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A Study of the Distribution of Apple stem pitting virus in Tissues of Pear Tree Using In Situ Hybridization and In Situ RT-PCR

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Agricultural Sciences in China 2009年第11期8卷 1351-1359页

作者： ZHAO ying LIU Na niu Jian-xin Horticultural Department Agricultural College Shihezi University Shihezi 832003 P.R. China

To understand the distribution of Apple stem pitting virus （ASPV） in tissues of pear tree and provide directly theorical and technical support for shoot tips detoxication, leaves and shoot tips of Korla pear were used as the materials, cDNA probe for ASPV was synthesized through RT-PCR reaction system using the unradioactive digoxigenin-labeled probe, and the specificity and sensitivity of probe were verified by blot hybridization method. Paraffin slice for in situ PCR and in situ hybridization was made and the location and distribution of ASPV RNA were detected in paraffin slices using in situ reverse transcription polymerase chain reaction, and the important factors which influenced the experiment results were optimized. ASPV mainly distributed in palisade tissue of mesophyll cells, external cortex of the tip, and the corresponding newborn vascular bundles. 20 min was the suitable digestive time for proteinase K. For the better amplication, RT reaction system should be above 0.2 U μL^-1 and 0.4 mmol L^-1 for RNasin and dNTPs respectively, 0.1-1.3 U μ L^-1 SuperScript Ⅱ, 0.6- 0.8 μmol L^-1 primer concentration, and above 0.5 U 100 μL^-1 LA Taq DNA polymerase. The suitable annealing temperature in PCR reaction was 60℃ with 35 cycles. The apical meristem of 0.25 mm was the region of virus-free.

关键词： Korla pear Apple stem pitting virus cDNA probe digoxigenin in situ PCR

Relative fitness of susceptible and Cry1A.105/Cry2Ab2-single-/dual-protein-resistant Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) on non-Bt diet and a diet containing a low concentration of two proteins

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Insect Science 2023年第2期30卷 398-410页

作者： Shucong Lin Graham Head Paula Price ying niu Fangneng Huang Department of Entomology Louisiana State University Agricultural CenterBaton RougeLouisianaUSA Bayer Crop Science ChesterfieldMissouriUSA

Helicoverpa zea(Boddie)is a destructive agricultural pest species that is targeted by both Bacillus thuringiensis(Bt)maize and cotton in the United ***1A.105 and Cry2Ab2 are two Bt proteins expressed in a widely planted maize event MON *** this study,two tests(Test-Ⅰ and Test-Ⅱ)were conducted to evaluate the relative fitness of Bt-susceptible and-resistant *** on non-Bt diet(Test-Ⅰ and Test-Ⅱ)and a diet containing a mix of Cry1A.105 and Cry2Ab2 at a low concentration(Test-Ⅱ only).Insect populations evaluated in Test-Ⅰ were two Bt-susceptible strains and three Bt-resistant strains(a single-protein Cry1A.105-,a single-protein Cry2Ab2-,and a dual-protein Cry1A.105/Cry2Ab2-resistant strains).Test-Ⅱ analyzed the same two susceptible strains,three backcrossed-and-reselected Cry1A.105/Cry2Ab2-single-/dual-protein-resistant strains,and three F1 heterozygous *** of life table parameters showed that neither the single-nor dual-protein Cry1A.105/Cry2Ab2 resistance in *** was associated with fitness costs under the test *** single Cry protein resistances at a concentration of a mix of Cry1A.105 and Cry2Ab2 that resulted in a zero net reproductive rate for the two susceptible strains were functionally incomplete recessive or codominant,and the dual-protein resistance was completely *** lack of fitness costs could be a factor contributing to the rapid revolution of resistance to the Cry proteins in this *** generated from this study should aid our understanding of Cry protein resistance evolution and help in refining IRM programs for ***.

