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Sonication and grinding pre-treatments on Gelidium amansii seaweed for the extraction and characterization of Agarose

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Frontiers of Environmental Science & Engineering 2018年第4期12卷 17-23页

作者： Kit Wayne Chew Pau Loke Show yee jiun yap Joon Ching Juan Siew Moi Phang Tau Chuan Ling Jo-Shu Chang Department of Chemical and Environmental Engineering Faculty of Engineering University of Nottingham Malaysia Campus Jalan Broga 43500 Semenyih Selangor Darul Ehsan Malaysia Bioseparation Research Group Faculty of Engineering University of Nottingham Malaysia Campus Jalan Broga 43500 Semenyih Selangor Darul Ehsan Malaysia Department of Applied Mathematics Faculty of Engineering University of Nottingham Malaysia Campus Jalan Broga 43500 Semenyih Selangor Darul Ehsan Malaysia Nanotechnology & Catalysis Research Centre (NANOCAT) Institute of Postgraduate Studies University of Malaya 50603 Kuala Lumpur Malaysia Institute of Ocean and Earth Sciences University of Malaya 50603 Kuala Lumpur Malaysia Institute of Biological Sciences University of Malaya 50603 Kuala Lumpur Malaysia Department of Chemical Engineering National Cheng Kung University Tainan 701 Taiwan China

Various prctreatments methods including sonication and grinding were perfonned on red seaweed Gelidium amansii for the subsequent extraction ofagarose. The agarose products are usually extracted from agar powder products from seaweeds. In this study, the agarose was extracted using a direct polyethylene glycol （PEG） method without the need to first process the agar from seaweed. The agar extract was frozen then thawed and mixed directly with PEG solution to precipitate the agarose. The quality of agarose obtained was evaluated through physico-chemical properties analysis which includes spectral technique （FTIR）, melting and boiling point, gel strength and sulfate content. These properties were compared with a non-pretreated sample and it was found that the addition of pretreatment steps improved the quality of agarosc but gave a slightly lower yield. The gel strength of pretreated samples was much higher and the sulfate content was lower compared to non-pretreated samples. The best pretreatment method was sonication which gave gel strength of 742 gcm - and sulfate content of 0.63%. The extraction of agarose can be further improved with the use of different neutralizing agents. Pretreating the seaweed shows potential in improving the quality of agarosc from seaweed and can be applied for thture extraction of the agarose.

关键词： AgaroseGe lidium Pretreatment Seaweed Sonication

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Artificial Intelligence Based MPPT Techniques for Solar Power System: A review

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Journal of Modern Power Systems and Clean Energy 2020年第6期8卷 1043-1059页

作者： Kah Yung yap Charles R.Sarimuthu Joanne Mun-yee Lim the Department of Electrical and Computer Systems Engineering(ECSE) School of EngineeringMonash UniversitySubang JayaMalaysia

In the last decade,artificial intelligence(AI)techniques have been extensively used for maximum power point tracking(MPPT)in the solar power *** is because conventional MPPT techniques are incapable of tracking the global maximum power point(GMPP)under partial shading condition(PSC).The output curve of the power versus voltage for a solar panel has only one GMPP and multiple local maximum power points(MPPs).The integration of AI in MPPT is crucial to guarantee the tracking of GMPP while increasing the overall efficiency and performance of *** selection of AI-based MPPT techniques is complicated because each technique has its own merits and *** general,all of the AI-based MPPT techniques exhibit fast convergence speed,less steady-state oscillation and high efficiency,compared with the conventional MPPT ***,the AI-based MPPT techniques are computationally intensive and costly to ***,the hybrid MPPT is favorable in terms of the balance between performance and complexity,and it combines the advantages of conventional and AI-based MPPT *** this paper,a detailed comparison of classification and performance between 6 major AI-based MPPT techniques have been made based on the review and MATLAB/Simulink simulation *** merits,open issues and technical implementations of AI-based MPPT techniques are *** intend to provide new insights into the choice of optimal AI-based MPPT techniques.

