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Influence of lactic acid bacteria, cellulase, cellulase-producing Bacillus pumilus and their combinations on alfalfa silage quality

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Journal of Integrative Agriculture 2018年第12期17卷 2768-2782页

作者： LI Dong-xia NI Kui-kui ZHANG ying-chao lin Yan-li YANG Fu-yu College of Animal Science and Technology China Agricultural UniversityBeijing 100193P.R.China Beijing Sure Academy of Biosciences Beijing 100085P.R.China

This study assessed the effects of lactic acid bacteria(LAB), cellulase, cellulase-producing Bacillus pumilus and their combinations on the fermentation characteristics, chemical composition, bacterial community and in vitro digestibility of alfalfa silage. A completely randomized design involving a 8(silage additives)×3 or 2(silage days) factorial arrangement of treatments was adopted in the present study. The 8 silage additive treatments were: untreated alfalfa(control); two commercial additives(GFJ and Chikuso-1); an originally selected LAB(Lactobacillus plantarum a214) isolated from alfalfa silage; a cellulase-producing Bacillus(CB) isolated from fresh alfalfa; cellulase(C); and the combined additives(a214+C and a214+CB). Silage fermentation characteristics, chemical composition and microorganism populations were analysed after 30, 60 and 65 days(60 days followed by exposure to air for five additional days). In vitro digestibility was analysed for 30 and 60 days. Compared with the other treatments, selected LAB a214, a214 combined with either C or CB, and Chikuso-1 had the decreased(Pling process, and there were no differences(P>0.05) among them. Fiber degradation was not significant(P≥0.054) in any C or CB treatments. The a214 treatment showed the highest(P=0.009) in vitro digestibility of dry matter(595.0 g kg–1DM) after ensiling and the highest abundance of Lactobacillus(69.42 and 79.81%, respectively) on days 60 and 65, compared to all of other treatments. Overall, the silage quality of alfalfa was improved with the addition of a214, which indicates its potential as an alfalfa silage inoculant.

关键词： alfalfa silage cellulase fermentation quality invitro digestibility lactic acid bacteria

体外诱导中性粒细胞胞外诱捕网形成条件的优选及验证

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中国中药杂志 2024年第9期49卷 2336-2344页

作者：戚明珠李卓航黄荷兰祝盼盼朱鏐娈林娜苏晓慧孔祥英中国中医科学院中药研究所北京100700 首都医科大学附属北京地坛医院北京100015

旨在优选体外诱导中性粒细胞胞外诱捕网(neutrophil extracellular traps,NETs)形成的条件,以建立相对稳定的实验技术平台。通过比较不同条件,包括大鼠和小鼠等常用的实验动物,利用percoll密度梯度离心法分离骨髓中性粒细胞、外周血中... 详细信息

旨在优选体外诱导中性粒细胞胞外诱捕网(neutrophil extracellular traps,NETs)形成的条件,以建立相对稳定的实验技术平台。通过比较不同条件,包括大鼠和小鼠等常用的实验动物,利用percoll密度梯度离心法分离骨髓中性粒细胞、外周血中性粒细胞等多种细胞来源,采用脂多糖(lipopolysaccharide,LPS)和佛波醇12-肉豆蔻酸酯13-乙酸酯(phorbol 12-myristate 13-acetate,PMA)不同诱导剂体外诱导,通过MPO/CitH3/DAPI免疫荧光标记法以及细胞培养上清游离DNA(cell free DNA,cfDNA)含量检测综合评价,筛选体外诱导NETs形成的优选条件;在上述平台选择基础上,进一步通过给予中药有效单体川芎嗪(tetramethylpyrazine,TMP)进行验证,综合评估优选的体外NETs诱导条件的稳定性。结果显示,包被多聚赖氨酸可一定程度促进小鼠骨髓中性粒细胞NETs形成。LPS和PMA均可显著促进小鼠骨髓中性粒细胞MPO、CitH3蛋白表达,并提高大鼠外周血中性粒细胞培养上清中cfDNA含量;且与LPS相比,PMA诱导cfDNA水平升高更显著(P<0.05)。与PMA共孵育后小鼠骨髓中性粒细胞、大鼠骨髓中性粒细胞和大鼠外周血中性粒细胞中MPO、CitH3蛋白表达均显著升高,以大鼠外周血中性粒细胞升高最明显。TMP可以显著下调PMA诱导的大鼠外周血中性粒细胞中MPO、CitH3和NE蛋白的表达。综上,PMA诱导大鼠外周血中性粒细胞是体外诱导NETs形成的较优条件。该研究为基于NETs的体外研究提供优选平台,并为以调控NETs为靶标的中药作用研究提供前期基础。

