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Molecular Cloning and Preliminary Analysis of a Fragile Site Associated Gene

Biomedical and Environmental Sciences 2006年第5期19卷 392-398页

作者： yi-wen cao CHUAN-LU JIANG TAO JIANG Department of Echography People＇s Armed Police General Hospital of Heilongjiang Province Harbin 150076 Heilongjiang China Department of Neurosurgery the Second Affiliated Hospital Harbin Medical University Harbin 150001 Heilongjiang China Department of Neurosurgery Neurosurgery Institute and Tiantan Hospital PUMC and CAMS Beijing 100050 China

Objective To analyze the molecular coining of a fragile site-associated gene. Methods Genomic Chinese hamster ovary （CHO） DNA library was constructed using high molecular weight CHO DNA partially digested with MboI restriction enzyme from cultured CHO cells. Screening of genomic DNA library followed the established procedures. Genomic CHO in the positive clones was sequenced. Appropriate primers were designed for the reverse transcriptase-polymerase chain reactions （RT-PCR）. The RT-PCR products were cloned into a pCRII TOPO vector and confirmed by DNA sequencing. Antibodies were prepared using synthetic peptides as antigens by immunizing the rabbits. Immunohistochemical analyses were performed to evaluate the expression of the novel gene in different tissues. Results To investigate the molecular mechanism underlying the initial events of mdrla amplification, we cloned lq31 fragile site DNA. Strikingly, we found that this fragile site contained a novel gene which was designated as a fragile site-associated （FSA） gene. FSA encoded an unusually large mRNA of - 16 kb. Full-length human FSA eDNA was cloned. FSA mRNA was expressed in many cultured cells and tissue types. Immunohistochemical analyses also revealed an expression pattern of the encoded proteins in postmitotic, well-differentiated epithelial compartments of many organs, including colon, mammary glands, ovary, prostate, and bladder. Conclusion FSA plays an important role in regulating mammalian epithelial cell growth and differentiation.

关键词： Fragile site DNA amplification Epithelial differentiation

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Rare earth element geochemistry in soils along arid and semiarid grasslands in northern China

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Ecological Processes 2022年第1期11卷 386-399页

作者： yi‑wen cao Xiao‑Ming Liu Chao Wang Edith Bai Nanping Wu CAS Key Laboratory of Forest Ecology and Management Institute of Applied EcologyChinese Academy of SciencesShenyang 110016China University of Chinese Academy of Sciences Beijing 100049China Department of Earth Marine and Environmental SciencesUniversity of North Carolina‑Chapel HillChapel HillNC 27515USA Key Laboratory of Geographical Processes and Ecological Security of Changbai Mountains School of Geographical SciencesNortheast Normal UniversityChangchun 130024China Institute of Deep‑Sea Science and Engineering Chinese Academy of SciencesSanya 572000China

Background:Rare earth elements(REE)are a group of trace elements that behave geochemically *** fractionation patterns normalized to reference materials provide a powerful tool for documenting *** processes are particularly difficult to illustrate with respect to contemporary and past climate *** this study,we characterize the rare earth element(REE)contents in bulk soils and respective geochemical fractions(e.g.,exchangeable,carbonate‑bound,reducible,and oxidizable fractions)and to decipher the relationships between REE geochemistry components and climatic factors across a large‑scale northern China transect(NCT).Results:Across the NCT,bulk REE concentrations ranged from 55.2 to 241.1μg g^(−1)with a main portion in the residual fraction(49–79%),followed by oxidizable fraction(2–40%),reducible fraction(3–22%),carbonate‑bound fraction(0.1–16%),and negligible exchangeable *** REE contents of geochemical components(carbonate‑bound,reducible,and oxidizable)in topsoils correlated to climate factors(mean annual precipitation,mean annual temperature,potential evaporation,and aridity index(AI)).The normalized abundances to the upper continental crust(UCC)composition show that the middle REE was generally enriched than the light REE and heavy REE in topsoils along the *** overall UCC‑normalized bulk REE patterns in topsoils and subsoils were similar,characterized by weak negative Ce anomalies and positive Eu ***:Our data in topsoils and depth profiles collectively suggest that cycling of REE was primarily regulated by abiotic processes in area with AI*** similar UCC normalized patterns in topsoils suggest that the REE was originated from a common source with limited influences from other sources(e.g.,atmospheric dusts and anthropogenic contribu‑tions).Our results to some extent provide evidence for

关键词： Rare earth elements Soil Grassland Northern China Aridity index

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The effect of increased intra-abdominal pressure on orbital subarachnoid space width and intraocular pressure

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Neural Regeneration Research 2018年第2期13卷 353-359页

