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Polysaccharide of Alocasia cucullata Exerts Antitumor Effect by Regulating Bcl-2, Caspase-3 and ERK1/2 Expressions during Long-Time Administration

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Chinese Journal of Integrative Medicine 2024年第1期30卷 52-61页

作者： ZHOU Qi-chun XIAO shi-lin lin Ru-kun LI Chan CHEN Zhi-jie CHEN Yi-fei LUO Chao-hua MO Zhi-xian lin Ying-bo School of Traditional Chinese Medicine Southern Medical UniversityGuangzhou(510515)China Clinical and Basic Research Team of TCM Prevention and Treatment of Non-Small Cell Lung Cancer the Second Clinical College of Guangzhou University of Chinese MedicineGuangzhou(510120)China Department of Chinese Medicine Zhujiang Hospital Affiliated to Southern Medical UniversityGuangzhou(510282)China Department of Pharmacy Jiangmen Central HospitalJiangmen(529000)China

Objective: To study the in vitro and in vivo antitumor effects of the polysaccharide of Alocasia cucullata(PAC) and the underlying mechanism. Methods: B16F10 and 4T1 cells were cultured with PAC of 40 μg/m L, and PAC was withdrawn after 40 days of administration. The cell viability was detected by cell counting kit-8. The expression of Bcl-2 and Caspase-3 proteins were detected by Western blot and the expressions of ERK1/2 mRNA were detected by quantitative real-time polymerase chain reaction(qRT-PCR). A mouse melanoma model was established to study the effect of PAC during long-time administration. Mice were divided into 3 treatment groups: control group treated with normal saline, positive control group(LNT group) treated with lentinan at 100 mg/(kg·d), and PAC group treated with PAC at 120 mg/(kg·d). The pathological changes of tumor tissues were observed by hematoxylin-eosin staining. The cell apoptosis of tumor tissues was detected by TUNEL staining. Bcl-2 and Caspase-3 protein expressions were detected by immunohistochemistry, and the expressions of ERK1/2, JNK1 and p38 mRNA were detected by qRT-PCR. Results: In vitro, no strong inhibitory effects of PAC were found in various tumor cells after 48 or 72 h of administration. Interestingly however, after 40 days of cultivation under PAC, an inhibitory effect on B16F10 cells was found. Correspondingly, the long-time administration of PAC led to downregulation of Bcl-2 protein(P <0.05), up-regulation of Caspase-3 protein(P<0.05) and ERK1 mRNA(P<0.05) in B16F10 cells. The above results were verified by in vivo experiments. In addition, viability of B16F10 cells under long-time administration culture in vitro decreased after drug withdrawal, and similar results were also observed in 4T1 cells. Conclusions: Long-time administration of PAC can significantly inhibit viability and promote apoptosis of tumor cells, and had obvious antitumor effect in tumor-bearing mice.

关键词： polysaccharide of Alocasia cucullata tumor cells Bcl-2 Caspase-3 ERK1/2 Chinese medicine

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Dynamic manipulation of multimodal emission in Er^(3+)-activated non-core-shell structure for optical thermometry and information security

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Rare Metals 2024年第4期43卷 1702-1712页

作者： Xuan Liu Zi-Xia lin Yin-Biao shi Xiao-Yan Wang Ming-Ye Ding Xiao-Fei Yang College of Science Nanjing Forestry UniversityNanjing210037China Testing Center Yangzhou UniversityYangzhou225009China

Regulating luminescent dynamics of lanthanide-based luminescent materials via external stimuli is of great significance in the fields of optical thermometry and high-level ***,it is still a huge challenge to realize multimodal emissions with tunable color outputs from a single activator in simple structures via smart dynamic control of photon transition ***,we present a mechanistic strategy to achieve multimodal luminescence of Er^(3+)activators with color-switchable outputs in a non-core-shell *** the control of excitation dynamics(λ_(ex)=980,808,1532,377 nm),the population among the intermediate en ergy levels of Er^(3+)and the interaction between Er^(3+)and Yb^(3+)could be precisely modulated through energy transfer and migration processes,leading to the generation of colortunable multimodal luminescence upon diverse excitation modes(non-steady-state,single-/dual-wavelength steady,thermal activation).Inspired by its special luminescent performance,the as-obtained material exhibits great potential in noncontact thermometry,multimodal anticounterfeiting,and high-capacity information encryption by performing a series of proof-of-concept *** findings might provide a conceptual model to modulate the luminescent dynamics in a simple-structured system for the generation of color-adjustable multimodal emissions,which is convenient for the development of advanced luminescent materials toward versatile cuttingedge applications.

