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DNA vaccine prime and replicating vaccinia vaccine boost induce robust humoral and cellular immune responses against MERS-CoV in mice

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Virologica Sinica 2024年第3期39卷 490-500页

作者： Xiuli Shen Shuhui Wang Yanling Hao Yuyu Fu Li Ren Dan Li Wenqi Tang Jing Li Ran chen Meiling Zhu Shuo Wang Ying Liu yiming Shao National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases National Center for AIDS/STD Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Changping Laboratory Yard 28Science Park RoadChangping DistrictBeijing 102206China State Key Laboratory for Diagnosis and Treatment of Infectious Diseases National Clinical Research Center for Infectious DiseasesCollaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesThe First Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310003China

As of December 2022,2603 laboratory-identified Middle East respiratory syndrome coronavirus(MERS-CoV)infections and 935 associated deaths,with a mortality rate of 36%,had been reported to the World Health Organization(WHO).However,there are still no vaccines for MERS-CoV,which makes the prevention and control of MERS-CoV *** this study,we generated two DNA vaccine candidates by integrating MERS-CoV Spike(S)gene into a replicating Vaccinia Tian Tan(VTT)*** to homologous immunization with either vaccine,mice immunized with DNA vaccine prime and VTT vaccine boost exhibited much stronger and durable humoral and cellular immune *** immunized mice produced robust binding antibodies and broad neutralizing antibodies against the EMC2012,England1 and KNIH strains of ***-Boost immunization also induced strong MERS-S specific T cells responses,with high memory and poly-functional(CD107a-IFN-γ-TNF-α)effector CD8t T *** conclusion,the research demonstrated that DNA-Prime/VTT-Boost strategy could elicit robust and balanced humoral and cellular immune responses against *** study not only provides a promising set of MERS-CoV vaccine candidates,but also proposes a heterologous sequential immunization strategy worthy of further development.

关键词： MERS-CoV VTT vaccine DNA vaccine Humoral and cellular immune responses Prime/boost strategy

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Thyroid Hormone Biomonitoring:A Review on Their Metabolism and Machine-Learning Based Analysis on Effects of Endocrine Disrupting Chemicals

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Environment & Health 2024年第6期2卷 350-372页

作者： Shijie chen Mengjie Yu yiming Yao Yongcheng Li Ana He Zijun Zhou Liyang Pan Nan Xiao Haining Luo Hongwen Sun MOE Key Laboratory of Pollution Processes and Environmental Criteria College of Environmental Science and EngineeringNankai UniversityTianjin 300071China Tianjin Tianshi College Tianjin 301700China Dalian Center for Disease Control and Prevention DalianLiaoning Province 116000China Department of Center for Reproductive Medicine Tianjin Central Hospital Of Gynecology Obstetrics/Tianjin Key Laboratory Of Human Development And Reproductive RegulationTianjin 300052China

The thyroid is an essential endocrine organ in human body,and thyroid hormones(THs)are pivotal signaling molecules and mediators in various physiological ***,particularly in their free form,play a critical role in regulating body temperature and in the metabolism of lipid and glucose,making the maintenance of TH levels crucial for human *** undergo a series of metabolic processes,producing TH metabolites(THMs).THMs are significant in endocrine regulation,such as 3,5-diiothyronine(3,5-T2)and 3-iodothyronamine(3-T1AM),which exhibit activities akin to *** production and distribution of THMs are intricately linked to the function of specific organs and tissues,highlighting the need for advanced research into the determination and mechanisms of THMs in *** to endocrine disrupting chemicals(EDCs)can significantly affect the levels of thyroid stimulating hormone(TSH)and *** review utilizes machine learning to analyze epidemiological data,identifying potential EDCs that pose risks of hyperthyroidism and ***,it delves into the toxicological mechanisms of these EDCs,examining their effects on TH production,binding processes,related proteins,and metabolic *** approach effectively bridges the gap between epidemiological studies and toxicological researches,laying the groundwork for future research *** integrating epidemiological studies with machine learning,this review offers insightful perspectives on the potential risks associated with chemical exposure and underscores the necessity for further research in understanding the impact of EDCs on TH metabolism and TH-related health effects.

