检索结果-南通市图书馆

Cultivating the unseen:Lessons from James Tiedje

在线全文

学校读者我要写书评

暂无评论

mLife 2023年第3期2卷 217-223页

作者： yoichi kamagata National Institute of Advanced and Industrial Science and Technology(AIST) TsukubaJapan

In recounting *** ***'s outstanding research achievements spanning the past 55 years,it is easy to overlook his early and mid-career ***,his contribution to the aerobic degradation of pesticides and other chemicals,as well as methanogenic degradation of those compounds retains their *** researchers in environmental microbiology have gained invaluable knowledge from these studies,which have been applied to the elucidation of previously uncultivated microorganisms.

关键词： cultivated gained pesticides

Effects of hydraulic retention time on nitrification activities and population dynamics of a conventional activated sludge system

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Environmental Science & Engineering 2013年第1期7卷 43-48页

作者： Hongyan LI Yu ZHANG Min YANG yoichi kamagata State Key Laboratory of Environmental Aquatic Chemistry Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China Research Institute of Genome-based Biofactory National Institute of Advanced Industrial Science and Technology Sapporo 062-8517 Japan

The effects of hydraulic retention time (HRT) on the nitrification activities and population dynamics of a conventional activated sludge system fed with synthetic inorganic wastewater were investigated over a period of 260 days. When the HRT was gradually decreased from 30 to 5 h, the specific ammonium-oxidizing rates (SAOR) varied between 0.32 and 0.45 kg (kg mixed liquor suspended solids (MLSS)·d) , and the specific nitrate-forming rates (SNFR) increased from 0.11 to 0.50 kg (kg MLSS·d) , showing that the decrease in HRT led to a significant increase in the nitrite oxidation activity. According to fluorescence in situ hybridization (FISH) analysis results, the proportion of ammonia-oxidizing bacteria (AOBs) among the total bacteria decreased from 33% to 15% with the decrease in HRT, whereas the fraction of nitrite-oxidizing bacteria (NOBs), particularly the fast-growing sp., increased significantly (from 4% to 15% for NOBs and from 1.5% to 10.6% for sp.) with the decrease in HRT, which was in accordance with the changes in SNFR. A short HRT favored the relative growth of NOBs, particularly the fast-growing sp., in the conventional activated sludge system.

关键词： ammonia-oxidizing bacteria hydraulic retention time nitrification activity nitrite-oxidizing bacteria population dynamics

New approaches for archaeal genome-guided cultivation

在线全文

学校读者我要写书评

暂无评论

Science China Earth Sciences 2021年第10期64卷 1658-1673页

作者： Yinzhao WANG yoichi kamagata Meng LI Feifei HAN Fengping WANG Xiang XIAO State Key Laboratory of Microbial Metabolism School of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghai 200240China Bioproduction Research Institute National Institute of Advanced Industrial Science and Technology(AIST)Tsukuba 310-8555Japan Shenzhen Key Laboratory of Marine Microbiome Engineering Institute for Advanced StudyShenzhen 518052China Department of Surgery Massachusetts General Hospital and Harvard Medical SchoolBoston MA 02114USA School of Oceanography Shanghai Jiao Tong UniversityShanghai 200240China State Key Laboratory of Ocean Engineering Ocean and Civil EngineeringShanghai Jiao Tong UniversityShanghai 200240China

Archaea,one of the three domains of life along with Bacteria and Eukarya,contains ancient life forms such as methanogen that are observed today on Earth,and one lineage Asgard archaea is also considered the closest ancestor of ***,with the development of interdisciplinary studies from Earth and Life sciences,archaeal organisms are considered to play pivotal roles in geochemical cycling in ***,our understanding of the attributes,origin and evolution,geochemical and ecological functions of Archaea is hampered by the scarcity of archaeal isolates,which has represented a challenge to researchers for the last 40 ***-independent sequencing and phylogenomic analyses have demonstrated a considerable diversity of Archaea with more than 20 novel ***,only four archaeal phyla have cultured representatives,leaving large gaps in our knowledge of the metabolic capabilities and ecological functions of the majority of archaeal strains identified exclusively by DNA *** this review,we summarize the discovery and development of archaeal research,highlight the knowledge gap between uncultured and cultured archaeal microbes,and call on the importance of devoting greater research efforts to archaeal ***,we outlined new ideas and strategic approaches,namely,(1)genome-based methods,(2)microbial network information-based methods,(3)genome-scale model-guided methods,and(4)machine learning methods,to enable the cultivation of uncultivated archaeal microbes using accumulated high-throughput sequencing data.

