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Chinese herbal medicine Ginkgo biloba *** for ischemic stroke:An overview of systematic reviews and meta-analyses

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Journal of Integrative Medicine 2024年第2期22卷 163-179页

作者： Tian-tian Meng Ya-ping You Min Li Jian-bo Guo Xin-bin Song Jing-yi Ding Xiao-long Xie An-qi Li shang-jin Li Xiang-jun yin Peng Wang Zhe Wang Bao-liang Wang Qing-yong He Department of Cardiology Guang'anmen HospitalChina Academy of Chinese Medical SciencesBeijing 100032China Department of Rehabilitation Dongfang HospitalBeijing University of Chinese MedicineBeijing 100071China School of Chemistry and Chemical Engineering Shanghai Jiao Tong UniversityShanghai 200240China School of Chinese Medicine the University of Hong KongHong KongChina Graduate School Henan University of Chinese MedicineZhengzhou 450008Henan ProvinceChina Department of Acupuncture and Moxibustion Dongzhimen HospitalBeijing University of Chinese MedicineBeijing 100010China Department of Cardiology Guang’anmen HospitalChina Academy of Chinese Medical SciencesZhengzhou 450008Henan ProvinceChina School of Integrated Traditional Chinese and Western Medicine Southwest Medical UniversityHangzhou 310053Zhejiang ProvinceChina School of Basic Medical Sciences Zhejiang Chinese Medical UniversityHangzhu 310053Zhejang ProvinceChina Department of Neurology the First Affiliated Hospital of Henan University of Chinese MedicineZhengzhou 450003Henan ProvinceChina

Background:Ginkgo biloba ***(GBLPs)are a class of Chinese herbal medicine used in the adjuvant treatment of ischemic stroke(IS).Recently,several systematic reviews(SRs)and meta-analyses(MAs)of GBLPs for IS have been ***:This overview aims to assess the quality of related SRs and *** strategy:Pub Med,Embase,Cochrane Library,Web of Science,Chinese Biological Medicine,China National Knowledge Infrastructure,Wanfang,and Chinese Science and Technology Journals databases were searched from their inception to December 31,*** criteria:SRs and MAs of randomized controlled trials(RCTs)that explored the efficacy of GBLPs for patients with IS were *** extraction and analysis:Two independent reviewers extracted data and assessed the methodological quality,risk of bias(ROB),reporting quality,and credibility of evidence of the included SRs and MAs using A Measurement Tool to Assess Systematic Reviews 2(AMSTAR 2),Risk of Bias in Systematic Reviews(ROBIS),the Preferred Reporting Items for Systematic Reviews and Meta-analyses(PRISMA),and the Grading of Recommendations Assessment,Development and Evaluation(GRADE),***,descriptive analysis and data synthesis were ***:Twenty-nine SRs/MAs involving 119 outcomes were included in this *** overall methodological quality of all SRs/MAs was critically low based on AMSTAR 2,and 28 had a high ROB based on the *** to the PRISMA statement,the reporting items of the included SRs/MAs are relatively *** results based on GRADE showed that of the 119 outcomes,8 were rated as moderate quality,24 as low quality,and 87 as very low *** on the data synthesis,GBLPs used in conjunction with conventional treatment were superior to conventional treatment alone for decreasing neurological function ***:GBLPs can be considered a beneficial supplemental therapy for ***,because of the low quality of the existing eviden

关键词： statement synthesis alone

A structural and data-driven approach to engineering a plant cytochrome P450 enzyme

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Science China(Life Sciences) 2019年第7期62卷 873-882页

作者： Dawei Li Yongshuo Ma Yuan Zhou Junbo Gou Yang Zhong Lingling Zhao Lida Han Sergey Ovchinnikov Ling Ma Sanwen Huang Per Greisen yi shang Agricultural Genome Institute at Shenzhen Chinese Academy of Agricultural SciencesShenzhen 518124China School of Pharmacy Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Institute of Apicultural Research Chinese Academy of Agricultural SciencesBeijing 100093China Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China FAS Center for Systems Biology Harvard UniversityCambridge MA 02138USA The CAAS-YNNU-yiN MO RE Joint Academy of Potato Science Yunnan Normal UniversityYunnan 650500China Global Research Novo Nordisk A/SMalov DK-2760Denmark

