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World Journal of Gastroenterology 2009年第12期15卷 1528-1529页

作者： yan yin li-Hua He Jian-Zhong Zhang Department of Diagnosis National Institute for-Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China

Helicobacter pylori(H pylori),a gastric pathogen,is a major cause of chronic gastritis and peptic ulcer disease,and is an important risk factor for the development of gastric *** of the bacterium from gastric biopsy is essential for the determination of drug resistance of H ***,the isolation rates of H pylori from infected individuals vary from 23.5%to 97% due to a number of factors such as biopsy preparation,cultural environment,medium and the method *** the present case,we found that a prolonged incubation period of up to 19 d allowed successful isolation of H pylori from a patient who received triple therapy that failed to eradicate the bacterium.

关键词： Helicobacter pylori Isolation Eradication

Expression and clinical significance of S100A2 and p63 in esophageal carcinoma

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World Journal of Gastroenterology 2009年第33期15卷 4183-4188页

作者： li-Yu cao Yu yin Hao li yan Jiang Hong-Fu Zhang Department of Pathology Anhui Medical University Hefei 230032 Anhui Province China

AIM： TO investigate the expression and clinical significance of S100A2 mRNA and protein, p63 protein in esophageal squamous cell carcinoma （ESCC） and their roles in carcinogenesis and progression of esophageal carcinoma （EC）. METHODS： Immunohistochemical staining （S-P method） for S100A2 and p63 protein were performed in 40 samples of ESCC and 40 samples of normal esophageal mucosa. In situ hybridization （ISH） was used to detect the expression of S100A2 mRNA. RESULTS： Expression of S100A2 mRNA in ESCC was positive in 77.5% of samples, which was lower than that in normal mucosa （100%） by ISH （P = 0.002）. The expression level of S100A2 mRNA was closely related to differentiation and and node-metastasis （P = 0.012, P = 0.008）. Expression of $100A2 protein was positive in 72.5% of ESCC samples and expression of p63 protein was positive in 37.5% of ESCC samples, and was lower than that in normal mucosa （100%）（P = 0.000）. The expression of S100A2 protein was correlated with the differentiation and node-metastasis （P = 0.007, P = 0.001）, but no relationship was observed between the expression of p63 protein and clinical pathological manifestations. S100A2 protein was positively correlated with the expression of S100A2 mRNA, and negatively associated with the expression of p63 protein （P = 0.000, P = 0.002）. CONCLUSION： S100A2 and p63 protein both play important roles in the carcinogenesis of ESCC. An investigation into the combined expression of S100A2 and p63 may be helpful in early diagnosis and in evaluating the prognosis of ESCC.

关键词： Immunohistochemistry In situ hybridization Esophageal carcinoma p63 S100A2

Effects of transferred NK4 gene on proliferation, migration, invasion and apoptosis of human prostate cancer DU145 cells

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Asian Journal of Andrology 2010年第3期12卷 381-389,I0010页

作者： Dan Yue Yong Wang Ping Ma yin-yan li Hong Chen Ping Wang Chang-Shan Ren Cancer Research Institute First Affiliated Hospital China Medical University Shenyang 110001 China Department of Urology Fourth Affiliated Hospital China Medical University Shenyang 110032 China Department of Ultrasound First Affihated Hospttal China Medical University Shenyang 110001 China

We investigated the ability of NK4, an antagonist of human hepatocyte growth factor （HGF）, to inhibit the influence of HGF on proliferation, migration, invasion and apoptosis of human prostate cancer cells. Expression vector pBudCE4.1-EGFP-NK4 containing NK4 cDNA was used to transfect human prostate cancer DU145 cells, and the effects of the autocrine NK4 on tumor cell proliferation, migration, invasion and apoptosis were assessed in vitro. in vivo, we subcutaneously implanted DU145 cells, mock-transfected clone （DU145/empty vector） cells and NK4- transfected clone （DU145/NK4） cells into nude mice, and then evaluated tumor growth, cell proliferation and cell apoptosis in vivo. We found that DU145/NK4 cells expressed NK4 protein. In the in vitro study, autocrine NK4 at- tenuated the HGF-induced tumor cell proliferation, migration and invasion, and stimulated apoptosis. Furthermore, autocrine NK4 effectively inhibited the HGF-induced phosphorylation of c-Met, extracellular signal-regulated kinase-1 （ERK1）. and protein kinase B 1/2 （Aktl/2）. Histological examination revealed that autocrine NK4 inhibited prolifera- tion and accelerated apoptosis of prostate cancer cells. These results show that genetic modification of DU145 cells with NK4 cDNA yields a significant effect on their proliferation, migration, invasion and apoptosis. Molecular targeting of HGF/c-Met by NK4 could be applied as a novel therapeutic approach to prostate cancer.

