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The Epitope Study on the SARS-CoV Nucleocapsid Protein

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Genomics, Proteomics & Bioinformatics 2003年第3期1卷 198-206页

作者： Shuting Li, Liang Lin, Hao Wang, Jianning yin, Yan ren, zhe Zhao, Jie Wen, Cuiqi Zhou, Xumin Zhang, Xiaolei Li, Jingqiang Wang, zhengfeng Zhou, Jinxiu Liu, Jianmin Shao, Tingting Lei, Jianqiu Fang, Ningzhi Xu, and Siqi LiuBeijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China & Beijing Proteomics Institute, Beijing 101300, China Beijing Genomics Institute Chinese Academy of Sciences Beijing 101300 China.

The nucleocapsid protein (N protein) has been found to be an antigenic protein in a number of coronaviruses. Whether the N protein in severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is antigenic remains to be elucidated. Using Western blot and Enzyme-linked Immunosorbent Assay (ELISA), the recombinant N proteins and the synthesized peptides derived from the N protein were screened in sera from SARS patients. All patient sera in this study displayed strong positive immunoreactivities against the recombinant N proteins, whereas normal sera gave negative immunoresponses to these proteins, indicating that the N protein of SARS-CoV is an antigenic protein. Furthermore, the epitope sites in the N protein were determined by competition experiments, in which the recombinant proteins or the synthesized peptides competed against the SARS-CoV proteins to bind to the antibodies raised in SARS sera. One epitope site located at the C-terminus was confirmed as the most antigenic region in this protein. A detailed screening of peptide with ELISA demonstrated that the amino sequence from Codons 371 to 407 was the epitope site at the C-terminus of the N protein. Understanding of the epitope sites could be very significant for developing an effective diagnostic approach to SARS.

关键词： SARS coronavirus nucleocapsid protein antigenicity epitope

Correlation of magnetic resonance imaging quantitative parameters and apparent diffusion coefficient value with pathological breast cancer

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World Journal of Clinical Cases 2022年第21期10卷 7333-7340页

作者： zhe Wang Guan-ying ren Qian yin Qian Wang Department of Medical Imaging The No.2 Hospital of BaodingBaoding 071051Hebei ProvinceChina Department of Medical Oncology Affiliated Hospital of Hebei UniversityHebei Key Laboratory of Cancer Radiotherapy and ChemotherapyBaoding 071000Hebei ProvinceChina

BACKGROUND China ranks 120th worldwide for the incidence of breast cancer and 163rd for *** screening,diagnosis,and timely determination of the optimal treatment plan can help ensure clinical efficacy and *** To investigate the relationship between quantitative magnetic resonance imaging parameters,apparent diffusion coefficient value,pathological immunohistochemical status,and patient *** A total of 108 patients with breast cancer(breast cancer group)and 110 patients with benign breast tumors(benign group)confirmed by pathological examination at our Hospital from September 2013 to August 2016 were *** patients had undergone preoperative magnetic resonance imaging(MRI)examinations,and the quantitative parameters of MRI and apparent diffusion coefficient(ADC)values for the two groups were *** MRI quantitative parameters and ADC values of patients with different estrogen receptor(ER),progesterone receptor,and human epidermal growth factor receptor-2 expression were statistically *** relationship between the quantitative parameters of MRI and ADC values and patient recurrence was analyzed using receiver operating *** The measured values of the quantitative parameters of MRI-Ktrans,Kep,and Ve in the breast cancer group were higher than those in the benign group;the ADC value in the breast cancer group was lower than that in the benign group,and the difference was statistically significant(Prence group were significantly lower than those of the non-recurrence group,and the difference was statistically significant(P<0.05).CONCLUSION MRI quantitative parameters and ADC are related to the expression of breast c

关键词： Magnetic resonance Apparent diffusion coefficient Breast cancer Immunohistochemistry Prognosis

Altered metabolic state impedes limb regeneration in salamanders

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Zoological Research 2021年第6期42卷 772-782页

作者： zhe-Lun Peng Bin-Xu yin Rui-Min ren yin-Long Liao Hao Cai Heng Wang College of Animal Sciences and Technology Huazhong Agricultural UniversityWuhanHubei 430070China Shenzhen Institute of Nutrition and Health Huazhong Agricultural UniversityShenzhenGuangdong 518120China Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenGuangdong 518120China

Salamanders are unique among tetrapods in their ability to regenerate the limbs throughout *** other poikilothermic amphibians,salamanders also show a remarkable capacity to survive long periods of *** the physiological reserves necessary for tissue regeneration are preserved or sacrificed in starved salamanders is *** the current study,we maintained Iberian ribbed newts(Pleurodeles waltl)under extreme physiological stress to assess the extent of regeneration and identify the molecular and cellular changes that may occur under such *** 19 months of complete food deprivation,the animals exhibited extensive morphological and physiological adaptations but remained behaviorally active and *** was elevated in different tissues and the transformed gut microbiota indicated remodeling of the intestinal tract related to *** limb amputation in animals starved for 21 months,regeneration proceeded with progenitor cell proliferation and migration,leading to limb blastema ***,limb outgrowth and patterning were substantially *** of autophagy inhibited cell proliferation and blastema formation in starved animals,but not in fed ***,tissue autophagy and the regenerative response were tightly coupled only when animals were under *** results demonstrate that under adverse conditions,salamanders can exploit alternative strategies to secure blastema formation for limb regeneration.

