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Residues of Organochlorine Pesticides(OCPs) in Agricultural Soils of Zhangzhou City,China

Pedosphere 2012年第2期22卷 178-189页

作者： YANG Dan QI Shi-hua ZHANG Jia-Quan TAN Ling-Zhi ZHANG Jun-Peng ZHANG Yuan XU Feng XING Xin-Li HU ying CHEN Wei YANG Jun-hua XU Mei-Hui State Key Laboratory of Biogeology and Environmental Geology China University of GeoseieneesWuhan 430074(China) School of Environmental Studies China University of GeosciencesWuhan 430074(China) Geological Survey of Fujian Province Fuzhou 350013(China)

A soil survey was conducted in Zhangzhou City, an important agricultural region in south of the Fujian Province, China. 93 surface soil samples were collected in the paddy fields, vegetable lands, orchards and tea plantations from Zhangzhou City. An additional soil profile was sampled in a paddy field as previous research had indicated high concentrations of organochlorine pesticides （OCPs） in the paddy fields. Dichlorodiphenyltrichloroethanes （DDTs） ranged from 0.64 78.07 ng g-1 dry weight and hexachlorocyclohexanes （HCHs） ranged from 0.72-30.16 ng g-1 dry weight in the surface soil of the whole study region. Ratios of a-HCH/-γ-HCH 〈 4 and o,p′-DDT/p,p′-DDT 〉 1 in all soil samples suggested that lindane and dicofol were widely applied in this region in the past. Concentrations of HCHs and DDTs in soils from the four land use types followed the orders： paddy fields 〉 vegetable lands 〉 tea plantations 〉 orchards and tea plantations 〉 orchards 〉 paddy fields 〉 vegetable lands, respectively. Analyses of the data showed no correlation （r 〈 0.1） between elevation and OCPs contents in paddy fields, orchards and vegetable lands, indicated no significantly different features in distribution of HCHs and DDTs in the soils from low lying plains and mountains and the unsystematic usage of OCPs, and highlighted the fragmented nature of agricultural production in Zhangzhou, as well as the reemission of OCPs from the soils, where high OCPs concentrations were found, in Longhai of Zhangzhou. In addition, no obvious relationship between the OCPs and total organic carbon （TOC）（r 〈 0.3） was observed in the soil profile. The mean contribution of dicofol in total DDTs was 66% in the whole Zhangzhou region. The approximate burdens of HCHs and DDTs in the surface layer of 0-20 em were 0.44 and 1.55 t, respectively. The storage of both HCHs and DDTs in soil surface layer （0-20 cm） accounts for 40% burden of the soil layer of 0 50 cm （1.10 t HCHs and 3.87 t DDTs）, in which the highest conc

关键词： dichlorodiphenyltrichloroethanes (DDTs) hexachlorocyclohexane (HCHs) landform land use types vertical distribution

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基于矿物元素指纹图谱鉴定陈皮产地的方法研究

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分析测试学报 2024年第2期43卷 301-308页

作者：赖晓娜罗辉泰张春华黄锦沛陈怡珊李乐周熙黄芳吴惠勤广东省科学院测试分析研究所(中国广州分析测试中心) 广东省化学测量与应急检测技术重点实验室广东省中药质量安全工程技术研究中心广东广州510070 江门市新会区动物防疫监督所(江门市新会区农产品质量安全监督检验测试中心) 广东江门529199

采用电感耦合等离子体质谱法测定了新会、广西、湖南3地42批陈皮样品中51种矿物元素的含量,运用方差分析和偏最小二乘法-判别分析(PLS-DA)研究了不同产地陈皮中矿物元素的差别,以筛选得到的9种元素为溯源指标,将不同数量级矿物元素含量... 详细信息

采用电感耦合等离子体质谱法测定了新会、广西、湖南3地42批陈皮样品中51种矿物元素的含量,运用方差分析和偏最小二乘法-判别分析(PLS-DA)研究了不同产地陈皮中矿物元素的差别,以筛选得到的9种元素为溯源指标,将不同数量级矿物元素含量进行分类和系数缩放后,构建了陈皮中矿物元素的指纹图谱,并基于各产地矿物元素含量的平均值构建了新会、广西、湖南产地陈皮中矿物元素的标准指纹图谱。通过采用SPSS20.0计算18批新会陈皮矿物元素指纹图谱与其标准指纹图谱的相似度,确定新会陈皮矿物元素指纹图谱的相似度阈值为0.941,基于此阈值建立了新会陈皮的鉴别模型。采用24批样品对所建立的鉴别模型进行准确性验证,正确率为91.6%。该方法操作简单直观,满足实验分析要求,可为陈皮的产地溯源提供参考。

