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Food Science and Human Wellness 2024年第4期13卷 2102-2115页

作者： Xin Hu Dan Cheng yingxia zhang Po Li Xiaoping Wu Junsheng Fu College of Life Sciences Fujian Agriculture and Forestry UniversityFuzhou 350002China Mycological Research Center Fujian Agriculture and Forestry UniversityFuzhou 350002China Shanghai Lithy One-Health Group Technology Co. Ltd.Shanghai 200235China

Autoimmune hepatitis(AIH)is a chronic inflammatory liver disease that threatens human health *** aim of this study was to detect the protective effect of a fermented Lentinus edodes extract containingα-glucan(FLA),in a concanavalin A(Con A)-induced AIH mouse model and to determine the underlying liver-protective *** results showed that compared with the model group,the level of proinflammatory cytokines in serum of FLA pretreated mice was significantly decreased,and the degree of inflammatory cell infiltration in liver,thymus and spleen was significantly *** polymerase chain reaction,immunohistochemistry,and Western blotting showed that FLA pre-treatment inhibited the Con A-induced apoptosis of hepatocytes by down-regulating the expression of BAX and up-regulating the expression of *** research found that FLA may improve liver injury in mice by activating NRF2 signaling pathway and inhibiting TRAF6/NF-κB signaling ***,FLA may improve liver injury in mice by shifting gut microbial composition to reduce the release of inflammatory cytokines in the serum and prevent the necrosis of ***-regulation of NRF2 signaling pathway,down-regulation of TRAF6/NF-κB signaling pathway,and an increase in the relative abundance of Lactobacillus_johnsonii and Ligilactobacillus_murinus play a protective role in liver.

关键词： Autoimmune hepatitis Concanavalin A Lentinus edodes TRAF6/NF-κB NRF2

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不可逆电穿孔技术治疗恶性肿瘤研究进展

中国医学影像技术 2024年第6期40卷 928-931页

作者：武史惠邓伟张英霞内蒙古医科大学附属医院超声诊断科内蒙古呼和浩特010000

不可逆电穿孔(IRE)技术通过施加电场使细胞发生凋亡而不产生热效应、不损伤脉管结构,与化学治疗和/或免疫疗法联合使用时疗效更佳,是治疗恶性肿瘤的新手段。本文就IRE技术原理及其治疗恶性肿瘤研究进展进行综述。

关键词：不可逆电穿孔疗法消融技术

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荧光偏振免疫法测定水产品中的丁香酚

分析试验室 2024年第3期43卷 307-313页

作者：王强王旭峰张英侠黄珂中国水产科学研究院南海水产研究所农业农村部水产品加工重点实验室广州510300

将丁香酚(Eul)与丙烯酸甲酯进行衍生化反应,合成了一种新型的丁香酚半抗原4-(4羟基-3-甲氨基苯基)-丁-2-烯酸(Eul-Aca)。采用活性酯法将丁香酚半抗原与乙二胺异硫氰酸荧光素(EDF)偶联,分别制备了同源和异源荧光示踪物。通过比较不同示... 详细信息

将丁香酚(Eul)与丙烯酸甲酯进行衍生化反应,合成了一种新型的丁香酚半抗原4-(4羟基-3-甲氨基苯基)-丁-2-烯酸(Eul-Aca)。采用活性酯法将丁香酚半抗原与乙二胺异硫氰酸荧光素(EDF)偶联,分别制备了同源和异源荧光示踪物。通过比较不同示踪物的抗体稀释度和灵敏度,优化反应时间等检测条件,建立了一种检测水产品中丁香酚的荧光偏振免疫分析法(FPIA)。结果表明:异源示踪物(Eul-AcaEDF)具有更优的检测灵敏度,抗体稀释度为1/200,反应时间仅需5 min。该方法的半抑制浓度(IC_(50))为11.2μg/L,检测线性范围为1.1~111.5μg/L,检出限(LOD)为0.24μg/L。实际样品中丁香酚的加标回收率为80.6%~107.4%,相对标准偏差(RSD)<15%。FPIA检测结果与气相色谱-质谱(GC-MS)法结果具有良好的一致性(r=0.993),适用于水产品中丁香酚的残留检测。

关键词：荧光偏振免疫分析法示踪物丁香酚水产品

甲状腺微小乳头状癌微波消融术后疗效评估的影响因素分析

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中国中西医结合影像学杂志 2024年第3期22卷 302-305,354页