关键词： Thyroid stimulating hormone Emerging contaminants Data mining Thyroxine 3,3′,5-Triiodothyronine Homeostasis

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Recent advances in chemical protein synthesis:method developments and biological applications

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Science China Chemistry 2024年第4期67卷 1060-1096页

作者： Suwei Dong Ji-Shen Zheng yiming Li Huan Wang Gong chen Yongxiang chen Gemin Fang Jun Guo Chunmao He Honggang Hu Xuechen Li Yanmei Li Zigang Li Man Pan Shan Tang Changlin Tian Ping Wang Bian Wu Chuanliu Wu Junfeng Zhao Lei Liu State Key Laboratory of Natural and Biomimetic Drugs and School of Pharmaceutical SciencesPeking UniversityBeijing 100191China Division of Life Sciences and Medicine University of Science and Technology of ChinaHefei 230027China School of Food and Biological Engineering Engineering Research Center of Bio-processMinistry of EducationHefei University of TechnologyHefei 230009China School of Chemistry and Chemical Engineering Nanjing UniversityNanjing 210023China State Key Laboratory of Elemento-Organic Chemistry Nankai UniversityTianjin 300071China Department of Chemistry Tsinghua UniversityBeijing 100084China Institute of Health Sciences Anhui UniversityHefei 230601China College of Chemistry Central China Normal UniversityWuhan 430079China School of Chemistry and Chemical Engineering South China University of TechnologyGuangzhou 510640China School of Medicine or Institute of Translational Medicine Shanghai UniversityShanghai 200444China Department of Chemistry The University of Hong KongHong Kong 999077China Shenzhen Graduate School Peking UniversityShenzhen 518055China School of Chemistry and Chemical Engineering Shanghai Jiao Tong UniversityShanghai 200240China School of Life Sciences University of Science and Technology of ChinaHefei 230026China Department of Chemical Biology Shanghai Jiao Tong UniversityShanghai 200240China Institute of Microbiology Chinese Academy of SciencesBeijing 100101China Department of Chemistry Xiamen UniversityXiamen 361005China School of Pharmaceutical Science Guangzhou Medical UniversityGuangzhou 511436China

The central dogma of modern biology underscores the pivotal roles proteins play in diverse biological processes,the study of which necessitates advanced methods to produce proteins with precision and *** protein synthesis,a powerful approach utilizing chemical reactions for the de novo construction of structurally accurate proteins,has emerged as a transformative tool for studying proteins and generating protein derivatives/mimics inaccessible by natural biological machinery,including post-translationally modified proteins,proteins comprised of unnatural amino acids,as well as mirror-image *** review summarizes recent strides in synthetic method developments for chemical protein synthesis,including innovative techniques in solid-phase peptide synthesis,the challenges presented by difficult sequences in either synthesis or folding and the exploration of novel ligation reactions using both chemical and enzymatic ***,the review also delves into newly developed protocols for site-selective protein modifications and the generation of stapled or macrocyclized peptides/miniproteins,highlighting the power of chemical methods to make structurally diverse *** applications of synthetic proteins in investigating post-translational modifications(phosphorylation,lipidation,glycosylation,ubiquitination,etc.),mirror-image biological processes and drug development are further ***,these topics provide a comprehensive overview of the current landscape of chemical protein synthesis.

