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Thioredoxin Reductase Type C （NTRC） Orchestrates Enhanced Thermotolerance to Arabidopsis by Its Redox-Dependent Holdase Chaperone Function

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Molecular Plant 2013年第2期6卷 323-336页

作者： Ho Byoung Chae Jeong Chan Moon Mi Rim Shin yong Hun chi Young Jun Jung Sun yong Lee Ganesh M. Nawkar Hyun Suk Jung Jae Kyung Hyun Woe Yeon Kim Chang Ho Kang Dae-Jin Yun Kyun Oh Lee Sang Yeol Lee Division of Applied Life Science (BK21 Program) and PMBBRC Gyeongsang National University 501 Jinju-daero Jinju 660-701 Korea Division of Electron Microscopic Research Korea Basic Science Institute 113 Gwahangno Yuse0ng-guDaejeon 303-333 Korea

Genevestigator analysis has indicated heat shock induction of transcripts for NADPH-thioredoxin reduc-tase, type C （NTRC） in the light. Here we show overexpression of NTRC in Arabidopsis （NTRC°E） resulting in enhanced tolerance to heat shock, whereas NTRC knockout mutant plants （ntrcl） exhibit a temperature sensitive phenotype. To investigate the underlying mechanism of this phenotype, we analyzed the protein＇s biochemical properties and protein structure. NTRC assembles into homopolymeric structures of varying complexity with functions as a disulfide reductase, a foldase chaperone, and as a holdase chaperone. The multiple functions of NTRC are closely correlated with protein structure.. Complexes of higher molecular weight （HMW） showed stronger activity as a holdase chaperone, while low molecular weight （LMW） species exhibited weaker holdase chaperone activity but stronger disulfide reductase and fol-dase chaperone activities. Heat shock converted LMW proteins into HMW complexes. Mutations of the two active site Cys residues of NTRC into Ser （C217/454S-NTRC） led to a complete inactivation of its disulfide reductase and foldase chaperone functions, but conferred only a slight decrease in its holdase chaperone function. The overexpression of the mutated C217/454S-NTRC provided Arabidopsis with a similar degree of thermotolerance compared with that of NTRC°E plants. However, after prolonged incubation under heat shock, NTRC°E plants tolerated the stress to a higher degree than C217/454S-NTRC°E plants. The results suggest that the heat shock-mediated holdase chaperone function of NTRC is responsible for the increased thermotolerance of Arabidopsis and the activity is significantly supported by NADPH.

关键词： NADPH-thioredoxin reductase type C (NTRC) oligomeric complexes disulfide reductase foldase and holdasechaperone functions redox thermotolerance.

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平头弹冲塞靶板的绝热剪切数值模拟

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爆炸与冲击 2011年第1期31卷 1-5页

作者：蒋东李永池中国科学技术大学近代力学系安徽合肥230027

针对绝热剪切形成时由于变形高度局域化,塑性功产生的热导致局部高温,有时会伴随动态再结晶(DRX)的现象,采用一种考虑动态再结晶过程的绝热剪切破坏准则,利用有限元方法模拟了Arne工具钢平头弹冲塞Weldox 460E钢靶板的实验。数值模拟揭... 详细信息

针对绝热剪切形成时由于变形高度局域化,塑性功产生的热导致局部高温,有时会伴随动态再结晶(DRX)的现象,采用一种考虑动态再结晶过程的绝热剪切破坏准则,利用有限元方法模拟了Arne工具钢平头弹冲塞Weldox 460E钢靶板的实验。数值模拟揭示了剪切带产生、传播的过程,温度分布情况表明在绝热剪切带中具备动态再结晶形成的物理条件。

关键词：爆炸力学绝热剪切有限元方法冲塞动态再结晶

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开展微创骨科技术之我见

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中华医学杂志 2006年第43期86卷 3025-3026页

作者：池永龙温州医学院附属第二医院骨科 325000

微创骨科并不是一门独立的新学科或新的分支学科,而是一种比传统骨科标准手术具有更小手术切口,更佳的内环境稳定,更轻的全身或局部反应,更快的骨组织愈合,更短的功能恢复时间和更好的心理效应的手术技术.手术是外科治疗的主要方式,各... 详细信息

