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Feedback enhanced tumor targeting delivery of albumin-based nanomedicine to amplify photodynamic therapy by regulating AMPK signaling and inhibiting GSTs

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Chinese Chemical Letters 2024年第8期35卷 481-485页

作者： Jiaqi Huang Renjiang Kong yanmei li Ni Yan Yeyang Wu Ziwen Qiu Zhenming Lu Xiaona Rao Shiying li Hong Cheng School of Biomedical Engineering&Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering Southern Medical UniversityGuangzhou 510515China School of Pharmaceutical Sciences Guangzhou Medical UniversityGuangzhou 511436China

Oxidative therapies receive a limited antitumor efficiency due to the insufficient reactive oxygen species(ROS)levels at focal sites and the evolvement of antioxidant defense ***,we develop an albumin-based nanomedicine to co-deliver chlorin e6(Ce6)and COH-SR4(CS),which can simultaneously enhance the yield and lethality of intracellular ROS for amplified photodynamic therapy(PDT).In which,CS acts as both an activator of AMP-activated protein kinase(AMPK)and an inhibitor of glutathione S-transferases(GSTs).Benefiting from it,the prepared HSA-Ce6@COH-SR4(HCCS)enables positive feed-back uptake by promoting AMPK phosphorylation,leading to rapid and extensive tumor accumulation of *** a result,HCCS obviously increases the ROS production to elevate intracellular oxidative ***,HCCS can inhibit GSTs to disturb the antioxidant defense system of tumor cells,intensifying the oxidative damage of ***,the PDT of HCCS is significantly strengthened by improving the ROS yield and lethality,which greatly declines the proliferation of breast cancer in *** study may open a window in the development of drug co-delivery system for enhanced oxidative therapy of tumors.

关键词： Human serum albumin Drug delivery Reactive oxygen species Positive feedback uptake Oxidative damage

Safety assessment of a novel marine multi-stress-tolerant yeast Meyerozyma guilliermondii GXDK6 according to phenotype and whole genome-sequencing analysis

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Food Science and Human Wellness 2024年第4期13卷 2048-2059页

作者： Xueyan Mo Mengcheng Zhou yanmei li lili Yu Huashang Bai Peihong Shen Xing Zhou Haojun Zhu Huijie Sun Ru Bu Chengjian Jiang State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangxi Research Center for Microbial and Enzyme Engineering TechnologyCollege of Life Science and TechnologyGuangxi UniversityNanning 530004China National Engineering Research Center for Non-Food Biorefinery State Key Laboratory of Non-Food Biomass and Enzyme TechnologyGuangxi Research Center for Biological Science and TechnologyGuangxi Academy of SciencesNanning 530007China Guangxi Key Laboratory for Green Processing of Sugar Resources College of Biological and Chemical EngineeringGuangxi University of Science and TechnologyLiuzhou 545006China

The application of microorganisms as probiotics is limited due to lack of safety ***,a novel multi-stress-tolerant yeast Meyerozyma guilliermondii GXDK6 with aroma-producing properties was identified from marine mangrove *** safety and probiotic properties were assessed in accordance with phenotype and whole-genome sequencing *** showed that the genes and phenotypic expression of related virulence,antibiotic resistance and retroelement were rarely *** morphogenesis genes(SIT4,HOG1,SPA2,ERK1,ICL1,CST20,HSP104,TPS1,and RHO1)and phospholipase secretion gene(VPS4)were *** hyphae and phospholipase were *** one retroelement(Tad1-65_BG)was *** biogenic amines(BAs)encoding genes were absent,except for spermidine synthase(JA9_002594),spermine synthase(JA9_004690),and tyrosine decarboxylase(inx).The production of single BAs and total BAs was far below the food-defined ***6 had no resistance to common antifungal *** enzymes,such as gelatinase,DNase,hemolytic,lecithinase,and thrombin were *** toxicity test with mice demonstrated that GXDK6 is ***6 has a good reproduction ability in the simulation gastrointestinal ***6 also has a strong antioxidant ability,β-glucosidase,and inulinase *** sum up,GXDK6 is considered as a safe probiotic for human consumption and food fermentation.

