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The construction of drug-resistant cancer cell lines by CRISPR/Cas9 system for drug screening

Science Bulletin 2018年第21期63卷 1411-1419页

作者： Lingmin Zhang Ying Li Qinghua Chen yong xia Wenfu Zheng Xingyu Jiang CAS Center for Excellence in Nanoscience Beijing Engineering Research Center for BioNanotechnologyCAS Key Laboratory for Biological Effects of Nanomaterials and NanosafetyNational Center for NanoScience and TechnologyBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China Guangdong Provincial Key Laboratory of Molecular Target &Clinical Pharmacology School of Pharmaceutical Sciences and the Third &Fifth Affiliated HospitalGuangzhou Medical UniversityGuangzhou 511436China

Cancer therapy is often hampered by the rapid emergence of drug resistance. Drug-resistant cellular models are essential for understanding the drug resistance and developing new therapeutics. The low efficiency and long time required in creating these models are major obstacles hindering drug resistance research and drug screening. Herein, we report an approach that can accelerate(shortening the time from years to 3 weeks) the establishment of cancer cell line-based, inheritable drug resistance models by specific knockout of MED12 gene using CRISPR/Cas9 system. The resultant MED12^(KO) A375(melanoma)cell line was resistant to inhibitors of B-Raf proto-oncogene, serine/threonine kinase(BRAF), whereas the resultant MED12^(KO) PC9(non-small cell lung cancer) cell line was resistant to inhibitors of epidermal growth factor receptor(EGFR). Evaluation of anti-cancer drugs and their combinations shows that certain combinations of BRAF inhibitors and TGF-β receptor(TGF-βR) inhibitors are active in suppressing the growth of MED12^(KO) A375 cells, and a few combinations of EGFR inhibitors and TGF-βR inhibitors were active in suppressing the growth of MED12^(KO) PC9 cells. The drug-resistant models will be useful in screening novel drugs and drug combinations for multi-drug-resistant cancer therapy.

关键词： CRISPR/Cas9 Drug resistance Cancer Cell line Gene editing

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A Comparison of Carlin-type Gold Deposits：Guizhou Province,Golden Triangle,Southwest China,and Northern Nevada,USA

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地学前缘 2013年第1期20卷 1-18页

作者： Jean S.CLlNE John L.MUNTEAN Xuexiang GU yong xia University of Nevada Las Vegas Nevada BareaH of Mines and Geology and University of Nevada Reno School of Earth Sciences and Resources China University of Geosiences(Beijing) State Key Laboratory of Ore Deposit Geochemistry.Institute of Geochemistry.Chinese Academy of Sciemces

Several Au deposits in Guizhou Province,southwest China,described as being similar to the highly productive Carlin-type gold deposits in northern ***,were examined to identify similarities and differences between the two *** were collected along transects from lowto high-grade rock,where possible,and fram stockpiles at the Shuiyindong,Zimudang,Taipingdong,Yata and Jinfeng(formerly Lannigou)*** used to examine ore and alteration minerals included hand-sample description:reflectance spectroscopy using an ASD Terraspec spectrometer;analyses of hand samples by carbonate staining with Alizaren red and potassium ferricyanide;transmitted and reflected light petrography;chemical analyses,mineral identification,and imaging using a ***-5610 scanning electron microscope:and quantitative chemical analyses using a JEOL JXA-8900 electron probe *** analyses of hand samples for 52 elements were done by ALS *** indicate both similarities and differences between the two *** districts have similar geologic *** deposits at both locations appear to have formed as a result of similar tectonic *** the district in southwest China lacks evidence of eoeval felsic igneous activity;however,the ore-stage minerals and the fluids that produced the minerals and deposits have some significant *** Nevada deposits were dominated by fluid-rock reaction in which host rock Fe was sulfidized to form Au-bearing *** ore fluids sulfidized host rock Fe in the Cuizhou *** timing of Fe metasomatism is unknown,so whether the deposits formed in response to sulfidation or pyritization is ***-rock reaction between an acidic,aqueous fluid and highly reactive calcareous rocks in Nevada caused extensive decarbonatization of host rocks,jasperoid replacemerit of carbonate minerals,and alteration of silty rock components to illite and *** Guizhou,CO2-bearing ore flu