微创骨科并不是一门独立的新学科或新的分支学科,而是一种比传统骨科标准手术具有更小手术切口,更佳的内环境稳定,更轻的全身或局部反应,更快的骨组织愈合,更短的功能恢复时间和更好的心理效应的手术技术.手术是外科治疗的主要方式,各种手术的方式和技术,无不凝结着外科医师们的心血.从人类的愿望和科学技术发展规律看,手术医师最终放弃各种对患者造成创伤的治疗方式,合理应用微创技术以最小的创伤达到最佳的疗效是手术医师的努力目标.要开展微创骨科技术,应维护患者达到最小的组织损伤,最轻的心身痛苦,最快的功能恢复,最少的治疗并发症,最廉的经济费用.并要求医生冲破传统观念束缚,勇于求新,积极探索和自我奉献的精神.同时要得到社会赞许,颂扬医疗职业道德,树立良好社会风气,建立良好医患关系,取得良好的社会效益[1]。

关键词：微创骨科科技手术切口技术发展规律良好医患关系手术医师治疗并发症分支学科

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面向现代化:我国普及普通话教育的战略思考

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教育科学 2008年第2期24卷 54-59页

作者：迟永长辽宁师范大学文学院辽宁大连116029

现代化对共同语提出迫切需求与国民普通话水平滞后的矛盾,国家大力推普与方言隔阂顽强延续的矛盾,是当代中国社会语言生活中最活跃的两大基本矛盾。这两对矛盾在运动中力量的消涨与发展走势影响着我国现代化的进程,解决好这些矛盾是摆... 详细信息

现代化对共同语提出迫切需求与国民普通话水平滞后的矛盾,国家大力推普与方言隔阂顽强延续的矛盾,是当代中国社会语言生活中最活跃的两大基本矛盾。这两对矛盾在运动中力量的消涨与发展走势影响着我国现代化的进程,解决好这些矛盾是摆在我国现代化面前的严肃课题。本文结合国情,就需求与现状,阻力与对策,机制与措施等与推普相关的诸多问题提出自己的思考意见。

关键词：现代化普通话方言隔阂普及教育

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热波移动对流向变换催化燃烧器运行特性影响的模拟研究

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动力工程学报 2014年第7期34卷 576-581,588页

作者：陈耿池涌宁波大学海运学院宁波315211 浙江大学能源清洁利用国家重点实验室杭州310027

对流向变换催化燃烧器的运行特性进行了数值模拟研究.选取热波移动距离作为比较的基准参数,研究了热波移动对燃烧器运行规律和熄火极限的影响.结果表明:当入口气速不同但热波移动距离相同时,在相同的入口反应物浓度下,燃烧器内温度分布... 详细信息

对流向变换催化燃烧器的运行特性进行了数值模拟研究.选取热波移动距离作为比较的基准参数,研究了热波移动对燃烧器运行规律和熄火极限的影响.结果表明:当入口气速不同但热波移动距离相同时,在相同的入口反应物浓度下,燃烧器内温度分布基本相似,入口气速越高,燃烧器内高温区越窄但最高温度升高;随着热波移动距离的增大,维持反应器自热运行所需的最低入口反应物浓度升高且增幅逐渐增大;采用高密度的惰性床料替换部分催化剂有利于降低熄火浓度,当惰性段装填比例约为20％～40％时,熄火浓度为570～710 mg/m3;随着惰性段装填比例的增大,相同操作条件下热波移动距离逐渐减小.

