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镀锡板无铬钝化工艺研究

轧钢 2021年第4期38卷 50-54页

作者：万一群缪军红孙宇沈鹏杰周纪名刘海涛首钢京唐钢铁联合有限责任公司河北唐山063210 东北大学轧制技术及连轧自动化国家重点实验室辽宁沈阳110819

基于钛锆-磷硅无铬钝化体系,采用正交试验方法,改变钝化液浓度、干燥温度和时间,成功获得了高性能无铬钝化膜,并确定了无铬钝化最佳工艺。通过测定试验样板的抗酸抗硫性能、涂漆附着力性能和电化学耐蚀性能,发现其综合性能接近铬酸盐钝... 详细信息

基于钛锆-磷硅无铬钝化体系,采用正交试验方法,改变钝化液浓度、干燥温度和时间,成功获得了高性能无铬钝化膜,并确定了无铬钝化最佳工艺。通过测定试验样板的抗酸抗硫性能、涂漆附着力性能和电化学耐蚀性能,发现其综合性能接近铬酸盐钝化产品。

关键词：镀锡板无铬钝化正交实验抗酸抗硫性能附着力

仿生组织工程软骨修复关节软骨损伤的效果及应用超声弹性成像方法检测价值分析

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中华老年骨科与康复电子杂志 2020年第2期6卷 62-67页

作者：马宁刘雨丰陈明学王月香万一群刘舒云眭翔郭全义解放军总医院骨科研究所北京100853

目的体外构建的仿生组织工程软骨修复羊膝关节软骨损伤,通过组织学染色、点压力学分析、超声弹性成像检查来评价修复效果,探讨超声弹性成像方法评估再生软骨质量的可行性.方法共选用雄性山羊12只,随机分A、B、C三组,A组空白对照组2只,... 详细信息

目的体外构建的仿生组织工程软骨修复羊膝关节软骨损伤,通过组织学染色、点压力学分析、超声弹性成像检查来评价修复效果,探讨超声弹性成像方法评估再生软骨质量的可行性.方法共选用雄性山羊12只,随机分A、B、C三组,A组空白对照组2只,仅做股骨髁负重区直径6 mm全层软骨缺损;B组单纯仿生软骨(ECM)支架修复组4只,在股骨髁负重区软骨缺损处仅植入仿生软骨支架;C组仿生软骨支架复合自体骨髓间充质干细胞(BMSCs)组6只.取自体髂骨骨髓血分离骨髓间充质干细胞培养,细胞浓度达到1×10^7个/毫升后加入仿生软骨支架中,将构建好的仿生组织工程软骨植入股骨髁软骨缺损中.每组的羊均先在左膝造模,术后3个月再右膝造模,待右膝术后3个月时先行超声弹性成像检查,然后取材行评分,病理染色、糖胺多糖(GAG)含量测定、力学检测分析.结果3个月和6个月的A组基本无软骨组织修复.在大体评分、病理评分、GAG含量测定、点压力学测试的结果显示C组优于B组(P<0.05),并且B组与C组中的6月数据均明显优于3个月(P<0.05).其中C组动物6个月取材发现重建软骨更接近正常软骨组织.修复组织大体形态结果,点压力学测试结果与超声弹性成像检查结果对比基本一致.结论利用组织工程方法构建仿生软骨可治疗早期软骨损伤,复合自体骨髓间充质干细胞的仿生支架能达到更好的修复效果.软骨损伤经过仿生软骨修复后恢复周期在6个月以上.超声弹性成像检查软骨修复效果可作为术后复查简单快捷有效的方法.