关键词：中性粒细胞胞外诱捕网免疫荧光骨髓外周血川芎嗪

口服与静脉注射氨甲环酸在原发性全髋关节和膝关节置换术中的有效性和安全性分析

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四川生理科学杂志 2024年第9期46卷 1916-1919页

作者：刘文坤赵颖王允江邢林卿商丘市第三人民医院骨科河南商丘476000

目的:探究氨甲环酸采用口服和静脉注射的给药方式在原发性全髋关节和膝关节置换术中的有效性和安全性。方法:选取我院2022年11月至2023年11月收治的136例原发性全髋关节和膝关节置换术患者,随机分为对照组、研究组,各68例,对照组给予静... 详细信息

目的:探究氨甲环酸采用口服和静脉注射的给药方式在原发性全髋关节和膝关节置换术中的有效性和安全性。方法:选取我院2022年11月至2023年11月收治的136例原发性全髋关节和膝关节置换术患者,随机分为对照组、研究组,各68例,对照组给予静脉注射氨甲环酸1.0 g治疗,研究组给予口服氨甲环酸2.0 g治疗。观察记录两组治疗前、治疗后3 d出血量及治疗前、治疗后3 d应用全自动凝血分析仪、酶联免疫吸附法测定凝血功能、炎症因子及血管内皮功能指标水平,分析临床效果。结果:治疗后,相比对照组,研究组出血量、凝血原酶时间(Prothrombin time,PT)、活化部分凝血活酶时间(Activated partial thromboplastin time,APTT)水平、可溶性血栓调节蛋白(Soluble thrombmodulin,sTM)、E-选择素、血管内皮生长因子(Vascular endothelial growth factor,VEGF)水平略低(P>0.05);纤维蛋白原(Fibrinogen,FIB)水平、C反应蛋白(C-reactive protein,CRP)、白细胞介素-6(Interleukin-6,IL-6)水平及治疗总有效率略高(P>0.05)。研究组并发症发生率低于对照组,差异有统计学意义(P<0.05)。结论:口服与静脉注射氨甲环酸用于原发性全髋关节和膝关节置换术中均有显著治疗效果,但口服氨甲环酸安全性更高,值得推广。

关键词：口服静脉注射氨甲环酸原发性全髋关节膝关节置换术

Efficacy of Pudilan Xiaoyan Oral Liquid in the treatment of pneumonia induced by drug-resistant Pseudomonas aeruginosa

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Infectious Diseases Research 2024年第3期5卷 22-29页

作者： Jing Ma Yan-Yan Bao Xiao-Rui Wang Kuok Kuantai Leong-Man Chon Xiao-Lan Cui Ju-ying Zhong Yu-Jing Shi Institute of Chinese Materia Medica China Academy of Chinese Medical SciencesBeijing 100700China Conde S.Januário Hospital(CHCSJ) pharmacy departmentMacao 999078China lin Fong Charities Traditional Chinese Medicine Treatment Centre Macao 999078China Experimental Research Center China Academy of Chinese Medical SciencesBeijing 100700China