Functional manipulation of biosynthetic enzymes such as cytochrome P450 s(or P450 s) has attracted great interest in metabolic engineering of plant natural *** and mogrosides are plant triterpenoids that share the same backbone but display contrasting *** structural and functional diversity of the two metabolites can be manipulated by engineering P450 ***,the functional redesign of P450 s through directed evolution(DE) or structure-guided protein engineering is time consuming and challenging,often because of a lack of high-throughput screening methods and crystal structures of P450 *** this study,we used an integrated approach combining computational protein design,evolutionary information,and experimental data-driven optimization to alter the substrate specificity of a multifunctional P450(CYP87 D20)from *** three rounds of iterative design and evaluation of 96 protein variants,CYP87 D20,which is involved in the cucurbitacin C biosynthetic pathway,was successfully transformed into a P450 mono-oxygenase that performs a single specific hydroxylation at C11 of *** integrated P450-engineering approach can be further applied to create a de novo pathway to produce mogrol,the precursor of the natural sweetener mogroside,or to alter the structural diversity of plant triterpenoids by functionally manipulating other P450 s.

关键词： plant P450 engineering protein design Rosetta amino acid co-evolution cucurbitacin mogroside

Enhanced chemoselectivity of a plant cytochrome P450 through protein engineering of surface and catalytic residues

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aBIOTECH 2021年第3期2卷 215-225页

作者： Xiaopeng Zhang Wei Luo yinying Yao Xuming Luo Chao Han Yang Zhong Bo Zhang Dawei Li Lida Han Sanwen Huang Per Greisen yi shang Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518116China Yunnan Key Laboratory of Potato Biology The CAAS-YNNU-YINMORE Joint Academy of Potato SciencesYunnan Normal UniversityKunming 650500China National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research Huazhong Agricultural UniversityWuhan 430070China Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of Ministry of Agriculture Sino-Dutch Joint Lab of Horticultural GenomicsInstitute of Vegetables and FlowersChinese Academy of Agricultural SciencesBeijing 100081China Novo Nordisk Research Center Seattle Inc SeattleWA 98109USA

Cytochrome P450s(P450s)are the most versatile catalysts utilized by plants to produce structurally and functionally diverse *** the high degree of gene redundancy and challenge to functionally characterize plant P450s,protein engineering is used as a complementarystrategy to study the mechanisms of P450-mediated reactions,or to alter their *** previously proposed an approach of engineering plant P450s based on combining high accuracy homology models generated by Rosetta combined with data-driven design using evoluti onary information of these *** this strategy,we repurposed a multi-functional P450(CYP87D20)into a monooxygenase after red esigning its active *** most plant P450s are membrane-anchored proteins that are adapted to the micro-environments of plant cells,expressing them in heterologous hosts usually results in problems of expression or ***,we applied compu-tational design to tackle these issues by simultaneous optimization of the protein surface and active *** screening 17 variants,effective su bstitutions of surface residues were observed to improve both expression and activity of *** addition,the identified substitutions were additive and by com-bining them a highly eficient C11 hydroxylase of cucurbitadienol was created to participate in the mogrol *** study shows the importance of considering the interplay between surface and active site residues for P450 *** integrated strategy also opens an avenue to create more tai loring enzymes with desired functions for the metabolic engineering of high-valued compounds like mogrol,the precursor of natural sweetener mogrosides.

关键词： Plant cytochrome P450 Protein engineering Rosetta Amino acid co-evolution Surface residue

Novel Farming Innovation for High Production of Black Pepper （Piper nigrum L.） Planting Materials

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Journal of Agricultural Science and Technology(B) 2017年第5期7卷 301-308页

作者： Kho Pei Ee Chen yi shang Malaysian Pepper Board Jalan Utama Pending Industrial Area P.O. Box 1653 Kuching Sarawak 93916 Malaysia

In accordance to National Commodity Policy (2011-2020), Malaysian government targets at expending the pepper cultivated area from the current 16,331 ha to 20,110 ha by year 2020. Based on a planting density of 2,000 vines/ha, there will be an urgent need of 7.588 million cuttings for the next five years in order to achieve this target. However, shortages of planting materials, low productivity and occurrence of pests and diseases remain as the major challenge of pepper industry nowadays. Therefore, a novel farming innovation, namely W-configuration cultivation method has been developed to ensure high production of planting materials for large-scale pepper cultivation and at the same time to maximize land use in pepper farm. A field evaluation was conducted to compare the cutting production among W-configuration, V-configuration and traditional cultivation method. W-configuration recorded the highest mean number of pepper cuttings produced with 11.97 cuttings per vine in every pruning cycle, or equivalent to 71% of increment as compared to 7.00 cuttings per vine produced from the traditional planting method. Meanwhile, V-configuration produced 8.91 of mean cuttings, which are significantly lower than those produced by W-configuration method and yet comparable to traditional method. Based on return on investment (ROI) analysis, implementation of W-configuration cultivation method was able to achieve 35% of return per cycle of planting within two years for the first cycle and upsurged to approximately 355% for the subsequence cycle. Furthermore, this novel innovation also out-yielded traditional planting method by 75.27% for the first and second year of green berry production. The ROI analysis proved the feasibility of this newly developed cultivation method to create potential new income for pepper smallholders in Malaysia, who traditionally cultivate pepper for its peppercorn production. This study also enlightened the thorough planting procedures of W-configuration