作者： Su-meng Liu Ning-li Wang Zhen-tao Zuo Wei-wei Chen Di-ya Yang Zhen Li yi-wen cao Beijing Tongren Eye Center Beijing Tongren Hospital Capital Medical University Beijing Ophthalmology and Visual Sciences Key Laboratory Beijing Institute of Ophthalmology Beijing Tongren Hospital Capital Medical University State Key Laboratory of Brain and Cognitive Science Institute of Biophysics Chinese Academy of Sciences Department of Neurology Xuanwu Hospital Capital Medical University

In accordance with the trans-lamina cribrosa pressure difference theory, decreasing the trans-lamina cribrosa pressure difference can re- lieve glaucomatous optic neuropathy. Increased intracranial pressure can also reduce optic nerve damage in glaucoma patients, and a safe, effective and noninvasive way to achieve this is by increasing the intra-abdominal pressure. The purpose of this study was to observe the changes in orbital subarachnoid space width and intraocular pressure at elevated intra-abdominal pressure. An inflatable abdominal belt was tied to each of 15 healthy volunteers, aged 22-30 years （12 females and 3 males）, at the navel level, without applying pressure to the abdomen, before they laid in the magnetic resonance imaging machine. The baseline orbital subarachnoid space width around the optic nerve was measured by magnetic resonance imaging at 1, 3, 9, and 15 mm behind the globe. The abdominal belt was inflated to increase the pressure to 40 mmHg （1 mmHg = 0.133 kPa）, then the orbital subarachnoid space width was measured every 10 minutes for 2 hours. After removal of the pressure, the measurement was repeated 10 and 20 minutes later. In a separate trial, the intraocular pressure was measured for all the subjects at the same time points, before, during and after elevated intra-abdominal pressure. Results showed that the baseline mean orbital subarachnoid space width was 0.88 ＋ 0.1 mm （range： 0.77-1.05 mm）, 0.77 ＋ 0.11 mm （range： 0.60-0.94 mm）, 0.70 ＋ 0.08 mm （range： 0.62-0.80 ram）, and 0.68 _＋ 0.08 mm （range： 0.57-0.77 mm） at 1, 3, 9, and 15 mm behind the globe, respectively. During the elevated intra-abdominal pressure, the orbital subarachnoid space width increased from the baseline and dilation of the optic nerve sheath was significant at 1, 3 and 9 mm behind the globe. After decompression of the abdominal pressure, the orbital subarachnoid space width normalized and returned to the baseline value. There was no significant difference in the intra

关键词： nerve regeneration intraocular pressure intra-abdominal pressure intracranial pressure trans-lamina cribrosa pressure difference orbital subarachnoid space width magnetic resonance imaging optic nerve sheath glaucoma cerebrospinal fluid pressure subarachnoid space neural regeneration

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Aperiodically semi-intermittent-based fixed-time stabilization and synchronization of delayed discontinuous inertial neural networks

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Science China(Information Sciences) 2025年第1期68卷 238-254页

作者： Guodong ZHANG Jinde cao School of Mathematics and Statistics South-Central Minzu University School of Mathematics Southeast University

This article concentrates on the fixed-time stabilization(FS) and fixed-time synchronization(FTS) of discontinuous inertial neural networks(DINNs) with distributed delays. Using mathematical induction to a new differential equality, a novel fixed-time stability lemma is constructed. Then, by designing an aperiodically semi-intermittent switching control and combining it with the theory of nonsmooth analysis, some novel criteria on the FS and FTS of DINNs are obtained. Unlike the methods used in most existing studies, the FS and FTS results are structured using the newly proposed fixed-time stability lemma and the nonreduced-order approach, resulting in broader practical applications and strengthening the scientific quality of the derived results. Lastly, numerical simulations and applications proffer the effectiveness of the established FS and FTS criteria.

关键词： inertial neural networks fixed-time synchronization semi-intermittent switching control nonreduced-order approach fixed-time stabilization

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Deciphering fungal metabolon coupling tandem inverse-electrondemand Diels-Alder reaction and semipinacol rearrangement for the biosynthesis of spiro polycyclic alkaloids

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Science China Chemistry 2025年第1期68卷 288-296页

作者： Shuai Liu wen-Qiang Xu ying-Tong Di Man-Cheng Tang Ding-Kang Chen Ming-Ming cao Yao-wen Chang Hong-Yu Tang Chun-Mao Yuan Jun-Bo Yang Zhi-Li Zuo Han Guo Zi-Fei Xu ying Zeng Yun-Dong Wu Xiao-Jiang Hao Key Laboratory of Phytochemistry and Natural Medicines (Chinese Academy of Sciences) Kunming Institute of Botany Chinese Academy of Sciences University of Chinese Academy of Sciences Lab of Computational Chemistry and Drug Design State Key Laboratory of Chemical Oncogenomics Peking University Shenzhen Graduate School Shenzhen Bay Laboratory State Key Laboratory of Microbial Metabolism School of Life Sciences & Biotechnology Shanghai Jiao Tong University State Key Laboratory of Functions and Applications of Medicinal Plants Guizhou Medical University Molecular Biology Experimental Center Germplasm Bank of Wild Species in Southwest China Kunming Institute of Botany Chinese Academy of Sciences Yunnan Key Laboratory for Wild Plant Resources Kunming Institute of Botany Chinese Academy of Sciences Research Unit of Chemical Biology of Natural Anti-Virus Products Chinese Academy of Medical Sciences