关键词： Maximum power point tracking(MPPT) artificial intelligence(AI) fuzzy logic control(FLC) artificial neural network(ANN) genetic algorithm(GA) swarm intelligence(SI) machine learning(ML)

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Development of a system configuration for a solar powered hydrogen facility using fuzzy logic control

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2013年第11期14卷 822-834页

作者： Ying-tai LOONG Mahidzal DAHARI Hwa-jen yap Hue-yee CHONG Department of Mechanical Engineering Faculty of Engineering University MalayaKuala Lumpur 50603 Malaysia

Solar energy is a natural resource which can be harnessed to provide clean electricity for hydrogen production ***, this technology is not widely used because of control issues, particularly for hydrogen refuelling stations. At present,direct or DC-DC converter couplings are the most common system configurations for hydrogen refuelling stations. However, these system configurations are costly and suffer from gas shortage at hydrogen refuelling stations. Furthermore, the hydrogen produced by such system configurations varies considerably depending on the levels of solar radiation. In order to address these issues, a new system configuration is proposed, incorporating the feedback signal of the storage level in the control system. The photovoltaic(PV) system, electrolyzer, and storage tank are integrated with a fuzzy logic controller(FLC) to determine the backup current compensation for electrolyzer operation in order to obtain the minimum power required for hydrogen production. The proposed FLC is constructed with three input variables which are the PV current, hydrogen storage level, and the battery state of charge. The rules-based fuzzy inference process is based on the proposed configuration which combines the advantages of direct and DC-DC converter coupling configurations. The simulation results show that the proposed configuration offers better adaptability to variable radiation conditions compared to other methods. This gives a more promising option for ensuring the adequacy of hydrogen supply at hydrogen refuelling stations.

关键词： Hydrogen energy Solar energy Hydrogen refueling facility Fuzzy logic

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Fuzzy-based risk prioritization for a hydrogen refueling facility in Malaysia

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2013年第8期14卷 834-834页

作者： Hue-yee Chong Mahidzal Dahari Hwa-jen yap Ying-tai Loong Department of Mechanical Engineering Faculty of Engineering University Malaya

Hydrogen is starting to be mentioned as an alternative fuel to replace the fossil fuel in future transportation applications due to its characteristics of zero greenhouse gas emission and high energy efficiency. Before hydrogen fuel and its facilities can be introduced to the public, relevant safety issues and its hazards must be assessed in order to avoid any chance of injury or loss. While a traditional risk assessment has difficulty in prioritizing the risk of failure modes, this paper proposes a new fuzzy-based risk evaluation technique which uses fuzzy value to prioritize the risk of various scenarios. In this study, the final risk of each failure modes was prioritized by using the MATLAB fuzzy logic tool box with a combination of two assessments. The first assessment was concerned with the criteria which affected the actual probability of *** assessment considered the availability of the standard that was applied to prevent the likelihood of the scenario occurring. On the other hand, the second assessment was focused on evaluating the consequence of the failure by taking into account the availability of detection and the complexity of the failure rather than only the severity of the scenarios. A total of 87 failure scenarios were identified using failure modes and effect analysis(FMEA) procedures on hydrogen refueling station models. Fuzzy-based assessments were performed through risk prioritizing various failure scenarios with a fuzzy value(0 to 1) and risk level(low, medium, and high)while a traditional risk assessment approach presented the risks only in forms of level(low, medium, and/or high). Availability of the fuzzy value enabled further prioritizing on the risk results that fell in the same level of risk. This study concluded that fuzzy-based risk evaluation is able to further prioritize the decisions when compared with a traditional risk assessment method.

关键词：风险评估方法模糊值加氢站马来西亚设施优先级模糊逻辑工具箱故障模式

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Comparative analysis of current diagnostic PCR assays in detecting pathogenic Leptospira isolates from environmental samples

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Asian Pacific Journal of Tropical Medicine 2019年第10期12卷 472-478页

作者： May-Ling yap Zamberi Sekawi Hui-yee Chee Han Kiat Alan Ong Vasantha Kumari Neela Department of Medical Microbiology and Parasitology Faculty of Medicine and Health SciencesUniversiti Putra Malaysia43400 SerdangSelangorMalaysia Department of Pre-Clinical Sciences Faculty of Medicine and Health SciencesUniversiti Tunku Abdul RahmanBandar Sungai Long43000 KajangSelangorMalaysia

Objective:To compare the efficiency of routine diagnostic PCR assays in detecting pathogenic Leptospira isolated from water and ***:Seven routine assays targeting six genes(lip L32,fla B,gyr B,lfb1,sec Y and lig B)were evaluated and compared on the cultures of two groups of pathogenic Leptospira from different *** group included 19 described reference strains recovered from infected human or animals,and another group included 22 environmental isolates from recreational and residential sites in *** latter have been confirmed for presence of pathogenic Leptospira *** positivity or detection sensitivity of each assay was determined and compared between the two ***:Validation on reference strains showed 100.0%PCR sensitivity for all assays except lig B-PCR(95.0%)that failed to amplify Leptospira interrogans serovar *** marked contrast,there was a notable decline in sensitivity in the environmental isolates(lip L32-PCR,95.5%;fla B-PCR,90.9%;gyr B-PCR,77.3%;lfb1-PCR,59.1%;sec Y-PCRs,40.9%G1/G2-PCR,36.4%;lig B-PCR,13.6%),implying a large genetic distance between the two groups,as well as nucleotide polymorphism among environmental ***:High proportion of false-negative PCR results suggests a need of prudent selection of primers in detecting environmental pathogenic *** findings offer valuable insights on the extensive biodiversity of genus Leptospira and its impact on the efficacy and development of molecular detection tool.