关键词：超声弹性成像技术软骨组织工程仿生支架

Primary dolostone formation related to mantle-originated exhalative hydrothermal activities,Permian Yuejingou section,Santanghu area, Xinjiang,NW China

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Science China Earth Sciences 2012年第2期55卷 183-192页

作者： LIU yiqun JIAO Xin LI Hong YUAN MingSheng YANG wan ZHOU XiaoHu LIANG Hao ZHOU DingWu ZHENG ChaoYang SUN Qin wanG ShuangShuang State Key Laboratory of Continental Dynamics Northwest UniversityDepartment of GeologyNorthwest UniversityXi'an 710069China Tu-Ha Oilfield CNPCHami 830002China Department of Geological Sciences and Engineering Missouri University of Science and TechnologyRollaMissouri 65409USA College of Geological Science&Engineering Shandong University of Science and TechnologyQingdao 266510China

The Permian Lucaogou Formation is an important hydrocarbon source rock in the Junggar, Turpan, and Santanghu basins in Xinjiang, NW China. For the first time, dolostones associated with mantle-originated exhalative hydrothermal fluid flows are discovered in Yuejingou section in the Santanghu Basin area. They include dolomicrite, doloarenite, and a small amount of dolorudite, and are finely (0.05-0.15 cm thick) interlaminated with lime micrite and dolomicritic analcime laminites. Alkali feldspar and analcime grains are common in doloarenite and are interpreted as having been derived from analcime phonolites and peralkaline magmatic rocks. These magmatic fragments were brought up from subsurface by hydrothermal fluid flow and had experienced exhalative brecciation, transport, and deposition on the lake floor. The matrix consists dominantly of dolomite and ankerite smaller than 0.01 mm. The dolostones can be subdivided into four types on the basis of mineral composition and content. The detrital analcime and alkaline feldspar grains and tuff lithics are interpreted as intraclasts, which were deposited in an under-filled starved lake basin. The δ18OPDB values of dolostones are -5‰ to -21.1‰, and -11.9‰ on average; the 87Sr/86Sr ratios of dolostones are 0.70457 to 0.706194, and 0.705005 on average. These values, in combination with evidence of multi-episodes of peralkaline extrusion, suggest a mantle origin of the hydrothermal fluids, which may have promoted primary dolomite formation. The fluid from the upper mantle caused serpentinization of ultramafic rocks that intruded into the lower crust to obtain Mg2+ and Fe2+, and injected the ions into the lake water as the Mg and Fe sources for dolomite and ankerite. Hydro- thermal fluids associated with peralkaline magmatic rocks also provided Ca2+, Mg2+, Fe2+, and CO32-. Explosive breccias formed and dolostones were convoluted near the vent of hydrothermal fluid exhalation, whereas laminated dolostones formed farther away fr

关键词： primary dolostone mantle-originated hydrothermal exhalative deposits intracontinental rift Permian Santanghu Area

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分光光度法测定石蜡相络合物的组成

南昌大学学报（理科版） 2000年第2期24卷 115-119页

作者：郭岚万益群戴超南昌大学化学系江西南昌330047

以镍 -丁二酮肟显色体系和钯 -α -亚硝基 - β -萘酚显色体系为例 ,对石蜡相中络合物组成比的测量原理和测定方法进行探讨 ,并测得镍与丁二酮肟石蜡相络合物的组成比以及钯与α-亚硝基 - β -萘酚石蜡相络合物的组成比均为 1∶2。

关键词：石蜡相络合物组成测定分光光度法显色体系

Arabidopsis Blue Light Receptor Phototropin 1 Undergoes Blue Light-Induced Activation in Membrane Microdomains

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Molecular Plant 2018年第6期11卷 846-859页

作者： yiqun Xue Jingjing Xing Yinglang wan Xueqin Lv Lusheng Fan Yongdeng Zhang Kai Song Li wang Xiaohua wang Xin Deng Frantisek Baluska John M. Christie Jinxing Lin Institute of Botany Chinese Academy of Sciences Beijing 100093 China University of Chinese Academy of Sciences Beijing 100049 China Department of Botany and Plant Sciences University of California Riverside CA 92521 USA Department of Cell Biology Yale University School of Medicine New Haven CT 06520 USA Institute of Cellular and Molecular Botany University of Bonn 53115 Bonn Germany Institute of Molecular Cell and Systems Biology University of Glasgow Bower Building Glasgow G12 8QQ UK College of Biological Sciences and Biotechnology Beijing Forestry University Beijing 100083 China

