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基于多主体治理视角的城市更新研究进展与展望

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现代城市研究 2024年第6期39卷 1-7,45页

作者：高雅薇孙伟官卫华中国科学院南京地理与湖泊研究所中国科学院大学南京市城市规划编制研究中心

纵观国内外城市更新历程,其基本遵循政府主导、政企合作、多方参与的更新模式与路径。文章基于我国城市更新治理模式分类,从外部及内生两个层面对其驱动机理构建逻辑框架,在此基础上对3种类型城市更新治理模式的效应研究进行全面梳理总... 详细信息

纵观国内外城市更新历程,其基本遵循政府主导、政企合作、多方参与的更新模式与路径。文章基于我国城市更新治理模式分类,从外部及内生两个层面对其驱动机理构建逻辑框架,在此基础上对3种类型城市更新治理模式的效应研究进行全面梳理总结。研究发现:(1)城市更新治理模式与城市功能迭代融合性研究值得关注;(2)当前更新机理研究对多元主体博弈的制度经济学探索仍显不足;(3)城市更新效应评价研究集中于社会经济效益分析,应加强环境生态文化等综合效应评价;(4)公众参与城市更新的合理性和可行性仍有待深入探讨。

关键词：城市更新治理模式驱动机理效应评估综述

基于转录组数据挖掘外源褪黑激素影响水貂卵巢发育的分子机制

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畜牧兽医学报 2024年第2期55卷 607-618页

作者：高娅薇彭弟孙朝阳晏子越崔凯马泽芳青岛农业大学动物科技学院青岛266109

旨在探究外源褪黑激素(melatonin,MLT)影响水貂卵巢发育的分子机制。本研究随机选取相同饲养条件下、体重相似的200只3月龄健康白色雌性水貂,均分为试验组和对照组。试验组埋植1粒含MLT 18 mg·粒-1的埋植剂,对照组埋植1粒不含MLT的空粒... 详细信息

旨在探究外源褪黑激素(melatonin,MLT)影响水貂卵巢发育的分子机制。本研究随机选取相同饲养条件下、体重相似的200只3月龄健康白色雌性水貂,均分为试验组和对照组。试验组埋植1粒含MLT 18 mg·粒-1的埋植剂,对照组埋植1粒不含MLT的空粒,当水貂处于准备配种期时随机采集试验组和对照组各4只水貂的卵巢,然后采用Trizol法进行总RNA提取,构建cDNA文库,并利用Illumina平台测序后进行生物信息学分析,对差异表达基因进行GO功能分类和KEGG通路注释,寻找MLT调控卵巢发育的相关候选基因和代谢通路。为进一步验证测序数据的可靠性,随机选取6个差异表达基因进行qPCR验证。转录组结果显示,试验组和对照组的SNPs位点均在120000个以上,且均主要出现在基因区,两组的SNP突变类型均为转换型多于颠换型和杂合型;试验组与对照组相比,差异表达基因发生SE事件的数量最多;本试验共获得9631个新基因,其中有1323个、1317个新基因分别注释到TrEMBL和NR数据库,注释比例明显高于其他数据。试验组与对照组相比共发现206个差异表达基因(其中有36个未被注释的基因),69个为上调基因,137个为下调基因;GO功能富集显示,共计7个差异表达基因富集于生殖过程;KEGG通路富集结果显示,共计7个差异表达基因富集于5条与繁殖相关的通路。综合GO功能分类和KEGG通路注释结果分析,共筛选得到4个与MLT影响卵巢发育相关的候选基因,均为上调基因,分别是KRT19、GSTT1、ALAS 1和NR 4A1。本研究通过对MLT埋植和未埋植MLT的准备配种期水貂卵巢进行转录组学分析,挖掘出MLT可能通过调节KRT19、GSTT1、ALAS1、NR 4A1等基因促进卵巢发育,研究结果对水貂的育种工作提供了理论基础。

关键词：水貂褪黑激素转录组卵巢发育差异表达基因

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睡眠剥夺探讨激素水平改变与骨质疏松症相关性研究进展

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中国骨质疏松杂志 2024年第3期30卷 436-440页