关键词： Starvation Stress Autophagy Regeneration Salamander

Conductive,Stretchable,and Self-healing Ionic Gel Based on Dynamic Covalent Bonds and Electrostatic Interaction

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Chinese Journal of Polymer Science 2019年第11期37卷 1053-1059页

作者： Yi Sun Yong-Yuan ren Qi Li Rong-Wei Shi yin Hu Jiang-Na Guo zhe Sun Feng Yan Department of Polymer Science and Engineering College of Chemistry Chemical Engineering and Materials Science Soochow University

Integrating multiple functions into one gel that can be widely applied to electronic devices as well as chemical and biomedical engineering remains a big ***,a multifunctional ionic liquid/dynamic covalent bonds (ionic/DCB) type gel was designed and synthesized via one-pot *** the assistance of electrostatic interaction provided by the imidazolium cations of IL and the reversible DCB of boronic ester,as-prepared ionic/DCB gel showed good stretchable properties and high ionic conductivity at ambient *** addition,the electrostatic interaction between imidazolium cations and sulfonate anions and the reversible DCB led to enhanced chain mobility and thereby excellent self-healing ***,sulfonate anions in ionic/DCB gel could alleviate the migration of electronegative polysulfide and promote the transportation of electropositive lithium ion in lithium-sulfur battery ***,this work provides a new insight to promote the current research on self-healing gels,hopefully expanding their applications in electronic devices.

关键词： Ionic gel Conductivity Self-healing Dynamic covalent bonds Lithium-sulfur battery

FOXO1 Alleviates Liver Ischemia-reperfusion Injury by Regulating the Th17/Treg Ratio through the AKT/Stat3/FOXO1 Pathway

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Journal of Clinical and Translational Hepatology 2022年第6期10卷 1138-1147页

作者： Hao-zhen ren Sen-zhe Xia Xue-Qian Qin An-yin Hu Jing-Lin Wang Department of Hepatobiliary Surgery Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjingJi-angsuChina Department of Hepatobiliary Surgery Nanjing Drum Tower Hospital Clinical College of Jiangsu UniversityNanjingJiangsuChina Department of Hepatobiliary Surgery Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western MedicineNanjing University of Chinese MedicineNanjingJiangsuChina

Background and Aims:Hepatic ischemic reperfusion in-jury(IRI)occurring during surgery seriously affects patient *** specific mechanism of IRI has not been fully *** study aim was to explore the changes of in-flammatory environment,and the relationship of the Th17/Treg cell ratio and FOXO1 expression in hepatic ***:Liver samples at different ischemic times were collected from patients and *** expression of inflammatory markers and FOXO1 in the liver was detected by western blotting and *** changes of liver lymphocytes were analyzed by flow *** AKT/Stat3/FOXO1 pathway was veri-fied by targeting AKT with *** role of FOXO1 in liver inflammation and changes in lymphocyte phenotype was confirmed by upregulating FOXO1 with ***-sults:Prolonged ischemic time aggravates liver injury in both humans and mouse models of hepatic ***-stress caused Th17/Treg imbalance and FOXO1 down-regulation by activat-ing the AKT/Stat3/FOXO1 signaling *** of FOXO1 reversed the Th17/Treg cytokine imbalance and altered the inflammation environment in the ***:Liver IRI induced Th17/Treg *** of FOXO1 re-versed the imbalance and alleviated liver inflammation.

关键词： Liver ischemia-reperfusion injury Inflammatory factors Th17 Treg AKT/Stat3/FOXO1 pathway

Adaptive Dynamic Surface Control for Integrated Missile Guidance and Autopilot

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International Journal of Automation and computing 2011年第1期8卷 122-127页

作者： Ming-zhe Hou Guang-ren Duan Center for Control Theory and Guidance Technology Harbin Institute of Technology Harbin 150001 PRC

Integrated guidance and control for homing missiles utilizing adaptive dynamic surface control approach is considered based on the three channels independence design idea. A time-varying integrated guidance and control model with unmatched uncertainties is first formulated for the pitch channel, and an adaptive dynamic surface control algorithm is further developed to deal with these unmatched uncertainties. It is proved that the proposed feedback controller can ensure not only the accuracy of target interception, but also the stability of the missile dynamics. Then, the same control approach is further applied to the control design of the yaw and roll channels. The 6-degree-of-freedom （6-DOF） nonlinear missile simulation results demonstrate the feasibility and advantage of the proposed integrated guidance and control design scheme.