作者：邓伟张英霞闫诺李昊昌内蒙古医科大学附属医院超声科内蒙古呼和浩特010000

目的:探讨超声引导下微波消融治疗甲状腺微小乳头状癌(PTMC)的临床疗效及结节体积的影响因素。方法:纳入行微波消融治疗的低危PTMC患者63例,于术后1 d和1、3、6、12个月进行随访,对临床疗效及结节体积进行观察;根据结节吸收情况分为结... 详细信息

目的:探讨超声引导下微波消融治疗甲状腺微小乳头状癌(PTMC)的临床疗效及结节体积的影响因素。方法:纳入行微波消融治疗的低危PTMC患者63例,于术后1 d和1、3、6、12个月进行随访,对临床疗效及结节体积进行观察;根据结节吸收情况分为结节消失组(19例)与结节未消失组(43例),分析影响结节体积的相关因素。结果:63例均一次性完全消融成功,1例因颈部淋巴结转移转行外科手术。术后疼痛1例、出血2例、声音改变1例,对症处理后均恢复正常,无其他不良反应及并发症。微波消融后结节体积短时间内急剧增大,术后6个月时结节体积恢复至术前水平,19例结节术后12个月完全吸收,其中4例发生在术后6个月。2组在有无微小钙化灶、与最近被膜的距离、甲状腺影像报告和数据系统(TI-RADS)分类、术后1 d结节体积、消融时间、消融热量及性别方面差异均无统计学意义(均P>0.05);2组年龄、术前结节体积、单位体积能量差异均有统计学意义(均P<0.05)。结论:超声引导下微波消融治疗低危PTMC的效果良好,安全性高。年轻患者、小病灶及单位体积能量高的患者微波消融术后结节吸收效果好。

关键词：微波消融甲状腺微小乳头状癌临床疗效吸收率

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褐变抑制剂对果蔬多酚氧化酶的影响

食品安全导刊 2024年第3期 173-176页

作者：刘文静张映霞罗立新张建良张锐郭众仲新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室新疆阿拉尔843300 南疆特色果树高效优质栽培与深加工技术国家地方联合工程实验室新疆阿拉尔843300 塔里木大学园艺与林学学院新疆阿拉尔843300

果蔬贮藏过程中,褐变问题严重影响其经济价值及营养价值,由多酚氧化酶引起的酶促褐变备受关注,寻找合适的抑制方法是国内外研究的热点。一些化学抑制剂对于酶促褐变具有良好的抑制效果,本文综述了果蔬褐变的机理,抗坏血酸、半胱氨酸、... 详细信息

果蔬贮藏过程中,褐变问题严重影响其经济价值及营养价值,由多酚氧化酶引起的酶促褐变备受关注,寻找合适的抑制方法是国内外研究的热点。一些化学抑制剂对于酶促褐变具有良好的抑制效果,本文综述了果蔬褐变的机理,抗坏血酸、半胱氨酸、柠檬酸抑制剂的作用原理及具体应用,旨在为解决由酶促褐变引起的果蔬生产加工问题提供参考,促进果蔬品质的提升。

关键词：褐变抑制剂多酚氧化酶

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甲基苯丙胺相关肾损害的机制与治疗研究进展

中华肾病研究电子杂志 2024年第3期13卷 166-169页

作者：禹杭远王月琪张英霞张利周艳刘畅李元子北京市利康教育矫治所 102600 中国自然资源经济研究院北京100032 首都医科大学附属北京朝阳医院 100020

甲基苯丙胺是一种高度成瘾性的拟交感神经制剂,主要在肝脏代谢并通过肾脏排出体外。甲基苯丙胺除具有神经毒性作用外,还可导致急性肾功能衰竭和慢性肾功能不全,其机制包括肾血管损伤、高热、电解质紊乱、非创伤性横纹肌溶解和直接肾毒... 详细信息

甲基苯丙胺是一种高度成瘾性的拟交感神经制剂,主要在肝脏代谢并通过肾脏排出体外。甲基苯丙胺除具有神经毒性作用外,还可导致急性肾功能衰竭和慢性肾功能不全,其机制包括肾血管损伤、高热、电解质紊乱、非创伤性横纹肌溶解和直接肾毒性等。甲基苯丙胺相关肾损害的初期症状不明显,待确诊时往往已经出现了肾脏器质性改变。因此,早期评估和干预是降低甲基苯丙胺相关肾损害的关键。本文综述了甲基苯丙胺相关肾损害的作用机制与治疗研究进展。

