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Chinese Medical Journal 2024年第4期137卷 484-486页

作者： Jian Wu Qi Zhang Xu Jia Yingting Zhu Zhidong Li Shu Tu Ling Zhao Yifan Du Wei Liu Jiaoyan Ren Liangzhi Xu Hanxiang Yu Fagao Luo Wenru Su Ningli Wang yehong zhuo State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterGuangdong Provincial Key Laboratory of Ophthalmology and Visual ScienceSun Yat-sen UniversityGuangzhouGuangdong 510060China Beijing Institute of Ophthalmology Beijing Tongren Eye CenterBeijing Tongren HospitalCapital Medical UniversityBeijing Ophthalmology&Visual Sciences Key LaboratoryBeijing 100730China School of Food Sciences and Engineering South China University of TechnologyGuangzhouGuangdong 510641China Guangzhou Huazhen Biosciences GuangzhouGuangdong 510920China

To the Editor:Normal-tension glaucoma(NTG)is a progressive optic neuropathy characterized by irreversible blindness with statistically normal intraocular pressure(IOP)(≤21 mmHg),^([1,2])which makes early-stage diagnosis in the clinical practice difficult.Pathophysiological pathways underlying NTG include vascular dysregulation,toxic metabolite accumulation,and elevated translaminar cribrosa pressure difference(TLCPD).^([3])Although various small animal models have provided evidence of different pathophysiological mechanisms,the complicated clinical phenotype cannot be fully imitated by secondary disease models,and the gap between rodents and humans weakens their translational potential.Thus,the pathological changes associated with NTG remain unclear.

关键词： elevated glaucoma diagnosis

Selective deletion of zinc transporter 3 in amacrine cells promotes retinal ganglion cell survival and optic nerve regeneration after injury

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Neural Regeneration Research 2023年第12期18卷 2773-2780页

作者： Zhe Liu Jingfei Xue Canying Liu Jiahui Tang Siting Wu Jicheng Lin Jiaxu Han Qi Zhang Caiqing Wu Haishun Huang Ling Zhao yehong zhuo Yiqing Li State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangdong Provincial Key Laboratory of Ophthalmology and Visual ScienceGuangzhouGuangdong ProvinceChina

Vision depends on accurate signal conduction from the retina to the brain through the optic nerve,an important part of the central nervous system that consists of bundles of axons originating from retinal ganglion cells.The mammalian optic nerve,an important part of the central nervous system,cannot regenerate once it is injured,leading to permanent vision loss.To date,there is no clinical treatment that can regenerate the optic nerve and restore vision.Our previous study found that the mobile zinc(Zn^(2+))level increased rapidly after optic nerve injury in the retina,specifically in the vesicles of the inner plexiform layer.Furthermore,chelating Zn^(2+)significantly promoted axonal regeneration with a long-term effect.In this study,we conditionally knocked out zinc transporter 3(ZnT3)in amacrine cells or retinal ganglion cells to construct two transgenic mouse lines(VGAT^(Cre)ZnT3^(fl/fl)and VGLUT2^(Cre)ZnT3^(fl/fl),respectively).We obtained direct evidence that the rapidly increased mobile Zn^(2+)in response to injury was from amacrine cells.We also found that selective deletion of ZnT3 in amacrine cells promoted retinal ganglion cell survival and axonal regeneration after optic nerve crush injury,improved retinal ganglion cell function,and promoted vision recovery.Sequencing analysis of reginal ganglion cells revealed that inhibiting the release of presynaptic Zn^(2+)affected the transcription of key genes related to the survival of retinal ganglion cells in postsynaptic neurons,regulated the synaptic connection between amacrine cells and retinal ganglion cells,and affected the fate of retinal ganglion cells.These results suggest that amacrine cells release Zn^(2+)to trigger transcriptomic changes related to neuronal growth and survival in reginal ganglion cells,thereby influencing the synaptic plasticity of retinal networks.These results make the theory of zinc-dependent retinal ganglion cell death more accurate and complete and provide new insights into the complex interactions between retinal cell networks.

