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Observations on a magnetotactic bacteria-grazing ciliate in sediment from the intertidal zone of Huiquan Bay,China

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Journal of Oceanology and Limnology 2021年第6期39卷 2053-2062页

作者： Si CHEN Kaixuan CUI Wenyan ZHANG yicong zhao Tian XIAO Hongmiao PAN Wuchang ZHANG Long-Fei WU CAS Key Laboratory of Marine Ecology and Environmental Sciences Institute of OceanologyChinese Academy of SciencesQingdao 266071China University of Chinese Academy of Sciences Beijing 100049China Laboratory for Marine Ecology and Environmental Science Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China International Associated Laboratory of Evolution and Development of Magnetotactic Multicellular Organisms(LIA-MagMC) CNRS-CASQingdao 266071China Aix Marseille Univ CNRSLCBIM2BIMMMarseille 13009France

Magnetotactic bacteria(MTB)are a group of prokaryotes having the ability to orient and swim along geomagnetic field lines because they contain intracellular magnetosomes that are synthesized through a biomineralization *** have recently also been found in unicellular eukaryotes,which are referred to as magnetically responsive protists(MRPs).The magnetosomes have three origins in *** this study,we characterized a MTB-grazing ciliated MRP that was magnetically collected from intertidal sediment of Huiquan Bay,Qingdao,*** on 18S rRNA gene sequence analysis,the ciliated MRP was tentatively identified as Uronemella parafi lificum *** transmission electron microscopy,we observed that magnetosomes having 2-3 shapes were randomly distributed within this *** X-ray spectroscopy and high-resolution transmission electron microscopy images of the magnetosomes were consistent with them being composed of *** having the same shape and mineral composition were also detected in MTB that occurred in the same environment as the ciliated *** analysis showed that the size and shape of the magnetosomes in the ciliated MRP were similar to those in *** results suggest that this ciliated MRP can graze,ingest,and digest various types of *** is certainly worth noting that this is the first record of MRPs in Asian aquatic sediment and suggesting they might be widely *** results also support the assertion that MRPs probably contribute to the ecological cycles of iron,and expand possibilities for research into the mechanism of magnetoreception in eukaryotes.

关键词： magnetically responsive protist ciliate magnetotactic bacteria magnetosome graze magnetoreception

去氢骆驼蓬碱治疗细粒棘球蚴病的作用机制研究

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中国药业 2024年第11期33卷 42-48页

作者：林玉霞巩月红赵一聪潘美驰王建华新疆医科大学药学院新疆乌鲁木齐830011 新疆医科大学第一附属医院新疆乌鲁木齐830054 新疆医科大学省部共建中亚高发病成因与防治国家重点实验室新疆乌鲁木齐830000

目的应用网络药理学方法预测去氢骆驼蓬碱(HM)治疗细粒棘球蚴病的作用机制。方法从中药系统药理学数据库(TCMSP)、瑞士靶点预测数据库(SwissTargetPrediction)、药物银行数据库(Drugbank)获取HM相关基因,在基因卡数据库(GeneCards)、D... 详细信息