关键词： Leptospira Pathogenic species Environmental samples PCR Sensitivity

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Surface modification and functionalization by electrical discharge coating:a comprehensive review

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International Journal of Extreme Manufacturing 2020年第1期2卷 68-99页

作者： Pay Jun Liew Ching yee yap Jingsi Wang Tianfeng Zhou Jiwang Yan Fakulti Kejuruteraan Pembuatan Universiti Teknikal Malaysia MelakaHang Tuah Jaya76100Durian TunggalMelakaMalaysia Marine Engineering College Dalian Maritime University1 Linghai RoadGanjingzi DistrictDalian 116026People’s Republic of China Key Laboratory of Fundamental Science for Advanced Machining Beijing Institute of TechnologyNo.5 Zhongguancun South StreetHaidian DistrictBeijing 100081People’s Republic of China Department of Mechanical Engineering Faculty of Science and TechnologyKeio UniversityHiyoshi 3-14-1Kohoku-kuYokohama 223-8522Japan

Hard coatings are extensively required in industry for protecting mechanical/structural parts that withstand extremely high temperature,stress,chemical corrosion,and other hostile *** discharge coating(EDC)is an emerging surface modification technology to produce such hard coatings by using electrical discharges to coat a layer of material on workpiece surface to modify and enhance the surface characteristics or create new surface *** paper presents a comprehensive overview of EDC technologies for various materials,and summarises the types and key parameters of EDC processes as well as the characteristics of resulting *** provides a systematic summary of the fundamentals and key features of the EDC processes,as well as its applications and future trends.

关键词： electrical discharge coating(EDC) surface modification material characteristics mechanical properties extreme conditions

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弹性湍流及其对微混合效率影响研究的若干进展

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力学与实践 2008年第5期30卷 1-8,15页

作者：杨攀 Lam yee Cheong 朱克勤 Gan Hiong yap 清华大学航天航空学院北京100084 南洋理工大学机械与宇航工程学院

介绍和评述弹性湍流的产生及其对于微混合效率的影响等问题上的若干研究进展.弹性湍流和惯性湍流具有类似的流场特征,但引发机理有所不同.惯性湍流产生的原因是惯性引起的Reynolds应力,而弹性湍流则是由弹性应力所引起的.鉴于在微流动中... 详细信息

介绍和评述弹性湍流的产生及其对于微混合效率的影响等问题上的若干研究进展.弹性湍流和惯性湍流具有类似的流场特征,但引发机理有所不同.惯性湍流产生的原因是惯性引起的Reynolds应力,而弹性湍流则是由弹性应力所引起的.鉴于在微流动中,惯性力可忽略不计,因此牛顿流体的混合变得十分困难.此时可在流体中加入微量高分子聚合物以生成黏弹性流体.由黏弹性流体所引发的弹性湍流在提高流体微混合的效率上可发挥重要作用.

关键词：弹性湍流微混合 Batchelor子区 Weissenberg数 Deborah数

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Wax Removal in Textile Manufacturing Wastewater Using Surface Modification of Rice Straw

Wax Removal in Textile Manufacturing Wastewater Using Surfac...

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International Conference on Soft Computing and its Applications(ICSCA’2012);International Conference on Power Electronics,Systems and Applications(ICPESA’2012)International Conference on Mechanical,Nanotechnology and Cryogenics Engineering(ICMNC’2012);International Conference on Arts and Business Information Systems(ICAEBIS’2012);International Conference on Chemical,Biological and Medical Sciences(ICCBMS’2012);International Conference on Transport,Environment and Civil Engineering(ICTECE’2012)

作者： Wong yee Ching Nur Atiqah Abdullah Taufiq yap Yun Hin Noor Azlina Ibrahim Faculty of Agro Base Industry Universiti Malaysia Kelantan Jeli Campus Centre of Excellence for Catalysis Science and Technology Faculty of Science Universiti Putra Malaysia

In this study, the effect of modification surface on lignocellulosic adsorbent （rice straws） was developed and examined. The surface of lignocellulosic adsorbent was treated with Cetylpyridinium chloride （CPC） solution, sodium hydroxide （NaOH） solution and mixture of NaOH and CPC solution at 90 ± 5℃ with different chemical treatments method. The surface morphologies of treated and untreated rice straws were observed by Scanning Electron Microscope （SEM）. The treated rice straws adsorb more wax compared to untreated rice straws.