关键词： Lanthanide ions Multimodal emission Upconversion Downshifting Anti-counterfeiting Luminescent thermometry

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The value of squamous cell carcinoma antigen in the prognostic evaluation, treatment monitoring and follow-up of patients with penile cancer

The value of squamous cell carcinoma antigen in the prognost...

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第十五届全国泌尿外科学术会议

作者： yao Zhu, Ding-Wei Ye, Xu-Dong yao, shi-lin Zhang, Bo Dai, Hai-Liang Zhang, Yi-Jun Shen 肿瘤医院

Purpose: The aim of this study was to examine whether squamous cell carcinoma antigen (SCCAg) has significant value in the prognostic evaluation, treatment monitoring and follow-up of patients with penile cancer. Materials and Methods: The measurement of serum SCCAg was prospectively performed in 63 patients with penile squamous cell carcinoma from 2005 to 2007.

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Predicting inguinal lymph node metastases:a new nomogram based on several molecular markers in primary tumor

Predicting inguinal lymph node metastases:a new nomogram bas...

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第十五届全国泌尿外科学术会议

作者： yao Zhu, Ding-Wei Ye, Xu-Dong yao, shi-lin Zhang, Bo Dai, Hai-Liang Zhang, Yi-Jun Shen 肿瘤医院

Purpose: The presence of lymph node metastasis is hard to define in penile squamous cell carcinoma. The goal of this study was to construct a new predictive nomogram based on the expression of p53, Ki-67, epithelial c...

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Casein kinase-2 inhibition promotes retinal ganglion cell survival after acute intraocular pressure elevation

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Neural Regeneration Research 2024年第5期19卷 1112-1118页

作者： Meng Wang shi-Qi yao yao Huang Jia-Jian Liang Yanxuan Xu Shaowan Chen Yuhang Wang Tsz Kin Ng Wai Kit Chu Qi Cui ling-Ping Cen Joint Shantou International Eye Center of Shantou University and The Chinese University of Hong Kong ShantouGuangdong ProvinceChina Shantou University Medical College ShantouGuangdong ProvinceChina Beijing Tongren Eye Center Beijing Tongren HospitalCapital Medical UniversityBeijingChina Department of Ophthalmology and Visual Sciences The Chinese University of Hong KongHong Kong Special Administrative RegionChina

Intraocular pressure elevation can induce retinal ganglion cell death and is a clinically reversible risk factor for glaucoma,the leading cause of irreversible *** previously demonstrated that casein kinase-2 inhibition can promote retinal ganglion cell survival and axonal regeneration in rats after optic nerve *** investigate the underlying mechanism,in the current study we increased the intraocular pressure of adult rats to 75 mmHg for 2 hours and then administered a casein kinase-2 inhibitor(4,5,6,7-tetrabromo-2-azabenzimidazole or 2-dimethylamino-4,5,6,7-tetrabromo-1H-benzimidazole)by intravitreal *** found that intravitreal injection of 4,5,6,7-tetrabromo-2-azabenzimidazole or 2-dimethylamino-4,5,6,7-tetrabromo-1H-benzimidazole promoted retinal ganglion cell survival and reduced the number of infiltrating *** analysis showed that the mitogen activated protein kinase signaling pathway was involved in the response to intraocular pressure elevation but was not modulated by the casein kinase-2 ***,casein kinase-2 inhibition downregulated the expression of genes(Cck,Htrsa,Nef1,Htrlb,Prph,Chat,Slc18a3,Slc5a7,Scn1b,Crybb2,Tsga10ip,and Vstm21)involved in intraocular pressure *** data indicate that inhibition of casein kinase-2 can enhance retinal ganglion cell survival in rats after acute intraocular pressure elevation via macrophage inactivation.