关键词：流向变换催化燃烧器热波低热值气体熄火极限数值模拟

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基于邻避型群体性事件的“价值—过程”框架构建与验证:来自公共价值视角的分析

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中国软科学 2019年第11期 64-73页

作者：钟俊弛马永驰浙江大学公共管理学院浙江杭州310058 大连理工大学公共管理系辽宁大连116024

邻避型群体性事件具有持续时间长、作用范围广、矛盾问题尖锐,妥善解决困难的特征,且近些年来我国邻避型群体性事件数量以每年29%的速度增长,处理好邻避型群体性事件成为我国治理现代化建设的关键因素。现有研究多从概念的构建、事件特... 详细信息

邻避型群体性事件具有持续时间长、作用范围广、矛盾问题尖锐,妥善解决困难的特征,且近些年来我国邻避型群体性事件数量以每年29%的速度增长,处理好邻避型群体性事件成为我国治理现代化建设的关键因素。现有研究多从概念的构建、事件特点分析、冲突原因的探索、治理策略进行分析,鲜有事件动态分析难以建立分析框架。本研究引入公共价值管理作为分析工具,对邻避型群体性事件危机周期的各个阶段进行动态过程诠释,建立并验证"价值-过程"分析框架,演绎此类事件的发生机理。

关键词：公共价值邻避性群体性事件框架构建

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FINITE ELEMENT ANALYSIS OF DDEFORMATION AND RESTORATION FOR CYLINDRICAL BEAM STRUCTURE

FINITE ELEMENT ANALYSIS OF DDEFORMATION AND RESTORATION FOR ...

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第十八届国际反应堆结构力学大会

作者： Myung Hwan Boo chi-yong Park Jin Won Kim Nuclear Mechanical ＆ Material Group KEPRI （Korea Electric Power Research Institute） Nuclear Mechanical ＆ Material Group KEPRI （Korea Electric Power Research Institute） Dept. of Nuclear Engineering Chosun University

＜正＞ 1 INTRODUCTION In plant industry, machine structure or machinery maintains its function by combination or co-relation of many structural parts. When a part of machine structure takes a severe damage by external forces, it can be exchanged by new parts. Before repair working process of the structure, integrity of damaged structure should be evaluated at deformed status. Also, after restoration by suitable work process, repaired structure has to be confirmed safety of structure. In this study, when a part of large size cylindrical structure is plastically deformed by external force, residual stress of structure was evaluated during deformation and restoration process by the finite element analysis.

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Biodiversity of freshwater ciliates(Protista,Ciliophora)in the Lake Weishan Wetland,china:the state of the

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Marine Life Science & Technology 2022年第4期4卷 429-451页

作者： Zhe Wang yong chi Tao Li Wenya Song Yunfeng Wang Tong Wu Gongaote Zhang Yujie Liu Honggang Ma Weibo Song Khaled A.S.Al-Rasheid Alan Warren Borong Lu Institute of Evolution and Marine Biodiversity Ocean University of ChinaQingdao 266003China Marine College Shandong UniversityWeihai 264209China Laoshan Laboratory Qingdao 266237China Zoology Department College of ScienceKing Saud UniversityRiyadh 11451Saudi Arabia Department of Life Sciences Natural History MuseumLondon SW75BDUK

Ciliates are core components of the structure of and function of aquatic microbial food *** play an essential role in the energy flow and material circulation within aquatic ***,studies on the taxonomy and biodiversity of freshwater ciliates,especially those in wetlands in china are *** address this issue,a project to investigate the freshwater ciliates of the Lake Weishan Wetland,Shandong Province,commenced in ***,we summarize our findings to date on the diversity of ciliates.A total of 187 ciliate species have been found,94 of which are identified to species-level,87 to genus-level,and six to *** species show a high morphological diversity and represent five classes,i.e.,Heterotrichea,Litostomatea,Prostomatea,Oligohymenophorea,and *** largest number of species documented are oligohymenophoreans.A comprehensive database of these ciliates,including morphological data,gene sequences,microscope slide specimens and a DNA bank,has been *** the present study,we provide an annotated checklist of retrieved ciliates as well as information on the sequences of published *** of these species are recorded in china for the first time and more than 20%are tentatively identified as new to ***,an investigation of environmental DNA revealed that the ciliate species diversity in Lake Weishan Wetland is higher than previously supposed.

