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Immobilized CeO_(2)@C-N Heterogenous Structures with Enhanced Dual Modal Photodynamic/Photothermal Bacterial Inactivation Under NIR Laser Irradiation

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Journal of Analysis and Testing 2023年第3期7卷 189-203页

作者： Yijun Pang Zhiquan Chen Didi Yang xuke Fan Zhenda Liang Yongtao xu Sheng ye Jiancong Liu Li Zhou Institute of Environmental Research at Great Bay Area Key Laboratory for Water Quality and Conservation of the Pearl River DeltaMinistry of EducationGuangzhou UniversityGuangzhou 510006China School of Pharmacy Hubei University of Science and TechnologyXianning 437100China

Non-antibiotic fungicides are urgently needed due to the potential threat of drug-resistant bacteria to human *** this research,a novel antibacterial nanoplatform based on CeO_(2)@C-N has been developed to achieve rapid near-infrared(NIR)laser-induced *** prepared CeO_(2)@C-N hybrid material exhibited a nanowire-like structure,with dispersed CeO_(2)nanoparticles averaging 5 nm in size within the heterogeneous configuration.X-ray photoelectron spectroscopy(XPS)and Raman spectra revealed that the heterogenous structures have a significant amount of oxygen vacancies and ***,CeO_(2)@C-N has a narrower band gap than CeO_(2),which allows for broader absorbance extending to the NIR *** these unique physiochemical properties,CeO_(2)@C-N could inactivate *** and MRSA at a low concentration(20 mg/L)under 808 nm NIR laser(1 W/cm^(2))*** excellent bactericidal activity of CeO_(2)@C-N is attributed to the combination of photodynamic and photo thermal processes,based on its excellent photo-thermal conversion property,detection of reactive oxygen species(ROS)(^(1)O_(2)and·OH)under light irradiation,and scavenger quenching experiment *** study offers a feasible and efficient way to fabricate a highly effective antibacterial candidate.

关键词： Photothermal Photodynamic Heterogenous structure Antibacterial Cerium Carbon

Inhibition of the spread of endophytic Sporisorium reilianum renders maize resistance to head smut

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The Crop Journal 2015年第2期3卷 87-95页

作者： Xianrong Zhao Jianrong ye Lai Wei Nan Zhang Yuexian Xing Weiliang Zuo Qing Chao Guoqing Tan Mingliang xu National Maize Improvement Center of China China Agricultural University Institute of Genetics and Developmental Biology Chinese Academy of Sciences Maize Research Institute Jilin Academy of Agricultural Sciences Institute of Botany Chinese Academy of Sciences

Head smut, caused by the fungal pathogen Sporisorium reilianum, poses a grave threat to maize(Zea mays) production worldwide. Here we report cytological and molecular evidence for maize resistance to head smut. During early stages of root infection, S. reilianum mycelium was capable of penetrating the root epidermis of both resistant(Ji1037) and susceptible(HZ4) inbred lines. S. reilianum hyphae were observed in the root–stem junction at 6 days after inoculation. In an attempt to monitor hyphal spread within the maize plant,a highly specific and sensitive real-time PCR method was established to estimate the hyphal content in infected maize tissues. During the upward growth of endophytic ***, the extent of hyphal spread was markedly different between Ji1037 and HZ4. Very little or no pathogen was detected in aerial parts of Ji1037, whereas large amounts of pathogen accumulated in aboveground tissues, particularly inflorescences, of HZ4. Thus,maize resistance to S. reilianum was achieved mainly by inhibition of endophytic hyphal growth rather than by prevention of early-root penetration by the pathogen.