关键词： Archaea Cultivation Next-generation sequencing Uncultured archaea Genome Metabolic network

在线全文

学校读者我要写书评

暂无评论

基于基因组信息指导的古菌培养新策略

中国科学：地球科学 2021年第11期51卷 1837-1855页

作者：王寅炤 yoichi kamagata 李猛韩菲菲王风平肖湘上海交通大学生命科学技术学院微生物代谢国家重点实验室上海200240 Bioproduction Research Institute National Institute of Advanced Industrial Science and Technology(AIST)Tsukuba 310-8555Japan 深圳大学高等研究院深圳市海洋微生物组工程重点实验室广东518052 Department of Surgery Massachusetts General Hospital and Harvard Medical SchoolBoston MA 02114USA 上海交通大学海洋学院上海200240 上海交通大学船舶海洋与建筑工程学院海洋工程国家重点实验室上海200240

古菌、细菌及真核生物共同组成了地球上的三域生命体系.其中古菌域不仅包含了现今地球上最古老的生命类群,如产甲烷古菌;同时,阿斯加德(Asgard)古菌超门还被认为是真核生物的共同祖先.近些年来,随着地球科学和生命科学交叉研究日益加深... 详细信息

古菌、细菌及真核生物共同组成了地球上的三域生命体系.其中古菌域不仅包含了现今地球上最古老的生命类群,如产甲烷古菌;同时,阿斯加德(Asgard)古菌超门还被认为是真核生物的共同祖先.近些年来,随着地球科学和生命科学交叉研究日益加深,科学家发现古菌在地球化学元素循环中也起到了显著作用.通过不依赖于纯培养的高通量测序技术分析,表明古菌具有丰富的物种多样性,现已发现超过20个全新的古菌门.然而,目前仅在其中4个古菌门中获得了纯培养菌株,对古菌生理特征、起源与演化以及生态功能的认识也受到纯培养古菌菌株稀缺的限制,无法通过实验验证环境中未培养古菌基因组所预测到的功能基因.在本综述中,简述了古菌的发现与研究进展,对比了未培养和纯培养古菌之间的差距,并呼吁将更多的努力和科研资源投入到古菌分离和纯培养工作中.文章还概述了基于大量未培养古菌基因组指导分离和纯培养的新思路和新策略,即:基因组功能预测、代谢互作网络构建、基因组水平代谢建模、机器学习预测培养条件,期望能够使用现代高通量测序技术所积累的未培养古菌基因组数据来帮助获得更多新的纯培养古菌菌株.

关键词：古菌富集培养高通量测序未培养古菌基因组代谢网络

Symptomatic COVID-19 in University Students: A School-Wide Web-Based Questionnaire Survey during the Omicron Variant Outbreak

在线全文

学校读者我要写书评

暂无评论

Advances in Infectious Diseases 2024年第1期14卷 133-146页

作者： Mai Kitahara Hisami Sameshima Rie Tanuma Kumi Setoyama Yuka Yamaguchi Akiyo Kamachi Satoko Nakamura Mayuko Sakuma yoichi Kawaike Tamotsu Furuya Shinji Ijichi Health Service Center Kagoshima University Kagoshima Japan Center for Management of Information Technologies Kagoshima University Kagoshima Japan

Aim: To detect risk and preventive factors associated with the Omicron variant infection in university students, a combination of a web-based survey and multivariate logistic regression analysis was introduced as the front-line initiatives by the school health practitioners. Design: Questionnaire survey. Methods: The school-wide web-based questionnaire survey was conducted among our university students as a part of the annual health check-up in April, 2023. The positive outcome was confined to the first symptomatic COVID-19 onset during the Omicron variant outbreak. Results: In this self-administered survey, risk or protective associations were merely estimated statistically in university students (n = 5406). In measured factors, karaoke and club/group activities could maintain the statistical significance in adjusted odds ratios (ORs) as relative risk factors, and science course, measles/ rubella (MR) vaccination, and COVID-19 vaccination remained as relative protective factors in adjusted OR analyses. Club/group activities with member gathering and karaoke sing-along sessions in university students may frequently have WHO’s three Cs. These risk factors are still important topics for the infection control of COVID-19 in university students. Together with some recent reports from other researchers, the significant protective role of MR vaccine in our survey warrants further clinical investigation. If the breakthrough infection continuously constitutes the majority of infection, real data in test-negative case-control or web-based questionnaire design continue to be important for statistical analysis to determine the minimal requirement of our strategies which may be equivalent to or replace COVID-19 vaccines.

