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植物病原真菌m6A识别蛋白的挖掘与功能分析

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农业生物技术学报 2024年第6期32卷 1393-1403页

作者：何旭朱蒙芳董彩奕王炫杨梦雨巩校东魏淑珍谷守芹刘玉卫贾慧河北农业大学生命科学学院/河北省农业微生物生物信息利用技术创新中心/华北作物改良与调控国家重点实验室河北省湿地保护与绿色发展协同创新中心

N;6;-甲基腺嘌呤（N;6;-methyladenosine,m;6;A）修饰是真核生物中最常见的RNA修饰。包含YT521-B同源（YT521-B homology,YTH）结构域的蛋白可识别m;6;A修饰，从而介导RNA代谢。该类蛋白在植物病原真菌中的研究还未见报道。本研究利用... 详细信息

N;6;-甲基腺嘌呤（N;6;-methyladenosine,m;6;A）修饰是真核生物中最常见的RNA修饰。包含YT521-B同源（YT521-B homology,YTH）结构域的蛋白可识别m;6;A修饰，从而介导RNA代谢。该类蛋白在植物病原真菌中的研究还未见报道。本研究利用生物信息学方法，对127种植物病原真菌中的m;6;A识别蛋白进行了系统鉴定和分析，并对该类蛋白在玉米大斑病菌（Setosphaeria turcica）发育过程中的基因表达模式进行分析。结果发现，在供试的127个菌株中共鉴定了225个m;6;A识别蛋白，其中担子菌门中93%真菌含有2个m;6;A识别蛋白；理化性质分析发现，在鉴定的225个m;6;A识别蛋白中，有67.56%的蛋白呈碱性，且不同门及不同生活方式真菌的m;6;A识别蛋白酸碱性存在显著差异。系统发育分析发现，225个m;6;A识别蛋白可分为5组，其中2组与人类（Homo sapiens）具有较近的进化关系，相同门和相同生活方式真菌的m;6;A识别蛋白倾向于聚类在同一进化分支上；保守位点分析发现，YTH结构域序列在所有鉴定的m;6;A识别蛋白中存在多个保守型位点。在玉米大斑病菌中鉴定了1个编码该类蛋白的基因St YTH1，表达量分析发现，其在病菌附着胞发育时期的表达量最高，可能对病菌附着胞发育具有重要的调控作用。本研究为深入揭示m;6;A识别蛋白的功能提供了基础资料。

关键词：植物病原真菌 N6-甲基腺嘌呤(m6A)识别蛋白生物信息学分析表达模式

Different lymph node staging systems for predicting the prognosis of colorectal neuroendocrine neoplasms

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World Journal of Gastrointestinal Oncology 2024年第5期16卷 1745-1755页

作者： Yuan-yi Zhang Yue-Wei cai Xia Zhang Department of Pathology Zhaoqing Medical CollegeZhaoqing 526020Guangdong ProvinceChina Department of Emergency Zhaoqing Second People’s HospitalZhaoqing 526020Guangdong ProvinceChina Department of Pathology and Physiology Zhaoqing Medical CollegeZhaoqing 526020Guangdong ProvinceChina