关键词： Head smut Sporisorium reilianum Maize Resistance

传统中药材全蝎热稳定性多肽的消化酶抗性研究

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湖北医药学院学报 2024年第4期43卷 364-370页

作者：覃陈虎孙芳叶祥东罗旭东陈宗运湖北医药学院基础医学院生物化学教研室湖北医药学院武当特色中药研究湖北省重点实验室湖北十堰442000

目的:研究传统中药材全蝎三种热稳定性多肽BmKcug2、BmKcug2-P1和Martentoxin-P2的消化酶抗性。方法:结合重组表达、消化酶耐受实验、高效液相色谱技术及结构分析,评价多肽BmKcug2、BmKcug2-P1和Martentoxin-P2的肠胃蛋白酶稳定性。结果... 详细信息

目的:研究传统中药材全蝎三种热稳定性多肽BmKcug2、BmKcug2-P1和Martentoxin-P2的消化酶抗性。方法:结合重组表达、消化酶耐受实验、高效液相色谱技术及结构分析,评价多肽BmKcug2、BmKcug2-P1和Martentoxin-P2的肠胃蛋白酶稳定性。结果:多肽BmKcug2对胃蛋白酶、胰蛋白酶、α-胰凝乳蛋白酶都有很好的稳定性;多肽BmKcug2-P1对胃蛋白酶稳定性较好,对胰蛋白酶也具有一定的抗性,但对α-胰凝乳蛋白酶稳定较差;多肽Martentoxin-P2的酶稳定性谱与BmKcug2-P1类似。结论:本研究发现了全蝎毒腺中存在多肽能够耐受肠胃蛋白酶的消化作用,阐明了全蝎毒腺中多肽的消化酶稳定性,为深入研究全蝎中有效药用成分提供了思路,为系统研究全蝎多肽活性成分和口服给药途径之间的关系奠定了基础。

关键词：全蝎多肽肠胃蛋白酶酶稳定性

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Adaptive controller design for upper-limb rehabilitation robot 25th

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Adaptive controller design for upper-limb rehabilitation rob...

气相色谱-负化学离子源质谱法测定中链氯化石蜡3种定量方法比较研究

第25届中国控制与决策会议

作者： Hao-Bo Kang Jian-Hui Wang Li-ye Yu Lei xu the College of Information Science and Engineering Northeastern University the State Key Laboratory of Hybrid Process Industry Automation System and Equipment Technology

ISBN: (纸本)9781467355322

This paper studies the problem of robust output feedback control for a class of upper-limb rehabilitation robot, which can assist with shoulder, elbow and wrist joint movements. The proposed controller can guarantee the robustness of the robotic system in the presence of model uncertainties, as well as the ultimate boundedness of the presented adaptive observerbased controller. An obvious advantage of the proposed method is that the implemented information for feedback is only the measurement of position. Without additional sensors, the information of velocity and acceleration is provided by an adaptive observer. By utilizing a novel adaptive logic-based switching control scheme, a trajectory tracking performance is well achieved with a tunable error bound. Finally, a simulation example verifes the effectiveness of the proposed control scheme.

关键词： adaptive control robust control observerbased control robotics.

来源： cnki会议评论

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浙江化工 2024年第8期55卷 49-54页

作者：蒋武翁振杰张达卢立用许秀兰叶志信温州市质量技术检测科学研究院浙江温州325000

为阐明中链氯化石蜡现行3种定量方法的测定结果差异,配制不同浓度、不同氯含量的标准样品及含有短链氯化石蜡的模拟样品和基质干扰样品,比较研究3种定量方法对不同试样的测定数据。结果表明:校准曲线法和多元线性回归法适用于待测试样... 详细信息

为阐明中链氯化石蜡现行3种定量方法的测定结果差异,配制不同浓度、不同氯含量的标准样品及含有短链氯化石蜡的模拟样品和基质干扰样品,比较研究3种定量方法对不同试样的测定数据。结果表明:校准曲线法和多元线性回归法适用于待测试样氯含量与曲线氯含量相近的样品测定,总体响应因子校正法适用于浓度与曲线浓度相近的待测试样测定。实际检测时,总体响应因子校正法的偏差相对可控,应尽量建立与待测试样氯含量接近的标准曲线使定量结果更加可靠。

关键词：气相色谱-负化学离子源质谱法中链氯化石蜡定量方法

骨髓腔穿刺技术用于创伤性失血休克患者输液通路建立对抢救成功率的影响

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河北医科大学学报 2024年第2期45卷 221-225页