关键词： resistance management Cry1A.105 Cry2Ab2 fitness costs Helicoverpa zea low dose effect

Experimental study on rotary longitudinal-torsional ultrasonic machining of unidirectional CFRP

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Chinese Journal of Aeronautics 2024年第11期37卷 517-534页

作者： Ziqiang ZHANG Feng JIAO Yuanxiao LI Xue WANG ying niu Jinglin TONG School of Mechanical and Power Engineering Henan Polytechnic UniversityJiaozuo 454003China

Addressing the challenges of high grinding temperature,poor hole quality,and tool rod blocking in core drilling of carbon fiber reinforced plastics(CFRP),this study investigates the material removal characteristic of rotary longitudinal-torsional ultrasonic machining(RLTUM)of CFRP based on the Hertz contact *** study also conducts relevant experiments to compare and analyze the grinding force,grinding temperature,hole wall quality,exit quality,and tool wear in ordinary core drilling(OCD),rotary ultrasonic machining(RUM),and RLTUM of *** results demonstrate that RLTUM significantly reduces the grinding force,grinding temperature,hole exit damage and improves the surface smoothness of the CFRP hole *** utilizing intermittent cutting in circumferential direction for torsional vibration,RLTUM enables automatic removal of rod chips while restraining tool chip adhesion and reducing heat generation as well as tool ***,RLTUM shows great potential for enhancing the quality of holemaking in CFRP.

关键词： Carbon fiber reinforced plastics(CFRP) Rotary longitudinal-torsional ultrasonic machining Grinding force Grinding temperature Hole quality Tool wear

Sulfonated poly(4-vinylpyridine) heteropolyacid salts:A reusable green solid catalyst for Mannich reaction

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Chinese Chemical Letters 2014年第4期25卷 575-578页

作者： Wen-You Li ying-Xiao Zong Jun-Ke Wang Yu-ying niu Department of Chemical Engineering Jiuquan Vocational and Technical College Key Laboratory of Hexi Corridor Resources Utilization of Gansu Universities College of Chemistry and Chemical EngineeringHexi University Gansu Engineering Laboratory of Applied Mycology Hexi University

Sulfonated poly（4-vinylpyridine） heteropolyacid salts acted as a heterogeneous catalyst to effectively catalyze the one-pot synthesis of β-amino carbonyl compounds via the Mannich reaction between aromatic aldehydes, aromatic ketone, and aromatic amines. In addition, the catalyst could be easily recovered by the filtration and reused six times without significant loss of catalytic activity.

关键词： Mannich reaction Heteropolyacid Poly(4-vinylpyridine) Heterogeneous catalyst

High Precision Prediction of Rolling Force Based on Fuzzy and Nerve Method for Cold Tandem Mill

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Journal of Iron and Steel Research International 2008年第2期15卷 23-27页

作者： JIA Chun-yu SHAN Xiu-ying niu Zhao-ping College of Mechanical Engineering Yanshan University Qinhuangdao 066004 Hebei China

The rolling force model for cold tandem mill was put forward by using the Elman dynamic recursive network method,based on the actual measured ***,a good assumption is put forward,which brings a full universe of discourse self-adjusting factor fuzzy control,closed-loop adjusting,based on error feedback and expertise into a rolling force prediction model,to modify prediction outputs and improve prediction precision and *** simulated results indicate that the method is highly effective and the prediction precision is better than that of the traditional *** relative error is less than ±4%,so the prediction is high precise for the cold tandem mill.

关键词： Elman dynamic recursive network fuzzy control cold tandem mill rolling force prediction

Analysis of Atmospheric Turbidity in Clear Skies at Wuhan,Central China

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Journal of Earth Science 2017年第4期28卷 729-738页

作者： Lunche Wang Yisen Chen ying niu German Ariel Salaza Wei Gong Department of Geography School of Earth SciencesChina University of GeosciencesWuhan 430074China Physics Department School of Exact SciencesSalta National UniversitySalta A4408FVYArgentina State Key Laboratory of Information Engineering in Surveying Mapping and Remote SensingWuhan UniversityWuhan 430079China

The Angstr6m turbidity coefficient （β） and Linke turbidity factor （TL） are used to study the atmospheric conditions in Wuhan, Central China, using measureβd direct solar radiation during 2010-2011 in this study. The results show that annual mean β values generally increase from 0.28 in the morning to 0.35 at noon, and then decrease to 0.1 in the late afternoon during the day; annual mean TL generally varies from 3 to 7 in Central China. Both turbidity coefficients have maximum values in spring and summer, while minimum values are observed in winter months. It also reveals that β values show preponderance （52.8%） between 0.15 and 0.35, 78.1% of TL values are between 3.3 and 7.7, which can be compared with other sites around the world. Relationship between turbidity coefficients and main me- teorological parameters （humidity, temperature and wind direction） have been further investigated, it is discovered that the local aerosol concentrations, dust events in northern China and Southwest Monsoon from the Indian Ocean influences the β values in the study area.