In the intricate process of natural product biosynthesis, a metabolon can enhance metabolic flux by associating sequential enzymes. A fungal metabolon, comprising of flavin-dependent monooxygenase Spe F and P450 monooxygenase Spe G, is identified in the biosynthesis of spiro polycyclic alkaloids(+)-notoamide B and its diastereomer(+)-versicolamide B. Using notoamide E as a substance, Spe F/Spe G metabolon can control the stereoselectivity of its 2,3-epoxidation, followed by hydrogen atom ion at C-17 to generate reactive epoxide tau-m A with dienyl iminium unit. Subsequently,(+)-notoamide B and(+)-versicolamide B are produced via tandem nonenzymatic inverse-electron-demand Diels-Alder reaction and semipinacol rearrangement. This provides the first example of metabolon in the biosynthesis of spiro-prenylated indole alkaloids.

关键词： metabolon Diels-Alder reaction semipinacol rearrangement biosynthesis notoamides spiro polycyclic alkaloids

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Soliton Solutions of a Coupled KdV System via Backlund Transformation

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应用数学 2025年第1期38卷 211-216页

作者： cao Xifang WU yiheng LU yi XU wenjing XIA Yutong School of Mathematical Sciences Yangzhou UniversityYangzhou 225002China

In this paper we use Böcklund transformation to construct soliton solutions for a coupled KdV *** system was first proposed by Wang in *** we generalize the well-known Bäcklund transformation for the KdV equation to such coupled KdV *** from a trivial seed solution,we construct soliton *** also give a nonlinear superposition formula,which allows us to generate multi-soliton solutions.

关键词： KdV equation Coupled KdV system B¨acklund transformation Soliton

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Experimental Observing Damage Evolution in Cement Pastes Exposed to External Sulfate Attack by in situ X-ray Computed Tomography

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Journal of Wuhan University of Technology(Materials Science) 2025年第1期40卷 164-170页

作者： WU Min cao Kailei XIAO Weirong YU Zetai cao Jierong DING Qingjun LI Jinhui China Railway Construction Corporation Harbour and Channel Engineering Bureau Group Co. LTDZhuhai 519000China Con-struction Headquarters of Zhoushan Liuheng Bridge Phase II Project Zhoushan 316000China China Railway Construction Bridge En-gineering Bureau Group Co. LTDTianjin 300000China State Key Laboratory of Silicate Material for Architecture(Wuhan University of Technology) Wuhan 430070China College of Materials Science and Engineering Wuhan Textile UniversityWuhan 430200China

The paper presents experimental investigation results of crack pattern change in cement pastes caused by external sulfate attack(ESA).To visualize the formation and development of cracks in cement pastes under ESA,an X-ray computed tomography(X-ray CT)was used,i e,the tomography system of Zeiss Xradia 510 *** results indicate that X-CT can monitor the development process and distribution characteristics of the internal cracks of cement pastes under ESA with attack *** addition,the C3A content in the cement significantly affects the damage mode of cement paste specimens during sulfate *** damage of ordinary Portland cement(OPC)pastes subjected to sulfate attack with high C3A content are severe,while the damage of sulfate resistant Portland cement(SRPC)pastes is much smaller than that of OPC ***,a quadratic function describes the correlation between the crack volume fraction and development depth for two cement pastes immermed in sulfate solution.

关键词： concrete external sulfate attack damage evolution situ X-ray computed tomography

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Si-C Foam Porous-Medium Combustion Power-Generation System for Low-Calorific-Value Biomass Syngas

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Journal of Thermal Science 2025年第1期34卷 206-222页

作者： YANG Jianwen CHEN Wei cao Bingwei LIU Xinhui LI Hang College of Mechanical and Aerospace Engineering Jilin University School of Mechanical and Vehicle Engineering Changchun University