作者：杨玮琦徐海婷黄艳萍虞大为中国人民解放军联勤保障部队第九〇四医院急诊科江苏无锡214000

目的探讨骨髓腔穿刺技术用于创伤性失血休克患者输液通路建立对抢救成功率的影响。方法选取2021年1月—2022年1月40例创伤性失血休克患者,均接受经骨髓腔穿刺建立血管通路,另选骨髓腔穿刺技术开展前(2020年1月—2020年12月)40例创伤性... 详细信息

目的探讨骨髓腔穿刺技术用于创伤性失血休克患者输液通路建立对抢救成功率的影响。方法选取2021年1月—2022年1月40例创伤性失血休克患者,均接受经骨髓腔穿刺建立血管通路,另选骨髓腔穿刺技术开展前(2020年1月—2020年12月)40例创伤性失血休克患者,均接受中心静脉穿刺建立输液通路治疗。观察2组4 h内治疗效果、一次性穿刺成功率、建立输液通路时间、血压回升时间、救治生存率,比较血管通路建立2 h后凝血指标[凝血酶原时间(prothrombin time,PT)、活化部分酶凝血酶时间(activated partial enzyme thrombin time,APTT)、凝血酶时间(thrombin time,TT)]及血气指标[动脉血氧分压(arterial partial pressure of oxygen,PaO_(2))、动脉血二氧化碳分压(partial pressure of arterial blood carbon dioxide,PaCO_(2))、乳酸],观察比较2组不良反应发生率。结果2组性别、年龄、创伤原因比较,差异无统计学意义(P>0.05);观察组有效率显著高于对照组(P<0.05);观察组一次性穿刺成功率、救治生存率占比显著高于对照组,建立输液通路时间、血压回升时间显著低于对照组(P<0.05);治疗后观察组APTT、PT、TT时间显著低于治疗前且低于对照组治疗后(P<0.05);治疗后观察组乳酸、PaCO_(2)水平低于治疗前且低于对照组治疗后,PaO_(2)高于治疗前且高于对照组(P<0.05);观察组不良反应发生率显著低与对照组(P<0.05)。结论利用骨髓腔穿刺技术为创伤性失血休克患者建立血管通路,可提高抢救成功率,且快速有效。

关键词：休克,出血性骨髓穿刺术

人乳头瘤病毒16/18型感染乳腺癌患者病灶核因子κB相互作用的长链非编码RNA表达水平及其与乳腺癌分子分型和预后关系

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肿瘤综合治疗电子杂志 2024年第1期10卷 122-127页

作者：钱烨张燕鲁小敏吉浩明徐榕蔓储建华南通大学附属海安医院肿瘤科江苏南通226600

目的分析人乳头瘤病毒(human papilloma virus,HPV)16/18型感染乳腺癌患者病灶核因子κB相互作用的长链非编码RNA(NF-κB interacting long non-coding RNA,NKILA)表达情况及其与乳腺癌分子分型、预后的相关性。方法选取2016年1月至2... 详细信息

目的分析人乳头瘤病毒(human papilloma virus,HPV)16/18型感染乳腺癌患者病灶核因子κB相互作用的长链非编码RNA(NF-κB interacting long non-coding RNA,NKILA)表达情况及其与乳腺癌分子分型、预后的相关性。方法选取2016年1月至2018年1月南通大学附属海安医院收治的334例乳腺癌患者(观察组)和50例乳腺良性疾病患者(对照组)作为研究对象,对观察组患者肿瘤组织、癌旁组织和对照组患者病灶组织标本进行NKILA表达检测,并检测血清指标水平。分析两组NKILA表达情况与血清学指标的相关性,比较不同HPV分型感染情况、不同分子分型、不同预后患者病灶NKILA表达差异。结果观察组患者肿瘤组织NKILA相对表达量显著低于癌旁组织(P <0.05),癌旁组织NKILA相对表达量显著低于对照组(P <0.05)。HPV 16/18型感染组肿瘤组织NKILA相对表达量、NKILA高表达率均显著低于非HPV16/18型感染组(均P <0.05)。Luminal A型组、Luminal B型组患者肿瘤组织NKILA相对表达量、NKILA高表达率均显著高于人表皮生长因子受体2(human epidermal growth factor receptor-2,HER-2)过表达型组(均P <0.05),HER-2过表达型组肿瘤组织NKILA相对表达量显著高于三阴性组(P <0.05)。复发转移组肿瘤组织NKILA相对表达量、NKILA高表达率均低于未复发转移组(均P <0.05)。NKILA低表达组3年无复发转移率显著低于NKILA高表达组[24.7%(36/146)∶40.0%(58/145),P <0.05]。结论相比乳腺癌旁组织和良性疾病病灶,乳腺癌患者病灶NKILA表达下降,且HPV 16/18型感染者、三阴性乳腺癌者和术后复发转移者病灶NKILA表达下降更为明显,NKILA低表达与患者术后复发转移相关。