关键词： axonal regeneration conditional knockout neurotransmitter optic nerve injury presynaptic neuron retinal network synaptic connection synaptic vesicles visual acuity zinc transporter 3

Identification and validation of key biomarkers and potential therapeutic targets for primary open-angle glaucoma

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Science China(Life Sciences) 2023年第12期66卷 2837-2850页

作者： Jian Wu Caixia Lin Chenlong Yang Lijie Pan Hongyi Liu Sirui Zhu Shuwen Wei Xu Jia Qi Zhang Ziyu Yu Xiaofang Zhao Weihai Liu yehong zhuo Ningli Wang Beijing Institute of Ophthalmology Beijing Tongren Eye CenterBeijing Tongren HospitalCapital Medical UniversityBeijing Key Laboratory of Ophthalmology and Visual SciencesBeijing 100730China Beijing Tongren Eye Center Beijing Tongren HospitalCapital Medical UniversityBeijing 100730China Department of Neurosurgery Peking University Third HospitalCenter for Precision Neurosurgery and Oncology of Peking University Health Science CenterBeijing 100191China North America Medical Education Foundation Union City CA 94539USA State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangzhou 510060China Spencer Center for Vision Research Byers Eye InstituteSchool of MedicineStanford UniversityPalo Alto CA 94304USA

Primary open-angle glaucoma(POAG)is a prevalent cause of blindness worldwide,resulting in degeneration of retinal ganglion cells and permanent damage to the optic nerve.However,the underlying pathogenetic mechanisms of POAG are currently indistinct,and there has been no effective nonsurgical treatment regimen.The objective of this study is to identify novel biomarkers and potential therapeutic targets for POAG.The mRNA expression microarray datasets GSE27276 and GSE138125,as well as the single-cell high-throughput RNA sequencing(sc RNA-seq)dataset GSE148371 were utilized to screen POAGrelated differentially expressed genes(DEGs).Functional enrichment analyses,protein-protein interaction(PPI)analysis,and weighted gene co-expression network analysis(WGCNA)of the DEGs were performed.Subsequently,the hub genes were validated at a single-cell level,where trabecular cells were annotated,and the mRNA expression levels of target genes in different cell clusters were analyzed.Immunofluorescence and quantitative real-time PCR(q PCR)were performed for further validation.DEGs analysis identified 43 downregulated and 32 upregulated genes in POAG,which were mainly enriched in immune-related pathways,oxidative stress,and endoplasmic reticulum(ER)stress.PPI networks showed that FN1 and DUSP1 were the central hub nodes,while GPX3 and VAV3 were screened out as hub genes through WGCNA and subsequently validated by q PCR.Finally,FN1,GPX3,and VAV3 were determined to be pivotal core genes via single-cell validation.The relevant biomarkers involved in the pathogenesis of POAG,may serve as potential therapeutic targets.Further studies are necessary to unveil the mechanisms underlying the expression variations of these genes in POAG.

关键词： primary open-angle glaucoma biomarkers immune response oxidative stress endoplasmic reticulum stress

Corrigendum to‘microRNA-21a-5p/PDCD4 axis regulates mesenchymal stem cell-induced neuroprotection in acute glaucoma’

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Journal of Molecular Cell Biology 2023年第4期15卷 70-71页

作者： Wenru Su Zuohong Li Yu Jia Yingting Zhu Wenjia Cai Peixing Wan Yingying Zhang Song Guo Zheng yehong zhuo State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangzhou 510060China Department of Clinic Immunology The Third Affiliated Hospital at Sun Yat-sen UniversityGuangzhou 510630China Division of Rheumatology Department of MedicinePenn State Milton S.Hershey Medical CenterHersheyPA 17033USA

Journal of Molecular Cell Biology(2017),9(4),289‒301,https://***/10.1093/jmcb/mjx022 In this article,there were errors in Figures 4D(western blotting analysis),3E and F,5G and H,and 5J and K(flow cytometry analysis).Iba1 band in Figure 4D was the same as Cleaved caspase-3 band in Figure 2F.This was caused by carelessly mistaking the latter(Cleaved caspase-3,#1)for Iba1 band due to the high similarity between images(raw blot images of these bands from three experiments are provided in the Supplementary material).The corrected Figure 4D with updated Iba1 band(#1)and quantitation graph is shown below.