关键词：陈皮矿物元素指纹图谱产地鉴别电感耦合等离子体质谱法(ICP-MS)

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混杂纤维混凝土的力学性能及微观分析

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混凝土与水泥制品 2024年第4期 59-62页

作者：霍曼琳杨国栋曹玉新靳利安聂华兰州交通大学土木工程学院甘肃兰州730070 中电建铁路建设投资集团有限公司北京100070 中国水利水电第七工程局有限公司四川成都611730

通过L9(33)正交试验研究了玄武岩纤维(BF)、聚丙烯纤维(PPF)和粉煤灰的掺量对混凝土力学性能的影响,并进行了微观分析。结果表明:混掺适量PPF和BF,同时掺入适量粉煤灰可有效改善混杂纤维混凝土的力学性能及微观结构;综合考虑,推荐PPF、B... 详细信息

通过L9(33)正交试验研究了玄武岩纤维(BF)、聚丙烯纤维(PPF)和粉煤灰的掺量对混凝土力学性能的影响,并进行了微观分析。结果表明:混掺适量PPF和BF,同时掺入适量粉煤灰可有效改善混杂纤维混凝土的力学性能及微观结构;综合考虑,推荐PPF、BF、粉煤灰的掺量分别为0.10%、0.10%、20%。

关键词：玄武岩-聚丙烯混杂纤维粉煤灰混凝土力学性能微观结构

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血浆miR-320c、miR-126及miR-34c联合检测对慢性阻塞性肺疾病严重程度的预测价值

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临床检验杂志 2024年第3期42卷 182-185页

作者：金花胡志雄周海英刘晓鹏王静金权潘春峰陆徐峰复旦大学附属金山医院呼吸科上海201508

目的探讨血浆miRNA水平与慢性阻塞性肺疾病(COPD)严重程度的相关性。方法利用荧光定量PCR(qPCR)检测健康人群(10例)及COPD患者(10例)血浆miRNA的表达水平,筛选与COPD患者密切相关的miRNA。进一步在健康人群(28例)和COPD慢性阻塞性肺疾... 详细信息

目的探讨血浆miRNA水平与慢性阻塞性肺疾病(COPD)严重程度的相关性。方法利用荧光定量PCR(qPCR)检测健康人群(10例)及COPD患者(10例)血浆miRNA的表达水平,筛选与COPD患者密切相关的miRNA。进一步在健康人群(28例)和COPD慢性阻塞性肺疾病全球创议(GOLD)Ⅰ级(30例)、Ⅱ级(32例)、Ⅲ级(30例)及Ⅳ级(28例)患者中明确候选miRNA单独及联合检测对COPD严重程度的预测价值。结果COPD患者血浆miR-320c、miR-126及miR-34c水平显著高于健康人群(P<0.01)。随着COPD级别的增加,血浆miR-320c、miR-126及miR-34c的表达水平亦增加。miR-320c、miR-126、miR-34c单独及联合检测预测中重度COPD患者的ROC曲线下面积(AUC^(ROC))分别为0.822、0.705、0.783及0.892。结论血浆miR-320c、miR-126及miR-34c可预测COPD严重程度,其联合检测的预测效能显著高于单项miRNA。

关键词： miR-320c miR-126 miR-34c 慢性阻塞性肺疾病分级

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Identification of SSR loci in Betula luminifera using birch EST data

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Journal of Forestry Research 2011年第2期22卷 201-204页

作者： LU Yong-quan LI Hai-ying JIA Qing huaNG hua-hong TONG Zai-kang The Nurturing Station for the State Key Laboratory of Subtropitical Silviculture Zhejiang Agriculture and Forestry University Lin＇an 311300 China

Expressed sequence tags（ESTs） are generated from single-pass sequencing of randomly picked cDNA clones and can be used for development of simple sequence repeat（SSR） markers or ***,EST databases have been developed for only a small number of *** paper provides a case study of the utility of freely available birch EST resources for the development of markers necessary for the genetic analysis of Betula *** on birch EST data,primers for 80 EST-SSR candidate loci were developed and tested in *** these,59 EST-SSR loci yielded single,stable and clear PCR *** then tested the utility of those 59 markers in *** results showed 28（47.6%） yielded stable and clear PCR products for at least one *** *** addition,this study describes a rapid and inexpensive alternative for the development of SSRs in species with scarce available sequence data.