关键词： Meyerozyma guilliermondii Safety assessment Probiotics Marine mangrove microorganisms Whole-genome sequencing analysis

Recent advances in chemical protein synthesis:method developments and biological applications

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Science China Chemistry 2024年第4期67卷 1060-1096页

作者： Suwei Dong Ji-Shen Zheng Yiming li Huan Wang Gong Chen Yongxiang Chen Gemin Fang Jun Guo Chunmao He Honggang Hu Xuechen li yanmei li Zigang li Man Pan Shan Tang Changlin Tian Ping Wang Bian Wu Chuanliu Wu Junfeng Zhao Lei liu State Key Laboratory of Natural and Biomimetic Drugs and School of Pharmaceutical SciencesPeking UniversityBeijing 100191China Division of life Sciences and Medicine University of Science and Technology of ChinaHefei 230027China School of Food and Biological Engineering Engineering Research Center of Bio-processMinistry of EducationHefei University of TechnologyHefei 230009China School of Chemistry and Chemical Engineering Nanjing UniversityNanjing 210023China State Key Laboratory of Elemento-Organic Chemistry Nankai UniversityTianjin 300071China Department of Chemistry Tsinghua UniversityBeijing 100084China Institute of Health Sciences Anhui UniversityHefei 230601China College of Chemistry Central China Normal UniversityWuhan 430079China School of Chemistry and Chemical Engineering South China University of TechnologyGuangzhou 510640China School of Medicine or Institute of Translational Medicine Shanghai UniversityShanghai 200444China Department of Chemistry The University of Hong KongHong Kong 999077China Shenzhen Graduate School Peking UniversityShenzhen 518055China School of Chemistry and Chemical Engineering Shanghai Jiao Tong UniversityShanghai 200240China School of life Sciences University of Science and Technology of ChinaHefei 230026China Department of Chemical Biology Shanghai Jiao Tong UniversityShanghai 200240China Institute of Microbiology Chinese Academy of SciencesBeijing 100101China Department of Chemistry Xiamen UniversityXiamen 361005China School of Pharmaceutical Science Guangzhou Medical UniversityGuangzhou 511436China

The central dogma of modern biology underscores the pivotal roles proteins play in diverse biological processes,the study of which necessitates advanced methods to produce proteins with precision and *** protein synthesis,a powerful approach utilizing chemical reactions for the de novo construction of structurally accurate proteins,has emerged as a transformative tool for studying proteins and generating protein derivatives/mimics inaccessible by natural biological machinery,including post-translationally modified proteins,proteins comprised of unnatural amino acids,as well as mirror-image *** review summarizes recent strides in synthetic method developments for chemical protein synthesis,including innovative techniques in solid-phase peptide synthesis,the challenges presented by difficult sequences in either synthesis or folding and the exploration of novel ligation reactions using both chemical and enzymatic ***,the review also delves into newly developed protocols for site-selective protein modifications and the generation of stapled or macrocyclized peptides/miniproteins,highlighting the power of chemical methods to make structurally diverse *** applications of synthetic proteins in investigating post-translational modifications(phosphorylation,lipidation,glycosylation,ubiquitination,etc.),mirror-image biological processes and drug development are further ***,these topics provide a comprehensive overview of the current landscape of chemical protein synthesis.

关键词： chemical protein synthesis solid-phase peptide synthesis ligation reactions post-translational modifications mirror-image proteins peptide drugs

Elevated Kallistatin promotes the occurrence and progression of non-alcoholic fatty liver disease

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Signal Transduction and Targeted Therapy 2024年第4期9卷 1730-1744页

作者： Zhenzhen Fang Gang Shen Yina Wang Fuyan Hong Xiumei Tang Yongcheng Zeng Ting Zhang Huanyi liu yanmei li Jinhong Wang Jing Zhang Anton Gao Weiwei Qi Xia Yang Ti Zhou Guoquan Gao Department of Biochemistry Zhongshan School of MedicineSun Yat-Sen UniversityGuangzhou 510080China Department of VIP Medical Center the Third Affiliated Hospital of Sun Yat-Sen UniversityGuangzhou 510080China Physical Examination Center the Third Affiliated Hospital of Sun Yat-Sen UniversityGuangzhou 510080China Department of Clinical Laboratory Guangzhou First People's HospitalGuangzhou 510080China Guangdong Key Laboratory of liver Disease Research the Third Affiliated Hospital of Sun Yat-Sen UniversityGuangzhou 51008China Department of Health Sciences College of Health SolutionsArizona State UniversityTempeUSA Guangdong Engineering&Technology Research Center for Gene Manipulation and Biomacromolecular Products Sun Yat-Sen UniversityGuangzhou 510080China Guangdong Province Key Laboratory of Diabetology Guangzhou 510o80China Guangdong Province Key Laboratory of Brain Function and Disease Zhongshan School of MedicineSun Yat-Sen UniversityGuangzhou 510080China