关键词： analysis PDCD4 Figure

PLSCR1 promotes apoptosis and clearance of retinal ganglion cells in glaucoma pathogenesis

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Genes & Diseases 2023年第4期10卷 1564-1581页

作者： Jingyi Luo Qing Lian Deliang Zhu Minglei Zhao Tingfang Mei Bizhi Shang Zeqiu Yang Chujun Liu Wenchang Xu Lan Zhou Keling Wu Xinqi Liu Yuhua Lai Fuxiang Mao Weihua Li Chengguo Zuo Kang Zhang Mingkai Lin yehong zhuo Yizhi Liu Lin Lu Ling Zhao State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangdong Provincial Key Laboratory of Ophthalmology and Visual ScienceGuangzhouGuangdong 510060China Dongguan Guangming Ophthalmic Hospital Dongguan523000China Guangdong Provincial People’s Hospital Guangdong Academy of Medical SciencesGuangzhouGuangdong 510080China Department of Ophthalmology Huizhou Municipal Central HospitalHuizhouGuangdong 516000China Guangdong Province Key Laboratory of Brain Function and Disease Zhongshan School of MedicineSun Yat-sen UniversityGuangzhouGuangdong 510000China Center for Biomedicine and Innovations Faculty of MedicineMacao University of Science and TechnologyMacao 999078China Guangzhou Regenerative Medicine and Health Guangdong Laboratory GuangzhouGuangdong 510530China Research Unit of Ocular Development and Regeneration Chinese Academy of Medical SciencesGuangzhouGuangdong 510060China

Glaucoma is the leading cause of irreversible blindness worldwide.In the pathogen-esis of glaucoma,activated microglia can lead to retinal ganglion cells(RGCs)apoptosis and death,however,the molecular mechanisms remain largely unknown.We demonstrate that phospholipid scramblase 1(PLSCR1)is a key regulator promoting RGCs apoptosis and their clearance by microglia.As evidenced in retinal progenitor cells and RGCs of the acute ocular hypertension(AOH)mouse model,overexpressed PLSCR1 induced its translocation from the nucleus to the cytoplasm and cytomembrane,as well as elevated phosphatidylserine exposure and reactive oxygen species generation with subsequent RGCs apoptosis and death.These damages were effectively attenuated by PLSCR1 inhibition.In the AOH model,PLSCR1 led to an increase in M1 type microglia activation and retinal neuroinflammation.Upregulation of PLSCR1 resulted in strongly elevated phagocytosis of apoptotic RGCs by activated microglia.Taken together,our study provides important insights linking activated microglia to RGCs death in the glaucoma pathogenesis and other RGC-related neurodegenerative diseases.

关键词： Apoptosis Glaucoma Phagocytosis PLSCR1 Retinal ganglion cells

microRNA-21a-5p/PDCD4 axis regulates mesenchymal stem cell-induced neuroprotection in acute glaucoma

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Journal of Molecular Cell Biology 2017年第4期9卷 289-301页

作者： Wenru Su Zuohong Li Yu Jia Yingting Zhu Wenjia Cai Peixing Wan Yingying Zhang Song Guo Zheng yehong zhuo State Key Laboratory of Ophthalmology Zhongshan Ophthalmic Center Sun Yat-sen University Guangzhou 510060 China Department of Clinic Immunology The Third Affiliated Hospital at Sun Yat-sen University Guangzhou 510630 China Division of Rheumatology Department of Medicine Penn State Milton S. Hershey Medical Center Hershey PA 17033 USA

间充质的干细胞(MSC ) 被表明了为许多神经病学的疾病有有希望的治疗学的好处；然而，内在的机制糟糕被理解。这里，我们显示出 MSC 的那 intravitreal 注入在尖锐绿内障的一个老鼠模型的支持的网膜的中心细胞(RGC ) 幸存与 microglial... 详细信息

