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First report of Phytopythium vexans causing the“Avocado sadness”in Michoacan,Mexico

Phyton-International Journal of Experimental Botany 2019年第1期88卷 11-13页

作者： Hernandez Perez A E Cerna Chavez JC Delgado Ortiz M Beltran Beache LM Tapia Vargas ym ochoa fuentes Estudiante del postgrado Universidad Autonoma Agraria Antonio Narro Departamento de ParasitologiaBuenavistaSaltilloCoahuilaMexico CP 25315 Universidad Autonoma Agraria Antonio Narro Departamento de ParasitologiaBuenavistaSaltilloCoahuilaMexico CP 25315 Catedratico CONACYT-UAAAN.Universidad Autonoma Agraria Antonio Narro Departamento de ParasitologiaBuenavistaSaltilloCoahuilaMexico CP 25315 CULTA S.A.de C.V. InvestigacionDesarrolloAltavistaEl ManteTamaulipasMexico CP 898800 INIFAP Campo Experimental Uruapan.Av.Latinoamericana No.1101Col.Revolucion CP.60500UruapanMichoacanMexico

Mexico is the main producer,consumer and exporter of avocado in the world,being Michoacan the main producer state contributing more than 80%of the national *** are phytopathogens that decimate the production causing the death of the *** samples were collected in avocado trees that showed the characteristic symptomatology of the disease known as avocado sadness,the sampling was carried out in four of the main avocado producing towns,in the state of Michoacan,*** isolation consisted in sowing root tissue in Petri dishes with V8R-PARPH culture medium,subsequently they were identified morphologically and for species level it was determined by molecular biology,with the PCR-ITS *** tests were performed in triplicate with avocado seedlings with more than six *** 24 hours,the inoculated plants expressed decay in the apical part,after 120 hours the leaves showed yellowing and after 15 days there was a generalized wilt on the stem and leaves,re-isolating the phytopathogen Phytopythium *** study confirms the first report of the oomycete *** affecting avocado trees in the most important producing region of the Mexican Republic.

关键词： Molecular identification Pathogenicity Phytopythium vexans Avocado.

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Fast Detection of Auxins by Microplate Technique

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American Journal of Plant Sciences 2017年第2期8卷 171-177页

作者： Julia Cecilia Anguiano-Cabello Alberto Flores-Olivas Yisa María ochoa-fuentes Roberto Arredondo-Valdés Victor Olalde-Portugal Department of Parasitology Antonio Narro Agrarian Autonomous University Saltillo Mexico

Plant growth promotion indole-3-acetic acid (IAA) is the most abundant natural auxin that plays diverse roles in plant growth, development and plant immunity. Perturbing auxin homeostasis appears to be a common virulence mechanism, as many pathogens can synthesize auxin-like molecules. In other hand, the addition of plant growth promotion rhizobacteria (PGPR) that are able to produce auxins promotes plant growth and provides protection against pathogens. Techniques as high performance liquid chromatography (HPLC) and gas chromatography (GC) are used to quantify auxins produced by microorganism and plants at high precision and sensitivity, even though those techniques are expensive and require a big number of solvents. For these reasons, the aim of the present study was to develop a fast microplate technique for auxin detection, in Bacillus subtilis strains using salkowski reagent. For auxin quantification, calibration curves were done with alcohol, landy medium and water and the R2 were calculated. The microplate techniques were able to quantify auxin production by B. subtillis stains.