关键词： Integrated guidance and control unmatched uncertainty adaptive control dynamic surface control homing missiles.

Three positive charge nonapoptotic-induced photosensitizer with excellent water solubility for tumor therapy

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Chinese Chemical Letters 2024年第8期35卷 205-210页

作者： zhe Li Ping-Zhao Liang Li Xu Fei-Yu Yang Tian-Bing ren Lin Yuan Xia yin Xiao-Bing Zhang State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringHunan UniversityChangsha 410082China

Photodynamic therapy(PDT)has emerged as a significant cancer therapy ***,cation-based organic small molecule aggregation-induced emission(AIE)photosensitizers(PSs)attract the wide atten-tion of many scientists,due to improved reactive oxygen species(ROS)production after ***,such PSs tend to localize only the mitochondria,limiting the death way of tumor cells(usu-ally apoptosis)during PDT process,which may affect the therapeutic effect under some ***,we designed a novel water-soluble three positive charge PS,TPAN-18F,which could be distributed uniformly in cell cytoplasm and had distribution in different sub-organelles(mitochondria,endoplasmic reticulum,lysosome).The experimental results showed that TPAN-18F-based PDT process can not only disrupt mitochondrial functions(reducing ATP production and destroying mitochondrial membrane po-tential),but also elevate the intracellular lipid peroxides(LPOs)level,which evoke the non-apoptotic death manner of tumor ***,in vivo studies showed that TPAN-18F-based PDT could effectively inhibit tumor ***,we believe that the construction of TPAN-18F is suggestive for tumor non-apoptotic therapy.

关键词： Water-soluble Three positive charge Photosensitizer Nonapoptotic Tumor therapy

New Cassane Diterpenoids from the Seed Kernels of Caesalpinia cucullata, Exhibit Anti-inflammatory Effect in vitro by Inhibiting iNOS Enzymatic Activity

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Chinese Journal of Chemistry 2021年第6期39卷 1625-1634页

作者： Miao Wang Yi-ren Yang Yan yin Kai-Ru Song Li-Ping Long Xian-zhe Li Bei Zhou Hui-Yuan Gao School of Traditional Chinese Moterio Medico Shenyang Pharmaceutical UniversityShenyangLiaoning 110016China Key Laboratory of Structure-Based Drug Design&Discovery of Ministry of Education Shenyang Pharmoceuticol UniversityShenyangLiaoning 110016China

Inflammation is a complex biological response to *** cassane diterpenoids from Caesalpinia genus exhibit significant anti-inflammation *** new cassane diterpenoids(1-8)along with seven known ones(9-15)were obtained from the seed kernels of Caesalpinia cucullata *** is the first report on chemical investigation of the seed kernels of ***,and the cassane diterpenes were found in this plant for the first *** structures were elucidated based on the extensive spectroscopic analyses,and the absolute configurations were identified by ECD calculation and X-ray *** compounds were evaluated for their anti-inflammation activity by inhibiting NO production in LPS-induced RAW 264.7 *** 1-2 and 9-11 exhibited effective inhibitory activity with inhibition rate more than 50%.The iNOS enzyme activity and molecular docking experiments were performed to explore the preliminary ***,a potential anti-inflammatory mechanism revealed that the bioactive cassane inhibited overproduction of NO by targeting key residues in the iNOS active cavity to reduce iNOS enzymatic activation.

关键词： Caesalpinia cucullata Roxb. Cassane diterpenoids Structure elucidation Biological activity iNOS enzyme activity

Complete oxidation of ethanol over vanadium based catalysts

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Journal of Natural Gas Chemistry 2009年第3期18卷 379-382页

作者： zhe Li Xia An Panjie ren Wei Huang Kechang Xie Key Laboratory of Coal Science and Technology of Ministry of Education Taiyuan University of Technology Taiyuan 030024 China

V2O5/γ-Al2O3-TiO2 catalysts were prepared by the mixing sol-gel and co-impregnation method. The performance of the catalysts for complete oxidation of ethanol was evaluated in a conventional fixed-bed quartz reactor. And the effects of support, preparation methods and vanadium content have been investigated. The results showed that 5% V2O5 catalyst supported on γ-Al2O3-TiO2 possessed the best ethanol conversion under the considered temperature. This may be ascribed to the highly dispersible active component, mutual function between the active component and the carriers. The nature of the best performance for 5%V/γ-Al2O3-TiO2 catalyst may be related to the high V4＋ amounts on the surface. And the surface V4＋ species may play an important role in the formation of active site for the total ethanol oxidation.

关键词： ethanol γ-Al2O3-TiO2 V2O5 catalytic oxidation