间充质的干细胞(MSC ) 被表明了为许多神经病学的疾病有有希望的治疗学的好处；然而，内在的机制糟糕被理解。这里，我们显示出 MSC 的那 intravitreal 注入在尖锐绿内障的一个老鼠模型的支持的网膜的中心细胞(RGC ) 幸存与 microglial 激活的重要抑制， TNF- ， IL-1，和反应的氧种类的生产，以及 caspase-8 和 caspase-3 激活。在 vitro， MSC 禁止了两 caspase-8-mediated RGC apoptosis 和 microglial 激活，部分经由 stanniocalcin 的行动 1 (STC1 ) 。而且，我们发现了那 microRNA-21a-5p (miR-21 ) 和它的目标， PDCD4，为 STC1 生产和在 vitro 并且在 vivo 的 MSC 的 neuroprotective 性质是必要的。重要地， miR-21 overexpression 或 PDCD4 尖锐绿内障上的击倒的扩充调停 MSC 的 neuroprotective 效果。miR-21/PDCD4 轴由导致 MSC 分泌施加 neuroprotective 的 STC1 和另外的因素的以前未被认出的 neuroprotective 机制完成的这些数据热点。因此， modulating miR-21/PDCD4 轴可能是为尖锐绿内障和另外的神经病学的疾病的临床的处理的有希望的策略。

关键词：骨髓间充质干细胞神经保护作用青光眼急性诱导 caspase-3 视网膜神经节细胞 strategy

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人工智能在青光眼防治中的应用潜力和方向

中华眼科杂志 2023年第9期59卷 691-695页

作者：卓业鸿吴建中山大学中山眼科中心、眼科学国家重点实验室、广东省眼科视觉科学重点实验室、广东省眼部疾病临床医学研究中心广州510060

人工智能技术的快速迭代推动了眼科智能医学的发展,尤其在以青光眼为首的难治性致盲眼病领域,主要体现在筛查、精准诊断、病程预测等方面。本文从基于人工智能提高青光眼的筛查和诊疗水平、促进人工智能在青光眼防治中应用的举措、青光... 详细信息

人工智能技术的快速迭代推动了眼科智能医学的发展,尤其在以青光眼为首的难治性致盲眼病领域,主要体现在筛查、精准诊断、病程预测等方面。本文从基于人工智能提高青光眼的筛查和诊疗水平、促进人工智能在青光眼防治中应用的举措、青光眼防治中人工智能的应用前景3个方面,阐述人工智能在青光眼防治中的应用潜力和方向,以期和眼科同道共同探讨和发挥人工智能的应用价值。

关键词：青光眼人工智能机器学习深度学习

Validation of glaucoma-like features in the rat episcleral vein cauterization model

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Chinese Medical Journal 2014年第2期127卷 359-364页

作者： Bai Yujing Zhu Yingting Chen Qin Xu Jing Marinko V. Sarunic Uri H. Saragovi zhuo yehong Key Laboratory of Vision Loss and Restoration Ministry of Education Department of Ophthalmology Peking University People's Hospital Beijing 100044 China State Key Laboratory of Ophthalmology Zhongshan Ophthalmic Center Sun Yat-sen University Guangzhou Guangdong 510060 China Department of Ophthalmology First'Affiliated Hospital of Nanjing Medical University Nanjing Jiangsu 210029 China Engineering Science Simon Fraser University Burnaby BC Canada V5A 1S6 Department of Pharmacology and Therapeutics Department of Oncology and the Cancer Center Bloomfield Centre for Research in Aging Lady Davis Institute-Jewish General Hospital McGill University Montreal Quebec Canada H3T 1E2