关键词：地质地理学地质构造气藏

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Calculation of groundwater head distribution with a close barrier during excavation dewatering in confined aquifer

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Geoscience Frontiers 2021年第2期12卷 791-803页

作者： Hai-Min Lyu Shui-Long Shen yong-xia Wu An-Nan Zhou MOE Key Laboratory of Intelligent Manufacturing Technology Department of Civil and Environmental EngineeringCollege of EngineeringShantou UniversityGuangdongChina State Key Laboratory of Internet of Things for Smart City University of MacaoMacaoChina College of Mechanical and Automotive Engineering Shanghai University of Engineering ScienceShanghai201620China Civil and Infrastructure Discipline School of EngineeringRoyal Melbourne Institute of Technology(RMIT)MelbourneVic3001Australia

When pumping is conducted in confined aquifer inside excavation pit(waterproof curtain),the direction of the groundwater seepage outside the excavation changes from horizontal to vertical owing to the existence of the curtain *** is no analytical calculation method for the groundwater head distribution induced by dewatering inside *** paper first analyses the mechanism of the blocking effects from a close barrier in confined ***,a simple equation based on analytical solution is proposed to calculate groundwater heads inside and outside of the excavation pit with waterproof curtain(hereafter refer to close barrier)in a confined *** distribution of groundwater head is derived according to two conditions:(i)pumping with a constant water head,and(ii)pumping with a constant flow *** proposed calculation equation is verified by both numerical simulation and experimental *** comparisons demonstrate that the proposed model can be applied in engineering practice of excavation.

关键词： Confined aquifer Waterproof curtain Dewatering Groundwater head

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Effect of resilience on quality of life and anxiety in patients with breast cancer

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World Journal of Psychiatry 2024年第10期14卷 1458-1466页

作者： Ling-xia Kong yong-xia Yang Qian Zhao Zhi-Lin Feng Department of Breast Surgery The First Affiliated Hospital of Hebei North UniversityZhangjiakou 075000Hebei ProvinceChina Department of Psychiatry Zhangjiakou Shalingzi Hospital(Zhangjiakou Mental Health Center)Zhangjiakou 075200Hebei ProvinceChina

BACKGROUND The incidence of breast cancer is high,with serious implications in terms of lives and *** data show that there are approximately 1 million new cases of breast cancer reported annually,with a rising *** patients have poor treatment effects and are prone to anxiety and other negative emotions,which affect their quality of life(QoL).AIM To explore the correlation between mental resilience,QoL,and anxiety in patients with breast *** Using convenience sampling,200 patients with breast cancer were selected from the First Affiliated Hospital of Hebei North *** patients were investigated using the Conner-Davidson Resilience Scale,Self-Rating Anxiety Scale,and European Organization for Research and Treatment of Cancer Quality of Life Questionnaire to analyze the impact of resilience in patients with breast cancer on their QoL and *** The mean(SD)mental resilience score of the patients with breast cancer was 59.68(±9.84)points,the anxiety score was 49.87(±8.26)points,and the QoL score was 59.73(±8.29)***,they showed low mental resilience,mild anxiety,and medium *** was negatively correlated with mental resilience and QoL(r=-0.275,r=-0.289,P<0.05).QoL was positively correlated with mental resilience(r=0.513,P<0.05).Anxiety was a mediating variable between mental resilience and QoL,accounting for 8.58%of the mediating *** Regarding psychological elasticity,anxiety plays an intermediary role in QoL among patients with breast *** staff can improve patients'mental resilience by reducing their anxiety and improving their QoL.