BACKGROUND Colorectal neuroendocrine neoplasms(NENs)are a rare malignancy that primarily arises from the diffuse distribution of neuroendocrine cells in the colon and *** studies have pointed out that the status of lymph node may be used to predict the *** To investigate the predictive values of lymph node ratio(LNR),positive lymph node(PLN),and log odds of PLNs(LODDS)staging systems on the prognosis of colorectal NENs treated surgically,and compare their predictive *** This cohort study included 895 patients with colorectal NENs treated surgically from the Surveillance,Epidemiology,and End Results *** endpoint was mortality of patients with colorectal NENs treated surgically.X-tile software was utilized to identify most suitable thresholds for categorizing the LNR,PLN,and *** were selected in a random manner to form training and testing *** prognosis of surgically treating colorectal NENs was examined using multivariate cox analysis to assess the associations of LNR,PLN,and LODDS with the prognosis of colorectal NENs.C-index was used for assessing the predictive *** conducted a subgroup analysis to explore the different lymph node staging systems’predictive *** After adjusting all confounding factors,PLN,LNR and LODDS staging systems were linked with mortality in patients with colorectal NENs treated surgically(P<0.05).We found that LODDS staging had a higher prognostic value for patients with colorectal NENs treated surgically than PLN and LNR staging *** results were obtained in the different G staging subgroup ***,the area under the receiver operating characteristic curve values for LODDS staging system remained consistently higher than those of PLN or LNR,even at the 1-,2-,3-,4-,5-and 6-year follow-up *** LNR,PLN,and LODDS were found to significantly predict the prognosis of patients with colorectal NENs treated surgically.

关键词： Positive lymph node Lymph node ratio Log odds of positive lymph nodes Prognosis Colorectal neuroendocrine neoplasms

跨专业课程群建设的探索与实践——以设计类课程为例

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绿色包装 2024年第1期 50-53页

作者：陈赛艳陈坤易俊材陈洪吴贺君四川农业大学四川雅安625014

根据四新建设“深度交叉再出新”的宗旨,按照协同育人的教学要求,在原有“跨学院授课、多教师组课”的基础上,对标OBE教育理念,构建“学校-学院-教师”三级联动机制、“学科-专业-课程”三维建设体系,采用“线上-线下”混合式教学平台,... 详细信息

根据四新建设“深度交叉再出新”的宗旨,按照协同育人的教学要求,在原有“跨学院授课、多教师组课”的基础上,对标OBE教育理念,构建“学校-学院-教师”三级联动机制、“学科-专业-课程”三维建设体系,采用“线上-线下”混合式教学平台,通过分析设计类课程的内在联系,提出以课程的内在联系为基础,开展跨专业课程群建设。所构建的课程群逻辑结构合理、课程融合度高、课程交叉性强、能力导向明确。

关键词：课程群跨专业教学改革

负载雷公藤红素的中空介孔聚多巴胺纳米复合物的制备及体外抗肿瘤活性研究

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中南药学 2024年第5期22卷 1119-1124页

作者：潘慕倩蔡义思袁立霞余林中南方医科大学中医药学院广州510515

目的制备负载雷公藤红素的中空介孔聚多巴胺纳米复合物(H-PDA/Cel@HA),并考察其体外抗肿瘤活性研究。方法采用湿化学刻蚀法制备H-PDA/Cel@HA,通过粒径、Zeta电位、透射电镜、紫外吸收光谱等方法对其进行表征,并从细胞毒性、细胞活性... 详细信息

目的制备负载雷公藤红素的中空介孔聚多巴胺纳米复合物(H-PDA/Cel@HA),并考察其体外抗肿瘤活性研究。方法采用湿化学刻蚀法制备H-PDA/Cel@HA,通过粒径、Zeta电位、透射电镜、紫外吸收光谱等方法对其进行表征,并从细胞毒性、细胞活性氧水平测定、活/死细胞染色实验、细胞凋亡等方面评价H-PDA/Cel@HA对乳腺癌4T1细胞的抗肿瘤活性。结果所制备的H-PDA/Cel@HA呈现出中空立方体的形貌,平均粒径为(183.4±12.3)nm,Zeta电位为(-30.4±1.1)mV;载药量为(13.25±1.16)%,包封率为(66.27±5.78)%;H-PDA/Cel@HA显著抑制乳腺癌4T1细胞的生长,相较于游离的Cel,其对正常细胞(L929)具有更好的生物相容性。此外,H-PDA/Cel@HA还通过增加4T1细胞内的活性氧水平,诱导细胞凋亡。结论所制备的H-PDA/Cel@HA具有良好的生物相容性和肿瘤靶向递送能力,可有效抑制肿瘤细胞的生长。