关键词： Modified Rice Straws Wax Adsorbent Wax Removal Wastewater Treatment Textile Industry Waste Effluent

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Rectal arterio-portal fistula: An unusual cause of persistent bleeding per rectum following a proximal spleno-renal shunt

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World Journal of Gastroenterology 2013年第25期19卷 4087-4090页

作者： Hao Yun yap Ser yee Lee Yaw Fui Alexander Chung Kiang Hiong Tay Albert Su-Chong Low Choon Hua Thng Krishnakumar Madhavan Department of General Surgery Singapore General Hospital Department of Surgical Oncology National Cancer Centre Singapore Department of Diagnostic Radiology Singapore General Hospital Department of Oncologic Imaging National Cancer Centre Singapore Department of General Surgery National University Hospital Singapore

Gastrointestinal arterio-venous malformations are a known cause of gastrointestinal bleeding. We present a rare case of persistent rectal bleeding due to a rectal arterio-portal venous fistula in the setting of portal hypertension secondary to portal vein thrombosis. The portal hypertension was initially surgically treated with splenectomy and a proximal splenorenal shunt. However, rectal bleeding persisted even after surgery, presenting us with a diagnostic dilemma. The patient was re-evaluated with a computed tomography mesenteric angiogram which revealed a rectal arterio-portal fistula. Arterio-portal fistulas are a known but rare cause of portal hypertension, and possibly the underlying cause of continued rectal bleeding in this case. This was successfully treated using angiographic localizationand super-selective embolization of the rectal arterioportal venous fistula via the right internal iliac artery. The patient subsequently went on to have a full term pregnancy. Through this case report, we hope to high-light awareness of this unusual condition, discuss the diagnostic workup and our management approach.

关键词： Portal hypertension Esophageal varices Splenorenal shunt Arteriovenous malformations Portal vein thrombosis

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Distinctive serotypes of SARS-related coronaviruses defined by convalescent sera from unvaccinated individuals

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hLife 2023年第1期1卷 26-34页

作者： Chee Wah Tan Feng Zhu Wan Ni Chia #Barnaby EYoung Aileen Ying Yan Yeoh Thomas Althaus Chee Fu Yung Wee Chee yap Beng Lee Lim Mark I-C Chen Jinyan Zhang Yun Yan Mah Eric Voiglio Alex Sigal Jianxin Huo Shengli Xu yee Joo Tan Kong-Peng Lam David Lye Lin-Fa Wang Programme in Emerging Infectious Diseases Duke-NUS Medical SchoolSingapore National Center of Infectious Diseases Singapore Tan Tock Seng Hospital Singapore Lee Kong Chian School of Medicine Nanyang Technology UniversitySingapore Centre Scientifique de Monaco Monaco Direction de I’Action Sanitaire de Monaco Monaco Infectious Disease Service Department of PediatricsKK Women’s and Children’s HospitalSingapore Duke-NUS Medical School Singapore African Health Research Institute DurbanSouth Africa Singapore Immunology Network Agency for ScienceTechnology and ResearchSingapore Department of Physiology Yong Loo Lin School of MedicineNational University of SingaporeSingapore Department of Microbiology and Immunology Yong Loo Lin School of MedicineNational University of SingaporeSingapore Infectious Diseases Translational Research Programme National University of SingaporeSingapore 14School of Biological SciencesNanyang Technological UniversitySingapore Singhealth Duke-NUS Global Health Institute Singapore

Multiple Omicron sub-lineages have emerged,with Omicron XBB and XBB.1.5 subvariants becoming the dominant variants globally at the time of this *** key feature of new variants is their ability to escape humoral immunity despite the fact that there are limited genetic changes from their preceding *** raises the question of whether Omicron should be regarded as a separate serotype from viruses serologically clustered with the ancestral severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)***,we present cross-neutralization data based on a pseudovirus neutralization test using convalescent sera from naïve individuals who had recovered from primary infection by SARS-CoV-1 and SARS-CoV-2 strains/variants including the ancestral virus and variants Beta,Delta,Omicron BA.1,Omicron BA.2 and Omicron *** results revealed no significant cross-neutralization in any of the three-way testing for SARS-CoV-1,ancestral SARS-CoV-2 and SARS-CoV-2 Omicron *** data argue for the assignment of three distinct serotypes for the currently known human-infecting SARS-related coronaviruses.

关键词： SARS-CoV-2 SARS-CoV-1 serotype Omicron primary infection convalescent sera

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