关键词： exploit Betula luminifera birch EST database EST-SSR

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Surgical treatment of pelvic fracture urethral distraction defects in boys:which approach is suitable?

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Asian Journal of Andrology 2020年第3期22卷 292-295页

作者： Lin Wang Hai-Lin Guo Hui-Quan Shu Jie Gu Chong-Rui jin Fang Chen ying-Long Sa Department of Urology Shanghai Jiao Tong University Affiliated Sixth People's HospitalShanghai 200233China Department of Urology Shanghai Children's HospitalShanghai Jiao Tong UniversityShanghai 200062China Shanghai Eastern Institute of Urologic Reconstruction Shanghai 200233China

Pelvic fracture urethral distraction defects(PFUDDs)are relatively infrequent in boys,and treatment for PFUDDs presents one of the most difficult problems in urological *** urethroplasty is considered an ideal surgical procedure for PFUDDs in ***,various surgical approaches for anastomotic urethroplasty have been proposed,including a simple transperineal approach,a tran sperineal in tercorporal septal separati on approach,a tran sperineal in ferior pubic approach,and a combined transpubic・perineal *** study aims to determine which surgical approach is best for PFUDDs in *** retrospectively identified 22 boys with PFUDDs aged 2-14 years who underwent anastomotic urethroplasty via different approaches between January 2008 and December ***-up was performed in all the 22 patients for 6-123(mean:52.0)***,20 of the 22 boys(90.9%)were successfully treated,including 1 of 2 patients treated with a simple transperineal approach,3 of 3 with a transperineal approach with intercorporal septal separation,14 of 15 with a transperineal inferior pubic approach,and 2 of 2 with a combined transpubic-perineal *** patients had failed outcomes after the operation,and stenosis *** on the outcome of the 22 patients,we can draw a preliminary conclusion that most boys(20/22)can be treated with a transperineal inferior pubic approach or simpler procedures without the need of completely removing or incising the *** combined transpubic-perineal approach can be used in cases of extremely long urethral distract defects.

关键词： children pelvic fracture posttraumatic urethra urethral stenosis

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Achievements and challenges of primate conservation in China

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Zoological Research(Diversity and Conservation) 2024年第1期1卷 66-74页

作者： Bao-Guo Li He Zhang Ming Li Xue-Long Jiang Peng-Fei Fan Jiang Zhou Song-Tao Guo Xiao-Guang Qi jin-hua Li Ji-Qi Lu Dong-Po Xia Liang-Wei Cui Zuo-Fu Xiang Qi-Hai Zhou Zhi-Pang huang Cheng-Ming huang Wen Xiao Hui-Jian Hu Zhi-Xin Zhou Ming-Yong Chen Da-Yong Li Peng-Lai Fan Yin Yang Ru-Liang Pan Shaanxi Key Laboratory for Animal Conservation College of Life SciencesNorthwest UniversityXi’anShaanxi 710069China Jiangxi Provincial Key Laboratory of Conservation Biology College of ForestryJiangxi Agricultural UniversityNanchangJiangxi 330045China Key Laboratory of Animal Ecology and Conservation Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China State Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650201China School of Life Sciences Sun Yat-sen UniversityGuangzhouGuangdong 510275China School of Karst Science Guizhou Normal UniversityGuiyangGuizhou 550025China International Collaborative Research Center for huangshan Biodiversity and Tibetan Macaque Behavioral Ecology Anhui UniversityHefeiAnhui 230601China Institute of Biodiversity and Ecology Zhengzhou UniversityZhengzhouHenan 450001China Key Laboratory for Conserving Wildlife with Small Populations in Yunnan Southwest Forestry UniversityKunmingYunnan 650224China College of Life Sciences and Technology Central South University of Forestry and TechnologyChangshaHunan 410004China Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection(Ministry of Education) Guangxi Normal UniversityGuilinGuangxi 541004China International Centre of Biodiversity and Primate Conservation Centre Dali UniversityDaliYunnan 671003China Guangdong Key Laboratory of Animal Conservation and Resource Utilization Institute of ZoologyGuangdong Academy of SciencesGuangzhouGuangdong 510663China School of Life Sciences Yunnan UniversityKunmingYunnan 650500China Key Laboratory of Southwest China Wildlife Resources Conservation(Ministry of Education) China West Normal UniversityNanchongSichuan 637001China Institute of International Rivers and Eco-security Yunnan UniversityKunmingYunnan 650500China School of Human Sciences The University of Western AustraliaPerthWA 6009Australia

