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Single-molecule analysis in an electrochemical confined space

2008年

作者： Terry Tomscha Peggy Albers zhu Zhu Deguang yi.

来源：畅想之星图书光盘版（南... 评论

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Science China Chemistry 2017年第9期60卷 1187-1190页

作者： yi-Lun ying yi-Tao long Key Laboratory for Advanced Materials&School of Chemistry and Molecular Engineering East China University of Science and TechnologyShanghai 200237China

Electrochemical analysis of single molecules is a method with the strong ability of the enhanced efficiency and ***,we demonstrate that the electrochemical confined space could efficiently convert single molecule characteristics into measurable electrochemical signatures with high temporal *** human telomere repeat sequence T8 was used as a probe to determine the electrochemical confined effect in a *** results show that the nanopore with comparable confined space of the telomere repeat sequence exhibits the most distinguishable single-molecule signals which suggest the folded conformation of *** method will greatly extend the lifetime of a metastable conformation for a single biomolecule by strong analyte-nanopore interactions,which brings the new insight into the understanding of the biomolecule's function at single-molecule level.

关键词： electrochemical confined space, nanopores, single-molecule analysis, DNAs

Design and Control of a Quasi-direct Drive Actuated Knee Exoskeleton

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Journal of Bionic Engineering 2022年第3期19卷 678-687页

作者： yi long Yajun Peng Foshan Graduate School of Northeastern University Foshan528000China Faculty of Robot Science and Engineering Northeastern UniversityShenyang110000China Zhongshan Walkbest Intelligent Medical Device Co.Ltd Zhongshan528400China

This paper describes the design and control of a portable and lightweight knee exoskeleton for people with knee *** knee exoskeleton is designed based on our custom quasi-direct drive actuation composed of a DC motor unit and a transmission mechanism encompassing three *** online gait generation method based on gait step calculation and direct measurement using Inertial Measurement Unit(IMU)sensors is proposed to provide continuous assistance during *** on the generated gait trajectory online,Active Disturbance Rejection Control(ADRC)incorporating the feedforward compensation approach is proposed to help the human leg move in consideration of external *** developed knee exoskeleton has been employed successfully to assist impaired users with knee dysfunction to walk in a health recovery *** experimental results indicate that the online gait generation method and the proposed control method are suitable for the knee *** maximum value of the target knee angular position is approximately 50°and the mean of the control torque for the knee joint is located in the interval of[−2 Nm,4 Nm].The developed knee exoskeleton has the potential to regain normal gait to improve strength and endurance during walking in their activities of daily life.

关键词： Exoskeleton Knee dysfunction Quasi-direct drive Active disturbance rejection control Gait generation

Influence of multi-stage heat treatment on the microstructure and mechanical properties of TC21 titanium alloy

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International Journal of Minerals,Metallurgy and Materials 2021年第2期28卷 296-304页

作者： Wei long Song Zhang yi-long Liang Mei-gui Ou College of Materials and Metallurgy Guizhou UniversityGuiyang 550025China Key Laboratory for Materials Structure and Strength of Guizhou province Guizhou UniversityGuiyang 550025China National Local Joint Engineering Laboratory for High Performance Metal Structure Materials and Manufacturing Technology Guizhou UniversityGuiyang 550025China

Duplex-structured TC21 alloy samples were first solution-treated at a higher temperature in theα+βregion(940°C)with furnace cooling(FC),air cooling(AC),and water cooling(WC),followed by a second-stage solution treatment at a lower temperature in theα+βregion(900°C),and then finally aged at 590°*** effects of the morphology and quantity ofαphases on the structure and properties of the TC21 alloy after the different heat treatments were *** in-situ tensile deformation process and crack propagation behavior were observed using scanning electron microscopy(SEM).The quantity of equiaxedαphases as well as the thickness of lamellarαphases reduced,the tensile strength increased firstly and then decreased,the elongation decreased with the increasing cooling rate after the first-stage solution *** amount and size of lamellarαphases increased after the second-stage solution treatment because of sufficient diffusion of the alloying elements,thereby leading to increased tensile *** amount of dispersedαphases increased after the third-stage aging treatment owing to the increase in the nucleation rate,resulting in a noteworthy strengthening *** the third-stage aging treatment,the first-stage FC sample exhibited better mechanical properties because it contained more equiaxedαandβtrans phases than the first-stage AC and WC samples.

关键词： multi-stage heat treatment titanium alloy in-situ tension microstructure mechanical properties

Bainite isothermal transformation of Si–Mn–Mo alloy by phase structure factor calculation under different rare earth contents

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Rare Metals 2014年第4期33卷 427-432页

作者： Yan-Liang yi yi-long Liang Qi-Bing Tan The Key Laboratory for Mechanical Behavior and Microstructure of Materials School of Materials and MetallurgyGuizhou University

The effect of rare earth （RE） on bainite trans- formation mechanism is not yet unified in the academia. Therefore, further studying the effect of RE on bainite transformation mechanism will have important guiding significance for the development of bainite steels. In this paper, using differential dilatometer （DIL805A/D）, heat treatment was performed and C-curve was drawn. The effect of phase structure factor on bainite transformation under two different fractions of RE in Si-Mn-Mo bainite steels was investigated by the empirical electron theory （EET） of solids, molecules, dilatometry, and metallogra- phy. Experimental results show that RE makes Fe-C- RE segregation structural unit of Si-Mn-Mo bainite steels increase, i.e., the values of phase structure factor （na, FDC） increase, and delay bainite transformation due to the drag effect resulting from the segregation of RE ferrite island interphase and the retardation of RE to carbon diffusion in Si-Mn-Mo bainite steels. Beside this, C-curve is shifted to right and down, and microstructures of bainite ferrite and substructures are finer.