Background:Pudilan Xiaoyan Oral Liquid(PDL)is a Chinese patent medicine with notable pharmacological properties,including anti-inflammatory and antibacterial ***-resistant Pseudomonas aeruginosa infection is a common and refractory bacterial infection in clinical *** to its high drug resistance,it brings great challenges to *** study aimed to assess the therapeutic efficacy of PDL in a murine model of pneumonia induced by drug-resistant Pseudomonas ***:Three different doses of PDL(11 mL/kg/d,5.5 mL/kg/d,2.75 mL/kg/d)were used to observe lung tissue pathology and inflammatory cytokine levels in pneumonia mouse models induced by multidrug-resistant Pseudomonas aeruginosa(MDR-PA).Additionally,the protective efficacy of PDL against mortality in infected mice was evaluated using a death model caused by *** sub-MIC concentration of levofloxacin was used to induce drug-resistant mice pneumonia model to evaluate the role of PDL in reversing drug *** data are expressed as mean±standard *** significance was determined by one-way analysis of variance followed by Tukey’s multiple-comparisons ***:Treatment effect of PDL on MDR-PA pneumonia:the medium and small doses of PDL can significantly reduce the lung index of multi-drug resistant bacteria infected pneumonia model mice(P<0.05),the lung index inhibition rates for these groups were 55.09%and 58.43%,and improve the degree of lung tissue lesions of mice;The expression of serum cytokines keratinocyte chemoattractant,tumor necrosis factor-αand monocyte chemoattractant protein-1 could be decreased in the three dosage groups of PDL(P<0.01).PDL treatment not only lowered the mortality but also extended the survival duration in mice infected with *** was found after sub-MIC concentration of levofloxacin induced resistance of Pseudomonas aeruginosa to pneumonia in *** with the model group,the lung index of mice in high

关键词： pudilan Pseudomonas aeruginosa drug resistance mice model

DIAGNOSIS AND TREATMENT OF MEDIASTINAL ENTEROGENOUS CYSTS IN CHILDREN

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Chinese Medical Sciences Journal 2006年第3期21卷 201-203页

作者： Ke-ren Zhang Hui-min jia En-yuan Pan Lian-ying Wang Department of Pediatric Surgery the Second Clinical College China Medical University Shenyang 110004

Objective To investigate the diagnosis and therapy of mediastinal enterogenous cysts in children. Methods Clinical data of 17 cases with mediastinal enterogenous cysts within 19 years in our hospital were retrospectively analyzed. Results One case was intramural esophageal cyst and 16 cases were enteric cysts, two among which were complicated with abdominal enteric duplications. Most cases presented with symptoms of respiratory distress. Twelve cases were complicated with vertebral anomalies. Ultrasound helpful in confirming the cystic nature of these lesions. posterior mediastinum. of 12 cases and magnetic resonance imaging of 4 cases were Eight cases had technetium-99m pertechnetate scintigraphy of posterior mediastinum.Conclusions Most patients present with symptoms of respiratory distress, complicated with vertebral anomalies. Ultrasonography and magnetic resonance imaging may be helpful in confirming the cystic nature of these lesions. Technetium-99m pertechnetate scintigraphy is the most effective method for differentiation of the disease from other mediastinal cysts.

关键词： mediastinal mass enteric cysts diagnosis therapy

变电站多元融合全站域智能防误系统设计与实现

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自动化技术与应用 2024年第9期43卷 163-166,188页

作者：董仲星林昊郭雄白何颖梅国网杭州供电公司浙江杭州310051

针对变电站防误系统仅考虑一次设备防误的管控策略难以控制变电站现场作业风险的问题,提出适应“运检一体化”模式的变电站多元融合全站域智能防误系统设计方案。该系统在原有微机防误技术的基础上,通过电子模拟图板软件整合站内各部分... 详细信息

针对变电站防误系统仅考虑一次设备防误的管控策略难以控制变电站现场作业风险的问题,提出适应“运检一体化”模式的变电站多元融合全站域智能防误系统设计方案。该系统在原有微机防误技术的基础上,通过电子模拟图板软件整合站内各部分数据孤岛,实现了变电站锁控、一二次设备统筹防误以及安全工器具管理的全站域安全管控;将防误理念拓展到二次设备操作防误、检修作业防误和运维作业防误;完成了各类操作的可视见、可干预、可追溯,能够控制变电站现场作业风险,实现了全站域的业务共享和数据融合。

关键词：多元融合智能防误二次设备防误域安全管控系统设计

Dynamic Mechanical Behavior and Prediction for PP/POE Blends

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Chemical Research in Chinese Universities 2009年第4期25卷 573-578页

作者： ying Ji-ru XIE Xiao-lin ZHOU Xing-ping ZHOU Hua-min LI De-qun State Key Laboratory of Plastic Forming Simulation and Die ＆ Mould Technology Wuhan 430074 P. R. China Hubei Key Laboratory of Materials Chemistry and Service Failure Department of Chemistry and Chemical Engineering Huazhong University of Science and Technology Wuhan 430074 P. R. China School of Chemical and Environmental Engineering Hubei University of Technology Wuhan 430068 P. R. China