The dual impact of climate change and human activities has precipitated a sharp decline in primate biodiversity *** is home to the most diverse primate species in the Northern hemisphere,which face severe ecological threats due to the expansion of modern agriculture,extensive exploitation and consumption of natural resources,and excessive land development during its transition from an agricultural to a modern *** response,China has implemented various ecological conservation measures,including habitat restoration and *** efforts have made substantial strides in biodiversity conservation,with certain regions witnessing an increase in primate *** the current study,we conducted a systematic review of historical documents and field research data related to Chinese primates,evaluating the endangered status of primate species in *** improvements in the habitats of most primate species and some population growth,many species still face severe threats,including declining and small *** such as the Myanmar snub-nosed monkey(Rhinopithecus strykeri),eastern black crested gibbon(Nomascus nasutus),and Hainan gibbon(***)remain particularly vulnerable due to their limited distribution ranges and extremely small *** scientific data,fragmented information,and not enough studies in conservation biology further compound the ***,there is a notable lack of detailed population monitoring data for species such as the Bengal slow loris(Nycticebus bengalensis),pygmy slow loris(***),Indochinese gray langur(Trachypithecus crepusculus),Shortridge’s langur(***),and capped langur(***),which hinders the development of practical and targeted conservation management ***,for national biodiversity conservation,there is an urgent need for specialized primate surveys,enhancing habitat protection and restoration,and increasing focus on cross-border conse

关键词： Primates Biodiversity Habitat fragmentation Habitat restoration Conservation strategies

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Decreasing detection frequency of MITE(MCLas-A)in the population of‘Candidatus Liberibacter asiaticus'recently collected in southern China

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Journal of Integrative Agriculture 2020年第10期19卷 2597-2601页

作者： CUI Xue-jin ZENG Chun-hua LIU Ke-hong TENG Cai-ling ZHOU Chang-yong WANG Xue-feng National Citrus Engineering Research Center Citrus Research InstituteChinese Academy of Agricultural Sciences/Southwest UniversityChongqing 400712P.R.China

An active miniature inverted-repeat transposable element(MITE),MCL as-A,was previously identified from'Candidatus Liberibacter asiaticus'known to be associated with citrus huanglongbing(HL B,yellow shoot disease).To explore the recent transposition status of MCLas-A,389'***'strains collected from nine regions in China were amplifed using a specific primer set and three representative'***'strains were analyzed by next-generation sequencing(NGS)*** and genomic analysis showed that the entire MCLas A was only present in 1.80%(7/389)and the jumping-out type of the MITE was predominant(8123%)in samples tested,suggesting high frequency transposition occurred in'***'strains recently collected from *** roles of transposition of the active MITE remain to be determined.

关键词： huanglongbing Candidatus Liberibacter asiaticus' miniature inverted-repeat transposable element MITE

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不同授粉强度对蓝莓柱头响应机制的转录组分析

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中国农业科技导报 2024年第10期26卷 71-82页

作者：冀薇樊莹黄家兴杨慧鹏徐进李小英郭岳琴吴跃国李继莲姚军中国农业科学院蜜蜂研究所北京100093 贵州省畜禽遗传资源管理站贵阳550001 罗平县云岭蜂业工贸有限公司云南曲靖655899

授粉对蓝莓生产至关重要,为探究不同授粉强度下柱头的响应机制及其特性,以‘蓝源(M7)’为研究对象,比较不同花龄下柱头可授性,并对花粉活力进行测定,然后对3种授粉强度处理下的蓝莓柱头进行转录组测序和分析。结果表明,随花龄的不断延长... 详细信息