关键词：耕作方法生产率辣椒材料革新配置方法马来西亚生产栽培

星形胶质细胞、胶质纤维酸性蛋白及内皮抑素水平与重症创伤性脑损伤结局的关系探讨

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中国社区医师 2023年第8期39卷 13-15页

作者：商益惠州市中大惠亚医院重症监护室广东惠州516000

目的:探讨星形胶质细胞(AC)、胶质纤维酸性蛋白(GFAP)及内皮抑素(ES)水平与重症创伤性脑损伤(TBI)结局的关系。方法:选取105只健康雄性Wistar大鼠,采用随机数字表法分为健康组、TBI组、假手术组,各35只。TBI组采用改进的Feney’s自由落... 详细信息

目的:探讨星形胶质细胞(AC)、胶质纤维酸性蛋白(GFAP)及内皮抑素(ES)水平与重症创伤性脑损伤(TBI)结局的关系。方法:选取105只健康雄性Wistar大鼠,采用随机数字表法分为健康组、TBI组、假手术组,各35只。TBI组采用改进的Feney’s自由落体撞击法制作脑外伤模型,保证撞击后大鼠的神经功能损伤评分相同;假手术组仅切开头皮和颅骨开窗;健康组不做处理。各组分别在伤后6 h、12 h、24 h、3 d、7 d、14 d、21 d随机取出5只大鼠,应用改良神经功能损伤评分系统(mNSS)进行神经功能评分。常规苏木精-伊红染色法(HE)染色,采用免疫组织化学染色法作GFAP、ES染色,分别以AC、小胶质细胞的胞质内出现黄色或棕褐色颗粒为阳性细胞。结果:健康组及假手术组GFAP免疫组织化学染色AC胞质呈棕褐色,形如蜘蛛,周围有放射状突起;ES免疫组织化学染色无明显阳性细胞。TBI组HE染色,可见脑组织水肿,部分神经元及神经胶质细胞变性坏死,损伤3 d内,挫伤灶内细胞数目减少,随时间延长可见胶质细胞逐渐增生。TBI组GFAP阳性细胞平均面积随时间延长而逐渐增加,在7 d及14 d达到高峰,且TBI组阳性细胞平均面积大于健康组,差异有统计学意义(P0.05),伤后3~21 d ES含量逐渐增加,差异有统计学意义(P<0.05)。结论:可通过AC、GFAP及ES水平判断重症TBI结局。

关键词：重症创伤性脑损伤星形胶质细胞胶质纤维酸性蛋白内皮抑素

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口腔种植修复对牙列缺失患者的临床疗效研究

中国社区医师 2022年第20期38卷 30-32页

作者：易商长沙市口腔医院修复工艺科湖南长沙410000

目的:研究口腔种植修复对牙列缺失患者的临床疗效。方法:选取2018年9月-2020年6月长沙市口腔医院收治的64例牙列缺失患者作为研究对象,随机分为对照组和试验组,各32例。对照组给予义齿固定修复治疗,试验组给予口腔种植修复治疗,对比两... 详细信息

目的:研究口腔种植修复对牙列缺失患者的临床疗效。方法:选取2018年9月-2020年6月长沙市口腔医院收治的64例牙列缺失患者作为研究对象,随机分为对照组和试验组,各32例。对照组给予义齿固定修复治疗,试验组给予口腔种植修复治疗,对比两组临床疗效。结果:治疗前,两组患者咀嚼功能和美观度评分比较,差异无统计学意义(P>0.05);治疗后,试验组咀嚼功能和美观度评分均高于对照组,差异有统计学意义(P0.05);治疗后,试验组口腔健康影响程度各指标评分均低于对照组,差异有统计学意义(P<0.05)。试验组并发症发生率低于对照组,差异有统计学意义(P<0.05)。试验组生活质量评分高于对照组,差异有统计学意义(P<0.05)。结论:口腔种植修复可提高牙列缺失患者的治疗效果,改善患者生活质量,降低并发症发生率,值得临床深入研究。