关键词： Breast cancer Mental resilience Quality of life Anxiety Psycho-oncology Mediation effect

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Single-molecule fluorescence imaging of membrane-bound proteins for studies of cell signal transduction

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Chinese Science Bulletin 2011年第11期56卷 1063-1067页

作者： YANG yong, xia Tie ZHANG Wei FANG xiaoHong Beijing National Laboratory for Molecular Sciences Key Laboratory of Molecular Nanostructures and Nanotechnology Institute of ChemistryChinese Academy of Sciences Beijing 100190 China

Fluorescence imaging of single molecules is becoming a powerful tool to examine biological processes at the molecular *** total internal reflection fluorescence microscopy (TIRFM),it has been possible to study the dynamic behavior of single molecules on living cell ***,we briefly review the application of TIRFM-based single-molecule imaging in studies of membrane receptors involved in signal ***,we discuss several examples of our own research on growth factor receptors,including TGF-β receptors,HER2,and EGFR,and speculate possible applications of this technique to investigate other cellular events occurring on or near the plasma-membrane.

关键词：细胞信号转导单分子膜荧光成像结合蛋白生长因子受体荧光显微镜单分子成像生物过程

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Effects of nano-sized Ag reinforcing particulates on the microstructure of Sn-0.7Cu solder joints

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International Journal of Minerals,Metallurgy and Materials 2009年第6期16卷 677-684页

作者： Feng Tai Fu Guo Zhi-dong xia yong-ping Lei Yao-wu Shi College of Materials Science and Engineering Beijing University of Technology Beijing 100124 China

Composite solders were prepared by mechanically dispersing different volumes of nano-sized Ag particles into the Sn-0.7Cu eutectic solder. The effects of Ag particle addition on the microstructure of Sn-0.7Cu solder joints were investigated. Besides, the effects of isothermal aging on the microstructural evolution in the interfacial intermetallic compound （IMC） layer of the Sn-0.7Cu solder and the composite solder reinforced with 1vol% Ag particles were analyzed, respectively. Experimental results indicate that the growth rate of the interfacial IMC layer in the Ag particles reinforced composite solder joint is much lower than that in the Sn-0.7Cu solder joint during isothermal aging. The Ag particles reinforced composite solder joint exhibits much lower layer-growth coefficient for the growth of the IMC layer than the corresponding solder joint.

关键词： lead-free solder composite solder intermetallic compounds microstructure isothermal aging

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Modeling Climate Change Impacts on the US Agricultural Exports

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Journal of Integrative Agriculture 2014年第4期13卷 666-676页

作者： ZHANG Yu-quan CAI yong-xia Beach Robert H McCARL Bruce A Agricultural Resource & Energy Economics and Policy ProgramRTI InternationalResearch Triangle Park Department of Agricultural Economics Texas A&M UniversityCollege Station

Climate change is expected to have substantial effects on agricultural productivity worldwide. However, these impacts will differ across commodities, locations and time periods. As a result, landowners will see changes in relative returns that are likely to induce modifications in production practices and land allocation. In addition, regional variations in impacts can alter relative competitiveness across countries and lead to adjustments in international trade patterns. Thus in climate change impact studies it is likely useful to account for worldwide productivity effects. In this study, we investigate the implications of considering rest of world climate impacts on projections of the US agricultural exports. We chose to focus on the US because it is one of the largest agricultural exporters. To conduct our analyses, we consider four alternative climate scenarios, both with and without rest of world climate change impacts. Our results show that considering/ignoring rest of world climate impacts causes significant changes in the US production and exports projections. Thus we feel climate change impact studies should account not only for climate impacts in the country of focus but also on productivity in the rest of the world in order to capture effects on commodity markets and trade potential.