关键词： hepatocyte growth factor human prostate cancer NK4 DU 145 cells

Trehalose and trehalose-hydrolyzing enzyme in the haemolymph of Locusta rnigratoria infected with Metarhizium anisopliae strain CQMa102

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Insect Science 2007年第4期14卷 277-282页

作者： HUA ZHAO ZHONG-KANG WANG YOU-PING yin yan-liNG li ZHEN-LUN li GUO-XIONG PENG YU-XIAN XIA GeneticEngineeringResearchCenter CollegeofBioengineeringChongqingUniversityChongqingChina

Topical application of the Metarhizium anisopliae var. acridum specialist strain CQMa 102 to the locust Locusta migratoria manilensis results in changes of the concentrations of trehalose and glucose in the haemolymph. Micrographs of the locust haemolymph shows Metarhizium anisopliae can effectivly penetrate the external skeleton of locust and after 2 days infection, the hyphae body will appear in the haemolymph of infected insects. The time in decrease of trehalose concentration coincided with that in increase of trehalose-hydrolysing enzyme activity in the haemolymph of the fungus-infected insects. Overlay gel analysis indicated there was considerably more trehalose-hydrolysing activity in the haemolymph of locusts infected by fungus than in controls. A comparable isoform was identified in in vitro culture of the fungus, suggesting a fungal origin for the in vivo enzyme. Haemolymph trehalose decreased significantly during mycosis of locusts by M. anisopliae. All these results suggested that this fungus may take advantage of competing nutrient utilization against the insect by its trehalose-hydrolyzing enzyme secretion. It may provide fundamental knowledge for fungal pathogenesis.

关键词： Metarhizium anisopliae Locusta migratoria manilensis trehalose-hydrolysingenzymes trehalose glucose mycosis

Research on IGBT Drive Circuit Based on 2QD30A17K-I Driver

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电力电子技术 2012年第12期46卷 60-61,66页

作者： HE Wei-wei WANG Zhi-han FU Jun-yin li yan-fei Shenzhen Bronze Technologies Ltd. Shenzhen 518057 China

The insulated gate bipolar transistor(IGBT) has become the most popular controllable power switching device in the design of power electronics *** the interface of power circuit and control circuit in power source, the IGBT drive circuit should be with short transition time and the function of isolating safely and monitoring correctly the short-circuit and over-voltage *** paper presents the short-circuit protection theory and relative parameter selecting rules,based on the research on 2QD30A17K-I,the representative of high voltage and high power driver.

关键词： IGBTs 电力电子技术 MOSFET 晶体管

Study on the causes of local recurrence of rectal cancer after curative resection: analysis of 213 cases

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World Journal of Gastroenterology 1998年第6期4卷 72-74页

作者： YUAN Hong yin, li yan, yanG Guo liang, BEI De Jiao and WANG Kun

AIM To study the local recurrent rate and the causes of rectal cancer after surgery. METHODS The clinicopathological data of 213 rectal cancer patients and the follow up information were analyzed. The overall recurrent rate and the recurrent rates from different surgical appreaches were calculated. The main causes of recurrence were investigated. RESULTS Among the 213 cases, 73 (34 27%) had local recurrence. The recurrent time ranged from 3 months to 62 months after the first operation. Most of the recurrence ( 65/73 , 89 04%) occurred within 3 years after operation. CONCLUSION Local recurrence had no significant correlation with surgical methods or pathological types, but closely related to Dukes′ stages, location of primary tumors and the length of the distal rectum resected. Early resection and a wide tumor free resection margin are key factors to prevent local recurrence.

关键词： rectal neoplasms/surgery Rectal neoplasms/pathology Neoplasm recurrent, local

Ultrasonic elastography in clinical quantitative assessment of fatty liver

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World Journal of Gastroenterology 2010年第37期16卷 4733-4737页

作者： yin-yan li Xue-Mei Wang Yi-Xia Zhang Guo-Cheng Ou Department of Ultrasonic Diagnosis the First Af��liated Hospital of China Medical UniversityShenyang 110001Liaoning ProvinceChina

AIM: To investigate the clinical application of ultrasonic elastography in quantitative assessment of fatty liver grading. METHODS: A total of 105 patients with fatty liver were divided into mild group (n = 46), moderate group (n = 39), and severe group (n = 20). Forty-five healthy individuals served as a normal control group. All patients who underwent routine ultrasound scan and further ultrasonic elastography were evaluated accordingly to the evaluation standards for ultrasonic elastography. The ratio of surface areas of blue region/total surface area in the desired region was measured. RESULTS: Ultrasonic elastography technique, in comparison to traditional ultrasound, had a rather high consistence in grading of fatty liver [κ value = (95.3%63.6%)/(1%-63.6%) = 0.87, P = 0.001]. The score of ultrasonic elastography increased with the severity of fatty liver with a sensitivity of 97.14% and a specificity of 91.11%. A significant difference was found in the ratio of surface areas of blue regions between different groups (P liver.

关键词： Transient elastography Ultrasonic elastography evaluation Fatty liver Quantitative diagnosis Grading of fatty liver

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