作者：谢兴文高亚雄高亚伟李宁林德民闫文马成黄委委刘皓贾斌奎甘肃中医药大学附属医院甘肃兰州730000 甘肃中医药大学甘肃兰州730000

骨质疏松症(Osteoporosis,OP)是一种因骨量减低、骨组织微结构遭到损坏,导致骨脆性增加而易发生骨折为特征的一种代谢性疾病。近年来研究发现睡眠剥夺会在激素水平影响OP进展,特别是生长激素、性激素、甲状腺激素在睡眠剥夺下的变化尤... 详细信息

骨质疏松症(Osteoporosis,OP)是一种因骨量减低、骨组织微结构遭到损坏,导致骨脆性增加而易发生骨折为特征的一种代谢性疾病。近年来研究发现睡眠剥夺会在激素水平影响OP进展,特别是生长激素、性激素、甲状腺激素在睡眠剥夺下的变化尤为明显。故本文就睡眠剥夺下生长激素、性激素、甲状腺激素水平的变化与OP的相关性进行综述,进一步明确“睡眠剥夺⁃激素水平⁃骨质疏松症”三者关系,以期就睡眠角度为临床防治OP提供理论依据。

关键词：睡眠剥夺骨质疏松症生长激素性激素甲状腺激素

基于振动台试验的鱼线固定梅瓶文物响应规律性研究

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振动与冲击 2024年第4期43卷 250-260页

作者：杨维国高雅巍王萌刘佩葛家琪邹晓光北京交通大学土木建筑工程学院北京100044 中国航空规划设计研究总院有限公司北京100120

为了探索鱼线固定梅瓶文物在实际博物馆的地震响应以及抗震效果,首先选取了典型梅瓶文物,并在三层钢筋混凝土框架结构中开展了24种地震工况的振动台试验,然后建立了上述试验所用梅瓶文物的有限元模型,验证了有限元模型的准确性,最后采... 详细信息

为了探索鱼线固定梅瓶文物在实际博物馆的地震响应以及抗震效果,首先选取了典型梅瓶文物,并在三层钢筋混凝土框架结构中开展了24种地震工况的振动台试验,然后建立了上述试验所用梅瓶文物的有限元模型,验证了有限元模型的准确性,最后采用增量动力分析法分析了该文物在两种常见直径鱼线保护措施下的运动响应。结果表明:鱼线固定梅瓶文物在地震作用下会产生滑移、摇摆、倾覆以及鱼线断裂等现象;不同楼层下的文物响应差别较大,尤其在大震作用下,高楼层的鱼线固定梅瓶文物易发生倾覆和鱼线断裂破坏,要重视高楼层文物的震前保护措施;在较强的地震作用下,仅依靠增大鱼线直径有时对控制文物的倾覆情况起不到关键性决定作用,需要进一步采取其它措施对文物进行保护。

关键词：鱼线固定梅瓶文物振动台试验有限元模型运动响应增量动力分析

METTL14 is a chromatin regulator independent of its RNA N^(6)-methyladenosine methyltransferase activity

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Protein & Cell 2023年第9期14卷 683-697页

作者： Xiaoyang Dou Lulu Huang Yu Xiao Chang Liu Yini Li Xinning Zhang Lishan Yu Ran Zhao Lei Yang Chuan Chen Xianbin Yu Boyang gao Meijie Qi yawei gao Bin Shen Shuying Sun Chuan He Jun Liu Department of Chemistry and Institute for Biophysical Dynamics University of ChicagoChicagoIL 60637USA Howard Hughes Medical Institute ChicagoIL 60637USA State Key Laboratory of Protein and Plant Gene Research Peking-Tsinghua Center for Life SciencesSchool of Life SciencesPeking UniversityBeijing 100871China Department of Physiology and Brain Science Institute Johns Hopkins University School of MedicineBaltimoreMD 21205USA Institute for Regenerative Medicine Shanghai East HospitalShanghai Key Laboratory of Signaling and Disease ResearchFrontier Science Center for Stem Cell ResearchSchool of Life Sciences and TechnologyTongji UniversityShanghai 200120China The Institute of Translational Medicine School of MedicineZhejiang UniversityHangzhou 310029China Division of Life Sciences and Medicine Center for Reproductive MedicineThe First Affiliated Hospital of USTCUniversity of Science and Technology of ChinaHefei230000China Clinical and Translational Research Center of Shanghai First Maternity&Infant Hospital Shanghai Key Laboratory of Signaling and Disease ResearchFrontier Science Center for Stem Cell ResearchSchool of Life Sciences and TechnologyTongji UniversityShanghai 200092China State Key Laboratory of Reproductive Medicine Center for Global HealthGusu SchoolWomen's Hospital of Nanjing Medical UniversityNanjing Maternity and Child Health Care HospitalNanjing Medical UniversityNanjing211166China Department of Biochemistry and Molecular Biology University of ChicagoChicagoIL 60637USA

