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Toxic activity of a protein complex purified from Xenorhabdus nematophila HB310 to Plutella xylostella larvae

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Insect Science 2012年第3期19卷 329-336页

作者： Qin-ying Wang Zi-Yan Nangong Jun Yang Ping Song Yi Wang Liwang cui Long cui College of Plant Protection Agricultural University of HebeiBaodingHebei ProvinceChina Department of Entomology Rutgers UniversityNew BrunswickN J Department of Entomology the Pennsylvania State UniversityUniversity ParkPAUSA

Xenorhabdus nematophila, a Gram-negative proteobacterium belonging to the family Enterobacteriaceae and associated symbiotically with soil entomopathogenic nematodes, Steinernema carpocapsae, is pathogenic to a wide range of insects. A protein complex with insecticidal activity was isolated from the cells ofX. nematophila HB310 strain using methods of salting out and native polyacrylamide gel electrophoresis （PAGE）. Seven polypeptides ranging 50~250 kDa were well separated from the protein complex （named Xnpt） by sodium dodecyl sulfate （SDS）-PAGE, five of which are identified as XptA2, xptC 1, XptB 1, GroEL and hypothetical protein by matrix-assisted laser desorption- time-of-flight mass spectrometry （MALDI-TOFMS）. Xnpt showed high oral virulence to larvae of diamondback moth （DBM）, Plutella xylostella L. （Lepidoptera, Plutellidae） as its median lethal concentration （LC50） against second and third instar larvae were 331.45 ng/mL and 553.59 ng/mL at 72 h, respectively. The histological analysis of Xnptfed DBM larvae showed extensive histopathological effects on the midgut. Biochemical analysis indicated that Xnpt markedly inhibited the activities of three important enzymes in the midgut. Overall, our data showed that the protein complex isolated from X. nematophila HB310 induced the antifeedant and death of insects by destroying midgut tissues and inhibiting midgut proteases activities.

关键词： insecticidal toxin midgut Plutella xylostella proteases Xenorhabdus nematophila

Precipitation Kinetics of Cr_2N in High Nitrogen Austenitic Stainless Steel

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Journal of Iron and Steel Research International 2008年第6期15卷 72-77页

作者： SHI Feng WANG Li-jun cui Wen-fang LIU chun-ming School of Materials and Metallurgy Northeastern University Shenyang 110004 Liaoning China

The precipitation behavior of Cr2 N during isothermal aging in the temperature range from 700 ℃ to 950 ℃ in Fe-18Cr-12Mn-0.48N （in mass percent） high nitrogen austenitic stainless steel, including morphology and content of precipitate, was investigated using optical microscopy, scanning electron microscopy, and transmission electron microscopy. The isothermal precipitation kinetics curve of Cr2 N and the corresponding precipitation activation energy were obtained. The results show that Cr2N phase precipitates in a cellular way and its morphology is transformed from initial granular precipitates to lamellar ones in the cell with increasing aging time. The nose temperature of Cr2 N precipitation is about 800 ℃, with a corresponding incubation period of 30 min, and the ceiling temperature of Cr2N precipitation is 950℃. The diffusionactivation energy of Cr2 N precipitation is 296 kJ/mol.

关键词： high nitrogen austenitic stainless steel isothermal aging precipitation kinetics precipitation activation energy

Study of Growth Mechanism of Lysozyme Crystal by Batch Crystallization Method

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Chinese Chemical Letters 2006年第1期17卷 101-104页

作者： Hai Liang cui Yong YU Wan chun CHEN Qi KANG National Microgravity Lab Institute of Mechanics Chinese Academy of Science Beijing 100080 Institute of Physics Chinese Academy of Science Beijing 100080

The lysozyme crystals were made by batch crystallization method and the distribution of aggregate in solution were measured by dynamic light scattering. The results showed that the dimension of aggregate increased with the increase of the concentration of lysozyme and NaCl, lysozyme molecules aggregated gradually in solution and finally arrived at balance each other. The higher the concentrations of lysozyme and NaCl were, the faster the growth rate of （110） face was. The growth rates of lysozyme crystal were obtained by a Zeiss microscope, and the effective surface energy （a） of growing steps were calculated about 4.01×10^-8s J·cm^-2 according to the model of multiple two-dimensional nucleation mechanism.