关键词：口腔种植修复牙列缺失口腔健康

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高风险岩溶地区隧道施工风险安全预警方法

计算机仿真 2023年第12期40卷 519-523页

作者：尚毅广李立新吉林建筑科技学院土木工程学院吉林长春130114 沈阳建筑大学土木工程学院辽宁沈阳110168

由于岩溶地区地质条件复杂,不可预知性强,在隧道施工安全预警时预测精度难以保证,为此提出一种高风险岩溶地区隧道施工安全预警方法。通过对高风险岩溶地区隧道施工风险因素展开研究,构建施工安全预警体系,确定预警指标。采用层次分析... 详细信息

由于岩溶地区地质条件复杂,不可预知性强,在隧道施工安全预警时预测精度难以保证,为此提出一种高风险岩溶地区隧道施工安全预警方法。通过对高风险岩溶地区隧道施工风险因素展开研究,构建施工安全预警体系,确定预警指标。采用层次分析法和模糊综合评估方法对高风险岩溶地区隧道施工安全展开评估,获取施工风险等级,根据风险评估等级确定出对应的预警级别,实现高风险岩溶地区隧道施工安全预警。仿真结果表明,采用所提方法可以得到准确率更高的预警结果,且能够以更快的速度实现风险预警,为隧道施工安全预警提供全新的思路。

关键词：高风险岩溶地区隧道施工安全预警模糊综合评估层次分析法

Advancements in research on transcutaneous electrical acupoint stimulation for alleviating pelvic pain in women

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Gynecology and Obstetrics Clinical Medicine 2024年第1期4卷 65-72页

作者： yi Ling Wang Xin Lei Lu shang Wang Xiao Hong Chang Rong Zhang Hong Lan Zhu Department of Obstetrics and Gynecology Peking University People's HospitalBeijingChina Peking University Neuroscience Research Institute BeijingChina Department of Neurobiology Peking University School of Basic Medical SciencesBeijingChina

Background Pelvicpain isacommon complaint of discomfort in women,with an increasing incidence and seriously affecting patients'quality of *** etiology involves multiple systems and disciplines,and therefore treatment modalities are *** electrical acupoint stimulation(TEAS)therapy is a classic analgesic means,and electrical stimulation of local acupoints can *** to discuss the application of TEAS for the treatment of various types of pelvic pain,the selection of optimal parameters and the renewal of *** Result TEAS fortreatmentof pelvic pain in women has been reported mainly for endometriosis,pelvic inflammatory disease(PID),intrauterine adhesion(IUA),pelvic stasis syndrome,interstitial cystitis,primary dysmenorrhoea and other *** for parameter selection,the most used of frequency is dilatational wave,the most used stimulation intensity is 10-20 mA,and the acupoint selection should be based on the specific conditions of *** The analgesic mechanism of TEAS has not been fully elucidated,but the efficacy of the treatment is remarkable and thereare no significant adverse *** selection of the parameters for TEAS and the combinationof acupoints fordifferent pains are stillthe maindirections offutureresearch.

关键词： stimulation pain pelvic

Large aperture disk standing-wave unstable resonator with intra-cavity adaptive correction

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Chinese Optics Letters 2024年第5期22卷 57-61页

作者：于益易家玉徐浏尚建力吴晶童立新高清松唐淳张卫陈磊 Department of Optical Engineering Nanjing University of Science and TechnologyNanjing 210094China Graduate School of China Academy of Engineering Physics Beijing 100193China Institute of Applied Electronics China Academy of Engineering PhysicsMianyang 621900China

An unstable resonator with seven large aperture ceramic disks and intra-cavity adaptive correction is *** composite ceramic disks with absorption rings were adopted to suppress amplified spontaneous *** intra-cavity aberration non-conjugate correction based on round-trip wavefront and relaxation iteration was applied in the *** tilt and defocus were corrected in turn,an average output power of 4.5 kW was *** corresponding beam quality factorβwas *** tilt,defocus,and high order aberrations were corrected,the average output power was increased to 5.4 kW,and the beam quality factorβwas improved to 6.8.

关键词： Nd:YAG disk unstable resonator intra-cavity adaptive optics composite ceramic