Non-alcoholic fatty liver disease(NAFLD)is the most common chronic liver disease worldwide,and the development of nonalcoholic steatohepatitis(NASH)might cause irreversible hepatic ***(HLP)is the leading risk factor for *** study aims to illuminate the causative contributor and potential mechanism of Kallistatin(KAL)mediating HLP to NAFLD.221 healthy control and 253 HLP subjects,62 healthy control and 44 NAFLD subjects were *** plasma KAL was significantly elevated in HLP subjects,especially in hypertriglyceridemia(HTG)subjects,and positively correlated with liver ***,KAL levels of NAFLD patients were significantly *** transgenic mice induced hepatic steatosis,inflammation,and fibrosis with time and accelerated inflammation development in high-fat diet(HFD)*** contrast,KAL knockout ameliorated steatosis and inflammation in high-fructose diet(HFruD)and methionine and choline-deficient(MCD)diet-induced NAFLD ***,KAL induced hepatic steatosis and NASH by down-regulating adipose triglyceride lipase(ATGL)and comparative gene identification 58(CGl-58)by LRP6/Gas/PKA/GSK3βpathway through down-regulating peroxisome proliferator-activated receptor(PPARy)and up-regulating kruppel-like factor four(KLF4),***-58 is bound to NF-kB p65 in the cytoplasm,and diminishing CGl-58 facilitated p65 nuclear translocation and TNFa ***,hepatic CGl-58-overexpress reverses NASH in KAL transgenic ***,free fatty acids up-regulated KAL against thyroid hormone in ***,Fenofibrate,one triglyceride-lowering drug,could reverse hepatic steatosis by down-regulating *** results demonstrate that elevated KAL plays a crucial role in the development of HLP to NAFLD and may be served as a potential preventive and therapeutic target.

关键词： GSK3β inflammation elevated

Application Value of NLR,PLR,LMR,HEART score,and POCT in Early Warning and Accurate Graded Diagnosis of High-Risk Chest Pain in Emergency Medicine

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Journal of Clinical and Nursing Research 2024年第4期8卷 93-98页

作者： Shaochang Ma Chunhua lin yanmei li Yan Chen Guohui Zhang Guandu District People’s Hospital Kunming 650200Yunnan ProvinceChina Guanshang Street Health Service Center Guandu DistrictKunming 650200Yunnan ProvinceChina

Objective: To evaluate the application value of neutrophils/lymphocytes (NLR), platelets/lymphocytes (PLR), lymphocytes/monocytes (LMR), HEART (history, electrocardiogram, age, risk factors, and troponin) score, and point-of- care testing (POCT) in the early warning and precise diagnosis of high-risk chest pain in emergency medicine. Methods: A total of 157 patients with acute chest pain who were admitted to the emergency department and chest pain treatment unit of our hospital between August 2022 and September 2023 were selected. Rapid testing of bedside myocardial markers (ultrasensitive troponin (hs-cTnI), myoglobin (MYO), creatine kinase isoenzyme (CK-MB), D-dimer (D-Dimer), and N-terminal B-type natriuretic peptide precursor (NT-proBNP)) was performed on the patients using a POCT device (ThermoKing BioMQ60proB). A HEART score was used to classify the patients into low (n = 53), intermediate (n = 59), and high-risk (n = 45) groups, and the NLR, PLR, and LMR were calculated. The NLR, PLR, and LMR values were compared among the three groups of patients, and the optimal cutoff values as well as sensitivity and specificity were determined based on receiver operating characteristic (ROC) analysis. Results: The HEART scores of patients in the low-risk, intermediate-risk, and high-risk groups were (2.72 ± 0.24), (4.75 ± 0.56), and (5.32 ± 0.73) respectively, and the differences were statistically significant (P < 0.05). Compared with the low-risk group, the intermediate-risk group and high-risk group had a significantly higher NLR and PLR, and a significantly lower LMR;the high-risk group had higher NLR and PLR and lower values of LMR as compared to the other two groups, and the difference was statistically significant (P < 0.05). The ROC curves suggested that the area under the curve, sensitivity, and specificity of the combined diagnosis of NLR, PLR, LMR, HEART score, and POCT were greater than those of LR, PLR, and LMR with HEART score and POCT alone. Conclusion: Th