Phototropin （phot）-mediated signaling initiated by blue light （BL） plays a critical role in optimizing photosyn- thetic light capture at the plasma membrane （PM） in plants. However, the mechanisms underlying the regu- lation of phot activity at the PM in response to BL remain largely unclear. In this study, by single-particle tracking and stepwise photobleaching analysis of photl-GFP proteins we demonstrated that in the dark photl proteins remain in an inactive state and mostly exist as monomers. Dimerization and the diffusion rate of photl-GFP increased in a dose-dependent manner in response to BL. In contrast, BL did not affect the lateral diffusion of kinase-inactive photlD806N-GFP but did enhance its dimerization, suggesting that photl dimerization is independent of phosphorylation. Forster resonance energy transfer-fluorescence life- time imaging microscopy analysis revealed that the interaction between photl-GFP and a marker of sterol- rich lipid environments, AtRem1.3-mCherry, was enhanced with increased time of BL treatment. However, this BL-dependent interaction was not obvious in plants co-expressing phot1D806N-GFP and AtRem1.3- mCherry, indicating that BL facilitates the translocation of functional photl-GFP into AtRem1.3-1abeled microdomains to activate phot-mediated signaling. Conversely, sterol depletion attenuated photl-GFP dynamics, dimerization, and phosphorylation. Taken together, these results indicate that membrane micro- domains act as organizing platforms essential for the proper function of activated photl at the PM.

关键词： photl VA-TIRFM spatiotemporal dynamics blue light signaling membrane microdomains

固液萃取分离分光光度法测定痕量锰(Ⅱ)的研究

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南昌大学学报（理科版） 2000年第2期24卷 111-114页

作者：万益群赵风瑛杨立强南昌大学化学系江西南昌330047 南方工业学校江西赣州341000 江西广播电视大学江西南昌330046

描述了石蜡萃取分光光度测定痕量锰过程。在 pH =8.5～ 10 .0范围内 ,锰和 1- ( 2 -吡啶偶氮 ) - 2 -萘酚显色络合物能定量被石蜡萃取 ,将萃取的固相体系溶解在CHCl3中 ,在λ =570nm处进行光度测定。锰量在 0～ 10 μg/10mL范围内符合... 详细信息

描述了石蜡萃取分光光度测定痕量锰过程。在 pH =8.5～ 10 .0范围内 ,锰和 1- ( 2 -吡啶偶氮 ) - 2 -萘酚显色络合物能定量被石蜡萃取 ,将萃取的固相体系溶解在CHCl3中 ,在λ =570nm处进行光度测定。锰量在 0～ 10 μg/10mL范围内符合比耳定律 ,线性回归方程为 y =0 .0 0 32 +0 .0 4 86x。方法应用于实际样品中痕量锰测定。

关键词：固液萃取分光光度法锰显色体系测定 PNA

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石蜡相分光光度法连续测定铅铋的研究

南昌大学学报（理科版） 2000年第1期24卷 77-80页

作者：许逸敏万益群徐招娣郭岚南昌大学化学系江西南昌330047

研究了Pb一双硫腙及Bi一双硫腙显色体系在石蜡相的显色反应。Pb2 + 一双硫腙显色络合物在NH4 Cl-NH3(pH =10 )介质中可定量地被石蜡萃取 ,而Bi3+ 一双硫腙显色络合物在NH4 AC -HAC(pH =5)介质中可定量被石蜡萃取 ,两者固相色阶明显。铅... 详细信息

研究了Pb一双硫腙及Bi一双硫腙显色体系在石蜡相的显色反应。Pb2 + 一双硫腙显色络合物在NH4 Cl-NH3(pH =10 )介质中可定量地被石蜡萃取 ,而Bi3+ 一双硫腙显色络合物在NH4 AC -HAC(pH =5)介质中可定量被石蜡萃取 ,两者固相色阶明显。铅、铋均在 0～ 2 0 μg/30mL范围内符合比尔定律 ,从而建立了连续测定铅和铋的固相分光光度法。