关键词： Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Coronavirus Disease 2019 (COVID-19) Omicron Variant Risk Behaviors Protective Factors

Now and future of mouse mutagenesis for human disease models

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2010年第9期37卷 559-572页

作者： yoichi Gondo Mutagenesis and Genomics Team RIKEN Bio Resource Center

One of the major objectives of the Human Genome Project is to understand the biological function of the gene and genome as well as to develop clinical applications for human diseases. For this purpose, the experimental validations and preclinical trails by using animal models are indispensable. The mouse （Mus musculus） is one of the best animal models because genetics is well established in the mouse and embryonic manipulation technologies are also well developed. Large-scale mouse mutagenesis projects have been conducted to develop various mouse models since 1997. Originally, the phenotype-driven mutagenesis with N-ethyl-N-nitrosourea （ENU） has been the major efforts internationally then knockout/conditional mouse projects and gene-driven mutagenesis have been following. At the beginning, simple monogenic traits in the experimental condition have been elucidated. Then, more complex traits with variety of environmental interactions and gene-to-gene interactions （epistasis） have been challenged with mutant mice. In addition, chromosomal substitution strains and collaborative cross strains are also available to elucidate the complex traits in the mouse. Altogether, mouse models with mutagenesis and various laboratory strains will accelerate the studies of functional genomics in the mouse as well as in human.

关键词： mutagenesis functional genomics reverse genetics model animal quantitative trait epistasis

Measurements of the Ca II infrared triplet lines of young stellar objects

在线全文

学校读者我要写书评

暂无评论

Research in Astronomy and Astrophysics 2013年第10期13卷 1189-1201页

作者： yoichi Itoh Keiko Moto'oka yoichi Itoh The Saga Prefecture Space & Science Museum 16351 Nagashima Takeo Takeo Saga 843-0021 Japan Nishi-Harima Astronomical Observatory Center for Astronomy University of Hyogo 407-2 Nishigaichi Sayo Hyogo 679-5313 Japan yitoh @nhao.jp

Equivalent widths and line widths of Ca II infrared triplet emission lines were measured in the high-resolution optical spectra of 39 young stellar *** found that the equivalent widths of the emission lines decrease with stellar *** has often been claimed that strong chromospheric activity is generated by a dynamo process caused by fast rotation of the ***,we found no clear correlation between the strength of the Ca II lines and the stellar rotation ***,we found that the objects with high mass accretion rates had stronger Ca II emission *** correlation supports the turbulent chromosphere model or the magnetic accretion theory for classical T Tauri *** also noticed that the equivalent widths of Ca II lines in transitional disk objects are one-tenth of those in classical T Tauri stars,even if the masses of the circumstellar disks are comparable.

关键词： stars:pre-main sequence stars:emission lines

Storage-efficient reconstruction framework for planar contours

在线全文

学校读者我要写书评

暂无评论

Geo-Spatial Information Science 2017年第1期20卷 14-28页

作者： Hiroyuki Goto yoichi Shimakawa Department of industrial and system engineering Hosei UniversitytokyoJapan Department of computer science and technology salesian polytechniquetokyoJapan

A storage-efficient reconstruction framework for cartographic planar contours is *** a smaller number of control points,we aim to calculate the area and perimeter as well as to reconstruct a smooth *** input data forms an oriented contour,each control point of which consists of three values:the Cartesian coordinates(x,y)and tangent angleθ.Two types of interpolation methods are developed,one of which is based on an arc spline while the other one is on a cubic Hermite *** arc spline-based method reconstructs a G1 continuous curve,with which the exact area and perimeter can be *** benefit of using the Hermite spline-based method is that it can achieve G2 continuity on most control points and can obtain the exact area,whereas the resulting perimeter is *** a numerical experiment for analytically defined curves,more accurate computation of the area and perimeter was achieved with a smaller number of control *** another experiment using a digital elevation model data,the reconstructed contours were smoother than those by a conventional method.

关键词： Oriented contour arc spline cubic Hermite spline geometric continuity storage efficient

Numerical analysis of surface plasmons excited on a thin metal grating

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2006年第1期7卷 55-70页

作者： OKUNO yoichi SUYAMA Taikei Graduate School of Science and Technology Kumamoto University Kumamoto 860-8555 Japan Department of Electrical and Computer Engineering Kumamoto University Kumamoto 860-8555 Japan

The authors numerically investigated the characteristics of surface plasmons excited on a thin metal grating placed in planer or conical mounting. After formulating the problem, the solution method, Yasuura＇s method （a modal expansion approach with least-squares boundary matching） was described. Although the grating is periodic in one direction, coupling between TE and TM waves Occurs because arbitrary incidence is assumed. This requires the employment of both TE and TM vector modal functions in the analysis. Numerical computations showed：（l） the excitation of surface plasmons with total or partial absorption of incident light; （2） the resonance character of the coefficient of an evanescent order that couples the plasmon surface wave; （3） the field profile and Poynting＇s vector. The plasmons excited on the surfaces of a thin metal grating are classified into three types： SISP, SRSP, and LRSP, different from each other in the feature of field profile and energy flow. In addition, the eigenvalue of a plasmon mode was obtained by solving a sequence of diffraction problems with complex-valued angles of incidence and using the quasi-Newton algorithm to predict the real angle of incidence at which the absorption occurs.

关键词： Thin metal grating Plasmon modes Resonance absorption Numerical analysis