关键词： Protein crystal growth rate dynamic light scattering lysozymc.

Fiber-like micelle with a p-conjugated polymer core: a potential building block for organic electronics

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Science Bulletin 2017年第18期62卷 1229-1230页

作者： Xian Yang Yinan cui chun Feng Daliao Tao Xiaoyu Huang Key Laboratory of Synthetic and Self-Assembly Chemistry for Organic Functional Molecules Shanghai Institute of Organic Chemistry Chinese Academy of Sciences Shanghai 200032 China

The self-assembly of block copolymer in solution are constantly expanding,in part because of the increasing degree of control over the size,morphology and functionality of self-assembled nanostructures that can be exerted by the introduction of additional driven force such as hydrogen-bonding,halogen bonding,p-p stacking,host-guest complexation,metal-coordination,electrostatic interaction and crystalline[1].Among these,the

关键词：纳米胶束共轭聚合物电子学纤维状主客体相互作用嵌段共聚物自组装控制程度

Effect of Processing on Taste Quality and Health-Relevant Functionality of Sweet Potato Tips

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Agricultural Sciences in China 2011年第3期10卷 456-462页

作者： cui Li LIU chun-quan LI Da-jing SONG Jiang-feng Institute of Farm Product Processing Jiangsu Academy of Agricultural Sciences Nanjing 210014 P.R.China Engineering Research Center for Agricultural Products Processing National Agricultural Science and Technology Innovation Center inEast China Nanjing 210014 P.R.China

The effects of fermenting, boiling, and steaming processes on the levels of free amino acids, organic acids, antioxidant properties （FRAP and DPPH）, phenolic compounds, and angiotensine converting enzyme （ACE） inhibition of sweet potato tips were investigated. Seventeen free amino acids were found in the tips. Fermenting, boiling, and steaming processes significantly reduced total free amino acid contents and steaming processes caused smaller losses than fermenting and boiling processes. Five organic acids were investigated in the tips. In comparison with the total organic acid content of unprocessed sweet potato tips, steaming and boiling processes significantly reduced it, while fermenting process increased it. Chlorogenic acid, rutin, and quercetin were investigated in the tips. The total phenolic compounds contents of sweet potato tips significantly increased by steaming and boiling processes, but a substantial loss was caused by fermenting process. A similar result was shown in FRAP value. The result of ACE inhibitory activity indicated that boiling, steaming, and fermenting processes significantly increased ACE inhibitory activity.

关键词： sweet potato tips amino acids phenolic compounds organic acids ACE antioxidant capacities

Hydrogen production via catalytic decomposition of NH3 using promoted MgO-supported ruthenium catalysts

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Science China Chemistry 2019年第12期62卷 1625-1633页

作者： Xiu-cui Hu Wei-Wei Wang Rui Si Chao Ma chun-Jiang Jia Key Laboratory for Colloid and Interface Chemistry Key Laboratory of Special Aggregated MaterialsSchool of Chemistry and Chemical EngineeringShandong UniversityJinan 250100China Shanghai Synchrotron Radiation Facility Shanghai Institute of Applied PhysicsChinese Academy of SciencesShanghai 201204China College of Materials Science and Engineering Hunan UniversityChangsha 410082China

Catalytic decomposition of NH3 to high purity hydrogen offers a promising strategy for fuel cells, but presents challenges for high hydrogen yields at comparatively low temperatures due to the lack of efficient catalysts. Here, we report the facile preparation of ultra-fine ruthenium（Ru） species dispersed on Mg O, which show excellent activity and high temperature stability for NH3 decomposition reaction. The hydrogen yield of the prepared Ru/Mg O catalysts reaches ca. 2,092 mmol H2 g–1 Ru min–1 at450 °C, far exceeding that of the previously reported most reactive Ru-based catalysts and the same chemical composition samples prepared by other approaches. Various characterization techniques containing X-ray absorption fine structure（XAFS）,in-situ diffuse reflectance infrared Fourier transform spectroscopy（in-situ DRTFTS） and temperature-programmed reduction/desorption（TPR/TPD） were carried out to explore the structure-function relation of the prepared Ru/Mg O catalysts. We found that the Ru species interact strongly with the Mg O support, which can efficiently protect the Ru species and Mg O support from agglomerating during NH3 decomposition test, maintaining the stability of the catalysts.