关键词： chemical protein synthesis solid-phase peptide synthesis ligation reactions post-translational modifications mirror-image proteins peptide drugs

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Correlation between rate-pressure product or pressure-rate quotient and urinary albumin-creatinine ratio in the Chinese older population:The REACTION study

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Chinese Medical Journal 2024年第2期137卷 238-240页

作者： Linghuan Wang Peixin Wu Kang chen Binqi Li Jie Wang yiming Mu Department of Endocrinology The First Medical CenterChinese PLA General HospitalNational Key Laboratory of Kidney DiseasesBeijing 100853China Department of Medicine School Nankai UniversityTianjin 300071China Department of Endocrinology Beijing Chao-Yang HospitalBeijing 100020China

To the Editor:Chronic kidney disease(CKD)is a critical health *** elevated urinary albumin-creatinine ratio(UACR)is a marker of impaired renal function and is a key risk factor for cardiovascular *** rate-pressure product(RPP)[1]and pressure rate quotient(PRQ)are widely used as methods to quantify cardiac load and hemodynamic response to exercise in clinical ***,to better predict CKD,it is of great signicance to explore the relationships between RPP or PRQ and UACR.

关键词： urinary impaired creatinine

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Aberration correction for deformable-mirror-based remote focusing enables high-accuracy whole-cell super-resolution imaging

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Photonics Research 2024年第4期12卷 821-832页

作者： WEI SHI YINGCHUAN HE JIANLIN WANG LULU ZHOU JIANWEI chen LIWEI ZHOU ZEYU XI ZHEN WANG KE FANG yiming LI Department of Biomedical Engineering Southern University of Science and TechnologyShenzhen 518055China

Single-molecule localization microscopy(SMLM)enables three-dimensional(3D)investigation of nanoscale structures in biological samples,offering unique insights into their ***,traditional 3D super-resolution microscopy using high numerical aperture(NA)objectives is limited by imaging depth of field(DOF),restricting their practical application to relatively thin biological ***,we developed a unified solution for thick sample super-resolution imaging using a deformable mirror(DM)which served for fast remote focusing,optimized point spread function(PSF)engineering,and accurate aberration *** effectively correcting the system aberrations introduced during remote focusing and sample aberrations at different imaging depths,we achieved high-accuracy,large DOF imaging(~8μm)of the whole-cell organelles[i.e.,nuclear pore complex(NPC),microtubules,and mitochondria]with a nearly uniform resolution of approximately 35 nm across the entire cellular volume.

关键词： enable mirror resolution

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Plant genomic resources at National Genomics Data Center:assisting in data-driven breeding applications

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aBIOTECH 2024年第1期5卷 94-106页

作者： Dongmei Tian Tianyi Xu Hailong Kang Hong Luo Yanqing Wang Meili chen Rujiao Li Lina Ma Zhonghuang Wang Lili Hao Bixia Tang Dong Zou Jingfa Xiao Wenming Zhao yiming Bao Zhang Zhang Shuhui Song National Genomics Data Center Beijing Institute of GenomicsChinese Academy of Sciences&China National Center for BioinformationBeijing 100101China CAS Key Laboratory of Genome Sciences and Information Beijing Institute of GenomicsChinese Academy of Sciences&China National Center for BioinformationBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China

Genomic data serve as an invaluable resource for unraveling the intricacies of the higher plant systems,including the constituent elements within and among *** various efforts in genomic data archiving,integrative analysis and value-added curation,the National Genomics Data Center(NGDC),which is a part of the China National Center for Bioinformation(CNCB),has successfully established and currently maintains a vast amount of database *** dedicated initiative of the NGDC facilitates a data-rich ecosystem that greatly strengthens and supports genomic research ***,we present a comprehensive overview of central repositories dedicated to archiving,presenting,and sharing plant omics data,introduce knowledgebases focused on variants or gene-based functional insights,highlight species-specific multiple omics database resources,and briefly review the online application *** intend that this review can be used as a guide map for plant researchers wishing to select effective data resources from the NGDC for their specific areas of study.

