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New influence factor inducing difficulty in selective flotation separation of Cu–Zn mixed sulfide minerals

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International Journal of Minerals,Metallurgy and Materials 2015年第2期22卷 111-115页

作者： Jiu-shuai Deng ying-bo mao Shu-ming Wen Jian Liu Yong-jun Xian Qi-cheng Feng State Key Laboratory of Complex Nonferrous Metal Resources Clean Utilization Faculty of Land Resource Engineering Kunming University of Science and Technology

Selective flotation separation of Cu-Zn mixed sulfides has been proven to be difficult. Thus far, researchers have found no satis- factory way to separate Cu-Zn mixed sulfides by selective flotation, mainly because of the complex surface and interface interaction mecha- nisms in the flotation solution. Undesired activation occurs between copper ions and the sphalerite surfaces. In addition to recycled water and mineral dissolution, ancient fluids in the minerals are observed to be a new source of metal ions. In this study, significant amounts of ancient fluids were found to exist in Cu-Zn sulfide and gangue minerals, mostly as gas-liquid fluid inclusions. The concentration of copper ions re- leased from the ancient fluids reached 1.02 × 10-6 tool/L, whereas, in the cases of sphalerite and quartz, this concentration was 0.62 ×10-6 mol/L and 0.44 × 10-6 mol/L, respectively. As a result, the ancient fluid is a significant source of copper ions compared to mineral dissolution under the same experimental conditions, which promotes the unwanted activation of sphalerite. Therefore, the ancient fluid is considered to be a new factor that affects the selective flotation separation of Cu-Zn mixed sulfide ores.

关键词： sulfide minerals flotation separation fluid inclusions

Transcriptional Regulation of Plant Secondary Metabolism

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Journal of Integrative Plant Biology 2012年第10期54卷 703-712页

作者： Chang-Qing Yang Xin Fang Xiu-Ming Wu ying-bo mao Ling-Jian Wang Xiao-Ya Chen National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for BiologicalSciences the Chinese Academy of Sciences Shanghai 200032 China plant Science Research Center. Shanahai Chensh-an botanical Garden. Shanahai 201602. China

Plant secondary metabolites play critical roles in plant-environment interactions. They are synthesized in different organs or tissues at particular developmental stages, and in response to various environmental stimuli, both biotic and abiotic. Accordingly, corresponding genes are regulated at the transcriptional level by multiple transcription factors. Several families of transcription factors have been identified to participate in controlling the biosynthesis and accumulation of secondary metabolites. These regulators integrate internal （often developmental） and external signals, bind to corresponding cis-elements -- which are often in the promoter regions -- to activate or repress the expression of enzyme-coding genes, and some of them interact with other transcription factors to form a complex. In this review, we summarize recent research in these areas, with an emphasis on newly-identified transcription factors and their functions in metabolism regulation.

关键词： Alkaloid phenylpropanoid secondary metabolism terpenoid transcription factor.

Plant Terpenoids： Biosynthesis and Ecological Functions

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Journal of Integrative Plant Biology 2007年第2期49卷 179-186页

作者： Ai-Xia Cheng Yong-Gen Lou ying-bo mao Shan Lu Ling-Jian Wang Xiao-Ya Chen National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200032 China Institute of Insect Sciences Zhejiang University Hangzhou 310029 China

Among plant secondary metabolites terpenolds are a structurally most diverse group; they function as phytoalexins In plant direct defense, or as signals In Indirect defense responses which involves herbivores and their natural enemies. In recent years, more and more attention has been paid to the Investigation of the ecological role of plant terpenolds. The biosynthesis pathways of monoterpenes, sesquiterpenes, and diterpenes Include the synthesis of C5 precursor isopentenyl diphosphate （IPP） and Its allylic isomer dlmethylallyl dlphosphate （DMAPP）, the synthesis of the immediate diphosphate precursors, and the formation of the diverse terpenoids. Terpene synthases （TPSs） play a key role In volatile terpene synthesis. By expression of the TPS genes, significant achievements have been made on metabolic engineering to Increase terpenoid production. This review mainly summarizes the recent research progress In elucidating the ecological role of terpenoids and characterization of the enzymes Involved in the terpenold biosynthesis. Spatial and temporal regulations of terpenoids metabolism are also discussed.

关键词： chemical ecology metabolism regulation plant terpenoids terpene synthase.