目的应用网络药理学方法预测去氢骆驼蓬碱(HM)治疗细粒棘球蚴病的作用机制。方法从中药系统药理学数据库(TCMSP)、瑞士靶点预测数据库(SwissTargetPrediction)、药物银行数据库(Drugbank)获取HM相关基因,在基因卡数据库(GeneCards)、DISGENET数据库、DrugBank数据库获取细粒棘球蚴病的作用靶点,将HM的预测靶点和细粒棘球蚴病相关基因相互映射,得到HM作用细粒棘球蚴病的靶点基因,导入String数据库进行网络拓扑属性分析,筛选重要靶点蛋白,构建蛋白质-蛋白质互作(PPI)和成分-疾病-靶点网络。采用DAVID数据库进行基因本体论(GO)功能分析和Kyoto京都基因与基因组百科全书(KEGG)通路富集分析,筛选与DNA损伤相关的基因靶点。采用Autodock Vina软件及Pymol软件对相关靶点进行分子对接验证和展示。将活力不低于98%的细粒棘球蚴随机分为空白对照组、1%DMSO组、25μmol/L HM组、50μmol/L HM组、100μmol/L HM组,采用实时荧光定量逆转录聚合酶链式反应(qRTPCR)法、免疫印迹(Western blot)法体外验证HM干预细粒棘球蚴肿瘤蛋白P53(TP53)、拓扑异构酶Ⅰ(TopoⅠ)、染色体组蛋白H2A的变异体(H2AX)、共济失调毛细血管扩张突变基因(ATM)mRNA和蛋白表达水平。结果共获得HM靶点105个,细粒棘球蚴病相关基因88个,二者共有靶点为2个。HM干预细粒棘球蚴病的作用靶点主要有促分裂原活化蛋白激酶3(MAPK3)、MAPK1、TP53、热休克蛋白90α家族A类成员1(HSPAA1)、MAPK14、Jun,机制可能涉及MAPK信号通路、受体酪氨酸激酶(ERBB)信号通路、DNA损伤与DNA修复通路、细胞凋亡等过程。体外验证结果显示,与空白对照组相比,25μmol/L HM组、50μmol/L HM组、100μmol/L HM组的TP53,TopoⅠmRNA和蛋白表达均呈下调趋势(P <0.05),H2AX,ATM mRNA和蛋白表达均呈上调趋势(P <0.05)。结论 HM可通过多种生物学过程和相关信号途径治疗细粒棘球蚴病,HM参与细粒棘球蚴病DNA损伤与DNA修复信号通路的调控作用,TP53,TopoⅠ,H2AX,ATM均可能是其作用靶点。该研究为进一步阐明HM治疗细粒棘球蚴病的分子机制奠定了基础。

关键词：去氢骆驼蓬碱细粒棘球蚴病 DNA损伤与DNA修复实时荧光定量逆转录聚合酶链式反应法网络药理学作用机制

Characterization and diversity of magnetotactic bacteria from sediments of Caroline Seamount in the Western Pacific Ocean

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Journal of Oceanology and Limnology 2021年第6期39卷 2027-2043页

作者： Kaixuan CUI Wenyan ZHANG Jia LIU Cong XU yicong zhao Si CHEN Hongmiao PAN Tian XIAO Long-Fei WU CAS Key Laboratory of Marine Ecology and Environmental Sciences Institute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for Marine Ecology and Environmental Science Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China University of Chinese Academy of Sciences Beijing 100049China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China International Associated Laboratory of Evolution and Development of Magnetotactic Multicellular Organisms(LIA-MagMC) CNRS-CASQingdao 266071China Aix Marseille Univ CNRSLCBIM2BIMMMarseille 13009France National Oceanographic Center Qingdao 266071China

Magnetotactic bacteria(MTB)are a group of microorganisms capable of orientating and swimming along magnetic fields because they contain intracellular biomineralized magnetosomes composed of magnetite(Fe 3 O 4)or/and greigite(Fe_(3)S_(4)).They are ubiquitous in freshwater,brackish,and marine habitats,and are cosmopolitan in ***,knowledge of their occurrence and distribution in seamount ecosystems is *** investigated the diversity and distribution of MTB in the Caroline Seamount(CM4).The abundance of living MTB in 12 stations in depth varying from 90 to 1545 m was 1.1×10^(3)-43.7×10^(3) inds./dm *** diverse shapes of MTB observed,magnetotactic cocci were the dominant morphotype and could be categorized into two types:1)typical cocci that appeared to have peritrichous fl agella;and 2)those characterized by having a drop-shaped form and one bundle of fl agella located at the thin/narrow end of the *** electron microscopy(TEM)analysis revealed that the magnetosomes formed by those magnetotactic cocci are magnetite(Fe 3 O 4)with octahedral crystal habit.A total of 41 operational taxonomic units(OTUs)of putative MTB(2702 reads)were acquired from nine stations,based on high-throughput *** these,40 OTUs belonged to the Proteobacteria phylum and one belonged to the Nitrospirae *** found apparent connectivity between the MTB populations on the Caroline and Kexue(Science in Chinese)seamounts,although the diversity of MTB on Caroline was much richer than on the Kexue *** results imply that the unique topography of seamounts and other as-yet unclear environmental factors could lead to evolution of different fl agella arrangements in magnetotactic cocci,and the occurrence of octahedral magnetite magnetosomes.