关键词：雷公藤红素中空聚多巴胺活性氧细胞凋亡

Radiomics in the diagnosis and treatment of hepatocellular carcinoma

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Hepatobiliary & Pancreatic Diseases International 2023年第4期22卷 346-351页

作者： Chun Jiang yi-Qi cai Jia-Jia Yang Can-Yu Ma Jia-Xi Chen Lan Huang Ze Xiang Jian Wu Department of Clinical Laboratory The Affiliated Suzhou Hospital of Nanjing Medical UniversitySuzhou Municipal HospitalGusu SchoolNanjing Medical UniversitySuzhou 215008China Zhejiang University School of Medicine Hangzhou 310030China Department of Infection Management The Affiliated Suzhou Hospital of Nanjing Medical UniversitySuzhou Municipal HospitalGusu SchoolNanjing Medical UniversitySuzhou 215008China

Hepatocellular carcinoma(HCC)is a common malignant *** present,early diagnosis of HCC is dif-ficult and therapeutic methods are *** can achieve accurate quantitative evaluation of the lesions without invasion,and has important value in the diagnosis and treatment of *** fea-tures can predict the development of cancer in patients,serve as the basis for risk stratification of HCC patients,and help clinicians distinguish similar diseases,thus improving the diagnostic ***-more,the prediction of the treatment outcomes helps determine the treatment *** is also helpful in predicting the HCC recurrence,disease-free survival and overall *** review summa-rized the role of radiomics in the diagnosis,treatment and prognosis of HCC.

关键词： Hepatocellular carcinoma Radiomics Diagnosis Prognosis Treatment

慢性病资源利用在冠心病PCI术后患者自我超越与自我管理行为间的中介效应

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护理学报 2024年第6期31卷 13-17页

作者：李爱群李利花王芳蒋源元蔡益民湖南师范大学医学院护理系湖南长沙410013 湖南省人民医院(湖南师范大学附属第一医院)心血管内科二病区湖南长沙410005 湖南省人民医院(湖南师范大学附属第一医院)护理部湖南长沙410005

目的分析慢性病资源利用在冠心病PCI术后患者自我超越与自我管理行为间的中介作用。方法采用一般资料调查表、自我超越量表、慢性病资源调查问卷、冠心病自我管理行为量表对720例冠心病PCI术后患者进行问卷调查。结果冠心病PCI术后... 详细信息

目的分析慢性病资源利用在冠心病PCI术后患者自我超越与自我管理行为间的中介作用。方法采用一般资料调查表、自我超越量表、慢性病资源调查问卷、冠心病自我管理行为量表对720例冠心病PCI术后患者进行问卷调查。结果冠心病PCI术后患者自我超越、慢性病资源利用、自我管理行为总分分别为(43.70±5.91)分、(55.12±8.37)分和(72.57±8.17)分;自我超越与慢性病资源利用呈正相关(r=0.562,P<0.01),两者与自我管理行为均呈正相关(r分别为0.516、0.717,P均<0.01);慢性病资源利用在自我超越与自我管理行为间起部分中介作用,中介效应占总效应的68.02%。结论冠心病PCI术后患者自我超越可以直接影响自我管理行为,也可以通过慢性病资源利用发挥作用。

关键词：冠心病患者冠状动脉介入治疗自我超越慢性病资源利用自我管理行为中介效应

Microscopic oil occurrence in high-maturity lacustrine shales:Qingshankou Formation,Gulong Sag,Songliao Basin

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Petroleum Science 2023年第5期20卷 2726-2746页

作者： Jing-Ya Zhang Ru-Kai Zhu Song-Tao Wu Xiao-Hua Jiang Chang Liu yi cai Su-Rong Zhang Tian-Shu Zhang Research Institute of Petroleum Exploration and Development PetroChinaBeijing100083China