授粉对蓝莓生产至关重要,为探究不同授粉强度下柱头的响应机制及其特性,以‘蓝源(M7)’为研究对象,比较不同花龄下柱头可授性,并对花粉活力进行测定,然后对3种授粉强度处理下的蓝莓柱头进行转录组测序和分析。结果表明,随花龄的不断延长,‘蓝源(M7)’柱头可授性表现为先升高后逐渐降低的趋势,在开花第2天可授性最强。3种授粉强度的花粉表现出不同的活力,随着授粉强度的增加,柱头内参与花粉管生长调节的钙依赖蛋白激酶17(Cpk17)和涉及肌醇含量的肌醇加氧酶(MIOX1)等基因的表达量显著提高。对差异表达基因的GO(gene ontology)富集分析发现,与碳水化合物代谢、肌醇加氧酶活性和细胞壁组织相关的功能基因被显著富集;KEGG(kyoto encyclopedia of genes and genomes)富集分析发现,与植物−病原体互作和肌醇膦酸代谢相关的通路被显著富集。由此推测,授粉强度的增加可能对柱头和花粉的识别过程产生影响,通过参与柱头和花粉间的信号转导、激素合成过程对花粉管的生长起促进作用,进而影响受精过程。以上结果为理解蓝莓柱头对不同授粉强度的响应机制提供了理论参考,为蓝莓的精确授粉提供科学依据。

关键词：蓝莓柱头授粉强度转录组测序差异表达基因

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野生和养殖锦鲤肠道微生物多样性及功能预测

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山东农业大学学报（自然科学版） 2024年第2期55卷 218-227页

作者：赵园园古应慧孟金柱梁正其梅杰宋兴超巴家文铜仁学院贵州省梵净山地区生物多样性保护与利用重点实验室贵州铜仁554300

为探究不同生活环境下锦鲤肠道微生物的差异,本研究采用16S rRNA高通量测序技术对野生和养殖锦鲤肠道微生物组成进行分析。结果表明,锦鲤肠道微生物中的优势门为变形菌门(Proteobacteria,29.81%)、蓝细菌门(Cyanobacteria,25.57%)、绿... 详细信息

为探究不同生活环境下锦鲤肠道微生物的差异,本研究采用16S rRNA高通量测序技术对野生和养殖锦鲤肠道微生物组成进行分析。结果表明,锦鲤肠道微生物中的优势门为变形菌门(Proteobacteria,29.81%)、蓝细菌门(Cyanobacteria,25.57%)、绿弯菌门(Chloroflexi,18.16%);不同环境下锦鲤肠道菌群在数量和群落结构上都有明显差异,在属水平上,养殖环境下优势菌属有红细菌属(Rhodobacter)、藤黄色杆菌属(Luteolibacter)、泉发菌属(Crenothrix),野生环境下优势菌属有绿丝菌属(Chloronema)、纤发鞘丝蓝细菌属(Leptolyngbya)、暖绳菌属(Caldilinea);在种水平上,养殖环境下优势菌有Methylobacterium adhaesivum、琥珀黄杆菌(Flavobacterium succinicans)、胭脂甲杆菌(Methylobacterium komagatae)、湖泊玫瑰单胞菌(Roseomonas lacus);野生环境下优势菌有空腔污水球菌(Defluviicoccus vanus)、Proteocatella sphenisci、湖泊玫瑰单胞菌、Roseomonas massiliensis、索氏鲸杆菌(Cetobacterium somerae);α多样性分析显示养殖环境下锦鲤肠道微生物的多样性以及物种丰富度均比野生样本高;肠道微生物功能预测显示,L1、L2、L3水平最富集的KEGG通路分别是新陈代谢、氨基酸代谢和萜类和类固醇的生物合成,不同环境下差异显著的KEGG通路包括2-氧羧酸添加代谢、甜菜碱生物合成、安莎霉素的生物合成、双酚降解、丁酸盐代谢等。本研究从肠道微生物角度探究了锦鲤的环境适应性,为其健康养殖提供了新思路。

关键词：锦鲤环境肠道微生物功能预测

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