关键词： Phase structure factor C-curve Substructure Rare earths

Nanopore sensing system for high-throughput single molecular analysis

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Science China Chemistry 2018年第12期61卷 1483-1485页

作者： Zhen Gu yi-Lun ying yi-Tao long Key Laboratory for Advanced Materials & School of Chemistry and Molecular EngineeringEast China University of Science and Technology Shanghai 200237China

Nanopore technique enables a sensitive, label free, low cost and high throughput method for single-molecule analysis[1–4]. Usually, the nanopore is fabricated on an insulating membrane with electrolyte solution in two sides, which can act as the only pathway for the ionic flow. When a single molecule enters the pore under the applied biased potential, it will temporarily block the ionic current flow through the nanopore. By analyzing the amplitude, duration, shape and frequency spectra of ionic current modulations, nanopore detection could provide the characteristics of the analytes.

关键词： Nanopore sensing system high-throughput single molecular analysis

In situ deformation behavior of TC21 titanium alloy with different α morphologies(equiaxed/lamellar)

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Rare Metals 2021年第5期40卷 1173-1181页

作者： Wei long Mei-Gui Ou Xi-Qin Mao yi-long Liang College of Materials and Metallurgy Guizhou UniversityGuiyang 550025China Key Laboratory for Materials Structure and Strength of Guizhou Province Guizhou UniversityGuiyang 550025China National Local Joint Engineering Laboratory for High Performance Metal Structure Materials and Manufacturing Technology Guizhou UniversityGuiyang 550025China

To study the effect ofαphase morphology(equiaxedα(αE)/lamellarα(αL))on the in situ tensile behavior of TC21 alloy,the slip band,dislocation,crack initiation,and propagation were analyzed by scanning electron microscopy(SEM)with in situ tensile stage and transmission electron microscopy(TEM).The results show that the slip bands first concentrate in theαE phases and easily truncate at theα/βphase boundaries,whereas the slip bands move across theαlamellae in theαL *** are easily generated inαE orα/βphase boundaries with large plastic *** the quantity ofαL is more thanαE,the crack tip is more easily deflected atαphases orαclusters with different orientations,making the main crack propagation path more *** the volume fraction ratio ofαE toαL is~3:4,i.e.,the volume fraction ofαE is close to that ofαL,TC21 alloy exhibits better strength and slower crack propagation rate.

关键词： In situ tensile TC21 alloy Equiaxed Lamellar αPhase

The Existence of Two Closed Characteristics on Every Compact Star-shaped Hypersurface in R^4

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Acta Mathematica Sinica,English Series 2016年第1期32卷 40-53页

作者： Hui LIU yi Ming long School of Mathematical Sciences University of Science and Technology of China Chern Institute of Mathematics and LPMC Nankai University

Recently, Cristofaro-Gardiner and Hutchings proved that there exist at least two closed characteristics on every compact star-shaped hypersuface in R4. Then Ginzburg, Hein, Hryniewicz, and Macarini gave this result a second proof. In this paper, we give it a third proof by using index iteration theory, resonance identities of closed characteristics and a remarkable theorem of Ginzburg et at.

关键词： Compact star-shaped hypersurface closed characteristic Hamiltonian systems resonanceidentity multiplicity

Nanopore-based sensing interface for single molecule electrochemistry

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Science China Chemistry 2019年第12期62卷 1576-1587页

作者： Rui Gao Yao Lin yi-Lun ying yi-Tao long Department of Chemistry University of UtahSalt Lake CityUtah 84112USA School of Chemistry&Molecular Engineering East China University of Science and TechnologyShanghai 200237China State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023China

Nanopore techniques are experiencing a gallop since it walked out the notebook and show its charm on the science *** nanoscale pore offers a single-molecule resolution with a label-free and high-selective manner for the research of molecular structures,molecular dynamics,single-molecule reactions and for a variety of applications in biophysics and *** this review,we introduce the construction of three types of nanopore platforms along with the latest progress in DNA sensing,structure and dynamics analysis of peptides/proteins,and the detection of redox reactions with new sensing ***,we depict nanopore data processing methods which provide an insight of data mining under the background ofbig *** could fully expect the great impact of nanopore techniques on not only for DNA sequencing and sensing applications,but also in protein sequencing and clinical diagnostics.

关键词： nanopore single-molecule sensing nanoelectrochemistry

In memory of respected ***-Zhao Feng

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Journal of Palaeogeography 2023年第1期12卷 26-27页

作者： long-yi Shao China University of Mining and Technology(Beijing) BeijingChina

January 13,2023 Professor Feng left us in such a hurry,and his voice and smile will always remain in our *** I participated in ***'s carbonate petrology course in 1983,my communication with *** has not stopped,and I regard myself as Professor Feng's postgraduate student outside the program.I often participated in the seminars convened by ***,participated in the discussion of relevant issues,listened to his teachings,and benefited a lot.

关键词： carbonate outside stopped