关键词： Plant-omics data Data repositories Data integration Knowledgebase Plant genomics

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生态涵养区农民人均纯收入格局时空演变分析——以北京市怀柔区为例

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林业经济 2018年第3期40卷 107-112页

作者：谢一鸣陈怩张彩虹蔡飞赵陟峰贾子靖北京林业大学经济管理学院北京100083 北京北林先进生态环保技术研究院有限公司北京100083

文章利用1996-2015年北京市怀柔区农民人均纯收入数据，采用数理统计和空间统计方法从时间、空间以及时空的角度对怀柔区14个乡镇的农民收入水平的区域差异和格局演变过程u进行分析，逐类分析冷、热点区农民收入水平的特点，探索生态涵... 详细信息

文章利用1996-2015年北京市怀柔区农民人均纯收入数据，采用数理统计和空间统计方法从时间、空间以及时空的角度对怀柔区14个乡镇的农民收入水平的区域差异和格局演变过程u进行分析，逐类分析冷、热点区农民收入水平的特点，探索生态涵养区农民收入的长期规律性。结果表明：怀柔区农民收入水平持续增长，乡镇问的农民收入差距也在逐年提升，但乡镇间农民收入的相对差异以及两极分化现象均呈现减弱态势。各乡镇相似的农民收入水平地区在空间上集聚分布，存在着空间正向自相关的现象，并呈现出从南向北梯度递减的空间格局态势。结合怀柔区生态涵养区的林业资源条件提出不同区域的农民收入增收的有效途径。

关键词：怀柔生态涵养区农民收入区域格局时空演变

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探寻式搜索中用户满意度状态识别与演化规律研究

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数据分析与知识发现 2024年第1期8卷 90-103页

作者：赵一鸣陈湛张帆武汉大学信息资源研究中心武汉430072 武汉大学信息管理学院武汉430072 武汉大学大数据研究院武汉430072 武汉大学图书情报国家级实验教学示范中心武汉430072 武汉大学科教管理与评价中心武汉430072

【目的】识别探寻式搜索中的用户满意度状态,揭示用户满意度与用户查询式重构模式之间的相互作用关系和动态演化规律。【方法】利用用户查询、时序等特征,基于4种有监督学习算法进行用户满意度预测;通过挖掘用户满意度与查询式重构模式... 详细信息

【目的】识别探寻式搜索中的用户满意度状态,揭示用户满意度与用户查询式重构模式之间的相互作用关系和动态演化规律。【方法】利用用户查询、时序等特征,基于4种有监督学习算法进行用户满意度预测;通过挖掘用户满意度与查询式重构模式之间的相互影响规律,指导探寻式搜索智能辅助中的查询式重构推荐策略。【结果】在开放基准数据集上,所构建的满意度预测模型的预测准确率最高达到74%,优于已有基线模型;同时,相关性分析的结果表明用户满意度与查询式重构模式之间的关联关系显著。【局限】用户满意度仅代表搜索状态的一种视角,未来需要针对探寻式搜索中的用户状态构建完善统一的描述和分类体系。【结论】利用探寻式搜索中的用户搜索行为,优化了模型特征,进一步提升用户满意度预测模型的性能,并结合用户满意度演化规律,为探寻式搜索中的智能搜索辅助策略提供了有效的理论支撑。

关键词：探寻式搜索用户满意度用户满意度预测查询式重构

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Imaging momentum-space Cooper pair formation and its competition with the charge density wave gap in a kagome superconductor

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Science China(Physics,Mechanics & Astronomy) 2024年第7期67卷 142-150页

作者： yiming Sun Yubing Tu Yang Luo Shuikang Yu Hongyu Li Yunmei Zhang Ping Wu Zhuying Wang Fan Zhang Wanru Ma Zuowei Liang Jianjun Ying Tao Wu Ziji Xiang Junfeng He Lei Shan Zhenyu Wang Xianhui chen Department of Physics CAS Key Laboratory of Strongly Coupled Quantum Matter Physicsand Hefei National LaboratoryUniversity of Science and Technology of ChinaHefei 230026China Information Materials and Intelligent Sensing Laboratory of Anhui Province Institutes of Physical Science and Information TechnologyAnhui UniversityHefei 230601China Collaborative Innovation Center of Advanced Microstructures Nanjing 210093China