Arabidopsis Transcription Factors SPL1 and SPL12 Confer Plant Thermotolerance at Reproductive Stage

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Molecular Plant 2017年第5期10卷 735-748页

作者： Lu-Men Chao Yao-Qian Liu Dian-Yang Chen Xue-Yi Xue ying-bo mao Xiao-Ya Chen National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant Sciences Shanghai Institute of Plant Physiology and Ecology University of CAS Chinese Academy of Sciences Shanghai 200032 People's Republic of China School of Life Science and Technology ShanghaiTech University Shanghai 201210 People's Republic of China plant Science Research Center Shanghai Key Laboratory of Plant Functional Genomics and Resources Shanghai Chenshan Botanical Garden Shanghai 201602 People's Republic of China These authors contribute equally to this article

Plant reproductive organs are vulnerable to heat, but regulation of heat-shock responses in inflorescence is largely uncharacterized. Here, we report that two of the SQUAMOSA PROMOTER BINDING PROTEIN- LIKE （SPL） transcriptional factors in Arabidopsis, SPL1 and SPL12, act redundantly in thermotolerance at the reproductive stage. The spll-1 sp112-1 inflorescences displayed hypersensitivity to heat stress, whereas overexpression of SPL 1 or SPL 12 enhanced the thermotolerance in both Arabidopsis and tobacco. RNA sequencing revealed 1939 upregulated and 1479 downregulated genes in wild-type inflorescence upon heat stress, among which one-quarter （1,040） was misregulated in spll-1 sp112-1, indicating that SPL1 and SPL12 contribute greatly to the heat-triggered transcriptional reprogramming in inflorescence. Notably, heat stress induced a large number of abscisic acid （ABA） responsive genes, of which -39% were disturbed in heat induction in spll-1 sp112-1 inflorescence. Preapplication of ABA and overex- pression of SPL1 restored the inflorescence thermotolerance in spll-1 sp112-1 and in the ABA biosynthesis mutant aba2-1, but not in the pyl sextuple mutant defective in ABA receptors PYR 1/PYL 1/PYL2/PYL4/PYL5/ PYL8. Thus, inflorescence thermotolerance conferred by SPL1 and SPL2 involves PYL-mediated ABA signaling. The molecular network consisting of SPL1 and SPL12 illustrated here shed new light on the mechanisms of plant thermotolerance at the reproductive stage.

关键词： plant thermotolerance inflorescence SPL transcription factor abscisic acid

Cotton genome: challenge into the polyploidy

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Science Bulletin 2017年第24期62卷 1622-1623页

作者： Zhi-Wen Chen Jun-Feng Cao Xiu-Fang Zhang Xiao-Xia Shangguan ying-bo mao Ling-Jian Wang Xiao-Ya Chen National Key Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Plant Science Research Center Shanghai Chenshan Botanical Garden Shanghai Key Laboratory of Plant Functional Genomics and Resources

Cottons are the most important fiber crops in the *** cotton genus Gossypium has 52 species,including seven allotetraploid species and 45 *** species were domesticated and remain as crops under cultivation today:the New World allopolyploid species *** and ***(2n=52),and the Old World diploid species *** and ***(2n=26).The primary cultivated species is Upland cotton(*** L.),which accounts for more than 90%of global cotton fiber production.

关键词： Cotton genome challenge into the polyploidy

Mirids secrete a TOPLESS targeting protein to enhance JA-mediated defense and gossypol accumulation for antagonizing cotton bollworms on cotton plants

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Molecular Plant 2024年第11期17卷 1687-1701页

作者： Yu-Pei Mu Dian-Yang Chen Yu-Jie Liu Ming-Yu Zhu Xian Zhang Yin Tang Jia-Ling Lin Mu-Yang Wang Xiao-Xia Shangguan Xiao-Ya Chen Chengshu Wang ying-bo mao CAS Key Laboratory of Insect Developmental and Evolutionary Biology CAS Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyUniversity of CASChinese Academy of SciencesShanghai 200032China National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyUniversity of CASChinese Academy of SciencesShanghai 200032China Institute of Cotton Research Shanxi Agricultural UniversityYuncheng 044000China

Most coexisting insect species exhibit stunted growth compared to individual species on *** phenomenon reflects an interspecific antagonism drawing extensive attention,while the underlying mechanisms remain largely ***(Apolygus lucorum)and cotton bollworms(Helicoverpa armigera)are two common cotton *** identified a secretory protein,ASP1,from the oral secretion of mirids,found in the nucleus of mirid-infested cotton ***1 specifically targets the transcriptional co-repressor TOPLESS(TPL)and inhibits NINJA-mediated recruitment of TPL,promoting plant defense response and gossypol accumulation in cotton ***1-enhanced defense inhibits the growth of cotton bollworms on cotton plants,while having limited impact on *** mesophyll-feeding characteristic allows mirids to avoid most cotton glands,invalidating cotton *** investigation reveals the molecular mechanism by which mirids employ cotton defense to selectively inhibit the feeding of cotton bollworms.