关键词： climate change relative competitiveness trade agricultural sector model (ASM)

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BLOCK ITERATIVE METHODS FOR LINEAR ALGEBRAIC EQUATION AND DOMAIN DECOMPOSITION METHOD FOR INCOMPRESSIBLE VISCOUS FLOW

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Journal of Hydrodynamics 2002年第4期14卷 73-80页

作者： Ren, An-Lu Zou, Jian-Feng Zhou, yong-xia Li, Guang-Wang Dept. of Mech. Zhejiang Univ. Hangzhou 310027 China

It was proved numerically that the Domain Decomposition Method CDDM) with one layer overlapping grids is identical to the block iterative method of linear algebra equations. The results obtained by using DDM could be in reasonable agreement with the results of full-domain simulation. With the three dimensional solver developed by the authors, the flow field in a pipe was simulated by using the full-domain DDM with one layer overlapping grids and with patched grids respectively. Both of the two cases led to the convergent solution. Further research shows the superiority of the DDM with one layer overlapping grids to the DDM with patched grids. A comparison between the numerical results obtained by the authors and the experimental results given by Enayet[3] shows that the numerical results are reasonable.

关键词： Computational fluid dynamics Computer simulation Iterative methods Linear equations Viscous flow

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Sandwich Structure Design of a Cooling Fin for Battery Modules Against Impact Loads

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Automotive Innovation 2020年第3期3卷 260-269页

作者： Zitong Shi Guanhua Chen Lingxiao Zhu Jie Li yong xia State Key Laboratory of Automotive Safety and Energy Tsinghua UniversityBeijing 100084China State Key Laboratory of Vehicle NVH and Safety Technology Chongqing 401120China

Lithium-ion batteries(LIBs)are widely employed in electric vehicles owing to their high power density,long cycle life,and environmental ***,LIBs are hazardous in the event of a crash,leading to thermal *** this study,the basic structure of a battery module is analyzed to improve the crashworthiness of LIBs.A simplified finite element model of the battery module structure,which is a battery unit composed of two pouch cells and a cooling fin,is set up and verified by conducting module-level *** simulation results reveal that the cooling fin in the battery module has the potential to absorb *** sandwich configurations are introduced to modify the cooling *** a unidirection-ally stiffened double hull USDH structure serving as an example,a parametric analysis is conducted,demonstrating that the sandwich height does not influence the areal density;a small height of 3 mm can make the material work sufficiently while avoiding early buckling of the ***,the crashworthiness of different sandwich configurations with the same areal density and height is compared,leading to three deformation *** and circular core structures are found to be able to effectively reduce the peak force and improve the energy absorption ability.

关键词： Lithium-ion battery Impact protection Sandwich configuration Cooling fin

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Simulation of EOM-based frequency-chirped laser slowing of MgF radicals

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Frontiers of physics 2022年第4期17卷 173-183页

作者： Kang Yan RuoXi Gu Di Wu Jin Wei yong xia Jianping Yin State Key Laboratory of Precision Spectroscopy School of Physics and Electronic ScienceEast China Normal UniversityShanghai 200241China NYU-ECNU Institute of Physics at NYU Shanghai Shanghai 200062China Collaborative Innovation Center of Extreme Optics Shanxi UniversityTaiyuan 030006China

Here we propose a scheme to slow MgF molecules by using EOM-based frequency-chirped radiation pressure *** scheme well addresses the need for a rapid chirp rate while light molecules are being laser slowed,whose scattering rate and recoil velocity are *** EOMs are used to compensate the rapidly changing Doppler shifts arised from the movement of molecules,and to cover the hyperfine energy structure of MgF,*** the scattering rate maps calculated from an optical Bloch equation model,individual molecule trajectories are simulated by using a semi-classical three-dimensional Monte Carlo *** show how the modulation configuration of EOM and the magnetic field influence the slowing *** study shows that a cryogenic buffer gas-cooled MgF beam source is possible to be slowed down with a number of∼1.4×10^(6)–10^(7),and the final forward speed peaks at∼10 m/s near the capture velocity of a molecular MOT.

关键词： laser cooling of molecule MgF molecule laser slowing dark state type-II transition

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