关键词： casein kinase-2 glaucoma intraocular pressure elevation macrophages retinal ganglion cells

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AHFO-based soil water content sensing technology considering soil-sensor thermal contact resistance

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Journal of Rock Mechanics and Geotechnical Engineering 2024年第7期16卷 2715-2731页

作者： Mengya Sun Peng Wu Bin shi Jin Liu Jie Liu Juncheng yao Yipin Lu Yunqiang Wang Xiaoyan Li School of Earth Sciences and Engineering Hohai UniversityNanjing210098China School of Earth Sciences and Engineering Nanjing UniversityNanjing210023China Department of Architecture and Civil Engineering City University of Hong Kong999077Hong KongChina State Key Laboratory of Loess and Quaternary Geology Institute of Earth EnvironmentChinese Academy of SciencesXi’an710061China

The actively heated fiber-optic(AHFO)technology has become emerged as a research focus due to its advantages of distributed,real-time measurement and good *** attributes have led to the gradual application of AHFO technology to the water content measurement of in situ ***,all existing in situ applications of AHFO technology fail to consider the effect of soilesensor contact quality on water content measurements,limiting potential for the wider application of AHFO *** address this issue,the authors propose a method for determining the soilesensor thermal contact resistance based on the principle of an infinite cylindrical heat *** is then used to establish an AHFO water content measurement technology that considers the thermal contact *** reliability and validity of the new measurement technology are explored through a laboratory test and a field case study,and the spatial-temporal evolution of the soil water content in the case is *** results demonstrate that method for determining the soilesensor thermal contact resistance is highly effective and applicable to all types of *** method requires only the moisture content,dry density,and thermal response of the in situ soil to be *** the field case,the measurement error of soil water content between the AHFO method,which takes into account the thermal contact resistance,and the neutron scattering method is only *** water content of in situ soil exhibits a seasonal variation,with an increase in spring and autumn and a decrease in summer and ***,the response of shallow soils to precipitation and evaporation is *** findings contribute to the enhancement of the accuracy of the AHFO technology in the measurement of the water content of in situ soils,thereby facilitating the dissemination and utilization of this technology.

关键词： Soil water content Actively heated fiber-optic(AHFO) technology Soilesensor thermal contact resistance Reliability In situ application

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Nanochaperones Based on Hydrophobic Interaction and Coordination Inhibit Protein Misfolding and Fibrillation

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Chinese Journal of Polymer Science 2024年第11期 1710-1718页

作者： Hui Wang lin-lin Xu lin-Qi shi Ru-Jiang Ma Key Laboratory of Functional Polymer Materials of Ministry of Education Institute of Polymer Chemistry State Key Laboratory of Medicinal Chemical BiologyFrontiers Science Center for New Organic Matter College of Chemistry Nankai University Haihe Laboratory of Sustainable Chemical Transformations

Amyloidosis is characterized by the deposition of fibrillar aggregates, with a specific peptide or protein as the primary component, in affected tissues or organs. Excessive proliferation and deposition of amyloid fibrils can cause organismal dysfunction and lethal pathological outcomes associated with amyloidosis. In this study, a nanochaperone(nChap-NA) was developed to inhibit protein misfolding and fibrillation by simulating the function of natural molecular chaperones. The nanochaperone was prepared by self-assembly of two block copolymers PEG-b-PCL and PCL-b-P(NIPAM-co-AANTA), which had a phase-separated surface consisting of hydrophobic PNIPAM microdomains with coordinative NTA(Zn) moieties and hydrophilic PEG chains. The hydrophobic interaction of the PNIPAM microdomain and the coordination of NTA(Zn) synergistically work together to effectively trap the amyloid monomer and block its fibrillation site. Insulin and human islet amyloid polypeptide(hIAPP) were used as model proteins to investigate the nanochaperone's inhibition of amyloid misfolding and fibrillation. It was proved that the nanochaperone could stabilize the natural conformation of the trapped insulin and hIAPP, and effectively inhibit their fibrillation. In vivo study demonstrated that the nanochaperone could effectively preserve the bioactivity of insulin and reduce the cytotoxicity caused by hIAPP aggregation. This study may provide a promising strategy for the prophylactic treatment of amyloidosis.