关键词：甲基苯丙胺急性肾衰竭高热横纹肌溶解

Self-adjuvant Astragalus polysaccharide-based nanovaccines for enhanced tumor immunotherapy:a novel delivery system candidate for tumor vaccines

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Science China(Life Sciences) 2024年第4期67卷 680-697页

作者： Nan Li Yun zhang Miaomiao Han Tian Liu Jinjia Wu yingxia Xiong Yikai Fan Fan Ye Bing Jin Yinghua zhang Guibo Sun Xiaobo Sun Zhengqi Dong State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs Chinese Academy of Medical Sciences&Peking Union Medical College(CAMS)Institute of Medicinal Plant Development(IMPLAD)Beijing 100193China Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine from Ministry of Education Beijing Key Laboratory of Innovative Drug Discovery of Traditional Chinese Medicine(Natural Medicine)and Translational MedicineKey Laboratory of New Drug Discovery Based on Classic Chinese Medicine Prescription from Chinese Academy of Medical SciencesCAMSIMPLADBeijing 100193China Department of Pharmaceutics School of Pharmaceutical SciencesHeilongjiang University of Chinese MedicineHarbin 150036China Jilin Academy of Chinese Medicine Sciences Changchun 130012China Joint Research Center for Chinese Medicinal Herbs IMPLADABRC&ACCLBeijing 100193China

The study of tumor nanovaccines(NVs)has gained interest because they specifically recognize and eliminate tumor ***,the poor recognition and internalization by dendritic cells(DCs)and insufficient immunogenicity restricted the vaccine ***,we extracted two molecular-weight Astragalus polysaccharides(APS,12.19 k D;APSHMw,135.67 k D)from Radix Astragali and made them self-assemble with OVA257–264directly forming OVA/APS integrated nanocomplexes through the microfluidic *** nanocomplexes were wrapped with a sheddable calcium phosphate layer to improve *** in the formed nanocomplexes served as drug carriers and immune adjuvants for potent tumor *** optimal APS-NVs were approximately 160 nm with uniform size distribution and could remain stable in physiological saline *** FITC-OVA in APS-NVs could be effectively taken up by DCs,and APS-NVs could stimulate the maturation of DCs,improving the antigen cross-presentation efficiency in *** possible mechanism was that APS can induce DC activation via multiple receptors such as dectin-1 and Toll-like receptors 2 and *** accumulation of APS-NVs both in draining and distal lymph nodes were observed following *** APS-NVs could easily access the lymph ***,APS-NVs could markedly promote antigen delivery efficiency to DCs and activate cytotoxic T *** addition,APS-NVs achieve a better antitumor effect in established B16-OVA melanoma tumors compared with the OVA+Alum treatment *** antitumor mechanism correlated with the increase in cytotoxic T cells in the tumor ***,the poor tumor inhibitory effect of APS-NVs on the nude mouse model of melanoma also confirmed the participation of antitumor adaptive immune response induced by ***,this study developed a promising APS-based tumor NV that is an efficient tumor immunotherapy without systemic side effects.

关键词： Astragalus polysaccharides nanovaccines OVA immunotherapy self-adjuvant

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Early and Sensitive Detection of Pathogens for Public Health and Biosafety:An Example of Surveillance and Genotyping of SARS-CoV-2 in Sewage Water by Cas12a-Facilitated Portable Plasmonic Biosensor

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Research 2024年第2期2023卷 259-272页

作者： Tianzhong Li Yuzhi Chen Zhi Chen Yuan Hao Minyi Liang yingxia Liu Guanyong Ou Huanian zhang Yuxuan Tang Yabing Hao Swelm Wageh Omar AAl-Hartomy Abul Kalam Bin zhang Xin Shi Xuejin Li Han zhang College of Physics and Optoelectronic Engineering Institute of Translational MedicineDepartment of OtolaryngologyShenzhen Second People's HospitalFirst Affiliated Hospital of Shenzhen UniversityHealth Science CenterShenzhen UniversityShenzhen 518060China Shenzhen Key Laboratory of Sensor Technology Shenzhen 518060China The Sixth Affiliated Hospital of Guangzhou Medical University Qingyuan People's HospitalQingyuan 511518China Shenzhen Key Laboratory of Pathogen and Immunity National Clinical Research Center for Infectious DiseaseState Key Discipline of Infectious DiseaseShenzhen Third People's HospitalSecond Hospital Affiliated to Southern University of Science and TechnologyShenzhen 518112China School of Medicine Southern University of Science and TechnologyShenzhen 518055China School of Physics and Optoelectronic Engineering Shandong University of TechnologyZibo 255049China Department of Physics Faculty of ScienceKing Abdulaziz University Jeddah 21589Saudi Arabia Research Center for Advanced Materials Science(RCAMS) King Khalid UniversityP.0.Box 9004Abha 61413Saudi Arabia Department of Chemistry College of ScienceKing Khalid UniversityP.0.Box9004Abha 61413 Saudi Arabia Health Sciences Institute China Medical UniversityShenyang 110000China The Chinese University of Hong Kong Shenzhen 518060China