Occurrence and mobility of shale oil are prerequisites for evaluating shale oil reserves and prioritizing exploration targets,particularly for heterogeneous lacustrine *** Qingshankou Formation in the Gulong Sag,Songliao Basin is a classic lacustrine pure shale reservoir that contains abundant shale oil *** predicted geological reserves of the shale are 1.268×10^(9) *** this study,field emission scanning electron microscope(FE-SEM),the modular automated processing system(MAPS),pyrolysisgas chromatography(Py-GC),low-pressure nitrogen gas adsorption(LPNA),Soxhlet extraction,pyrolysis,and 2-D nuclear magnetic resonance(NMR)were integrated to describe the shale oil components,microscopic occurrence,mobility,and the effective pore size ***,the related controlling factors are *** shale oil in the Qingshankou Fm exists dominantly in the matrix pores of the clay minerals,with small amounts distributed in the intergranular pores of terrigenous clastic grains,intercrystalline pores of pyrite,intragranular pores of ostracod shells,and *** oil is distributed in the pore spaces of variable sizes in different *** clay mineral-laminated shales are characterized by the broadest range of pore size and largest volume of pore spaces with shale oil distribution,while the ostracod-laminated shales have limited pore spaces retaining ***,the proposed integrated analysis evaluates the shale oil molecules existing in two states:movable,and adsorbed oil,*** result illustrates that movable oil takes up 30.6%e79.4%of the total residual ***,mineral composition,and pore structures of the shale joint together to control the states and mobility of the shale *** values are positively correlated with the quantities of shale oil regardless of the state of *** mineral components significantly impact the state of shale *** differences in the states of oil were observed following

关键词： Shale oil content Occurrence states Micro-oil distribution Effective pore spaces Controlling factors Gulong sag

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一种多晶硅球阀技术改进方案

阀门 2024年第1期 97-100页

作者：杨积海吴孔燕周星彪丁远茂蔡毅特技阀门集团有限公司浙江温州325103 保一集团有限公司浙江温州325103

多晶硅专用球阀是多晶硅生产系统中的核心装备,具有截断到导通作用。由于其所处工况极其恶劣,使用寿命一般在三个月到半年,阀门密封失效极快。收集大量素材和用户反馈,通过不断优化密封面成型工艺、开发气固相分离技术、改善垫片密封形... 详细信息

多晶硅专用球阀是多晶硅生产系统中的核心装备,具有截断到导通作用。由于其所处工况极其恶劣,使用寿命一般在三个月到半年,阀门密封失效极快。收集大量素材和用户反馈,通过不断优化密封面成型工艺、开发气固相分离技术、改善垫片密封形式、简化阀杆受力结构、转移漏点等五大措施,系统性地解决了阀门内漏和外漏的问题。开发的多晶硅硬密封球阀完全实现了国产化,产品价格约为进口产品的76%,供货周期从进口的半年下降至2~3个月,产品使用寿命从6个月提高到两年以上,实现了以国代进,性能领先进口同类产品。

关键词：多晶硅硬密封球阀激光熔覆粉末过滤壳体密封阀杆密封

Probing the origin of our universe through primordial gravitational waves by Ali CMB project

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Science China(Physics,Mechanics & Astronomy) 2016年第7期59卷 90-92页

作者： yi-Fu cai XinMin Zhang CAS Key Laboratory for Researches in Galaxies and Cosmology Department of Astronomy University of Science and Technology of ChinaChinese Academy of Sciences Theoretical Physics Division Institute of High Energy Physics Chinese Academy of Sciences

Gravitational waves(GW),which were predicted by Einstein in 1916 based on the classical theory of General Relativity(GR),were recently detected by LIGO[1].This breakthrough is expected to initiate a novel probe of cosmology,the nature of gravity as well as fundamental *** general,signals of GWs can be classified into two categories。

关键词：宇宙起源引力波招商项目阿里天文物理经典理论爱因斯坦宇宙探测