 24 h）. Cell proliferative ability was evaluated by 3-（4,5-Dimethylthiazol-2-yl）-2, 5-diphenyltetrazolium bromide （MTT） assay, and Cell cycle distribution was examined by flow cytometry. Expressions of p-GSK-3β, β-catenin, cyclin B1, cdc2 and cyclin D1 protein were detected by Western blotting, and the subcellular localization of β-catenin was determined by immunofluorescence. The mRNA level of of cyclin B1 was detected by reverse transcription polymerase chain reaction （RT-PCR）. RESULTS： Lithium could inhibit the proliferation of Eca-109 cells. Lithium at a concentration of 20 mmol/L lithium for 24 h produced obvious changes in the distribution of cell cycle, and increased the number of cells in G2/M phase （P 〈 0.05 vs control group）. Western blotting showed that lithium inhibited GSK-β） by Ser-9 phosphorylation and stabilized free β-catenin in the cytoplasm. Immunofluorescence further confirmed that free β-catenin actively translocated to the nucleus. Horeover, lithium slightly elevated cyclin D1 protein expression, whereas lowered the cyclin B1 expression after 24 h lithium exposure and no obvious change was observed for cdc2 protein. CONCLUSION： Lithium can inhibit the proliferation of human esophageal cancer cell line Eca-109 by inducing a G2/H cell cycle arrest, which is mainly mediated through the inhibition of lithium-sensitive molecule, GSK-3β, and reduction of cyclin B1 expression.

关键词： Lithium Esophageal cancer Cell cycle Glycogen synthase kinase 3β

From Markov Jump Systems to Two Species Competitive Lotka-Volterra Equations with Diffusion

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Acta Mathematica Sinica,English Series 2009年第1期25卷 157-170页

作者： Xue Qiang wang Li Jun BO yong jin wang School of Mathematical Sciences and LPMC Nankai University Tianjin 300071 P. R. China Department of Mathematics Xidian University Xi'an 710071 P. R. China

In this paper, we start at a random evolution system on biological particles, which is described by a Markov jump system. Under a suitable scaling, we perform a proper approximation procedure. Then the so-called weak convergence of Markov processes and Martingales allow us to establish a （deterministic） two species competitive Lotka-Volterra equation.

关键词： Markov jump system competitive Lotka-Volterra equation weak convergence

Phase Transformation and Carbide Precipitation of Functional Gradient Semi-solid 9Cr18 Steel

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Acta Metallurgica Sinica(English Letters) 2018年第8期31卷 823-830页

作者： yong-jin wang Ren-Bo Song Ren-Feng Song School of Materials Science and Engineering University of Science and Technology BeijingBeijing 100083China Institute of Industrial Science The University of TokyoKomaba 4-6-1MeguroTokyo 153-8505Japan Ansteel Mining Engineering Corporation Anshan 114004China

The unique phase transformation and carbide evolution in 9Cr18 steel were investigated during semi-solid forming and subsequent heat treatment. The functional gradient thixoforging 9Cr18 component was divided into inner area and edge area. Microstructure evolution was different at each area. After semi-solid cooling, the solid particles in the inner area were retained as meta-austenite. During annealing, M_（23）C_6 carbide began to precipitate when temperature reached 700 °*** transformation occurred when temperature reached 800 °C. The occurrence of M_（23）C_6 carbide and martensite structure would be harmful to the mechanical properties of inner area. In the edge area, the liquid underwent eutectic transformation to form bar-shape M_7C_3 carbide and secondary austenite after semi-solid cooling. The width of bar-shape carbide would decrease during annealing. By controlling the carbide evolution, we could tailor the functional gradient material with required property.

关键词： Semi-solid forming Solid/liquid phase Annealing Carbide precipitation

Constitutive Modeling for Thixoforming of 9Cr18 Semi-solid Alloy and 3D Forecast Mapping

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Journal of Iron and Steel Research International 2016年第12期23卷 1309-1315页

作者： Ya-ping LI Ren-bo SONG yong-jin wang School of Materials Science and Engineering University of Science and Technology Beijing

Thixotropic Compression tests were carried out on 9Cr18 semi-solid alloy through Gleeble-1500 thermal simulation machine. According to the experiment analysis, macro separation occurred during thixoforming. The liquid film was extruded outside to the surface and solidified to form eutectic structure. The solid particles were connected with each other and underwent plastic deformation. According to the comparison between Zhou-Guan model and modified Zhou-Guan model, it could be observed that the adding of thixotropic factor played an important role in the regression and the latter one was more credible. The modified Zhou-Guan model could well describe the thixoforming behavior. 3D forecast mapping was built for 9Cr18 semi solid alloy in thixoforming temperature range. It would provide valuable information for selecting process parameters during thixoforming in the manufacture process.