Infectious diseases severely threaten public health and global *** addition to transmission through the air,pathogenic microorganisms have also been detected in environmental liquid samples,such as sewage *** biochemical detection methodologies are time-consuming and cost-ineffective,and their detection limits hinder early *** the present study,ultrafine plasmonic fiber probes with a diameter of 125μm are fabricated for clustered regularly interspaced short palindromic repeats/CRISPR-associated protein(CRISPR/Cas)-12a-mediated sensing of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).Single-stranded DNA exposed on the fiber surface is trans-cleaved by the Cas12a enzyme to release gold nanoparticles that are immobilized onto the fiber surface,causing a sharp reduction in the surface plasmon resonance(SPR)*** proposed fiber probe is virus-specific with the limit of detection of~2,300 copies/ml,and genomic copy numbers can be reflected as shifts in wavelengths.A total of 21 sewage water samples have been examined,and the data obtained are consistent with those of quantitative polymerase chain reaction(qPCR).In addition,the Omicron variant and its mutation sites have been fast detected using S gene-specific *** study provides an accurate and convenient approach for the real-time surveillance of microbial contamination in sewage water.

关键词： fiber consuming hinder

Ethylene Oligomerization and Polymerization: Alternative Iron Catalysts beyond 2,6-Bisiminopyridyl Iron Complexes

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复旦学报（自然科学版） 2005年第5期44卷 838-839页

作者： Suyun Jie Shu zhang Wenjuan zhang yingxia Song Junxian Hou Wen-Hua Sun Key Laboratory of Engineering Plastics Institute of Chemistry Chinese Academy of Sciences Beijing 100080 China

Polyolefin industry arrives the option to transfer from multiple-site systems of the classical Ziegler-Natta catalysts to more sophisticated single-site catalysts. The late-transition metal compounds were traditionally assumed with poor polymerization properties due to the highly competitive chain-termination step, and produced the short-chain oligomers up to 40 carbon atoms (SHOP catalysts). Recently the polyolefins employing latetransition metal complexes as catalysts became a hot research subject with the pioneering works by Brookhart and Gibson. It is promising for nickel catalysts to use solely ethylene as monomer for highly branched polyethylenes, and the designed nickel catalysts were not useful in industry. It is critical time to investigate the relationship of coordination modes of nickel complexes and its catalytic activities and the properties of resultant polyethylenes.

关键词：聚乙烯低聚反应催化剂铁化合物聚合物

小学生速度概念学习进阶:模型构建与水平分析

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天津师范大学学报（基础教育版） 2023年第5期24卷 19-25页

作者：张英霞丁锐东北师范大学教育学部吉林长春130024

速度概念是小学中重要且较难的跨学科概念。围绕着速度这一核心概念,主要采用访谈法、专家咨询法构建了小学生速度概念学习进阶初始模型。使用测验法和Rasch模型对该进阶模型的有效性进行检验和修订,并对学生的表现进行了分析。结果表... 详细信息

速度概念是小学中重要且较难的跨学科概念。围绕着速度这一核心概念,主要采用访谈法、专家咨询法构建了小学生速度概念学习进阶初始模型。使用测验法和Rasch模型对该进阶模型的有效性进行检验和修订,并对学生的表现进行了分析。结果表明研究工具的质量良好,学生的实际表现与前期构建的速度概念进阶模型基本相符;最终得到的小学生速度概念学习进阶模型包括五个层级(具备速度的经验、辨别速度的形式、认识速度的本质、理解速度的本质、应用数量关系)九个水平;不同年级学生的速度概念发展水平有显著差异。构建的小学生速度概念学习进阶模型,可以作为跨学科教材编写的参考依据,也为指向大概念的深度教学提供了证据支持。

关键词：速度概念学习进阶 Rasch模型