关键词： NLR PLR LMR POCT myocardial markers HEART score Emergency high-risk chest pain

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面向工业目标检测的密集控制阀零件数据集

光学精密工程 2024年第8期32卷 1241-1251页

作者：王琳毅白静李彦梅李文静北方民族大学计算机科学与工程学院宁夏银川750021 六盘山实验室宁夏银川750021 北方民族大学图像图形智能处理国家民委重点实验室宁夏银川750021

工业生产中的自动化智能化离不开自动目标检测,而高准确性的自动目标检测则依赖于与实际场景相适应的数据集。本文针对工业实际场景,发布了一个密集控制阀零件数据集,命名为PD4CV(Part Detection for Control Valve)2023。该数据集的图... 详细信息

工业生产中的自动化智能化离不开自动目标检测,而高准确性的自动目标检测则依赖于与实际场景相适应的数据集。本文针对工业实际场景,发布了一个密集控制阀零件数据集,命名为PD4CV(Part Detection for Control Valve)2023。该数据集的图像全部来源于控制阀生产车间,图像采集完成后,首先对数据集图片进行预处理操作,接着对数据集图片中的零件目标进行标注,然后再对数据集图片进行训练集、验证集以及测试集的划分。PD4CV2023数据集共涵盖9类零件,包括510张工盘图像和15 015个零件样本,平均每张图像含有约29个零件样本。与现有的目标检测数据集相比,该数据集具有零件摆放密集、遮挡,零件尺寸差异大,部分零件外形相似,零件样本数量不均衡等特点。最后,在不同类型数据集上的预训练对比实验表明,一般场景数据集、特定工业场景数据集只适用于一般和特定任务,而代表实际控制阀生产工况下的PD4CV2023数据集,可用于控制阀零件目标检测,其具有其特殊性和不可替代性;一系列目标检测算法在该数据集上的综合对比则验证了PD4CV2023数据集在一般性目标检测、多尺度目标检测、小规模、不均衡数据下目标检测中的有效性。PD4CV2023数据集可用于面向工业的目标检测的相关研究。

关键词：深度学习工业目标检测数据集控制阀零件

冷藏薄荷水喷雾对胸腔镜下肺叶切除术患者口渴症状和口腔舒适度的影响

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中国实用护理杂志 2024年第5期40卷 386-391页

作者：谢鹏李艳梅朱泉余雷孝感市中心医院手术麻醉科孝感432000 孝感市中心医院妇科孝感432000

目的探讨冷藏薄荷水喷雾在胸腔镜下肺叶切除术后患者中的应用效果,旨在为建立有效的口渴管理策略提供依据。方法采用随机对照临床研究方法,便利选取2022年5月至2023年5月孝感市中心医院进行胸腔镜下肺叶切除术的100例患者为研究对象,采... 详细信息

目的探讨冷藏薄荷水喷雾在胸腔镜下肺叶切除术后患者中的应用效果,旨在为建立有效的口渴管理策略提供依据。方法采用随机对照临床研究方法,便利选取2022年5月至2023年5月孝感市中心医院进行胸腔镜下肺叶切除术的100例患者为研究对象,采用随机对照表法分为试验组和对照组,每组50例。2组患者均给予常规麻醉苏醒期护理,在此基础上对照组采用冷藏纯净水(6~10℃)喷雾治疗,试验组采用冷藏薄荷水(6~10℃)喷雾治疗。比较2组患者的口渴程度、静息唾液流率、唇舌口腔黏膜湿润程度以及口腔舒适度。结果对照组患者男28例,女22例,年龄(58.30±16.64)岁;试验组患者男30例,女20例,年龄(58.66±16.68)岁。干预后1、2、4、6 h试验组口渴程度评分分别为(5.96±1.58)、(5.08±1.37)、(4.48±1.18)、(3.76±0.72)分,低于对照组的(6.78±1.04)、(5.60±1.09)、(5.10±1.16)、(4.52±1.09)分,差异均有统计学意义(t值为2.10~4.11,均P<0.05)。干预后2、4、6 h试验组静息唾液流率分别为(0.21±0.04)、(0.23±0.05)、(0.30±0.08)ml/min,高于对照组的(0.18±0.06)、(0.19±0.06)、(0.21±0.08)ml/min,差异均有统计学意义(t=2.31、3.22、6.57,均P<0.05)。干预后2、4、6 h试验组唇舌口腔黏膜湿润评分分别为(2.52±0.93)、(2.26±0.75)、(1.82±0.83)分,低于对照组的(2.98±0.84)、(2.88±0.85)、(2.30±0.76)分,差异均有统计学意义(t=2.59、3.87、2.38,均P<0.05)。干预后3、6 h试验组口腔舒适度评分分别为(4.54±0.39)、(5.68±1.67)分,高于对照组的(3.62±0.21)、(4.76±1.22)分,差异均有统计学意义(t=3.19、3.14,均P<0.05)。结论冷藏薄荷水喷雾能有效改善胸腔镜下肺叶切除术后患者口渴程度,提高口腔舒适度,为临床医护人员护理口渴患者提供新的思路。