METTL3 and METTL14 are two components that form the core heterodimer of the main RNA m^(6)A methyltransferase complex(MTC)that installs m^(6)***,depletion of METTL3 or METTL14 displayed distinct effects on stemness maintenance of mouse embryonic stem cell(mESC).While comparable global hypo-methylation in RNA m^(6)A was observed in Mettl3 or Mettl14 knockout mESCs,***14 knockout led to a globally decreased nascent RNA synthesis,whereas Mettl3 depletion resulted in transcription upregulation,suggesting that METTL14 might possess an mA-independent role in gene *** found that METTL14 colocalizes with the repressive H3K27me3 ***,METTL14,but not METTL3,binds H3K27me3 and recruits KDM6B to induce H3K27me3 demethylation independent of *** of METTL14 thus led to a global increase in H3K27me3 level along with a global gene *** effects of METTL14 on regulation of H3K27me3 is essential for the transition from self-renewal to differentiation of *** work reveals a regulatory mechanism on heterochromatin by METTL14 in a manner distinct from METTL3 and independently of m^(6)A,and critically impacts transcriptional regulation,stemness maintenance,and differentiation ofmESCs.

关键词： METTL14 chromatin H3K27me3 m^(6)A-independent mESC differentiation

Esrrb plays important roles in maintaining self-renewal of trophoblast stem cells (TSCs) and reprogramming somatic cells to induced TSCs

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Journal of Molecular Cell Biology 2019年第6期11卷 463-473页

作者： Haibo gao Rui gao Linfeng Zhang Wenchao Xiu Ruge Zang Hong Wang Yong Zhang Jiayu Chen yawei gao Shaorong gao Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital Shanghai Key Laboratory of Signaling and Disease Research School of Life Sciences and Technology Tongji University Shanghai 200092 China Peking University-Tsinghua University-National Institute of Biological Sciences Joint Graduate Program School of Life Sciences Tsinghua University Beijing 100084 China

Trophoblast stem cells (TSCs), which can be derived from the trophoectoderm of a blastocyst, have the ability to sustain self-renewal and differentiate into various placental trophoblast cell types. Meanwhile, essential insights into the molecular mechanisms controlling the placental development can be gained by using TSCs as the cell model. Esrrb is a transcription factor that has been shown to play pivotal roles in both embryonic stem cell (ESC) and TSC, but the precise mechanism whereby Esrrb regulates TSC-specific transcriptome during differentiation and reprogramming is still largely unknown. In the present study, we elucidate the function of Esrrb in self-renewal and differentiation of TSCs, as well as during the induced TSC (iTSC) reprogramming. We demonstrate that the precise level of Esrrb is critical for stem state maintenance and further trophoblast differentiation of TSCs, as ectopically expressed Esrrb can partially block the rapid differentiation of TSCs in the absence of fibroblast growth factor 4. However, Esrrb depletion results in downregulation of certain key TSC-specific transcription factors, consequently causing a rapid differentiation of TSCs and these Esrrb-deficient TSCs lose the ability of hemorrhagic lesion formation in vivo. This function of Esrrb is exerted by directly binding and activating a core set of TSC-specific target genes including Cdx2, Eomes, Sox2, Fgfr4, and Bmp4. Furthermore, we show that Esrrb overexpression can facilitate the MEF-to-iTSC conversion. Moreover, Esrrb can substitute for Eomes to generate GEsTM-iTSCs. Thus, our findings provide a better understanding of the molecular mechanism of Esrrb in maintaining TSC self-renewal and during iTSC reprogramming.