关键词：人乳头瘤病毒乳腺癌核因子κB相互作用的长非编码RNA 分子分型预后

Soil Organic Carbon Stocks as Afected by Tillage Systems in a Double-Cropped Rice Field

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Pedosphere 2013年第5期23卷 696-704页

作者： xu Shang-Qi ZHANG Ming-Yuan ZHANG Hai-Lin CHEN Fu YANG Guang-Li XIAO Xiao-ping College of Agronomy and Biotechnology China Agricultural University Key Laboratory of Farming System Ministry of Agriculture of China Soil and Fertilizer Institute of Hunan Province

Tillage practices can potentially affect soil organic carbon （SOC） accumulation in agricultural soils. A 4-year experiment was conducted to identify the influence of tillage practices on SOC sequestration in a double-cropped rice （Oryza sativa L.） field in Hunan Province of China. Three tillage treatments, no-till （NT）, conventional plow tillage （PT）, and rotary tillage （RT）, were laid in a randomized complete block design. Concentrations of SOC and bulk density （BD） of the 0-80 cm soil layer were measured, and SOC stocks of the 0-20 and 0-80 cm soil layers were calculated on an equivalent soil mass （ESM） basis and fixed depth （FD） basis. Soil carbon budget （SCB） under different tillage systems were assessed on the basis of emissions of methane （CH4） and CO2 and the amount of carbon （C） removed by the rice harvest. After four years of experiment, the NT treatment sequestrated more SOC than the other treatments. The SOC stocks in the 0-80 cm layer under NT （on an ESM basis） was as high as 129.32 Mg C ha-1, significantly higher than those under PT and RT （P 〈 0.05）. The order of SOC stocks in the 0-80 cm soil layer was NT 〉 PT 〉 RT, and the same order was observed for SCB; however, in the 0-20 cm soil layer, the RT treatment had a higher SOC stock than the PT treatment. Therefore, when comparing SOC stocks, only considering the top 20 cm of soil would lead to an incomplete evaluation for the tillage-induced effects on SOC stocks and SOC sequestrated in the subsoil layers should also be taken into consideration. The estimation of SOC stocks using the ESM instead of FD method would better reflect the actual changes in SOC stocks in the paddy filed, as the FD method amplified the tillage effects on SOC stocks. This study also indicated that NT plus straw retention on the soil surface was a viable option to increase SOC stocks in paddy soils.

关键词： bulk density equivalent soil mass basis soil carbon budget straw retention

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认知识解许渊冲唐诗英译“意美”的重构

商洛学院学报 2024年第1期38卷 56-63页

作者：李亚萍商洛学院人文学院陕西商洛726000

运用认知识解机制的辖域、详略度、视角和突显四个要素分析了许渊冲唐诗英译文本。研究发现,许渊冲通过补充缺省辖域、互换语言表达的层级范畴、转换人称视角和改变分句主位与述位或在述位中增加新信息的策略,向目的语读者展现出一幅幅... 详细信息

运用认知识解机制的辖域、详略度、视角和突显四个要素分析了许渊冲唐诗英译文本。研究发现,许渊冲通过补充缺省辖域、互换语言表达的层级范畴、转换人称视角和改变分句主位与述位或在述位中增加新信息的策略,向目的语读者展现出一幅幅清晰可见的场景,从而关照其阅读期望,重构了唐诗的意美,同时也说明认知识解译者的翻译策略,有助于揭示译者重构译文过程中复杂的心智路径。

关键词：认知识解意美许渊冲唐诗英译