关键词：肺肿瘤肺切除术口渴外科护理

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精神意蕴视角下风景写生创作体系构建研究

黑河学院学报 2024年第4期15卷 174-176,180页

作者：李燕梅福州外语外贸学院福建福州350202

风景写生从本质上而言并非是对于客观世界的客观描摹,而是一种主观性质的对于自然景观的体会与感悟。然而在当代的写生创作中,由于中西方艺术思想并存的缘故,致使许多创作者无法在写生创作中真正的赋予作品以生命力,尤其是在油画写生和... 详细信息

风景写生从本质上而言并非是对于客观世界的客观描摹,而是一种主观性质的对于自然景观的体会与感悟。然而在当代的写生创作中,由于中西方艺术思想并存的缘故,致使许多创作者无法在写生创作中真正的赋予作品以生命力,尤其是在油画写生和水彩画写生创作中体现的尤为明显。将精神意蕴引入到风景写生创作的研究之中,通过精神意蕴的引入而更好的展现当代风景写生作品的思想内涵和艺术生命,让风景写生创作不再是机械化的描绘,而是具有生命力的表达。

关键词：风景写生创作精神意蕴艺术体系形成过程

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面料拉伸性能与运动文胸防震功能的定量关系

纺织学报 2024年第1期45卷 161-167页

作者：盛欣洋陈晓娜卢娅娅李艳梅孙光武上海工程技术大学纺织服装学院上海201620

为探究面料拉伸性能与运动文胸防震功能之间的定量关系,选取6种不同针织面料,先分别测量不同面料拉伸率为30%时的弹性模量来表征其拉伸性能,然后将这6种面料作为罩杯部分用料制作文胸,其它部位为同种面料。以运动假人为实验对象,在其表... 详细信息

为探究面料拉伸性能与运动文胸防震功能之间的定量关系,选取6种不同针织面料,先分别测量不同面料拉伸率为30%时的弹性模量来表征其拉伸性能,然后将这6种面料作为罩杯部分用料制作文胸,其它部位为同种面料。以运动假人为实验对象,在其表面选取7个位置粘贴标记点,采用三维人体动作捕捉系统,采集实验对象在模拟10 km/h跑步过程中各标记点的坐标变化,并计算出各点在局部坐标系下的相对位移。以运动过程中运动文胸对乳房竖直位移的减小作用来表征其防震功能,通过曲线拟合与模型筛选得出面料拉伸性能与文胸防震功能的定量关系。结果表明:6款罩杯面料动态最大拉伸率均值为30%,说明实验中用拉伸率为30%时的弹性模量表征面料的拉伸性能较为合理。罩杯面料弹性模量与乳房竖直位移间存在显著负相关,即面料弹性模量越大,乳房竖直位移越小,表明文胸防震功能越好;幂函数模型可作为以罩杯面料弹性模量预测75C实验对象10 km/h跑步运动时乳房竖直位移的首选模型。未来可通过测量罩杯与乳房之间的静动态压力及乳房-罩杯整体刚度探究罩杯面料拉伸性能对文胸防震功能的影响机制。

关键词：运动文胸面料弹性模量防震功能乳房竖直位移曲线拟合运动假人