关键词： Esrrb trophoblast stem cell self-renwwal differentiation iTSC reprogramming

Contamination trends of polybrominated diphenyl ethers, organochlorine pesticides and heavy metals in sediments from Dagu Drainage River estuary, Tianjin

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Chinese Science Bulletin 2007年第10期52卷 1320-1326页

作者： LU JianXia WANG yawei ZHANG QingHua gao ErLe JIANG GuiBint State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences ChineseAcademy of Sciences Beijing 100085 China

Concentrations of polybrominated diphenyl ethers (PBDEs), organochlorine pesticides (OCPs) and heavy metals in two sediment cores, taken from the Dagu Drainage River, were determined. The contamination trends of these three kinds of compounds were obtained. The results showed that concentrations of PBDEs increased in recent years; there were new contamination sources for OCPs in this area; contamination levels of heavy metals had no significant change in the last several years. Comparing the results of these two cores, erosion and dilution of seawater had different effects on the transportation of these compounds.

关键词：天津大沽河口沉积物多溴联苯醚有机氯农药重金属污染趋势

Correction to:Nuclear m6A reader YTHDC1 regulates the scaffold function of LINE1 RNA in mouse ESCs and early embryos

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Protein & Cell 2022年第6期13卷 470-471页

作者： Chuan Chen Wenqiang Liu Jiayin Guo Yuanyuan Liu Xuelian Liu Jun Liu Xiaoyang Dou Rongrong Le Yixin Huang Chong Li Lingyue Yang Xiaochen Kou Yanhong Zhao You Wu Jiayu Chen Hong Wang Bin Shen yawei gao Shaorong gao Institute for Regenerative Medicine Shanghai East HospitalShanghai Key Laboratory of Signaling and Disease ResearchFrontier Science Center for Stem Cell ResearchSchool of Life Sciences and TechnologyTongji UniversityShanghai 200120China Clinical and Translation Research Center of Shanghai First Maternity&Infant Hospital Shanghai Key Laboratory of Signaling and Disease ResearchFrontier Science Center for Stem Cell ResearchSchool of Life Sciences and TechnologyTongji UniversityShanghai 200092China State Key Laboratory of Reproductive Medicine Department of Prenatal DiagnosisWomen’s Hospital of Nanjing Medical UniversityNanjing Maternity and Child Health Care HospitalNanjing Medical UniversityNanjing 211166China School of Life Sciences Peking UniversityBeijing 100871China Peking-Tsinghua Center for Life Sciences Peking UniversityBeijing 100871China Department of Chemistry and Institute for Biophysical Dynamics The University of ChicagoChicagoIL 60637USA Howard Hughes Medical Institute ChicagoIL 60637USA

CORRECTION TO:PROTEIN CELL(2021)HTTPS://***/10.1007/S13238-021-00837-8 In the original publication of the article figure 1 is incorrectly *** correct Figure 1 is provided in this *** ACCESS This article is licensed under a Creative Commons Attribution 4.0 International License,which permits use,sharing,adaptation,distribution and reproduction in any medium or format,as long as you give appropriate credit to the original author(s)and the source,provide a link to the Creative Commons licence,and indicate if changes were *** images or other third party material in this article are included in the article's Creative Commons licence,unless indicated otherwise in a credit line to the *** material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use,you will need to obtain permission directly from the copyright *** view a copy of this licence,visit https://***/licenses/by/4.0/.

关键词： ACCESS HTTPS Figure

ELL3通过LINE-1调节小鼠细胞原始多能性

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科学通报 2023年第33期68卷 4433-4435页

作者：常展赫朱学昊高绍荣高亚威同济大学生命科学与技术学院同济大学附属东方医院再生医学研究所上海市信号转导与疾病研究重点实验室上海200092 同济大学细胞干性与命运编辑前沿科学中心上海200092

人类基因组中有超过50%的序列是转座子序列(transposon elements,TE).转座子又称跳跃基因,是一类可以在基因组中进行自我复制和粘贴的DNA元件[1].转座子主要分为DNA转座子和反转录转座子,其中反转录转座子会先转录产生RNA,再反转录成DN... 详细信息

人类基因组中有超过50%的序列是转座子序列(transposon elements,TE).转座子又称跳跃基因,是一类可以在基因组中进行自我复制和粘贴的DNA元件[1].转座子主要分为DNA转座子和反转录转座子,其中反转录转座子会先转录产生RNA,再反转录成DNA来完成转座.长散在重复序列(long interspersed nuclear elements-1,LINE-1)是哺乳动物基因组中最丰富的转座元件家族之一,在人类基因组中占比大约为17%[2].

关键词：反转录转座子人类基因组跳跃基因自我复制转座元件多能性小鼠细胞