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基于R软件单组率Meta分析研究9种中医体质的人群分布

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安徽中医药大学学报 2021年第4期40卷 99-104页

作者：姚实林安徽中医药大学科技处安徽合肥230012

目的对中国人群中医体质类型分布情况进行系统评价,为9种中医体质类型全国常模的建立提供循证医学证据。方法在中国知网、万方数据知识服务平台、维普网、PubMed数据库、Cochrane数据库中检索9种中医体质类型流行病学调查的文献,采用R... 详细信息

目的对中国人群中医体质类型分布情况进行系统评价,为9种中医体质类型全国常模的建立提供循证医学证据。方法在中国知网、万方数据知识服务平台、维普网、PubMed数据库、Cochrane数据库中检索9种中医体质类型流行病学调查的文献,采用R软件单组率Meta分析对9种中医体质类型的构成比进行合并,根据构成比的潜在影响因素的Meta回归分析结果进行亚组分析。结果共纳入19篇文献,总样本量为175331。Meta分析结果显示,平和质、气虚质、阳虚质、阴虚质、痰湿质、湿热质、血瘀质、气郁质、特禀质的构成比分别为0.29(95%CI:0.23~0.36)、0.15(95%CI:0.13~0.18)、0.15(95%CI:0.13~0.18)、0.07(95%CI:0.05~0.10)、0.09(95%CI:0.07~0.12)、0.08(95%CI:0.06~0.10)、0.04(95%CI:0.03~0.05)、0.06(95%CI:0.05~0.08)、0.03(95%CI:0.02~0.04),“男女性别比”是阴虚质构成比的影响因素,“是否兼夹”是阴虚质、痰湿质、湿热质、血瘀质构成比的影响因素,“地区”是平和质、血瘀质、气郁质、特禀质构成比的影响因素。结论中国人群平和质比例不足1/3,气虚质、阳虚质是最常见的偏颇体质,地域是人群体质的主要影响因素,中医体质类型调查报告有待规范。

关键词：中医体质体质分类 R软件 Meta分析 Meta回归

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Bending short DNAs as transversely isotropic rings in series

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Acta Mechanica Sinica 2024年第7期40卷 206-212页

作者： Chenyu shi Meicheng yao Bin Chen Department of Engineering Mechanics Zhejiang UniversityHangzhou 310027China Key Laboratory of Soft Machines and Smart Devices of Zhejiang Province Hangzhou 310027China

Despite the significance of the high flexibility exhibited by short DNAs,there remains an incomplete understanding of their anomalous persistence *** this study,we propose to model each basic unit of gene sequences within short DNAs as a transversely isotropic *** comprehensive model analysis successfully replicates the observed high flexibility of short DNAs and also displays the impact of sequence dependence,aligning with experimental ***,our analysis suggests that the bending behavior of short DNAs can be effectively described by the Timoshenko beam theory with the consideration of shear.

关键词： DNA Persistence length Timoshenko beam theory Transversely isotropic Bending

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Left ventricular thrombosis caused cerebral embolism during venoarterial extracorporeal membrane oxygenation support: A case report

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World Journal of Clinical Cases 2024年第5期12卷 973-979页

作者： yao-Bang Bai Feng Zhao Zhen-Hua Wu Guo-Ning shi Nan Jiang Intensive Care Unit Department of Cardiac SurgeryTianjin Chest HospitalTianjin 300222China Department of Cardiac Surgery Tianjin Chest HospitalTianjin 300222China

BACKGROUND Venoarterial(VA)extracorporeal membrane oxygenation(ECMO),an effective short-term circulatory support method for refractory cardiogenic shock,is widely ***,retrospective analyses have shown that VA-ECMO-assisted cases were associated with a relatively high mortality rate of approximately 60%.Embolization in important organs caused by complications of left ventricular thrombosis(LVT)during VA-ECMO is also an important *** the incidence of LVT during VA-ECMO is not high,the consequences of embolization are *** SUMMARY A 37-year-old female patient was admitted to hospital because of fever for 4 d and palpitations for 3 *** excluding the diagnosis of coronary heart disease,we established a diagnosis of“clinically explosive myocarditis”.The patient still had unstable hemodynamics after drug treatment supported by VA-ECMO,with heparin for *** day 4 of ECMO support,a left ventricular thro-mbus attached to the papillary muscle root of the mitral valve was found by transthoracic *** ventricular decompression was performed and ECMO was successfully removed,but the patient eventually died of multiple cerebral *** LVT with high mobility during VA-ECMO may cause embolism in important ***,a"wait and see"strategy should be avoided.

关键词： Venoarterial extracorporeal membrane oxygenation Left ventricular thrombosis Cerebral embolism Magnetic Resonance Imaging Therapy Case report

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