The superconducting ground state of kagome metals AV_(3)Sb_(5)(where A stands for K,Rb,or Cs)emerges from an exotic charge density wave(CDW)state that potentially breaks both rotational and time reversal ***,the specifics of the Cooper pairing mechanism,and the nature of the interplay between these two states remain elusive,largely due to the lack of momentum-space(k-space)superconducting energy gap *** implementing Bogoliubov quasiparticle interference(B QPI)imaging,we obtain k-space information on the multiband superconducting gap structureΔ_(SC)^(i)(k)in pristine CsV_(3)Sb_(5).We show that the estimated energy gap on the vanadium d_(xy/x^(2)-y^(2))orbital is anisotropic but nodeless,with a minimal value located near the M ***,a comparison ofΔ_(SC)^(i)(k)with the CDW gapΔ_(CDW)^(i)(k)obtained by angle-re solved photoemission spectro scopy(ARPES)reveals direct k-space competition between the se two order parameters,i.e.,the opening of a large(small)CDW gap at a given momentum corresponds to a small(large)superconducting *** the long-range CDW order is suppressed by replacing vanadium with titanium,we find a nearly isotropic energy gap on both the V and Sb *** information will be critical for identifying the microscopic pairing mechanism and its interplay with intertwined electro nic orders in this kagome superconductor family.

关键词： kagome lattice charge density wave superconducting energy gap scanning tunneling microscopy

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Homogeneous permeation and oriented crystallization in nanostructured mesopores for efficient and stable printable mesoscopic perovskite solar cells

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Science China Chemistry 2024年第11期67卷 3688-3696页

作者： Guodong Zhang Yanjie cheng Tingting Niu Ziwei Zheng Zongwei Li Junwei Xiang Qiaojiao Gao Minghao Xia Lijuan Guo yiming Liu Mengru Zhang Yiran Tao Xueqin Ran Mingjie Li Guichuan Xing Yingdong Xia Lingfeng Chao Anyi Mei Hongwei Han Yonghua chen Key Laboratory of Flexible Electronics (KLoFE) and Institute of Advanced Materials (IAM) School of Flexible Electronics (Future Technologies) Nanjing Tech University Michael Gr?tzel Center for Mesoscopic Solar Cells Wuhan National Laboratory for Optoelectronics Key Laboratory of Materials Chemistry for Energy Conversion and Storage of Ministry of Education Huazhong University of Science and Technology Department of Physics The Chinese University of Hong Kong Department of Applied Physics The Hong Kong Polytechnic University Shenzhen Research Institute The Hong Kong Polytechnic University Joint Key Laboratory of the Ministry of Education Institute of Applied Physics and Materials Engineering University of Macau

The low-cost and scalable printable mesoporous perovskite solar cells(p-MPSCs) face significant challenges in regulating perovskite crystal growth due to their nanoscale mesoporous scaffold structure, which limits the improvement of device power conversion efficiency(PCE). In particular, the most commonly used solvents, N,N-dimethylformamide(DMF) and dimethyl sulfoxide(DMSO), have a single chemical interaction with the precursor components and high volatility, which is insufficient to self-regulate the perovskite crystallization process, leading to explosive nucleation and limited growth within mesoporous scaffolds. Here, we report a mixed solvent system composed of methylamine formaldehyde(MAFa)-based ionic liquid and acetonitrile(ACN) with the strong C=O–Pb coordination and N–H···I hydrogen bonding with perovskite components. We found that the mixed solvent system is beneficial for the precursor solution to homogeneously penetrate into the mesoporous scaffold,and the strong C=O–Pb coordination and N–H···I hydrogen bonding interaction can promote the oriented growth of perovskite crystals. This synergistic effect increased the PCE of the p-MPSCs from 17.50% to 19.21%, which is one of the highest records for p-MPSC in recent years. Additionally, the devices exhibit positive environmental stability, retaining over 90% of the original PCE after 1,200 h of aging under AM 1.5 illumination conditions at 55 °C and 55% humidity.

关键词： printable mesoscopic perovskite solar cells ionic liquid crytallization control hight efficiency and stability

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