关键词： thixoforming constitutive model liquid fraction thixotropic factor 3D forecast

Hot ductility behavior of a Fe-0.3C-9Mn-2Al medium Mn steel

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International Journal of Minerals,Metallurgy and Materials 2021年第3期28卷 422-429页

作者： yong-jin wang Shuai Zhao Ren-bo Song Bin Hu School of Materials Science and Engineering University of Science and Technology BeijingBeijing 100083China School of Metallurgical and Ecological Engineering University of Science and Technology BeijingBeijing 100083China

The hot ductility of a Fe-0.3C-9Mn-2Al medium Mn steel was investigated using a Gleeble3800 thermo-mechanical *** tensile tests were conducted at different temperatures(600-1300℃)under a constant strain rate of 4×10^(−3)s^(−1).The fracture behavior and mechanism of hot ductility evolution were *** showed that the hot ductility decreased as the temperature was decreased from 1000℃.The reduction of area(RA)decreased rapidly in the specimens tested below 700℃,whereas that in the specimen tested at 650℃was lower than 65%.Mixed brittle-ductile fracture feature is reflected by the coexistence of cleavage step,intergranular facet,and dimple at the *** fracture belonged to ductile failure in the specimens tested between 720-1000℃.Large and deep dimples could delay crack *** change in average width of the dimples was in positive proportion with the change in *** wide austenite-ferrite intercritical temperature range was crucial for the hot ductility of medium Mn *** formation of ferrite film on austenite grain boundaries led to strain *** point elongation occurred at the austenite-ferrite intercritical temperature range during the hot tensile test.

关键词： medium Mn steel hot ductility reduction of area fracture behavior microstructure characterization

Large Deviation Principle for the Fourth-order Stochastic Heat Equations with Fractional Noises

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Acta Mathematica Sinica,English Series 2010年第1期26卷 89-106页

作者： Yi Ming JIANG Ke Hua SHI yong jin wang School of Mathematical Sciences and LPMC Nankai University Tianjin 300071 P. R. China

In this paper, we shall study a fourth-order stochastic heat equation driven by a fractional noise, which is fractional in time and white in space. We will discuss the existence and uniqueness of the solution to the equation. Furthermore, the regularity of the solution will be obtained. On the other hand, the large deviation principle for the equation with a small perturbation will be established through developing a classical method.

关键词： fourth-order stochastic heat equation fractional noise existence and uniqueness regularity large deviation principle

Effects of Temperature and Strain Rate on Solid-/Liquid-Phase Flow Behavior of 9Cr18 Steel During Thixoforging

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Acta Metallurgica Sinica(English Letters) 2017年第6期30卷 567-575页

作者： yong-jin wang Ren-Bo Song Ya-Ping Li Qiang Chen School of Materials Science and Engineering University of Science and Technology BeijingBeijing 100083China Mining Design and Research Institute of Anshan Iron&Steel Groups Corporation Anshan Anshan 114002China

A thixoforging process of the 9Cr18 steel was conducted in a designed setup, and a kind of multi-diameter component was fabricated. The effects of the forming temperature and the strain rate on the solid-/liquid-phase flow behavior were discussed. The results showed that functional gradient properties of the 9Cr18 steel could be obtained after thixoforging. Changes of microstructure along radial direction could be obtained. Solid austenite was retained after fast cooling, and the liquid film enriched in alloying elements was extruded outside to form a dendrite skin layer. As temperature increased, more molten liquid formed during thixoforging. A heterogeneous flow phenomenon was activated as free liquid channels were formed. The macro-separation of solid and liquid phases was critical for the formation of functional gradient properties. Above 1300 ℃, full dendrite skin layer could be formed. The strain rate affected the thixotropic property via influencing the deformation time of thixoforging. In the presence of lower strain rates, there was more time for the flow of liquid metal, which was the key to the extension of the thixotropic stage. High temperatures and low strain rates contributed to the formation of full skin layer for the designed specimen. The average thickness of skin layer for current specimen could be over 1000 ktm when thixoforged at 1340 ℃ and under a strain rate of 0.02 s^-1.