关键词： Auxin Microplate Bacillus subtilis

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Interaction Between Heficoverpa zea Damage with Corncob Diseases on Genetically Modified Corn in Sinaloa, México

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Journal of Life Sciences 2014年第4期8卷 329-334页

作者： Luis A. Aguirre Uribe Gustavo A. Frías Trevino Agustín Hernández Juárez Mariano Flores Davila Emesto Cerna Chavez Jerónimo Landeros Flores Yisa M. ochoa fuentes Plant Health Department Universidad A utónoma Agraria Antonio Narro Buenavista Saltillo 25315 Coahuila México

GM （genetically modified） corn with the CrylAb, mCry3A and Vip3Aa20 protein of Bt （Bacillus thuringiensis） and its respective isoline as control with and without chemical control for corn earworm were used in this research. Fusarium corncob damage was lower on GM corn with （12.5%） and without （25.7%） insecticide treatment as compared with the isoline that had 48.3% and 83.1% of damaged corncobs with and without chemical control, respectively. Corn smut cob damage was also lower on GM corn with （3.2%） and without （6.3%） insect control compared with 15.5% and 49.7% damage with and without insecticide treatment, respectively. Fusarium sp. corncob rot was also lower on GM corn with 5.7% and 9.5% whereas a 24.6% and 63% rot was observed on the isoline with and without insecticide control, respectively. Ustilago maydis severity was also lower on Bt corn finding 0.07% and 0.25% damage on treatments with and without insect control as compared with the isoline that showed an 11.6% and a 41.4% smut rot with and without insecticide treatment, respectively. The authors conclude that GM corn resistant to Helicoverpa zea prevents damage by the pest, eliminating the entrance pathway for Fusarium sp. and Ustilago maydis.

关键词： Bacillus thuringiensis transgenic Fusarium Ustilago maydis mycotoxins corn smut.

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First Report on the Presence of Phyllachora sp. in Corn Crops at Toluca, Estado de Mexico

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American Journal of Plant Sciences 2016年第5期7卷 733-739页

作者： Erika Natalia Ríos-Herrera Laila Pamela Partida-Martínez Yisa María ochoa-fuentes Ernesto Cerna-Chávez Francisco Daniel Hernández-Castillo Alberto Flores-Olivas Víctor Olalde-Portugal Raúl Rodríguez-Guerra Universidad Autónoma Agraria Antonio Narro Saltillo México Department of Genetic Engineering CINVESTAV Irapuato México Department of Agricultural Parasitology Universidad Autónoma Agraria Antonio Narro Saltillo México Department of Biotechnology and Biochemistry CINVESTAV Irapuato México General Terán INIFAP General Terán Nuevo León México

Symptoms of Tar Spot Complex (TSC) in corn appear in the form of black spots surrounded by a chlorotic halo known as fish-eye. The crop with these symptoms was found at San Pablo Autopan, Estado de Mexico. The stromal region of foliar lesions was cut longitudinally, showing perithecia, asci and ascospores typical of Phyllachora maydis. The results were confirmed by PCR-ITS with ITS 1 and ITS4 initiators, cloned by pJET, before being sequenced and compared against NCBI’s data base. Phyllachora identity was confirmed. This is the first report where TSC pathogen has been detected under conditions that had not been previously reported, for instance, at 2600 meters above the sea level.

关键词： Phyllachora maydis Tar Spot Complex ITS

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Marine turtle hatchlings use multiple sensory cues to orient their crawling towards the sea:biological and conservation policy implications

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Advances in Bioscience and Biotechnology 2011年第2期2卷 47-51页

作者： Alma Lilia fuentes-Farias Gabriel Gutierrez-Ospina Esperanza Melendez Herrera Veronica Camarena-Ramirez Gerardo ochoa-Tovar Julieta Mendoza-Torreblanca Armida Baez-Saldana Raquel Martinez-Mendez Jaime Urrutia-Fucugauchi Maria Luisa Garcia Zepeda Laboratorio de Ecofisiologia Facultad de BiologiaUniversidad Michoacana de San Nicolas de HidalgoCiudad UniversitariaMorelia MichoacanMexico58230 Laboratorio de Biologia de Sistemas Instituto de Investigaciones BiomedicasUniversidad Nacional Autonoma de MexicoCiudad UniversitariaMexico D.F.04510 Laboratorio de Paleomagnetismo Instituto de GeofisicaUniversidad Nacional Autonoma de MexicoCiudad UniversitariaMexico D.F.04510 Laboratorio de Paleontologia Facultad de BiologiaUniversidad Michoacana de San Nicolas de HidalgoCiudad UniversitariaMorelia MichoacanMexico58230 Laboratorio de Neuromorfometría Instituto Nacional de PediatríaMéxico D.F.04530