关键词： magnetotactic bacteria Caroline Seamount abundance diversity magnetosome

去氢骆驼蓬碱对细粒棘球蚴中EgTP53蛋白生物信息学表达的影响

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新疆医科大学学报 2024年第2期47卷 268-274页

作者：林玉霞巩月红朱莉娜赵美玲潘美驰赵一聪王建华新疆医科大学药学院乌鲁木齐830011 新疆医科大学第一附属医院药学部省部共建中亚高发病成因与防治国家重点实验室乌鲁木齐830054

目的基于生物信息学表达分析去氢骆驼蓬碱对细粒棘球蚴中EgTP53蛋白(Echinococcus granulosus of Tumor protein p53,EgTP53)的影响。方法将EgTP53蛋白与去氢骆驼蓬碱进行分子对接,从美国国家生物技术信息中心数据库(National Center fo... 详细信息

目的基于生物信息学表达分析去氢骆驼蓬碱对细粒棘球蚴中EgTP53蛋白(Echinococcus granulosus of Tumor protein p53,EgTP53)的影响。方法将EgTP53蛋白与去氢骆驼蓬碱进行分子对接,从美国国家生物技术信息中心数据库(National Center for Biotechnology Information,NCBI)下载EgTP53的氨基酸序列,使用ProtParam蛋白分析工具进行EgTP53蛋白理化性质的预测,通过生信工具ProtScale进行疏水性的预测。使用SignalP5.0在线软件对信号肽进行预测,使用生物学在线软件工具TMHMM分析跨膜区域。借助NetPhos3.1在线软件预测磷酸化位点,利用SOMPA在线软件进行二级结构的预测。使用SWISS-MODEL在线软件对EgTP53的三级结构进行预测,采用IEDB在线软件进行B细胞抗原表位预测。使用MEGA6.0软件构建系统进化树并进行分析;采用实时荧光定量PCR技术检测去氢骆驼蓬碱作用后EgTP53蛋白的mRNA表达水平。结果分子对接结果显示,EgTP53与去氢骆驼蓬碱的对接能量为-4.44 kcal/mol,有良好的对接位点。生物信息学分析EgTP53氨基酸数量1108个,分子量121799.11,等电点9.29,属于亲水性蛋白,有一个信号肽,无跨膜区,有多个磷酸化位点,28个B细胞抗原表位。其中存在TUDOR蛋白结构域,存在2个BRCT保守蛋白结构域。EgTP53与亚洲带绦虫病、带状泡尾绦虫同属一个分支,与长膜带绦虫、矮小齿壳绦虫、微口膜壳绦虫同属绦虫纲,进化关系较近,与智人、家鼠、果蝇进化关系较远。实时荧光定量PCR结果显示去氢骆驼蓬碱干预组的EgTP53蛋白的mRNA表达较空白对照组明显上调。结论生物信息学分析表明EgTP53蛋白具有保守的功能结构域,对接点位稳定性良好,抗原表位多。

关键词：细粒棘球蚴 EgTP53蛋白生物信息学分子对接去氢骆驼蓬碱

How light affect the magnetotactic behavior and reproduction of ellipsoidal multicellular magnetoglobules?

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Journal of Oceanology and Limnology 2021年第6期39卷 2005-2014页

作者： Xinxin QIAN yicong zhao Claire-Lise SANTINI Hongmiao PAN Tian XIAO Haitao CHEN Tao SONG Jinhua LI Francois ALBERTO Sophie BRUSTLEIN Long-Fei WU State Key Laboratory of Microbial Resources Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China Aix Marseille University CNRSLCBCenturiIM2BIMMMarseille 13009France International Associated Laboratory of Evolution and Development of Magnetotactic Multicellular Organisms(LIA-MagMC) CNRS-CASMarseille 13402France CAS Key Laboratory of Marine Ecology and Environmental Sciences Institute of OceanologyChinese Academy of SciencesQingdao 266071China Institute of Electrical Engineering Chinese Academy of SciencesBeijing 100190China Key Laboratory of Earth and Planetary Physics Institute of Geology and GeophysicsChinese Academy of SciencesBeijing 100029China Aix Marseille University CenturiMarseille 13009France