Syngas produced from the gasification of organic feedstocks from biomass is one of the clean and sustainable sources of *** advantages of simple access and renewability of biomass energy can meet the energy needs of temporary power *** study presents a biomass power-generation system for vehicular *** biomass and a free-piston Stirling engine generator（FPSEG） as the primary material and prime mover,respectively,biomass energy is converted into electricity by combusting the syngas to heat the *** and key parameter design for biomass gasification and thermoelectric conversion systems within a power generation system were performed.A porous medium area was constructed using Si-C foam ceramics to obtain an energy-conversion experimental *** effects of bed height,porosity,porous-region diameter,and air-intake conditions on the power-generation performance were investigated,and optimisations were performed for the thermoelectric conversion *** rate of increase during FPSEG power generation first increased and then decreased with increasing bed height,peaking at a bed height of 40 *** increasing porous-region diameter accelerated FPSEG power generation,whereas porosity changes in the porous media did not significantly affect the rate of change during FPSEG power *** increasing air intake,the rate of increase during power generation first increased and then *** maximum change rate and the highest thermoelectric conversion efficiency of the power-generation system occurred at 9.5 m3/h and 6.5 m3/h（～45.1%）air intakes,*** the thermal inertia and combustion structure of the thermoelectric conversion system significantly increased the power-generation rate of the system,with 1.8 W/s being observed at a 9.7 m3/h air intake.

关键词： biomass combined heat and power (CHP) gasification porous media combustion power generation

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Melting Evaluation of Phase Change Materials Impregnated into Cascaded Metal Foams with Regionalized Enhancement

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Journal of Thermal Science 2025年第1期34卷 223-241页

作者： WEI Xinyi ZHANG Nan ZHANG Zhaoli cao Xiaoling YUAN Yanping School of Mechanical Engineering Southwest Jiaotong University

Metal foam promotes the heat transfer of phase change materials(PCMs) in the penalty of reducing the energy storage density of the composite *** this work,the effects of constant porosity(0.96,0.94,0.92,or 0.90) and pore density(PPI) of metal foam on heat transfer of composite PCMs are *** rate could be enhanced by employing with low porosity copper ***,aiming to the right bottom phase changing "dead region",a regionalized enhancement strategy of cascaded metal foams is *** dynamic melting performances of all the composite PCMs are comprehensively *** results reveal that the cascaded configuration is beneficial for *** show that the horizontal strategy enhances melting performance:a maximum of 17.98% reduction in total melting time could be reached when the rear part porosity is 90%.The energy storage density rate could be raised by 5.48%.Besides,the vertical strategy performs with a better average temperature uniformity of 0.441 and brings a lower temperature in the heated *** sum up,the regionalized enhancement of copper foam provides better performance in the phase change *** shows significant potential for solar heat storage and thermal protection.

关键词： copper metal foam heat transfer enhancement phase change materials regionalized enhancement

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lncRNA SNHG4 enhanced gastric cancer progression by modulating miR-409-3p/CREB1 axis

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Oncology Research 2025年第1期33卷 185-198页

作者： ZHOUYANG CHENG YUCHEN HUA YANG cao JUN QIN Department of General Surgery Affiliated Hospital of Nantong UniversityNantong226001China Department of Surgery Affiliated Hospital of Nantong UniversityMedical School of Nantong UniversityNantong226001China Department of Operation Affiliated Hospital of Nantong UniversityNantong226001China

Objective:Gastric cancer(GC)is a globally common cancer characterized by high incidence and mortality *** in the molecular understanding of GC provide promising targets for GC diagnosis and *** non-coding RNAs(lncRNAs)and their downstream regulators are regarded to be implicated in the progression of multiple types of *** have shown that the lncRNA small nucleolar RNA host gene 4(SNHG4)serves as a tumor promoter in various malignancies,while its function in GC has yet to be ***,our study aimed to explore the role and underlying mechanism of SNHG4 in ***:We used qRT-PCR to analyze SNHG4 expression in GC tissues and ***-Meier analysis was used to assess the correlation between SNHG4 expression and the survival rate of GC *** function experiments such as CCK-8,BrdU,colony formation,flow cytometry analysis,and transwell were performed to explore the effects of SNHG4 on GC cell proliferation,apoptosis,cell cycle,migration,and *** also established xenograft mouse models to explore the effect of SNHG4 on GC tumor ***,dual luciferase reporter assay was used to verify the interaction between SNHG4 and miR-409-3p and between miR-409-3p and cAMP responsive element binding protein 1(CREB1).Results:The results indicated that SNHG4 was overexpressed in GC tissues and cell lines,and was linked with poor survival rate of GC ***4 promoted GC cell proliferation,migration,and invasion while inhibiting cell apoptosis and cell cycle arrest in *** in vivo experiment indicated that SNHG4 facilitated GC tumor ***,SNHG4 was demonstrated to bind to ***,CREB1 was directly targeted by *** assays demonstrated that miR-409-3p deficiency reversed the suppressive impact of SNHG4 knockdown on GC cell ***,miR-409-3p was also revealed to inhibit GC cell proliferation,migration,and invasion by targeting CREB1

关键词： Gastric cancer Small nucleolar RNA host gene 4(SNHG4) MicroRNA-409-3p(miR-409-3p) cAMP responsive element binding protein 1(CREB1)

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