关键词：石蜡相分光光度法铅铋双硫腙测定

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应力性骨折战士跟骨骨密度分析

解放军医学院学报 2020年第1期41卷 50-52,69页

作者：赵燕旭卫光星许文静李亚红代永静苏庆军万一群赵斌彭江张民泽唐佩福卢世璧汪爱媛解放军总医院第一医学中心骨科研究所北京100853 延安大学附属医院陕西延安716000 32133部队山西大同037000 中部战区空军医院山西大同037006

目的探讨跟骨骨密度与应力性骨折之间的关系,为应力性骨折的预防提供依据。方法选取2017年12月某部在集训期间发生应力性骨折的23例男性新兵作为观察对象,以该部19例参与相同训练的同年龄阶段、同性别的健康新兵作为对照。两组人员均由... 详细信息

目的探讨跟骨骨密度与应力性骨折之间的关系,为应力性骨折的预防提供依据。方法选取2017年12月某部在集训期间发生应力性骨折的23例男性新兵作为观察对象,以该部19例参与相同训练的同年龄阶段、同性别的健康新兵作为对照。两组人员均由同一名技师使用超声骨密度仪测量跟骨骨密度。结果两组新兵的年龄、身高、体质量等基线水平均无统计学差异。应力性骨折组跟骨密度Z值为(-0.383±0.903) g/cm^2,正常对照组跟骨密度Z值为(0.247±0.897) g/cm^2,差异有统计学意义(P<0.05)。结论我们建议在条件允许的情况下对军事人员及运动员等进行跟骨骨密度检查,做到早筛查、早诊断、早治疗,以便对高风险人群进行健康指导。

关键词：应力性骨折跟骨骨密度新兵

OsVPS9A Functions Cooperatively with OsRAB5A to Regulate Post-Golgi Dense Vesicle-Mediated Storage Protein Trafficking to the Protein Storage Vacuole in Rice Endosperm Cells

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Molecular Plant 2013年第6期6卷 1918-1932页

作者： Feng Liu Yulong Ren Yihua wang Cheng Peng Kunneng Zhou Jia Lv Xiuping Guo Xin Zhang Jianmin wan yiqun Bao State Key Laboratory for Crop Genetics and Germplasm Enhancement Jiangsu Plant Gene Engineering Research Center Nanjing Agricultural UniversityNanjing 210095 China National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Science Chinese Academy of Agricultural Sciences Beijing100081 China College of Life Sciences Nanjing Agricultural University Nanjing 210095 China

In the rice endosperm cells, glutelins are synthesized on rough endoplasmic reticulum as proglutelins and are sorted to the protein storage vacuoles （PSVs） called protein body IIs （PBIIs）, where they are converted to the mature forms. Dense vesicle （DV）-mediated trafficking of proglutelins in rice seeds has been proposed, but the post-Golgi control of this process is largely unknown. Whether DV can fuse directly with PSV is another matter of debate. In this study, we propose a regulatory mechanism underlying DV-mediated, post-Golgi proglutelin trafficking to PBII （PSV）. gpa2, a loss- of-function mutant of OsVPS9A, which encodes a GEF of OsRAB5A, accumulated uncleaved proglutelins. Proglutelins were mis-targeted to the paramural bodies and to the apoplast along the cell wall in the form of DVs, which led to a con- comitant reduction in PBII size. Previously reported gpal, mutated in OsRab5a, has a similar phenotype, while gpalgpa2 double mutant exacerbated the conditions. In addition, OsVPS9A interacted with OsRAB5A in vitro and in vivo. We con- cluded that OsVPS9A and OsRAB5A may work together and play a regulatory role in DV-mediated post-Golgi proglutelin trafficking to PBII （PSV）. The evidence that DVs might fuse directly to PBII （PSV） to deliver cargos is also presented.

关键词： 57H mutant dense vesicle PSV OsVPS9A OsRAB5A.