关键词： Ru catalyst MgO support ammonia decomposition hydrogen production structure-activity relation

Preliminary study on dietary selection in Shortridge’s langurs(Trachypithecus shortridgei)from China

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Zoological Research 2020年第6期41卷 715-720页

作者： ying Geng Jia-Fei He ying-chun Li Zhen-Hua Guan Xiao-Yang He Jun Sun Zhi-Pang Huang Yan-Peng Li Fan Yong Wen Xiao Liang-Wei cui Key Laboratory for Conserving Wildlife with Small Populations in Yunnan Southwest Forestry UniversityKunmingYunnan 650224China Provincial Forestry and Grassland Bureau in Yunnan KunmingYunnan 650224China Nujiang Administration Bureau Gaoligongshan National Nature ReserveLiukuYunnan 673100China Institute of Eastern-Himalaya Biodiversity Research Dali UniversityDaliYunnan 671003China Collaborative Innovation Center for Biodiversity and Conservation in Three Parallel Rivers Region of China DaliYunnan 671003China Provincial Innovation Team of Biodiversity Conservation and Utility of the Three Parallel Rivers Region from Dali University DaliYunnan 671003China Nanjing Institution of Environmental Sciences Ministry of Ecology and Environmental of ChinaNanjingJiangsu 210042China

DEAR EDITOR,Understanding dietary selection and feeding strategies is important for the conservation and management of endangered primate ***,we conducted a preliminary study on the diet and feeding behavior of endangered Shortridge’s langurs(Trachypithecus shortridgei)within the Drung River Valley(Dulongjiang)in southwestern China.

关键词： southwestern Short dietary

Optical wavelet-fractional squeezing combinatorial transform

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Chinese Physics B 2022年第2期31卷 209-216页

作者： cui-Hong Lv ying Cai Nan Jin Nan Huang School of Physics and Electronic Engineering Jiangsu UniversityZhenjiang 212013China

By virtue of the method of integration within ordered product(IWOP)of operators we find the normally ordered form of the optical wavelet-fractional squeezing combinatorial transform(WFrST)*** way we successfully combine them to realize the integration transform kernel of WFr ST is making full use of the completeness relation of Dirac’s ket–bra *** WFr ST can play role in analyzing and recognizing quantum states,for instance,we apply this new transform to identify the vacuum state,the single-particle state,and their superposition state.

关键词： wavelet transform fractional squeezing transform combinatorial transform IWOP technique

Geometrical Structures and Activities of Free Radical Scavenging Studies of Baicalein and Scutellarein

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Chinese Journal of Structural Chemistry 2020年第1期39卷 57-65,2页

作者： QIU Song-Shan JIANG cui-cui ZHOU Ru-Jin LI chun-Hai Development Centre of Technology for Fruit&Vegetable Storage and Processing Engineering School of Biology and Food EngineeringGuangdong University of Petrochemical TechnologyMaoming 525000China

In order to study the free radical scavenging capacity and molecular structures of baicalein and scutellarein,the structure-activity relationship of these two molecules was *** geometric structures of the compounds were optimized by DMol3 code based on density functional *** theoretical parameters of the atomic charge distribution,the distribution of molecular frontier orbital,the energy difference and Fukui functions were ***,superoxide anion free radical and 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)methods were used to determine the scavenging capacity of the *** results showed that the scavenging rate of DPPH radicals and superoxide anion radicals increased with the increase of sample concentration,and had a dose-effect *** radical-scavenging activities in the order of baicalein>scutellarein>BHT were *** p-π conjugation of the hydroxyl oxygen atom influenced the certain ability to repelling electrons in the molecular structures of baicalein and scutellarein based on the data of the atomic charge distribution,the distribution of molecular frontier orbital,the energy difference and Fukui *** groups were more susceptible to attack and exhibit free radical scavenging *** oxygen atom in the phenolic hydroxyl group of the molecule structure of the samples may be the reaction active sites where the electrophilic reaction *** results offer useful theoretical bases for the research and application of antioxidant activities of substances containing such molecular structures.

关键词： density functional theory baicalein scutellarein free radical scavenging