关键词： 9Cr18 steel Thixoforging Processing parameters Functional gradient property

Identification,Characterization, and Expression of P450 Gene Encoding CYP6BQ13v2 from the Red Flour Beetle,Tribolium castaneum (Herbst) (Coleoptera:Tenebrionidae)

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Agricultural Sciences in China 2009年第10期8卷 1210-1218页

作者： XU yong-qiang wang jin-jun JIANG Hong-bo DOU Wei TANG Pei-an AN Feng-ming Key Laboratory of Entomology and Pest Control Engineering College of Plant Protection Southwest University Chongqing 400716P.R. China

An allele of CYP6BQI3, named CYP6BQ 13v2 （GenBank accession no. FJ209361）, was isolated from the red flour beetle, Tribolium castaneum （Herbst）（Coleoptera： Tenebrionidae） by RT-PCR. The cDNA sequence of CYP6BQ13v2, 1 563 bp in length, contains an open reading frame of 1 554 nucleotides encoding a putative protein of 518 amino acid residues with a predicted molecular weight of 59.92 kDa and a theoretical pl of 7.60. The putative protein contains the classic hemebinding sequence motif F××G×××C×G （residues 456-465） conserved among all P450 enzymes as well as other characteristic motifs of all cytochrome P450s. It shares 98% identity with the previously published sequence of CYP6BQ13 （GenBank accession no. XP967146） from the T. castaneum genome project. Phylogenetic analysis of amino acid sequences from members of various P450 families indicated that there was closer phylogenetic relationship of CYP6BQ 13v2 with CYP302A1 and CYP307A1 mediating synthesis of the insect molting hormone, distant relationship with CYP6B1 metabolizing plant allelochemicals, CYP6D 1 linking to pyrethroid resistance and other members of CYP6 family. Expression test of the gene in the adults and immature stages of T. castaneum by quantitative real-time PCR revealed that CYP6BQ13v2 is expressed in all life stages investigated. The mRNA expression level in 1st instar larvae was 14.9- and 3.86-fold higher than those in pupae and adults, respectively. The CYP6BQ13v2 expression levels appeared in the order of 1st instar larvae, followed by 4th instar larvae, 7th instar larvae, adult, and pupae from high to low. The more bioinformation of CYP6BQ 13v2 was also analyzed.

关键词： Tribolium castaneum cytochrome P450 real-time PCR analysis bioinformatics

Orexin receptor type 2 agonism inhibits thermogenesis in brown adipose tissue by attenuating afferent innervation

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The Journal of Biomedical Research 2022年第3期36卷 195-207,I0005,I0006页

作者： Mo-qiu Jia yong-jin wang Kang Fu Han Jiao Jia Sun Yuanqing Gao Key Laboratory of Cardiovascular and Cerebrovascular Medicine School of PharmacyNanjing Medical UniversityNanjingJiangsu 211166China

Orexin signaling has been associated with energy expenditure and brown adipose tissue(BAT)***,conflicting data exist in the field about how orexin signaling regulates BAT *** this study,we show that a specific orexin receptor type 2(OX2R)agonist[Ala11,D-Leu15]-OxB(OB-Ala)inhibited intrascapular brown adipose tissue(iBAT)thermogenesis by reducing sympathetic output to *** effect is mediated by OX2Rs located on afferent nerve endings innervating iBAT instead of brown adipocyte *** of OB-Ala into iBAT inhibited iBAT thermogenesis in mice upon cold exposure and neuronal activity in the paraventricular *** suggest that OB-Ala could inhibit iBAT thermogenesis by attenuating sensory input thereby inhibiting the sympathetic-sensory iBAT feedback *** study uncovers a novel primary action site of orexin in the regulation of energy balance.

关键词： orexin receptor type 2 brown adipose tissue thermogenesis