关键词： direct solar radiation AngstrOm turbidity coefficient Linke turbidity factor Central China.

Scalable high performance de-duplication backup via hash join

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Journal of Zhejiang University-Science C(Computers and Electronics) 2010年第5期11卷 315-327页

作者： Tian-ming YANG Dan FENG Zhong-ying niu Ya-ping WAN Wuhan National Laboratory for Optoelectronics School of Computer Science and Technology Huazhong University of Science and Technology Wuhan 430074 China

Apart from high space efficiency,other demanding requirements for enterprise de-duplication backup are high performance,high scalability,and availability for large-scale distributed *** main challenge is reducing the significant disk input/output(I/O) overhead as a result of constantly accessing the disk to identify duplicate *** inline de-duplication approaches mainly rely on duplicate locality to avoid disk bottleneck,thus suffering from degradation under poor duplicate locality *** paper presents Chunkfarm,a post-processing de-duplication backup system designed to improve capacity,throughput,and scalability for *** performs de-duplication backup using the hash join algorithm,which turns the notoriously random and small disk I/Os of fingerprint lookups and updates into large sequential disk I/Os,hence achieving high write throughput not influenced by workload *** importantly,by decentralizing fingerprint lookup and update,Chunkfarm supports a cluster of servers to perform de-duplication backup in parallel;it hence is conducive to distributed implementation and thus applicable to large-scale and distributed storage systems.

关键词： Backup system De-duplication Post-processing Fingerprint lookup Scalability

Life cycle of Chetoneura shennonggongensis （Diptera： Keroplatidae： Keroplatinae） from Jiangxi Province, China

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Insect Science 2009年第4期16卷 351-359页

作者： Xue-Zhen Li Chang-ying niu Qiu-ying Huang Chao-Liang Lei David W. Stanley Key Laboratory of Utilization of Insect Resources and Sustainable Control of Pests Huazhong Agricultural University Wuhan China Agricultural Research Service Biological Control of Insects Research Laboratory Columbia USA

We report the first description of the biology of non-bioluminescent Keroplatidae from China. The life cycle of Chetoneura shennonggongensis is documented from laboratory culture and from field observations. The larval stage usually lasts 8-10 months. Large amounts of pupae are observed in late June, where they remain suspended horizontally or vertically from silk threads. The pupal suspending postures differ from other Keroplatidae species. Sexual differentiation is evident from the pupal stage. July is the peak time of adult emergence. Mating usually takes place immediately upon female emergence if adult males are available. The adult life span is short. Females live a maximum of 5 days and males 7 days. Egg development time varies from 20 30 days depending on environmental temperature. Larvae hang long sticky silk threads with a series of mucous droplets to capture prey. Some behaviors associated with snare construction and prey capture are also described.

关键词： Chetoneura shennonggongensis Chinese Keroplatidae life cycle

Severe and delayed immune-mediated hemolysis post-liver transplantation

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Hepatobiliary & Pancreatic Diseases International 2017年第6期16卷 665-668页

作者： Quan Zhuang Hong Liu Ke Cheng ying niu Xing-Guo She ying-Zi Ming Transplantation Center of the Third Xiangya Hospital Central South University Changsha 410013 China

To the Editor:Hemolysis,which is caused by a variety of immune and non-immune mechanisms,is a well-recognized complication of solid organ transplantation.;Hemolysis post-liver transplantation can be induced by drug,in... 详细信息

关键词： RBC Severe and delayed immune-mediated hemolysis post-liver transplantation PLS DCD EBV