The ability of sea turtle hatchlings to find the seashore soon after hatching is thought to be exclusively dependent upon visual information. Target-oriented movements in most vertebrates, however, relay on combining information gathered through different sensory systems. Hence, in this work, we investigated whether olfactory and/or magnetic information might complement visual cues during hatchling’s seaward crawling. Acute olfactory deprivation and distorted magnetic sensation in visually competent hatchlings resulted in a scattering of seaward crawling routes among cardinal points, some of them being different from those strongly preferred by control hatchlings. In addition, blindfolded hatchlings also displayed a striking misrouting while crawling on the beach surface in spite of having intact olfactory and magnetic senses. Together these results support the notion that visual information is crucial for seaward crawling, but also that olfactory and magnetic information complement visual cues when turtle hatchlings display this behavior. Hence, the present observations suggest that multisensory cues are used by turtle hatchlings while crawling towards the sea. This work also has important implications on the design of species conservation measures and policies. In the near future, efforts must be made to identify and preserve the local natural sources of odors and magnetic cues, in addition to preventing the perturbing effects of artificial lighting on adult and hatchling turtle crawling behavior.

关键词： Olfaction Magnetoreception Vision Chelonia aggasizi Seaward orientation Reptiles Intermodal interactions Multisensory integration

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Anterior and Posterior Approach Results for Treatment of Cervical Myelopathy in the Elderly: A 10-Year Experience in a Mexican Institution

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Open Journal of Orthopedics 2021年第7期11卷 207-219页

作者： Mauricio Daniel Sánchez-Calderón María Elena Córdoba-Mosqueda José Ramón Aguilar-Calderón Carlos René Domínguez-Herz Diego ochoa-Cacique Daniel Alejandro Vega-Moreno Victor Andrés Reyes-Rodriguez Ulises García-González Abraham Ibarra-de la Torre Rodrigo Efraín Hernández-Reséndiz Department of Neurosurgery Hospital Central Sur de Alta Especialidad de Alta Especialidad de PEMEX Periferico sur 4091 Fuentes del Pedregal Tlalpan México City México Department of Neurosurgery Hospital Regional de Alta Especialidad “Centenario de la Revolución Mexicana” ISSSTE Cuernavaca Palo Escrito Emiliano Zapata Department of Neurosurgery Hospital Central Norte PEMEX Mexico City México Department of Neurosurgery Hospital &Aacute ngeles Clí nica Londres Mexico City Mexico

Introduction: Degeneration of the cervical spine (CDSD) prevalence is nearly 90% by the 7th decade. This is the first research that compares the outcomes between the Anterior Approach (AA) and Posterior Approach (PA) to cervical myelopathy (CM) in the elderly. Materials and Methods: A retrospective observational study of electronic health records at the Hospital Central Sur de Alta Especialidad (HCSAE), PEMEX from January 2010 to May 2020 with patients older than 60 years submitted to cervical surgery. For the analysis we elaborated two groups according to the surgical approach: AA vs PA;we analyzed the trans-operative behavior, the immediate outcome, and after 3 months, 6 months, and 1 year. Results: As a total of 145 patients, the prevalence of CM in elderly was the 63.8% with a median age of 69 (64 - 75) years. We found statistical differences in strength outcome only in the P3m (p = 0.011), for sensitivity we found major prevalence of affection in the PA group. We didn’t report a significant difference in the Neck Disability Index (NDI) at all measures, but the PA presented a major incapacity. The Nurick scale results were significant in all stages (p . Discussion: Patients show significant improvements in outcome measures with either anterior or posterior surgery. Both approaches are highly efficacious in preventing neurolo

关键词： Cervical Spine Surgical Approach Elderly Cervical Myelopathy Cervical Degenerative Spine Disease

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