关键词： oral secretion plant defense co-repressor TOPLESS mirids Apolygus lucorum cotton bollworms Helicoverpa armigera

Small RNAs in plant defense

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Small RNAs in plant defense

Molecular regulation of age-dependent JA response decay in Arabidopsis

第九届全国核糖核酸学术讨论会

作者：陈晓亚 Chang-Qing Yang ying-bo mao 中国科学院植物生理与生态研究所

In defense against herbivores and pathogens,plants have developed specialized(secondary)metabolic pathways and *** of secondary metabolites is often regulated by both developmental programs and stimulation *** found t...

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Molecular regulation of age-dependent JA response decay in A...

Enhanced electrochemical CO_(2)reduction coupled with urea oxidation using bifunctional atomically dispersed CuNi catalysts

2016年全国植物生物学大会

作者： ying-bo mao Yao-Qian Liu Jia-Wei Wang Xiao-Ya Chen National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and EcologyShanghai Institutes for Biological SciencesChinese Academy of Sciences

The JA signaling pathway plays important roles in plant defense against herbivorous insects. Jasmonate ZIM-domain(JAZ) proteins,which are the repressors of JA signaling,have two conserved domains: the N-terminal ZIM domain and the C-terminal Jas domain. The Jas domain is a proteinprotein interaction surface required for binding to either the transcription factors such as MYC2,or CORONATINE INSENSITIVE1(COI1),a component of the ubiquitin E3 ligase SCFCOI1. In normal conditions,the relatively high level of JAZs represses the activity of transcription factors. External stimuli,such as wounding or insect attack,cause rapid rise of JA-Ile concentration in plant cell,which triggers COI1-JAZ interaction and degradation of JAZs by the 26 S proteasome,releasing the transcription factors to activate downstream defense genes. Therefore,the abundance of JAZ proteins in cells,determined largely by the rates of protein degradation,controls the output of JA response. Here we reported that JA response decayed in an age dependent manner and this was controlled by mi R156-SPL module. mi R156 targeted SPLs dampens JA responses in leaves of adult plant by stabilizing JAZ proteins. However,leaves from adult plant actually have enhanced resistance to insect herbivore. We found contrary to JA response,the major defense compounds in plants of Brassicaceae,glucosinolates(GLSs) in leaves increase sequentially,a tendency independent of the mi R156-SPLs or the JA signaling. Thus,the two pathways change in reverse directions: while the JA-mediated inductive defense fades away,secondary metabolites deposit cumulatively. Our data provide new insight into the balanced regulation over defense systems.

关键词： JA response insect resistance JAZ proteins miR156-SPL module

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Nano Research 2025年第1期18卷 208-216页

作者： Wenjie Wu Haoyang Zhou ying Liu Yifei Pan Qingqing Chen Yu Zhang Junjie mao Wenjie Ma Ping Yu Beijing National Laboratory for Molecular Sciences Key Laboratory of Analytical Chemistry for Living BiosystemsInstitute of ChemistryChinese Academy of Sciences(CAS)Beijing 100190China College of Chemistry and Materials Science Anhui Normal UniversityWuhu 241002China University of Chinese Academy of Sciences Beijing 100049China

The electrochemical conversion of carbon dioxide(CO_(2))into chemical fuels represents a promising approach for addressing global carbon balance ***,this process is hindered by the kinetic limitations of anodic reactions,usually the oxygen evolution reaction,resulting in less efficient production of high value-added ***,we report an integrated electrocatalytic system that couples CO_(2)reduction reaction(CO_(2)RR)with urea oxidation reaction(UOR)using a bifunctional electrocatalyst with atomically dispersed dual-metal CuNi sites anchored on bamboo-like nitrogen-doped carbon nanotubes(CuNi-CNT),which were synthesized through a one-step pyrolysis *** bifunctional CuNi-CNT catalyst exhibits a near 100%CO Faraday efficiency for CO_(2)RR over a wide potential range and outstanding UOR performance with a negatively shifted potential of 210 mV at 10 mA·cm^(-2).In addition,we assemble a two-electrode electrolyzer using bifunctional CuNi-CNT-modified carbon fiber paper electrodes as both cathode and anode,capable of operating at a remarkably low cell voltage of 1.81 V at 10 mA·cm-2,significantly lower than conventional *** study provides a novel avenue to achieving an efficient carbon cycle with reduced electric power consumption.

关键词： electrochemical conversion bimetallic site catalyst bifunctional electrocatalyst carbon dioxide reduction urea oxidation