Polypropylene（PP）/ethylene-octene copolymer（POE） blends were prepared with a twin-screw *** dynamic mechanical behavior were systematically *** results show that PP/POE blends are heterogeneities with a partial compatible two-phase structure,the glass transition temperature of PP phases in the blends tends to shift towards high temperature with increasing the POE content,and the glass transition temperature of POE phases shifts towards the low temperature with increasing the PP *** Kerner＇s dispersed phase model and co-continuous phase model can reasonably predict the visco-elasticity of PP/POE blends with different ***,the morphological structure of the blends can be estimated via comparing the predicted DMA behavior with the experimental data.

关键词： PP/POE blend Dynamic mechanical behavior Dispersed phase model Co-continuous phase model

永定河干流雁翅站“23·7”流域性特大洪水特征分析

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水科学与工程技术 2024年第4期 1-3页

作者：薛栋元连玉柱唐磊贾祯晖林宬北京市水文总站北京100089

明确极端暴雨下永定河洪水流量特征,为应对极端暴雨提供技术支撑。基于2305号台风“杜苏芮”导致的“23·7”流域性特大洪水,根据永定河干流雁翅水文站洪水过程中连续监测水文数据,分析了此次暴雨中永定河水系的降水及洪水过程特征。结... 详细信息

明确极端暴雨下永定河洪水流量特征,为应对极端暴雨提供技术支撑。基于2305号台风“杜苏芮”导致的“23·7”流域性特大洪水,根据永定河干流雁翅水文站洪水过程中连续监测水文数据,分析了此次暴雨中永定河水系的降水及洪水过程特征。结果表明:2023年7月29日—8月6日,雁翅站洪水径流总量为2.03亿m^(3),实测洪峰流量为1710 m^(3)/s,为1951年建站以来的最大流量,洪峰重现期超过20年一遇。该研究为永定河水旱灾害防御工作提供借鉴参考。

关键词：永定河 “23·7”流域性特大洪水台风洪水特性

Screening the anti-gout traditional herbs from TCM using an in vitro method

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Chinese Chemical Letters 2016年第11期27卷 1701-1707页

作者： Wei-jia Chen Yi Wu Xin Zhao Shu Liu Feng-Rui Song Zhong-ying Liu Zhi-Qiang Liu School of Pharmaceutical Sciences Jilin University National Center of Mass Spectrometry in Changchun & Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry Changchun Institute of Applied ChemistryChinese Academy of Sciences

The aim of this work was to evaluate the ability of 33 herbal extracts in inhibiting the acute inflammation and xanthine oxidase （XOD） activity. The anti-inflammation effects of the herbal extracts were detected by an in vitro cell model, which was established by stimulating human umbilical vein endothelial cells （HUVEC） using sodium urate （MSU）. In this model, the intercellular adhesion molecule-1 （ICAM-1） and interleukin-1 beta （IL-1β） were expressed, and the anti-inflammation effects of herbal extracts were evaluated by detecting the content changes of ICAM-1 and IL-1β in cell lysates and cell culture supernates using an enzyme-linked immunosorbent assay （ELISA）. Moreover, an ultrahigh performance liquid chromatography and tandem mass spectrometry （UPLC-MS/MS） method was used for the detection of XOD activity and the screening of XOD inhibitors in this research. The amount of uric acid from each analyte was directly detected using the multiple reaction monitoring mode and the uric acid level could be reduced via the addition of an inhibitor. Results indicated that Salviae Miltiorrhizae Radix et Rhizome, Rhei Radix et Rhizoma, Polygoni Cuspidati Rhizoma et Radix, Selaginellae Herba, Paeoniae Radix Rubra, especially Ginkgo Folium seemed to be more effective in anti-inflammation and inhibiting XOD activity. The anti-inflammation and enzyme inhibitory activities of the herbal extracts may be correlated with their bioactive components. And the differences between the herbal extracts were correlated with the amount of flavonoid and anthraquinone components. In our study, we have investigated the potential anti-inflammation bioactivity of 33 herbal extracts in vitro, which could provide a reference for further in vivo research in the orevention and treatment of gout.

关键词： Gout Inflammation Human umbilical vein endothelial cells Xanthine oxidase UPLC-MS/MS