关键词： Environmental DNA Protozoa Species diversity Taxonomy

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Causal roles of gut microbiota in cholangiocarcinoma etiology suggested by genetic study

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World Journal of Gastrointestinal Oncology 2024年第4期16卷 1319-1333页

作者： Zhi-Tao Chen Chen-Chen Ding Kai-Lei Chen Yang-Jun Gu chi-Cheng Lu Qi-yong Li Division of Hepatobiliary and Pancreatic Surgery Shulan(Hangzhou)Hospital Affiliated to Zhejiang Shuren University Shulan International Medical CollegeHangzhou 310000Zhejiang ProvinceChina Pediatric Psychology The Affiliated Mental Health Centre&Hangzhou Seventh People’s HospitalZhejiang University School of MedicineHangzhou 310000Zhejiang ProvinceChina School of Medicine Zhejiang Shuren UniversityHangzhou 310000Zhejiang ProvinceChina School of Medicine Zhejiang Chinese Medical University Zhejiang Shuren CollegeHangzhou 310000Zhejiang ProvinceChina

BACKGROUND Cholangiocarcinoma(CCA)is a highly malignant biliary tract cancer with poor *** studies have implicated the gut microbiota in CCA,but evidence for causal mechanisms is *** To investigate the causal relationship between gut microbiota and CCA *** We performed a two-sample mendelian randomization study to evaluate potential causal associations between gut microbiota and CCA risk using genome-wide association study summary statistics for 196 gut microbial taxa and *** variants were used as instrumental *** sensitivity analyses assessed result *** Fifteen gut microbial taxa showed significant causal associations with CCA *** genetically predicted abundance of genus Eubacteriumnodatum group,genus Ruminococcustorques group,genus Coprococcus,genus Dorea,and phylum Actinobacteria were associated with reduced risk of gallbladder cancer and extrahepatic *** intrahepatic CCA risk was associated with higher abundance of family Veillonellaceae,genus Alistipes,order Enterobacteriales,and phylum *** effects against CCA were suggested for genus Collinsella,genus Eisenbergiella,genus Anaerostipes,genus Paraprevotella,genus Parasutterella,and phylum *** analyses indicated these findings were reliable without *** This pioneering study provides novel evidence that specific gut microbiota may play causal roles in CCA *** experimental validation of these candidate microbes is warranted to consolidate causality and mechanisms.

关键词： Cholangiocarcinoma Mendelian randomization Gut microbiota Instrumental variables Sensitivity analyses

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Expression and self-assembly of Heterocapsa circularisquama RNA virus-like particles synthesized in Pichia pastoris

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Science Bulletin 2012年第25期 3289-3294页

作者： KIM Wonduck KIM Si-Wouk EOM chi-yong SHIN Hyun-Jae Department of Environmental Engineering Chosun UniversityGwangju 501-759Republic of Korea Seoul Center Korea Basic Science InstituteSeoul 136-713Republic of Korea Department of Chemical and Biochemical Engineering Chosun UniversityGwangju 501-759Republic of Korea

Heterocapsa circularisquama RNA virus(HcRNAV) is the first single-stranded RNA virus to be characterized that infects *** ability of HcRNAV coat protein(HcRNAV CP) to self-assemble into virus-like particles(VLPs) in vitro suggested that heterologous expression was possible,and that the VLPs might be ideal nanocontainers for the targeted delivery of genes and *** this paper,we report the expression of a codon-optimized HcRNAV 109 CP gene in Pichia pastoris and the production of self-assembled HcRNAV VLPs using large-scale *** HcRNAV 109 CP gene was synthesized according to the codon preference of *** and cloned into a pPICZA *** recombinant plasmid pPICZA-CPsyns was transformed into *** by *** resulting yeast colonies were screened by PCR and analyzed for protein expression by SDS polyacrylamide gel *** large-scale fermentation,the yield of HcRNAV CPsyns reached approximately 2.5 g L 1 within 4 *** HcRNAV VLPs were purified using PEG precipitation followed by cesium chloride density gradient ultracentrifugation,and were subsequently analyzed using UV spectrophotometry and transmission electron *** dye-labeled myoglobin was loaded into the cages of the HcRNAV VLPs and the encapsulation was confirmed by fluorescence *** results point to the possible utilization in pharmacology or nanotechnology of HcRNAV VLPs produced by *** fermentation.

关键词： Heterocapsa circularisquama RNA virus(HcRNAV) virus-like particles(VLPs) expression self-assembly Pichia pastoris

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