Background Glaucoma,an irreversible optic nerve neuropathy,always results in blindness.This study aimed to evaluate glaucoma-like features in the rat episcleral vein cauterization （EVC） model by multiple in vivo and in vitro evidences.Methods Wistar rat was used in this study.The elevated intraocular pressure （IOP） was induced by cauterization of three episcleral veins.lOP was monitored with Tono-Pen XL tonometer.Time-dependent changes to the neuronal retinal layers were quantified by Fourier domain-optical coherence tomography.The function of retina was evaluated by electroretinogram （ERG）.Survival of retinal ganglion cells （RGCs） was quantified by retrograde labeling.Histology study was performed with retinal sections stained with hematoxylin-eosin,glial fibrillary acidic protein,and neuronal nuclear antigen.Retina and aqueous humor protein were extracted and cytotoxic protein tumor necrosis factor alpha （TNF-α） and alpha-2 macroglobulin （α2m） were measured with Western blotting.Results EVC is a relatively facile intervention,with low failure rates （＜5％）.After surgical intervention,chronic mild lOP elevation （about 1.6-fold over normal,P ＜0.05） was induced for at least 6 weeks without requiring a second intervention.High lOP causes chronic and progressive loss of RGCs （averaging about 4％ per week）,progressive thinning of neuronal retinal layers （3-5 μm per week）,and reduction of a-and b-wave in ERG.EVC method can also induce glial cell activation and alterations of inflammation proteins,such as TNF-α and α2m.Conclusion EVC method can establish a robust,reliable,economic and highly reproducible glaucomatous animal model.

关键词： glaucoma experimental animal model retinal neurons rat

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剥脱性青光眼发病机制的研究进展

中华眼科杂志 2022年第1期58卷 58-62页

作者：关洁莹朱颖婷卓业鸿中山大学中山眼科中心眼科学国家重点实验室广州510060

剥脱性青光眼是继发于剥脱综合征的一种青光眼类型。近年来,国内外学者对剥脱性青光眼的发病机制和危险因素进行了大量研究,发现了多种危险基因、异常表达的生长因子和细胞因子以及广泛的眼前、后节改变。剥脱性青光眼与全身疾病和环境... 详细信息

剥脱性青光眼是继发于剥脱综合征的一种青光眼类型。近年来,国内外学者对剥脱性青光眼的发病机制和危险因素进行了大量研究,发现了多种危险基因、异常表达的生长因子和细胞因子以及广泛的眼前、后节改变。剥脱性青光眼与全身疾病和环境存在一定关联。本文在对这些成果进行系统总结的基础上,指出当前亟待进行深入研究的问题,以期为该领域提供参考。

关键词：青光眼危险因素假性剥脱综合征发病机制

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关注免疫炎症调控在青光眼全程诊疗中的作用

中华实验眼科杂志 2022年第4期40卷 289-293页

作者：吴建苏文如卓业鸿中山大学中山眼科中心眼科学国家重点实验室广州510060

青光眼是由于病理性眼压升高引起视神经结构和功能损害而导致的不可逆性致盲眼病,具有多因性、强异质性的特点,长期以来降低并控制眼压以减轻视神经损害是青光眼主要的治疗策略。然而,临床实践中发现,尽管部分青光眼患者眼压得到控制,... 详细信息

青光眼是由于病理性眼压升高引起视神经结构和功能损害而导致的不可逆性致盲眼病,具有多因性、强异质性的特点,长期以来降低并控制眼压以减轻视神经损害是青光眼主要的治疗策略。然而,临床实践中发现,尽管部分青光眼患者眼压得到控制,但视神经损害仍持续进展,因此非眼压依赖性的继发视神经损害仍为青光眼发生和发展病理机制中亟需解决的瓶颈问题和研究热点。随着分子生物学研究技术的不断进步,医学基础研究领域已取得突破性进展,青光眼患者缓解局部免疫炎症反应可促进部分视觉恢复。但是由于目前尚无标志性临床应用成果,因此重视基础和临床研究相结合,促进成果转化为目前当务之急。研究者及眼科医师应从青光眼-免疫炎症理论出发理解眼部免疫稳态的重要性,关注眼脑之间生理病理过程的相互关联及全视路的病程进展,充分理解和有效利用新的生物学研究技术为青光眼致病机制研究带来的机遇与启示,进而为全临床青光眼诊疗方案的制定提供指导作用。

关键词：青光眼视神经损伤视网膜神经节细胞免疫调节