Magnetotactic bacteria(MTB)synthesize intracellular magnetic organelles,magnetosomes,which consist of magnetic crystals that are enveloped in a *** are organized into a chain(s)and confer on cells a magnetic dipolar *** magnetic property allows MTB cells to align and swim along geomagnetic field lines,a movement referred to as *** MTB species change their swim direction in response to illumination by UV,violet and blue *** we analyzed the polarity of morphology,magnetism,and motion in Mediterranean multicellular magnetotactic prokaryotes,also called,magnetoglobules or *** magnetoglobules were assembled from 60-80 cells into an asymmetric ellipsoidal morphology with a relative narrow and large *** swam dominantly northward,parallel to the direction of the magnetic field,with the narrow-end as the leading *** response to a reversal in the direction of the magnetic field,they aligned quickly along the magnetic field lines and kept swimming ***,under constant illumination,385-nm UV light,magnetoglobules changed their swimming direction southward anti-parallel to the direction of the magnetic field,with the large-end as the leading *** change from a northward to southward direction occurred along with an increase of swimming speed.A minimum of 35-mW/cm^(2) irradiance of UV light was sufficient to trigger the swimming *** radiation also triggered the unidirectional division of *** these results revealed a coordination of the polarity of magnetoglobule morphology,magnetic moment,and swimming orientation,in response to magnetic and optical *** UV triggered the reversal of magnetotaxis and magnetoglobule division indicating the ecological significance of light for multicellular magnetotactic prokaryotes.

关键词： photo-response magnetic alignment coordinated swimming

Expansion and improvement of ChinaMu by MuT-seq and chromosome-level assembly of the Mu-starter genome

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Journal of Integrative Plant Biology 2024年第4期66卷 645-659页

作者： Lei Liang Yuancong Wang Yanbin Han yicong Chen Mengfei Li Yibo Wu Zeyang Ma Han zhao Rentao Song State Key Laboratory of Maize Bio-breeding Frontiers Science Center for Molecular Design BreedingJoint International Research Laboratory of Crop Molecular BreedingNational Maize Improvement CenterCollege of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100091China Institute of Crop Germplasm and Biotechnology Jiangsu Provincial Key Laboratory of AgrobiologyJiangsu Academy of Agricultural SciencesNanjing 210014China Sanya Institute of China Agricultural University Sanya 572025China Hainan Yazhou Bay Seed Laboratory Sanya 572025China

ChinaMu is the largest sequence-indexed Mutator(Mu)transposon insertional library in maize(Zea mays).In this study,we made significant improvements to the size and quality of the ChinaMu *** developed a new Mu-tag isolation method Mu-Tn5-seq(MuT-seq).Compared to the previous method used by ChinaMu,MuT-seq recovered 1/3 more germinal insertions,while requiring only about 1/14 of the sequencing volume and 1/5 of the experimental *** MuT-seq,we identified 113,879 germinal insertions from 3,168 Mu-active F1 *** also assembled a high-quality genome for the Mu-active line Mu-starter,which harbors the initial active MuDR element and was used as the pollen donor for the mutation *** the Mu-starter genome,we recovered 33,662(15.6%)additional germinal insertions in 3,244(7.4%)genes in the Mu-starter *** Mu-starter genome also improved the assignment of 117,689(54.5%)germinal *** newly upgraded ChinaMu dataset currently contains 215,889 high-quality germinal *** insertions cover 32,224 pan-genes in the Mu-starter and B73Ref5 genomes,including 23,006(80.4%)core genes shared by the two *** a test model,we investigated Mu insertions in the pentatricopeptide repeat(PPR)superfamily,discovering insertions for 92%(449/487)of PPR genes in ChinaMu,demonstrating the usefulness of ChinaMu as a functional genomics resource for maize.

关键词： ChinaMu Mutator MuT-seq PPR gene Zea mays

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应激性心肌病的研究进展

心血管病学进展 2024年第7期45卷 593-597页

作者：唐琛史宏志韩红亚刘晓丽赵亦骢王琛茜张佳宜首都医科大学北京100069 解放军总医院第一医学中心普通外科医学部北京100853 首都医科大学附属北京安贞医院老年心血管病中心北京100029

应激性心肌病是以可逆性室壁运动异常为特征的一种心血管综合征,临床表现与急性冠脉综合征相似,但冠状动脉造影显示冠状动脉解剖正常。应激性心肌病的发病机制尚未完全明确,临床诊断不易,标准治疗药物存在争议,因并发症和复发风险导致... 详细信息

应激性心肌病是以可逆性室壁运动异常为特征的一种心血管综合征,临床表现与急性冠脉综合征相似,但冠状动脉造影显示冠状动脉解剖正常。应激性心肌病的发病机制尚未完全明确,临床诊断不易,标准治疗药物存在争议,因并发症和复发风险导致预后不佳。现总结应激性心肌病的流行病学、发病机制、诊断和治疗方面的最新进展,以期为改善疾病的预后提供思路。

关键词：应激性心肌病儿茶酚胺嗜铬细胞瘤 β肾上腺素受体阻滞剂

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Comparison of enhancement techniques based on neural networks for attenuated voice signal captured by flexible vibration sensors on throats

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Nanotechnology and Precision Engineering 2022年第1期5卷 1-11页

作者： Shenghan Gao Changyan Zheng yicong zhao Ziyue Wu Jiao Li Xian Huang School of Precision Instruments and Opto-Electronics Engineering Tianjin University92 Weijin RoadTianjin 300072China High-Tech Institute Fan Gong-Ting South Street on the 12thQingzhou 262550China

Wearable flexible sensors attached on the neck have been developed to measure the vibration of vocal cords during ***,highfrequency attenuation caused by the frequency response of the flexible sensors and absorption of high-frequency sound by the skin are obstacles to the practical application of these sensors in speech capture based on bone *** this paper,speech enhancement techniques for enhancing the intelligibility of sensor signals are developed and *** kinds of speech enhancement algorithms based on a fully connected neural network(FCNN),a long short-term memory(LSTM),a bidirectional long short-term memory(BLSTM),and a convolutional-recurrent neural network(CRNN)are adopted to enhance the sensor signals,and their performance after deployment on four kinds of edge and cloud platforms is also *** results show that the BLSTM performs best in improving speech quality,but is poorest with regard to hardware *** improves short-time objective intelligibility(STOI)by 0.18 to nearly 0.80,which corresponds to a good intelligibility level,but it introduces latency as well as being a large *** CRNN,which improves STOI to about 0.75,ranks second among the four neural *** is also the only model that is able to achieves real-time processing with all four hardware platforms,demonstrating its great potential for deployment on mobile *** the best of our knowledge,this is one of the first trials to systematically and specifically develop processing techniques for bone-conduction speed signals captured by flexible *** results demonstrate the possibility of realizing a wearable lightweight speech collection system based on flexible vibration sensors and real-time speech enhancement to compensate for high-frequency attenuation.

关键词： Flexible electronics Vibration sensor Neural network Speech enhancement Deep learning

Tirofiban combined with Aspirin in the Treatment of Acute Penetrating Artery Territory Infarction (STRATEGY): protocol for a multicentre, randomised controlled trial

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Stroke & Vascular Neurology 2024年第1期9卷 75-81,I0047-I0052页

作者： Xiaoling Liao Shuo Feng yicong Wang Yuesong Pan Weiqi Chen Hui Qu Xingquan zhao Liping Liu Yongjun Wang Yilong Wang Department of Neurology Beijing Tiantan HospitalCapital Medical UniversityBeijingChina China National Clinical Research Center for Neurological Diseases Beijing TiantanHospitalCapital Medical UniversityBeijingChina Chinese Institute for Brain Research BeijingChina National Center for Neurological Diseases BeijingChina Advanced Innovation Center for Human Brain Protection Capital Medical UniversityBeijingChina Beijing Laboratory of Oral Health Capital Medical UniversityBeijingChina

Background Perforating artery territorial infarction(PAI)caused by branch atheromatous disease(BAD)is prone to recurrence and early progression without an effective and well-documented antiplatelet treatment ***,an adjunctive antiplatelet agent,has shown great potential to treat acute ischaemic ***,whether the combination of tirofiban and aspirin can improve the prognosis of PAI remains *** To explore an effective and safe antiplatelet regimen for reducing the risk of recurrence and early neurological deterioration(END)in PAI caused by BAD by comparing the tirofiban and aspirin combination with placebo and aspirin *** Tirofiban combined with Aspirin in the Treatment of Acute Penetrating Artery Territory Infarction(STRATEGY)trial is an ongoing multicentre,randomised,placebo-controlled trial in *** patients shall be randomly assigned to receive standard aspirin with tirofiban or placebo on the first day and standard aspirin from days 2 to *** primary endpoint is a new stroke or END within 90 *** primary safety endpoint is severe or moderate bleeding within 90 *** The STRATEGY trial will assess whether tirofiban combined with aspirin is effective and safe in preventing recurrence and END in patients with *** registration number NCT05310968.

关键词： Aspirin regimen centre